BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,926	+C	coding (1052/1434 nt)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,924	1	.	C	99.3%	461.5 / ‑3.9	143	coding (1050/1434 nt)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (74/68); total (75/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 150 total aligned reads displayed.

TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224832‑3225011

tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                         <  2:4288902/93‑1 (MQ=255)

  tGGTAAGAAAGCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c‑cc                                                                                      <  1:21717/93‑2 (MQ=255)

  tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c‑cc                                                                                      <  1:21716/93‑2 (MQ=255)

    gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c‑ccga                                                                                    <  2:4633173/93‑4 (MQ=255)

       aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGc                                                                                  <  1:590239/93‑1 (MQ=255)

        gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCt                                                                                 >  2:4437963/1‑93 (MQ=255)

          aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCg                                                                               <  1:710151/93‑1 (MQ=255)

          aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CCATGCTCg                                                                               >  1:1755721/1‑93 (MQ=255)

             ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGtt                                                                            >  2:761065/1‑93 (MQ=255)

             ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGtt                                                                            <  1:3234768/93‑1 (MQ=255)

             ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGtt                                                                            >  1:3800612/1‑93 (MQ=255)

              cGTCCAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTc                                                                           <  2:1057470/93‑1 (MQ=255)

                 cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCaa                                                                        <  1:460353/93‑1 (MQ=255)

                 cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCaa                                                                        <  1:2500840/93‑1 (MQ=255)

                  gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAAt                                                                       <  2:479426/93‑1 (MQ=255)

                   aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATa                                                                      >  2:2531114/1‑93 (MQ=255)

                   aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAGCGTCATCCCGCTGATGACGATCC‑CGATGCTCGGTTCCAATa                                                                      >  1:4221621/1‑93 (MQ=255)

                    aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAc                                                                     <  2:2083714/93‑1 (MQ=255)

                    aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAc                                                                     >  2:934692/1‑93 (MQ=255)

                    aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAc                                                                     >  1:2641001/1‑93 (MQ=255)

                     cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAcc                                                                    <  1:4329146/93‑1 (MQ=255)

                      tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCg                                                                   >  1:1982752/1‑93 (MQ=255)

                        aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTg                                                                 <  1:1447745/93‑1 (MQ=255)

                         aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGc                                                                <  1:4265132/93‑1 (MQ=255)

                          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCa                                                               <  1:1064985/93‑1 (MQ=255)

                          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCa                                                               <  1:1748429/93‑1 (MQ=255)

                          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCa                                                               <  2:1568249/93‑1 (MQ=255)

                          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCa                                                               >  1:1664293/1‑93 (MQ=255)

                          cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCa                                                               <  2:67428/93‑1 (MQ=255)

                           gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAg                                                              <  2:2276489/93‑1 (MQ=255)

                           gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAg                                                              >  1:434551/1‑93 (MQ=255)

                             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGa                                                            >  1:740434/1‑93 (MQ=255)

                             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGa                                                            <  2:1236331/93‑1 (MQ=255)

                             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGa                                                            >  1:4041357/1‑93 (MQ=255)

                             aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGa                                                            >  1:2652738/1‑93 (MQ=255)

                              aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGAt                                                           >  2:295142/1‑93 (MQ=255)

                               aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  1:663771/93‑1 (MQ=255)

                               aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  1:1841045/93‑1 (MQ=255)

                               aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATc                                                          >  2:4395750/1‑93 (MQ=255)

                                cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCt                                                         >  2:3365101/1‑93 (MQ=255)

                                cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCt                                                         <  1:2817741/93‑1 (MQ=255)

                                 ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                        >  2:1581556/1‑93 (MQ=255)

                                 ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                        <  1:2251032/93‑1 (MQ=255)

                                  gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CACGATGCTCGGTTCCAATATCGTGCAGGATCtga                                                       >  1:4404751/1‑93 (MQ=255)

                                     ttCCGGAGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGCGCAGGATCtgatga                                                    >  2:1701179/1‑93 (MQ=255)

                                        cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 >  1:3146945/1‑93 (MQ=255)

                                        cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 >  2:4121741/1‑93 (MQ=255)

