Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,237,247 +C intergenic (+60/‑337) ygjT → / → ygjU putative transport protein/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,237,2451.C100.0% 334.9 / NA 106intergenic (+58/‑339)ygjT/ygjUputative transport protein/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (84/22);  total (84/22)

Only 100 of 112 total aligned reads displayed.

TATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCT‑C‑CTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCAAACGGG  >  NC_000913/3237158‑3237331
                                                                                          |                                                                                       
tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTctcc‑ctct‑ccct                                                                                     <  2:1247106/93‑3 (MQ=255)
    ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCa                                                                                  <  1:2696623/93‑1 (MQ=255)
     gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAg                                                                                 >  1:945656/1‑93 (MQ=255)
      cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAgg                                                                                <  2:1713495/93‑1 (MQ=255)
       aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAggg                                                                               >  2:3369668/1‑93 (MQ=255)
        tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGga                                                                              >  1:4634464/1‑93 (MQ=255)
        tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGga                                                                              >  1:433805/1‑93 (MQ=255)
         gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgag                                                                             >  1:398001/1‑93 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgaga                                                                            <  2:1980432/93‑1 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgaga                                                                            >  1:2589376/1‑93 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgaga                                                                            <  2:218676/93‑1 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgaga                                                                            >  2:3769212/1‑93 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGgaga                                                                            <  2:858710/93‑1 (MQ=255)
          aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCAGTGTCCCTTGTCATTAACATGAGCACTGTTTTCT‑CGCTCTCCCTTCCAGGgaga                                                                            <  2:2879408/93‑1 (MQ=255)
           tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGagag                                                                           >  1:3590699/1‑93 (MQ=255)
            aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAgg                                                                          >  1:3964500/1‑93 (MQ=255)
            aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAgg                                                                          >  2:1720809/1‑93 (MQ=255)
            aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAgg                                                                          >  2:1737577/1‑93 (MQ=255)
             agcagcGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAggg                                                                         <  1:4590460/93‑1 (MQ=255)
              gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGt                                                                        <  2:2102457/93‑1 (MQ=255)
              acagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGt                                                                        <  1:2479151/92‑1 (MQ=255)
               cagcGGGGTTGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTc                                                                       <  1:1593871/93‑1 (MQ=255)
                agcGGGGTGGATAATTTTTAATCTGCTTAAGCCGTGTCCCCTGTAATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTCCCAGGGAGAGGGTCg                                                                      <  2:3200274/93‑1 (MQ=255)
                 gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgg                                                                     <  1:259848/93‑1 (MQ=255)
                 gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgg                                                                     >  1:3961212/1‑93 (MQ=255)
                 gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCATGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgg                                                                     <  2:3049855/93‑1 (MQ=255)
                  cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCggg                                                                    >  2:3604457/1‑93 (MQ=255)
                   ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgggg                                                                   >  1:4721048/1‑93 (MQ=255)
                   ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgggg                                                                   >  2:3286862/1‑93 (MQ=255)
                   ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgggg                                                                   >  1:1743529/1‑93 (MQ=255)
                   ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCgggg                                                                   >  2:1150850/1‑93 (MQ=255)
                    gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                  <  2:2830424/93‑1 (MQ=255)
                    gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                  <  1:1416393/93‑1 (MQ=255)
                    gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                  >  1:3609969/1‑93 (MQ=255)
                     ggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTg                                                                 >  2:1791271/1‑93 (MQ=255)
                      gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTgg                                                                >  1:1175132/1‑92 (MQ=255)
                      gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                >  2:1870028/1‑93 (MQ=255)
                      gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                >  2:1870027/1‑93 (MQ=255)
                        ggATAATTTTTAATCTGCCTAAGCTGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                              >  2:293138/1‑93 (MQ=38)
                        ggATAATTTTTAATCTGCCTAAGCCGTGTCCTCTGTCATTAACAGGAGCTCCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGGGAgg                                                              <  1:434105/93‑1 (MQ=255)
