BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,243	+G	coding (869/957 nt)	yagS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,240	1	.	G	98.2%	356.4 / ‑0.7	112	coding (872/957 nt)	yagS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (67/43); total (69/43)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 130 total aligned reads displayed.

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTATGT  >  NC_000913/300148‑300326

tCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAt                                                                                          >  1:4719558/1‑93 (MQ=255)

tCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAt                                                                                          >  1:2053502/1‑93 (MQ=255)

 cAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑g                                                                                        >  1:4023202/1‑93 (MQ=255)

 cAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑g                                                                                        <  2:489992/93‑1 (MQ=255)

 cAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑g                                                                                        <  2:4278278/93‑1 (MQ=255)

   aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑ggg                                                                                      >  1:2486032/1‑93 (MQ=255)

     tttCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑ggggc                                                                                    <  1:853570/93‑3 (MQ=255)

       tCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑ggggcgc                                                                                  <  2:3374034/93‑5 (MQ=255)

         aTGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTg                                                                                 <  2:4487882/93‑1 (MQ=255)

          tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTgg                                                                                >  1:406149/1‑93 (MQ=255)

          tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTgg                                                                                <  1:1416321/93‑1 (MQ=255)

           gCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGc                                                                               >  2:3271984/1‑93 (MQ=255)

             cTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGa                                                                             >  2:3660850/1‑93 (MQ=255)

             cTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGa                                                                             >  2:3326146/1‑93 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAAc                                                                           >  2:2355416/1‑93 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTGTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAAc                                                                           <  2:2020616/93‑1 (MQ=255)

                 gCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAg                                                                         <  1:1250614/93‑1 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGc                                                                        <  2:3825046/93‑1 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGc                                                                        >  2:4479266/1‑93 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCg                                                                       <  1:1935660/93‑1 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCg                                                                       <  1:1185217/93‑1 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCg                                                                       >  2:1299189/1‑93 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      <  2:3804304/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      <  2:3804303/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      <  1:3176617/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      <  2:2758102/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCgtg                                                                     >  2:741098/1‑93 (MQ=255)

                     tCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCgtgt                                                                    >  2:2052925/1‑93 (MQ=255)

                     tCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGATTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgtg                                                                     >  1:2150569/1‑93 (MQ=255)

                         ttCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCat                                                                 <  1:1072563/93‑1 (MQ=255)

                         ttCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCat                                                                 >  2:3241120/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  1:288842/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  2:1624767/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  2:3043510/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  2:722514/1‑93 (MQ=255)

                              ccAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACg                                                            >  2:2575066/1‑93 (MQ=255)

                                aGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGc                                                          >  1:4208122/1‑93 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:4164855/1‑93 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGCGTTTAAAGGTGTTTTCAGCGGCGGGATGGGGCGCTGCGGAACAGCGTGGCATATACGGccg                                                        >  2:1172108/1‑92 (MQ=255)

                                   aCGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTg                                                       >  1:3663318/1‑93 (MQ=255)

                                    cGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgc                                                      <  1:4368807/93‑1 (MQ=255)

                                    cGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgc                                                      <  1:568143/93‑1 (MQ=255)

                                      gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  1:279117/1‑93 (MQ=255)

                                      gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  2:4743389/1‑93 (MQ=255)

                                         gCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcccc                                                 <  1:3732726/93‑1 (MQ=255)

                                         gCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcccc                                                 <  2:169316/93‑1 (MQ=255)

                                          cAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccccc                                                <  1:3241120/93‑1 (MQ=255)

                                          cAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccccc                                                <  2:129382/93‑1 (MQ=255)

                                          cAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccccc                                                <  2:129381/93‑1 (MQ=255)

                                           aaGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCt                                               >  1:3319306/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  2:3770619/93‑1 (MQ=255)

                                             gCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTgg                                             <  1:2581500/93‑1 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           >  1:1124781/1‑93 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           >  2:1076779/1‑93 (MQ=255)