                                            cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                              <  2:1442511/90‑1 (MQ=255)

                                             gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                                             >  1:1441742/3‑93 (MQ=255)

                                             gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                                             <  2:3263085/91‑1 (MQ=255)

                                              cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                            <  1:1911782/92‑1 (MQ=255)

                                               gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           >  2:2537097/1‑93 (MQ=255)

                                               gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           <  2:4319626/93‑1 (MQ=255)

                                               gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           <  1:352923/93‑1 (MQ=255)

                                                cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                          >  2:1745214/1‑93 (MQ=255)

                                                  cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        >  2:3208758/1‑93 (MQ=255)

                                                  cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        >  1:1775967/1‑93 (MQ=255)

                                                  cAGCGTGGATCCAGTGCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        <  2:130194/93‑1 (MQ=255)

                                                   aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       >  2:2769473/1‑93 (MQ=255)

                                                   aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       >  1:2834734/1‑93 (MQ=255)

                                                   aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       >  2:1109352/1‑93 (MQ=255)

                                                    gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      >  2:202375/1‑93 (MQ=255)

                                                     cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  2:126642/93‑1 (MQ=255)

                                                      gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  2:4037148/93‑1 (MQ=255)

                                                        ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                  >  1:777599/1‑93 (MQ=255)

                                                         gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 <  1:1807670/93‑1 (MQ=255)

                                                         gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 >  2:1453581/1‑93 (MQ=255)

                                                           tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               >  2:3227194/1‑93 (MQ=255)

                                                           tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               >  2:3227195/1‑93 (MQ=255)

                                                            ccAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgc                              >  1:1897896/1‑93 (MQ=255)

                                                             cAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                             >  2:2352106/1‑93 (MQ=255)

                                                               gTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc                           <  1:4270032/93‑1 (MQ=255)

                                                                ttCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          >  1:3384542/1‑93 (MQ=255)

                                                                ttCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          >  2:615451/1‑93 (MQ=255)

                                                                  ccTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGc                        >  1:3581213/1‑93 (MQ=255)

                                                                   cTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCg                       >  1:420199/1‑93 (MQ=255)

                                                                    tGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGt                      >  1:3629568/1‑93 (MQ=255)

                                                                     gATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     <  1:3975136/93‑1 (MQ=255)

                                                                      aTCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc                    >  2:2972472/1‑93 (MQ=255)

                                                                       tCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   <  1:1767093/93‑1 (MQ=255)

                                                                       tCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   <  1:4687713/93‑1 (MQ=255)

                                                                       tCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   <  1:920580/93‑1 (MQ=255)

                                                                         gTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATg                 >  2:1534036/1‑93 (MQ=255)

                                                                          tCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGc                >  1:3406715/1‑93 (MQ=255)

                                                                           cATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt               <  1:712730/93‑1 (MQ=255)

                                                                           cATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt               <  1:4433524/93‑1 (MQ=255)

                                                                            aTCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt              <  1:1891737/93‑1 (MQ=255)

                                                                             tCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             >  2:4403273/1‑93 (MQ=255)

                                                                             tCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             >  1:1049328/1‑93 (MQ=255)

                                                                              cccGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTcc            <  2:2790772/93‑1 (MQ=255)

                                                                              cccGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTcc            <  1:4704968/93‑1 (MQ=255)

                                                                                 gCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcg         >  1:1689524/1‑92 (MQ=255)

                                                                                 gCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc         >  2:3702019/1‑93 (MQ=255)

                                                                                 gCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc         <  2:1461974/93‑1 (MQ=255)

                                                                                   tgatgaTTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGt       >  2:4453638/1‑93 (MQ=255)

                                                                                    gatgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  1:3158167/1‑93 (MQ=255)

                                                                                      tgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    <  1:2907459/93‑1 (MQ=255)

                                                                                      tgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    >  1:2583432/1‑93 (MQ=255)

                                                                                       gatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAGGACCGCCGCAGCGTCCATGCTTCCGCCGTTAtt   <  1:4294542/93‑1 (MQ=255)

                                                                                        atTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATtc  <  1:2277110/93‑1 (MQ=255)

TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224832‑3225011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`