BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,525	(C)_6→7	coding (402/630 nt)	yqiE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,519	1	.	C	91.1%	366.3 / 28.6	124	coding (408/630 nt)	yqiE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (6/5); new base C (59/54); total (65/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATC  >  NC_000913/3175427‑3175608

cgAATATCTTCGTTTTCATCAGCCAGCCCGTAAATCCCGCTTGCGGTCGTGGCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGTg                                                                                               <  2:3532503/93‑1 (MQ=255)

cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTg                                                                                               >  1:933825/1‑93 (MQ=255)

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c                                                                                            >  2:2596171/1‑93 (MQ=255)

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c                                                                                            >  1:2056078/1‑93 (MQ=255)

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c                                                                                            >  2:2401157/1‑93 (MQ=255)

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c                                                                                            >  1:893004/1‑93 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c‑c                                                                                          >  2:1518286/1‑93 (MQ=255)

   atatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c‑c‑c                                                                                        >  1:3944588/1‑93 (MQ=255)

     tttttCGTTTGCATCAGCCAGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c‑c‑ccc                                                                                      <  2:1336150/90‑1 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c‑c‑cccc                                                                                     >  1:1220783/1‑93 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑c‑c‑cccc                                                                                     >  1:1581635/1‑93 (MQ=255)

            ttttTACCAGCCAGCCCGTGAATCCCGTTTGCGGCCGTGGCTTCCGCTTCCCCCACCATAATTGACGACCGCTCACTGGTG‑‑C‑CACCCCGGGCt                                                                                <  2:4187755/93‑1 (MQ=255)

            ttttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCt                                                                                <  2:2135005/93‑1 (MQ=255)

             tttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCtt                                                                               >  2:673446/1‑93 (MQ=255)

             ttccatcaGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCtt                                                                               <  2:289360/90‑1 (MQ=255)

              ttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTg                                                                              >  2:1198424/1‑93 (MQ=255)

                catcaGCCAGACGGTGAATCCCGCTTGCGGTCGTGCCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGcc                                                                            <  1:3789776/93‑1 (MQ=255)

                 atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTGGCCCACCATAATTAACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCa                                                                           >  1:4392952/1‑93 (MQ=255)

                 atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCC‑C‑CCCCGGGCTTGcc                                                                            >  2:1334090/1‑93 (MQ=255)

                 atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCa                                                                           >  1:686821/1‑93 (MQ=255)

                   caGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAgg                                                                         >  1:1721632/1‑93 (MQ=255)

                    aGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGa                                                                        <  2:84430/93‑1 (MQ=255)

                     gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGaa                                                                       <  2:2217425/93‑1 (MQ=255)

                      ccAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGaaa                                                                      >  1:976150/1‑93 (MQ=255)

                       cAGCCCGTGAATACCCCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAAc                                                                     <  1:3472898/93‑1 (MQ=255)

                       cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAAc                                                                     >  1:2484657/1‑93 (MQ=255)

                        aGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACt                                                                    >  1:1621417/1‑93 (MQ=255)

                        aGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGCG‑‑CTC‑CCCCGGGCGTGCCAGGTAACt                                                                    >  1:1869164/1‑93 (MQ=255)

                        aGACCGTGAATACCGCTTGCGGTCGGGGCGTGCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACt                                                                    <  2:2679898/93‑1 (MQ=255)

                         gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACtt                                                                   >  2:1036060/1‑93 (MQ=255)

                         gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACtt                                                                   >  2:2780559/1‑93 (MQ=255)

                           cggTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTaa                                                                 <  2:3071486/91‑1 (MQ=255)

                             atGAATACCGAGTGCGGGCGGGGCTTCCAATGCGCCCGCCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAaca                                                               <  2:251010/92‑1 (MQ=255)

                              tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAacac                                                              >  1:3370713/1‑93 (MQ=255)

                                aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGACCCCTCACTCGTG‑‑CAC‑CCCCGGGCTTGCCAGGACACTTAACACCg                                                            >  1:2301924/1‑93 (MQ=255)

                                aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCTGTTG‑C‑‑C‑C‑CCCCCGGCGTGCCCGGAAACTTAACACCCGt                                                          >  1:519071/1‑93 (MQ=255)

                                aaTACCGCTGGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCg                                                            <  2:1890062/93‑1 (MQ=255)

                                  tcccGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGt                                                          <  2:330254/91‑1 (MQ=255)

                                  tACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGt                                                          >  2:3302242/1‑93 (MQ=255)

                                   aCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACAACGGtt                                                         >  1:4676507/1‑93 (MQ=255)

                                     cGCTTGCGGCCCGGGCCTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                       <  1:349627/93‑1 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                     >  1:106984/1‑93 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                     >  1:106983/1‑93 (MQ=255)

                                        ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                    <  1:4418186/93‑1 (MQ=255)

                                          gCGGCCGGGGCGCCCACTCCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCg                                                  <  2:3797190/93‑1 (MQ=255)

                                            ggTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGtt                                                <  1:749958/93‑1 (MQ=255)

