BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,181	(T)_5→6	intergenic (+200/‑22)	ygiL → / → yi215	putative fimbrial‑like protein/IS2 hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,176	1	.	T	100.0%	113.7 / NA	37	intergenic (+195/‑27)	ygiL/yi215	putative fimbrial‑like protein/IS2 hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (20/17); total (20/17)

AAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184085‑3184201

aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  1:3820260/93‑1 (MQ=255)

 aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         >  1:570232/1‑93 (MQ=255)

  atatTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttt                        >  2:1600389/1‑93 (MQ=255)

   tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:3438089/1‑93 (MQ=255)

   tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  1:4276779/1‑93 (MQ=255)

   tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:254515/1‑93 (MQ=255)

   tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       <  2:2503379/93‑1 (MQ=255)

   tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:1608367/1‑93 (MQ=255)

    atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGCCACCAAATATACCTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      >  2:3723154/1‑93 (MQ=18)

    atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  2:34865/93‑1 (MQ=255)

    atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  1:1380605/93‑1 (MQ=255)

     tttattGATCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:1933791/1‑92 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    >  1:482172/1‑91 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:3625368/93‑3 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:3625369/93‑3 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:3870169/93‑3 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:4276701/93‑3 (MQ=255)

      ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  2:3160452/93‑3 (MQ=255)

       tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   >  1:778451/1‑90 (MQ=255)

       tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   <  1:1409194/93‑4 (MQ=255)

       tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   >  1:3787614/1‑90 (MQ=255)

        attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  2:340797/1‑89 (MQ=255)

        attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  <  1:2453108/93‑5 (MQ=255)

          tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:3550872/93‑1 (MQ=255)

          tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 >  1:3223475/1‑93 (MQ=255)

          tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:2247095/93‑1 (MQ=255)

          tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:1930611/93‑1 (MQ=255)

           gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACtg                >  1:3953361/1‑92 (MQ=255)

           gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                <  2:2050608/93‑1 (MQ=255)

           gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  2:1518492/1‑93 (MQ=255)

            ttaaTTATCAGTTATTAGACTGGCCCACTGAATCTACAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:479388/4‑93 (MQ=14)

             tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  2:864540/1‑93 (MQ=255)

              cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             <  2:1292293/93‑1 (MQ=255)

              cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             <  1:4509550/93‑1 (MQ=255)

               aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  2:1752667/1‑93 (MQ=255)

                ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:3893076/1‑93 (MQ=255)

                ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           <  1:280899/93‑1 (MQ=255)

                ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  1:294879/1‑93 (MQ=255)

                ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           <  2:2125346/93‑1 (MQ=255)

                 tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  2:511457/1‑93 (MQ=255)

                 tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:2398456/93‑1 (MQ=255)

                 tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:512767/93‑1 (MQ=255)

                 tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:3398851/93‑1 (MQ=255)

                 tATCCGTTATTAGACTGGCACCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:512768/93‑1 (MQ=255)

                  aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  2:3014926/93‑1 (MQ=255)

                   tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:935919/1‑93 (MQ=255)

                   tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  1:823415/93‑1 (MQ=255)

                   tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  1:823416/93‑1 (MQ=255)

                    ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTGGTCATTGga       <  1:4063417/93‑1 (MQ=255)

                    ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  1:506007/1‑93 (MQ=255)

                    ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  2:636706/1‑93 (MQ=255)

                    ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  1:256504/1‑93 (MQ=255)

                      gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  1:2106903/1‑93 (MQ=255)

                       ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  1:3522980/1‑93 (MQ=16)

                       ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    <  1:766623/93‑1 (MQ=16)

                       ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:1886094/1‑93 (MQ=16)

                        tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgagagc   >  1:3683668/1‑91 (MQ=255)

                         attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:4733956/1‑93 (MQ=9)

                         attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:468237/1‑93 (MQ=9)

                         attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:4297008/1‑93 (MQ=9)

AAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184085‑3184201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`