BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,900	+C	coding (616/3123 nt)	yegN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,897	1	.	C	97.0%	313.1 / 2.6	101	coding (613/3123 nt)	yegN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (40/58); total (41/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 123 total aligned reads displayed.

AAATCTCGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑C‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCC  >  NC_000913/2153805‑2153984

aaaTCTCGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTgccg                                                                                           <  1:3840217/93‑1 (MQ=255)

 aaTCTCGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTgccg‑c                                                                                         <  2:843702/93‑1 (MQ=255)

  aTCTCGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTgccg‑c‑c                                                                                       >  1:46847/1‑93 (MQ=255)

   tctcGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑cc                                                                                      >  1:4235933/1‑93 (MQ=255)

    ctcGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccc                                                                                     <  1:2388972/93‑2 (MQ=255)

    ctcGCAGAGTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccc                                                                                     <  1:2388971/93‑2 (MQ=255)

      cGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccctc                                                                                   <  2:3905727/93‑4 (MQ=255)

      cGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccctc                                                                                   <  2:1039906/93‑4 (MQ=255)

      cGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccctc                                                                                   >  1:4730474/1‑90 (MQ=255)

       gCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccctcg                                                                                  <  1:968906/93‑5 (MQ=255)

       gCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑c‑ccctcg                                                                                  <  2:3267605/93‑5 (MQ=255)

          gATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGcc                                                                                >  2:497233/1‑93 (MQ=255)

          gATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGcc                                                                                >  2:4579831/1‑93 (MQ=255)

          gATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGcc                                                                                >  1:769966/1‑93 (MQ=255)

          gATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGcc                                                                                >  1:1164917/1‑93 (MQ=255)

           aTTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCt                                                                               <  2:686935/93‑1 (MQ=255)

           aTTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCt                                                                               <  2:3923548/93‑1 (MQ=255)

            tttCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTg                                                                              >  2:349046/1‑93 (MQ=255)

               ccGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGAcc                                                                           >  2:4191202/1‑93 (MQ=255)

               ccGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGAcc                                                                           <  2:1435196/93‑1 (MQ=255)

               ccGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGAcc                                                                           >  2:1740974/1‑93 (MQ=255)

                 ggcggcggcCTGGTGACGCTTTGAGGCGGACAGCGTCCGGCTGTTCGCTTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAg                                                                         <  2:481598/93‑1 (MQ=255)

                 ggCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAg                                                                         >  1:1920806/1‑93 (MQ=255)

                  gcggcgGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGc                                                                        <  2:3567591/93‑1 (MQ=255)

                   cgtcgtCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCg                                                                       <  1:3232696/93‑1 (MQ=255)

                     tCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaa                                                                     >  2:1740622/1‑93 (MQ=255)

                      gcgCCTGGTGACGCTTTCCGGCGGTCAGCCTCCTGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaaa                                                                    <  1:1482563/91‑1 (MQ=255)

                      cGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaaa                                                                    <  1:3274954/93‑1 (MQ=255)

                      cGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaaa                                                                    >  1:3761960/1‑93 (MQ=255)

                      cGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaaa                                                                    <  1:542666/93‑1 (MQ=255)

                       ggCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAAc                                                                   <  2:1966102/93‑1 (MQ=255)

                       ggCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAAc                                                                   <  1:2378460/93‑1 (MQ=255)

                       cgacTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCCCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGaaaa                                                                   <  2:3613114/90‑2 (MQ=255)

                        gCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAAcc                                                                  <  2:3874848/93‑1 (MQ=255)

                          cTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGt                                                                <  1:108729/93‑1 (MQ=255)

                          cTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGt                                                                <  1:108730/93‑1 (MQ=255)

                          cTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGt                                                                >  1:3913583/1‑93 (MQ=255)

                          cTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑C‑CCTCGGCCTGACCAGCGAAACCGTg                                                               <  1:671289/93‑1 (MQ=255)

                           tGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTg                                                               <  2:1536588/93‑1 (MQ=255)

                            ggTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTgc                                                              <  2:227835/93‑1 (MQ=255)

                               gACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCa                                                           >  2:2207246/1‑93 (MQ=255)

                                  gCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCg                                                        >  2:2550103/1‑93 (MQ=255)

                                    tttCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTGACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAAACGTGCGCACCGcc                                                      >  2:619780/1‑93 (MQ=255)

                                      tCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCAt                                                    <  2:1877815/93‑1 (MQ=255)

                                         ggcggTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTAc                                                 <  1:2292666/93‑1 (MQ=255)

                                         ggcggTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTAc                                                 <  2:2063439/93‑1 (MQ=255)

                                         ggcggTCAGCGTCCGGCTGTTCGCGGCAATCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTAc                                                 <  2:1342677/93‑1 (MQ=255)

                                          gcggTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTAcc                                                <  1:4157442/93‑1 (MQ=255)

                                          gcggTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTAcc                                                <  2:797032/93‑1 (MQ=255)

                                           cggTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCg                                               <  1:389123/93‑1 (MQ=255)

                                             gTCAGCTTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGgc                                             <  2:1921557/93‑1 (MQ=255)

                                              tCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑C‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGgcgc                                           <  2:2004549/93‑1 (MQ=255)

