Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,729,373 +C intergenic (‑195/+247) rrsG ← / ← clpB 16S ribosomal RNA/ClpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,729,3711.C99.2% 360.1 / ‑3.6 118intergenic (‑193/+249)rrsG/clpB16S ribosomal RNA/ClpB
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (36/81);  total (37/81)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 121 total aligned reads displayed.

CGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTT  >  NC_000913/2729282‑2729458
                                                                                           |                                                                                       
cGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TT‑CCg                                                                                      >  1:1521080/1‑93 (MQ=31)
                          aaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                             <  2:2727631/93‑1 (MQ=0)
                           aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                            >  2:4139277/1‑93 (MQ=9)
                           aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                            <  1:4370491/93‑1 (MQ=12)
                            ttGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                                                           >  1:1901251/1‑93 (MQ=12)
                             tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                          <  2:4270157/93‑1 (MQ=255)
                             tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                          <  2:2861503/93‑1 (MQ=16)
                              tttAAAGAGCAGTGCCGCTTCGCTTTTTCCCAGCGGCGCGGGGTGGGCATAATACGCC‑TTCCCGCCACAGAGTCAAGCATTTCTTTGCTTTtc                                                         <  1:3194207/92‑1 (MQ=11)
                              gTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                                         <  1:3194208/93‑1 (MQ=255)
                                tAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                                       <  1:603069/93‑1 (MQ=255)
                                tAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                                       <  1:4590329/93‑1 (MQ=255)
                                tAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                                       <  1:4124799/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  2:1014691/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  2:2213957/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  1:4175561/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  1:4175560/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  1:1202476/93‑1 (MQ=255)
                                 aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                      <  1:2548500/93‑1 (MQ=255)
                                  aaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                     <  2:2872394/93‑1 (MQ=255)
                                  aaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                     <  1:3291132/93‑1 (MQ=255)
                                    gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   <  1:755596/93‑1 (MQ=255)
                                    gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   <  1:2075387/93‑1 (MQ=255)
                                    gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   <  1:3877838/93‑1 (MQ=255)
                                    gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                   <  1:3077345/93‑1 (MQ=255)
                                     agCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                                  <  1:1449503/93‑1 (MQ=255)
                                     agCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                                  <  1:3272824/93‑1 (MQ=255)
                                     agCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                                  <  2:1514735/93‑1 (MQ=255)
                                     agCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                                  <  1:1094222/93‑1 (MQ=255)
                                      gCAGTGCCGCTTTGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCGTC‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 <  1:3951805/93‑1 (MQ=14)
                                      gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                                 <  1:4693090/93‑1 (MQ=255)
                                       cAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                                                <  2:244277/93‑1 (MQ=255)
                                        aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                               <  2:2518291/93‑1 (MQ=255)
                                        aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                               <  2:1391783/93‑1 (MQ=255)
                                         gTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                                              >  1:4517321/1‑93 (MQ=255)
                                           gCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc                                            >  2:3171532/1‑93 (MQ=255)
                                           gCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc                                            <  2:3315040/93‑1 (MQ=255)
                                             ctcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTGTCTCTGTTGAGATtctc                                          <  1:687537/91‑1 (MQ=17)
                                             cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          <  2:3789422/93‑1 (MQ=255)
                                             cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          <  1:2537174/93‑1 (MQ=255)
                                             cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                          <  1:4508802/93‑1 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         <  1:651447/93‑1 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:1367362/1‑93 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:1494540/1‑93 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:373197/1‑93 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:373195/1‑93 (MQ=255)
                                              gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         >  1:3434930/1‑93 (MQ=255)
                                              gcttcgcttGTTCTCAGCGGCGCGGCGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGAATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                         <  1:859129/93‑1 (MQ=17)
                                               cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                        <  2:1166818/93‑1 (MQ=255)
                                               cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                        >  1:816769/1‑93 (MQ=255)
                                               cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                        <  2:3735181/93‑1 (MQ=255)
                                               cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTCTTCTCTGTTGAGATTCTCAg                                        <  2:1679356/93‑1 (MQ=255)
                                                 tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                      <  2:3222759/93‑1 (MQ=255)
                                                 tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                      <  2:2663795/93‑1 (MQ=255)
                                                 tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                      >  1:2559254/1‑93 (MQ=255)