                                               gTTCGCTGCGGCAACAGGATTTTAAAGGTTTTTTTTGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           <  1:1633341/93‑1 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  1:227482/1‑93 (MQ=255)

                                                  cgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGAGTGGCATATACGGCCTGCGCCCCCTGGGAtgg                                        >  1:1788893/1‑91 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  1:618700/1‑93 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       <  2:2160981/93‑1 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       <  1:2392567/93‑1 (MQ=255)

                                                    cttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCt                                      <  1:2928551/93‑1 (MQ=255)

                                                    cttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCt                                      >  1:4495978/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGCTAGCTg                                     >  2:514819/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  1:1362046/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     <  1:3445365/93‑1 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  2:750869/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  2:3771449/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  1:2461045/1‑93 (MQ=255)

                                                        gcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAga                                  >  1:1971334/1‑92 (MQ=255)

                                                        gcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGc                                  >  1:528595/1‑93 (MQ=255)

                                                         ccAACAGGAGTTTAAAGGCGTTTTCAGCGGTGGGATGGGGCGCTTGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCa                                 >  2:102672/1‑93 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               >  1:355990/1‑93 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               <  1:39907/93‑1 (MQ=255)

                                                            aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCGTGCGCCCCCTGGGATAGCTGAGCATcc                              <  2:2217943/93‑1 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑c                           >  1:2083035/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑c                           >  1:2776736/1‑93 (MQ=255)

                                                               ggAGTTTAAAGGTGTTTTCAGCGGTTGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑Ca                          >  1:3841118/1‑93 (MQ=255)

                                                               ggAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑Ca                          >  1:1280503/1‑93 (MQ=255)

                                                               ggAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑Ca                          >  2:1589656/1‑93 (MQ=255)

                                                                  gTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGcc                       <  2:2424688/93‑1 (MQ=255)

                                                                    ttAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTc                     <  2:4188329/93‑1 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGGTAGCTGAGCATCCG‑CAGCCTCCAt                  >  2:3661292/1‑93 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAAt                  >  2:4493943/1‑93 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAAt                  >  2:937712/1‑93 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAAt                  <  1:2233581/93‑1 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAAt                  >  1:364811/1‑93 (MQ=255)

                                                                         ggTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATgc                <  1:722103/93‑1 (MQ=255)

                                                                            tttttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGcc             <  1:1800133/92‑1 (MQ=255)

                                                                            gtTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGcc             <  1:1627387/93‑1 (MQ=255)

                                                                                 taGCGGTGGGATGGGGCGCTGGCGAACTGCGTGTCATATACGGCCTGCGCACTGTGGTATAGCTGAGCTTCCG‑CAGCCTCAATGCGCCAggga        <  2:544425/92‑2 (MQ=255)

                                                                                  aGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATGCGGCCTTCGCACCCCGGCATGGCTGCGC‑TCCGCCGGCCTCAATGGGCCCGGGCt       >  2:3514326/1‑93 (MQ=255)

                                                                                   gCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCtt      >  1:4606208/1‑93 (MQ=255)

                                                                                   gCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCtt      >  2:799339/1‑93 (MQ=255)

                                                                                    cGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTa     >  1:3767485/1‑93 (MQ=255)

                                                                                     ggTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAt    >  1:3071522/1‑93 (MQ=255)

                                                                                      gTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAtg   >  1:1013096/1‑93 (MQ=255)

                                                                                      gTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAtg   <  1:4104125/93‑1 (MQ=255)

                                                                                      gTGGGATGGGGAGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAtg   >  2:928403/1‑93 (MQ=255)

                                                                                       tGGGATGGGGGGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAtgt  <  1:1075812/93‑1 (MQ=255)

                                                                                       tGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTAtgt  <  1:1075811/93‑1 (MQ=255)

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCG‑CAGCCTCAATGCGCCAGGGCTTATGT  >  NC_000913/300148‑300326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`