BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,585	+G	coding (953/2286 nt)	glcF ←	glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,582	1	.	G	100.0%	374.3 / NA	116	R319P (CGC→CCC)	glcF	glycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (64/52); total (64/52)

Only 100 of 135 total aligned reads displayed.

AGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123490‑3123669

aGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGc                                                                                            >  2:954528/1‑93 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                          <  1:2678123/93‑1 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                          >  1:2067942/1‑93 (MQ=255)

  ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg                                                                                         <  1:1617918/93‑1 (MQ=255)

   tCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg‑g                                                                                       <  1:2321180/93‑1 (MQ=255)

   tCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg‑g                                                                                       >  1:3251267/1‑93 (MQ=255)

     cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg‑ggc                                                                                     <  1:2452793/93‑3 (MQ=255)

     cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg‑ggc                                                                                     >  2:2199399/1‑91 (MQ=255)

     cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg‑ggc                                                                                     >  2:1505962/1‑91 (MQ=255)

          aCATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGc                                                                                <  1:2657198/93‑1 (MQ=255)

          aCATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGc                                                                                >  2:1419478/1‑93 (MQ=255)

           cATGCGACCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGcc                                                                               <  2:2568578/93‑1 (MQ=255)

            aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAATGAGCCGATAGCGGG‑G‑CAAAGcca                                                                              >  2:3245917/1‑93 (MQ=255)

            aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGaca                                                                              >  2:2429559/1‑93 (MQ=255)

             tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGccac                                                                             >  1:1231950/1‑93 (MQ=255)

               cGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGccacca                                                                           <  1:1031825/93‑1 (MQ=255)

                 aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCAtc                                                                         >  2:3000341/1‑93 (MQ=255)

                     gggTGAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCg                                                                     <  2:59219/93‑1 (MQ=255)

                      gggtAAACACGCCGCAAGGGTCGAGCTCCTGTTTAAGCTGGTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCgg                                                                    <  2:2505242/90‑1 (MQ=255)

                         tAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCAc                                                                 <  1:573319/93‑1 (MQ=255)

                            acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTaa                                                              <  2:4350889/93‑1 (MQ=255)

                             cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTaaa                                                             >  2:1115021/1‑93 (MQ=255)

                              acGCCGCAAGGTGCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAg                                                            <  2:3666879/93‑1 (MQ=255)

                               cgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGc                                                           >  1:2089537/1‑93 (MQ=255)

                               cgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGc                                                           >  2:3909141/1‑93 (MQ=255)

                               cgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGc                                                           <  1:3747311/93‑1 (MQ=255)

                                gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CACAGCCAACATCTCCGGCACTAAAGCg                                                          >  2:3205053/1‑93 (MQ=255)

                                gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCg                                                          <  2:2824314/93‑1 (MQ=255)

                                 ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCgg                                                         <  1:542084/93‑1 (MQ=255)

                                 ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCgg                                                         <  2:1186525/93‑1 (MQ=255)

                                  cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCggg                                                        <  1:66181/93‑1 (MQ=255)

                                  cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCggg                                                        <  2:4373819/93‑1 (MQ=255)

                                    cAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTc                                                      >  1:3197121/1‑93 (MQ=255)

                                     aaGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCg                                                     <  2:3378188/93‑1 (MQ=255)

                                      aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGc                                                    <  2:4117133/93‑1 (MQ=255)

                                       gggTCGAGCTGCTGTTTAGGCTGCTGTTGATAGCGGATTAAAGGAGCCGATAGCGGG‑G‑CAAGGCCACCATCTCCGGCACTAAAGCGGGTCGCa                                                   <  1:2124015/93‑1 (MQ=255)

                                        ggTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt                                                  >  1:3982855/1‑93 (MQ=255)

                                         gTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATg                                                 <  1:334027/93‑1 (MQ=255)

                                          tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGTCACCATCTCCGGCACTAAAGCGGGACGCATGa                                                >  1:913607/1‑93 (MQ=255)

                                          tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGa                                                <  1:1899025/93‑1 (MQ=255)

                                           cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc                                               <  2:2221163/93‑1 (MQ=255)

                                            gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                              >  1:18929/1‑93 (MQ=255)

                                             aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTTCGGCACTAAAGCGGGTTGCATGTCCg                                             >  2:4634643/1‑93 (MQ=255)

                                             aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg                                             >  2:3783607/1‑93 (MQ=255)

                                              gctgctGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc                                            <  2:1713510/93‑1 (MQ=255)

                                                tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  2:2048169/1‑93 (MQ=255)