                                              gcGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                              <  2:3882261/92‑1 (MQ=255)

                                                gTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAc                                            >  2:3740420/1‑93 (MQ=255)

                                                 tGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACt                                           <  1:4476797/93‑1 (MQ=255)

                                                  ggCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTa                                          <  1:4627500/93‑1 (MQ=255)

                                                   gCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAt                                         >  2:3372259/1‑93 (MQ=255)

                                                     gTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                       <  1:1172711/93‑1 (MQ=255)

                                                     gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                       >  2:2571516/1‑93 (MQ=255)

                                                     gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                       >  1:3117296/1‑93 (MQ=255)

                                                      tCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                      >  1:2137970/1‑93 (MQ=255)

                                                       ccACTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAgc                                     >  1:3959967/1‑92 (MQ=255)

                                                       ccACTTCCCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                     <  2:4470824/93‑1 (MQ=255)

                                                        cgcTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                    <  1:2517598/91‑1 (MQ=255)

                                                        cACTCCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                    <  2:4272363/93‑1 (MQ=255)

                                                         cctccGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                   <  2:4612980/89‑1 (MQ=255)

                                                         cctccGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                   <  1:2120761/89‑1 (MQ=255)

                                                         aCTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                   >  1:646784/1‑93 (MQ=255)

                                                          cTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTccc                                  <  1:2434494/93‑1 (MQ=255)

                                                          cTTCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTccc                                  >  2:1643317/1‑93 (MQ=255)

                                                            tCGCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGc                                >  1:4285099/1‑93 (MQ=255)

                                                            ccccccACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGc                                <  2:3051509/90‑1 (MQ=255)

                                                              gCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCct                              >  1:4466984/1‑93 (MQ=255)

                                                              gCCCACCATAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCct                              >  2:3355394/1‑93 (MQ=255)

                                                                ccaccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                            <  1:2190443/93‑1 (MQ=255)

                                                                ccaccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                            >  1:1894766/1‑93 (MQ=255)

                                                                ccaccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                            <  2:1067369/93‑1 (MQ=255)

                                                                ccaccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                            >  1:3594364/1‑93 (MQ=255)

                                                                 caccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                           >  2:872770/1‑93 (MQ=255)

                                                                 caccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                           >  1:4084492/1‑93 (MQ=255)

                                                                  cccaAAATTGACGAACCCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTc                          <  2:4068255/92‑1 (MQ=255)

                                                                  accaTAATTGACGAACGCTCACTGGTGCCC‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                           >  1:1735672/1‑93 (MQ=255)

                                                                   ccaTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCa                         >  2:4585727/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                        >  2:40186/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                        <  1:3885496/93‑1 (MQ=255)

                                                                     aTAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAt                       <  1:126563/93‑1 (MQ=255)

                                                                      tAATTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATc                      <  1:4052415/93‑1 (MQ=255)

                                                                      tAATTGACGAACGCTCACTGGTG‑‑C‑C‑CCCCGGGCTTGCCACGAAACTTAACAGCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCg                     >  1:1994641/1‑93 (MQ=255)

                                                                       aaTTGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCg                     >  2:1251952/1‑93 (MQ=255)

                                                                          tGACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                  <  1:3121341/93‑1 (MQ=255)

                                                                           gACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCATTCCCGCCTCTTCAATCGCTTc                 <  2:4671666/93‑1 (MQ=255)

                                                                           gACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc                 <  1:334057/93‑1 (MQ=255)

                                                                           gACGAACGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc                 <  2:648392/93‑1 (MQ=255)

                                                                           gACGAACCCGCATTGGTG‑‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTcg                <  2:4495971/93‑1 (MQ=255)

                                                                             cGAACGCCCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc               <  2:451826/93‑1 (MQ=255)

                                                                               aaCGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa             >  1:2173373/1‑93 (MQ=255)

                                                                               aaCGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa             >  1:2048026/1‑93 (MQ=255)

                                                                                aCGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAc            >  2:137252/1‑93 (MQ=255)

                                                                                 cGCTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg           >  1:2979995/1‑93 (MQ=255)

                                                                                  gcgcACTGGTGCCC‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg           <  2:1435237/90‑1 (MQ=255)

                                                                                   cTCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg         <  1:4559097/93‑1 (MQ=255)

                                                                                    tCACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc        >  1:3031573/1‑93 (MQ=255)

                                                                                    tCACTGGGGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTACCACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc        <  1:769756/93‑1 (MQ=255)

                                                                                     cACTGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCa       >  1:2273503/1‑93 (MQ=255)

                                                                                     cACTGGTGC‑C‑C‑CCCCGGGCTGGCCAGCAACCTTAACACCGGTTTGCGCCGTTGGACTACCAGGCCCGCCTCTTCAATCGCTTCGCGACGGGCa       <  2:2386781/93‑1 (MQ=255)

                                                                                        tGGTGC‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa    <  2:3228223/93‑1 (MQ=255)

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑‑C‑C‑CCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATC  >  NC_000913/3175427‑3175608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`