                                                  cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                     >  1:2065330/1‑93 (MQ=255)
                                                  cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                     >  1:2065331/1‑93 (MQ=255)
                                                   gcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                    >  1:2883274/1‑93 (MQ=255)
                                                    cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                   >  2:3036766/1‑93 (MQ=255)
                                                    cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                   <  2:4264842/93‑1 (MQ=255)
                                                     tttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc                                  <  2:4626319/93‑1 (MQ=255)
                                                      ttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                                 <  1:2939951/93‑1 (MQ=255)
                                                       tttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                <  1:1350854/93‑1 (MQ=255)
                                                       tttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                <  1:204663/93‑1 (MQ=255)
                                                       tttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                                <  1:3936036/93‑1 (MQ=255)
                                                       tttCCCAGCGGCGCGGATTATGCATAATACGCC‑TTACCGCTACAGAGTAAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAcccc                                <  2:1729081/93‑1 (MQ=255)
                                                           tcAGCGGCGCTGGGTGTGCATAATACGCC‑TTCCCGCTACAGCGTCAATCATTTCTTTTCTTTTCTCTGTTGAGATTCTCAGGAGAACCccacc                            <  1:1212811/93‑1 (MQ=17)
                                                           tcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                            >  2:3470690/1‑93 (MQ=255)
                                                           tcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                            >  2:2033498/1‑93 (MQ=255)
                                                           tcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                            <  1:3438532/93‑1 (MQ=255)
                                                           tcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                            >  1:4678277/1‑93 (MQ=255)
                                                            cAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgccg                           >  1:2746713/1‑93 (MQ=255)
                                                             aGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAATCCCGCCGa                          <  1:3023144/93‑1 (MQ=21)
                                                             aGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa                          >  1:809855/1‑93 (MQ=255)
                                                              gcggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                         >  2:3520251/1‑93 (MQ=255)
                                                               cggcgCGGGGTGTGCATAATACGNC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                        <  1:2003792/93‑1 (MQ=255)
                                                               cggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                        <  1:2730852/93‑1 (MQ=255)
                                                               cggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                        <  1:73779/93‑1 (MQ=255)
                                                                 gcgcGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcg                      <  1:3578420/93‑1 (MQ=255)
                                                                 gcgcGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcg                      >  2:4649207/1‑93 (MQ=255)
                                                                     ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                  <  1:890347/93‑1 (MQ=255)
                                                                     ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                  >  2:3904799/1‑93 (MQ=255)
                                                                     ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                  >  2:3904798/1‑93 (MQ=255)
                                                                      gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  1:3571856/1‑93 (MQ=255)
                                                                      gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  2:4064348/1‑93 (MQ=255)
                                                                      gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  1:4738762/1‑93 (MQ=255)
                                                                      gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGATTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc                 >  2:3294956/1‑93 (MQ=255)
                                                                        gtgtGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgt               <  2:3290629/93‑1 (MQ=255)
                                                                         tgtgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtg              >  1:1748606/1‑93 (MQ=255)
                                                                          gtgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtgt             <  1:789450/93‑1 (MQ=255)
                                                                            gCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt           <  2:3153897/93‑1 (MQ=255)
                                                                              ataataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGc         <  2:2901208/93‑1 (MQ=255)
                                                                               taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCCGGAGAACCCCGCCGACCCGGCGGCGGGGTTGcc        >  2:1953518/1‑93 (MQ=21)
                                                                               taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGACCCCCGCCGACCCGGCGGCGTGTTTGcc        <  1:543411/93‑1 (MQ=255)
                                                                               taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGcc        <  2:246011/93‑1 (MQ=255)
                                                                                 ataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgt      >  1:2906657/1‑93 (MQ=255)
                                                                                 ataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgt      <  1:3608786/93‑1 (MQ=255)
                                                                                 ataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgt      <  2:610897/93‑1 (MQ=255)
                                                                                  tccGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgtt     <  2:4243830/91‑1 (MQ=255)
                                                                                    cGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgttgt   <  2:4152045/93‑1 (MQ=255)
                                                                                     gCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCgttgtt  >  2:4393322/1‑93 (MQ=255)
                                                                                           |                                                                                       
CGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTT  >  NC_000913/2729282‑2729458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