                                                tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          <  1:2561029/93‑1 (MQ=255)

                                                tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  2:4469348/1‑93 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         <  1:3404976/93‑1 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGCGCCGCATGACCGCCAg                                         >  2:2910048/1‑93 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGAAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         <  2:2050491/93‑1 (MQ=255)

                                                  ctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGc                                        >  1:4420564/1‑93 (MQ=255)

                                                  ctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGc                                        >  2:3912057/1‑93 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      <  1:4552212/93‑1 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      <  1:2803714/93‑1 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      <  2:4606454/93‑1 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      <  1:1033480/93‑1 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGCCGCATCACCGCCAGCGt                                      >  1:1410796/1‑93 (MQ=255)

                                                     tttAAGGTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                     <  2:1054847/93‑1 (MQ=255)

                                                     tttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                     <  2:1418442/93‑1 (MQ=255)

                                                     tttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                     >  1:1939509/1‑93 (MQ=255)

                                                        aaGATGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                  >  2:3979342/1‑93 (MQ=255)

                                                         aGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                 >  2:16501/1‑93 (MQ=255)

                                                         aGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATATCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                 >  2:4078185/1‑93 (MQ=255)

                                                         aGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATATCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                 >  2:4078184/1‑93 (MQ=255)

                                                            tgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcg                              >  2:882925/1‑93 (MQ=255)

                                                            tgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcg                              >  1:3112987/1‑93 (MQ=255)

                                                             gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                             >  1:2945808/1‑93 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           >  1:3569737/1‑93 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           >  2:3231322/1‑93 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGCCGCATGACCGCCAGCGTTGCGGgcgatg                           >  2:1807835/1‑93 (MQ=255)

                                                                ggTGATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                          <  2:2962163/93‑1 (MQ=255)

                                                                   gATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcgat                       <  1:2589391/93‑1 (MQ=255)

                                                                   gATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcgat                       <  2:3802118/93‑1 (MQ=255)

                                                                   gATAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcgat                       <  1:836832/93‑1 (MQ=255)

                                                                     tAGCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg                     <  2:2935439/93‑1 (MQ=255)

                                                                       gCGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAt                   <  1:659933/93‑1 (MQ=255)

                                                                        cGGAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAtt                  <  2:2067942/93‑1 (MQ=255)

                                                                         ggAATAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGAGATGCGATGGAttt                 >  2:4715303/1‑93 (MQ=255)

                                                                           aaTAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               >  2:4241755/1‑93 (MQ=255)

                                                                           aaTAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               <  2:996834/93‑1 (MQ=255)

                                                                           aaTAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               >  1:4273767/1‑93 (MQ=255)

                                                                            aTAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              >  2:3369342/1‑93 (MQ=255)

                                                                            aTAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              >  1:1533040/1‑93 (MQ=255)

                                                                             tAAAGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGCTTTGAtt             >  2:1474583/1‑93 (MQ=255)

                                                                                aGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          >  2:1476225/1‑93 (MQ=255)

                                                                                aGGAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          >  1:2738169/1‑93 (MQ=255)

                                                                                 ggAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCCTGACCGCCAGCGTTGTGGGCGATGCGATGGATTTGATTGTct         >  1:1524157/1‑92 (MQ=255)

                                                                                 ggAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTcc         >  1:1524158/1‑93 (MQ=255)

                                                                                 ggAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTcc         <  2:2472354/93‑1 (MQ=255)

                                                                                  gAGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt        >  1:4127620/1‑93 (MQ=255)

                                                                                   aGCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc       <  1:1641012/93‑1 (MQ=255)

                                                                                    gCCGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg      >  1:611510/1‑93 (MQ=255)

                                                                                     ccGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCgg     <  1:520627/93‑1 (MQ=255)

                                                                                     ccGATAGC‑GGCG‑CAAACCCACCAGCTCCGGCACTAAAGCGGGGCGCATGACCCCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCgg     <  1:520756/93‑1 (MQ=255)

                                                                                      cGATAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGc    >  2:1669711/1‑93 (MQ=255)

                                                                                      cGATAGC‑GG‑GTCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCTATGGATTTGATTGTCCTCGGc    <  2:2326822/93‑1 (MQ=255)

                                                                                       gATAGCGGG‑G‑CAAAGCCAACATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCt   >  2:180098/1‑93 (MQ=255)

                                                                                        aTAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  1:2437213/1‑93 (MQ=255)

                                                                                        aTAGCGGG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  <  1:4743822/93‑1 (MQ=255)

AGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GG‑G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123490‑3123669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`