                                               cAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGgcgc                                           >  2:3374379/1‑93 (MQ=255)

                                                 gCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTa                                         >  1:1439353/1‑93 (MQ=255)

                                                  cGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTaa                                        <  2:3902571/93‑1 (MQ=255)

                                                   gTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAAc                                       >  1:2778839/1‑93 (MQ=255)

                                                   gTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAAc                                       <  1:161083/93‑1 (MQ=255)

                                                   gTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑CTCCTCGGCCTGACCAGCGAAACCGTGCGCTCCGCCATTACCGGCGCTAAc                                       <  2:1523204/93‑1 (MQ=255)

                                                    tCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACg                                      <  1:2712839/93‑1 (MQ=255)

                                                    tCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACg                                      <  1:259006/93‑1 (MQ=255)

                                                    tCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACg                                      >  1:1861639/1‑93 (MQ=255)

                                                    tCCGGCTGATCGCGTCAAACTTAACGATCAGGTGATTGCCGAC‑ACTCGGCCTGACCAGCGAAACCGAGCGCGCCGCCATTACCGGCGCTATCg                                      <  2:4211729/93‑1 (MQ=255)

                                                      cGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGtt                                    <  2:200117/93‑1 (MQ=255)

                                                       ggCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTa                                   <  1:1124297/93‑1 (MQ=255)

                                                         gtGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAAc                                 <  1:1969850/92‑1 (MQ=255)

                                                          tGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACt                                >  1:3641483/1‑93 (MQ=255)

                                                          tGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACt                                >  2:443519/1‑93 (MQ=255)

                                                           gTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTc                               <  1:1416474/93‑1 (MQ=255)

                                                           gTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTc                               <  1:3451312/93‑1 (MQ=255)

                                                           gTTCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTc                               >  1:1780426/1‑93 (MQ=255)

                                                            ttCGCGTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCg                              <  2:3306315/93‑1 (MQ=255)

                                                               gcgTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCa                           <  1:3433391/93‑1 (MQ=255)

                                                               gcgTCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGAGAAACAGTGAGGACCGCCATTACCGGCGCTAAGGTTAAGTCGGCa                           >  2:1404043/1‑93 (MQ=255)

                                                                  tCAAACTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCaaaa                        <  2:2391362/93‑1 (MQ=255)

                                                                     aaCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAgga                     >  2:1514988/1‑92 (MQ=255)

                                                                     aaCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGt                     >  2:590025/1‑93 (MQ=255)

                                                                      aCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTa                    <  1:3934605/93‑1 (MQ=255)

                                                                      aCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTa                    >  2:237493/1‑93 (MQ=255)

                                                                      aCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTa                    <  2:2111172/93‑1 (MQ=255)

                                                                      aCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTa                    <  1:3934606/93‑1 (MQ=255)

                                                                      aCTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTa                    >  1:755768/1‑93 (MQ=255)

                                                                       cTTAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAg                   >  1:1286037/1‑93 (MQ=255)

                                                                        ttAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGc                  <  2:444588/93‑1 (MQ=255)

                                                                         tAACGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGcc                 <  2:2411563/93‑1 (MQ=255)

                                                                          aaCGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCt                <  1:271371/93‑1 (MQ=255)

                                                                          aaCGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCt                <  1:3604399/93‑1 (MQ=255)

                                                                          aaCGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCt                <  1:1900021/93‑1 (MQ=255)

                                                                           aCGCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTc               >  1:2976221/1‑93 (MQ=255)

                                                                            cGCTCAGGCGATTGCCGTC‑CTTCGGCCTGTCCAGCGAAACCGTGCGCACCGCAATTACCGGCGATAACGTTAACTCGGCAAAAGGTAGCCTCg              <  2:3431479/93‑1 (MQ=255)

                                                                             gCTCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGa             <  1:1570484/93‑1 (MQ=255)

                                                                               tCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCgcag           >  2:3766583/1‑90 (MQ=255)

                                                                               tCAGGCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACg           <  2:1020214/93‑1 (MQ=255)

                                                                                   gCGATTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGccc       <  2:3425368/93‑1 (MQ=255)

                                                                                     gATTGCCCCC‑CCTCGGCCTGACCAGCGAAACCGGGCGCACGGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGAAGCCTCGAGGGCCCtt     >  1:424853/1‑93 (MQ=255)

                                                                                      aTTGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTc    <  2:4343837/93‑1 (MQ=255)

                                                                                      aTTGCCG‑C‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTcc   >  1:4030487/1‑93 (MQ=255)

                                                                                       ttGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTcc   >  1:10628/1‑93 (MQ=255)

                                                                                       ttGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTcc   >  1:1792009/1‑93 (MQ=255)

                                                                                        tGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTccc  <  1:4570197/93‑1 (MQ=255)

                                                                                        tGCCGCC‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTccc  <  1:1697991/93‑1 (MQ=255)

AAATCTCGCAGATTTCCGGCGTCGGCCTGGTGACGCTTTCCGGCGGTCAGCGTCCGGCTGTTCGCGTCAAACTTAACGCTCAGGCGATTGCCG‑C‑CCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCC  >  NC_000913/2153805‑2153984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`