                        ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                              >  2:2741843/1‑93 (MQ=39)
                        ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                              <  1:3151534/93‑1 (MQ=255)
                        ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                              >  2:633703/1‑93 (MQ=39)
                         gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCACTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                             >  1:86581/1‑93 (MQ=38)
                           tAATTTTTAATCTGCCTAAGCCGTGTCCCCTGTCATTAACATGAGAACTGTTTTCT‑CACTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                           >  2:3053686/1‑93 (MQ=255)
                           tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                           >  2:4008538/1‑93 (MQ=38)
                             aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                         >  2:2889382/1‑93 (MQ=38)
                             aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                         >  1:3256862/1‑93 (MQ=38)
                              tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                        >  1:71973/1‑93 (MQ=38)
                              tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                        >  1:1354721/1‑93 (MQ=38)
                                tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                      >  2:98389/1‑93 (MQ=38)
                                  tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGGAATTTTTc                                                    >  2:1719192/1‑93 (MQ=38)
                                  tAATCTGCCTAAGCCGTGTACCCTGTCATTAACACGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                    >  2:1064857/1‑93 (MQ=38)
                                    aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGc                                                  >  2:3929838/1‑93 (MQ=38)
                                      cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                >  2:1320704/1‑93 (MQ=38)
                                       tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  1:3954487/1‑93 (MQ=38)
                                       tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  1:337567/1‑93 (MQ=38)
                                       tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  1:2135084/1‑93 (MQ=38)
                                       tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAAGGTAATTTTTCGCAcc                                               >  2:1801816/1‑93 (MQ=38)
                                       tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCAcc                                               >  2:1154310/1‑93 (MQ=38)
                                        gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                              >  2:1866931/1‑93 (MQ=38)
                                         ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                             >  2:4177778/1‑93 (MQ=38)
                                          cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                            >  2:4658097/1‑93 (MQ=38)
                                           tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  2:962645/1‑93 (MQ=38)
                                           tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  2:1187056/1‑93 (MQ=38)
                                           tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  1:283194/1‑93 (MQ=38)
                                            aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCGAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGc                                          >  1:4403691/1‑93 (MQ=38)
                                            aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGc                                          >  2:856789/1‑93 (MQ=37)
                                            aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGc                                          >  1:475358/1‑93 (MQ=38)
                                               ccGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTgg                                       >  2:144991/1‑93 (MQ=37)
                                                 gtttACCCTGTCATTAACATGAGCCCCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGCTGAGGGTAATTTTTCGCACCGATGCTGGcc                                     <  1:1899231/90‑1 (MQ=37)
                                                 gtgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                     >  1:1450786/1‑93 (MQ=37)
                                                  tgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  1:3695587/1‑93 (MQ=37)
                                                  tgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  1:16661/1‑93 (MQ=37)
                                                  tgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  2:160641/1‑93 (MQ=37)
                                                  tgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  1:2026034/1‑93 (MQ=37)
                                                  tgtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  1:2415692/1‑93 (MQ=37)
                                                   gtACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                   >  1:25592/1‑93 (MQ=37)
                                                    tACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                  >  1:3481402/1‑93 (MQ=37)
                                                    tACCCTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGCGAGGGGAAGTTTTCGCACCGATGCTGGCCTgg                                  >  2:2601887/1‑92 (MQ=37)
                                                       ccTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑cc                              >  2:1663690/1‑93 (MQ=37)
                                                        cTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑tcc                             >  2:3644563/1‑93 (MQ=25)
                                                        cTGTCATTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑tcc                             >  2:4726721/1‑93 (MQ=37)
                                                             aTTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑cccccccc                        >  1:2883822/1‑89 (MQ=25)
                                                             aTTAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCAc                        >  1:593093/1‑93 (MQ=25)
                                                             aTTAACACGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCAc                        >  1:2468127/1‑93 (MQ=21)
                                                             aTTAACACGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCAc                        >  1:2468128/1‑93 (MQ=21)
                                                               tAACATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACTGATGCTGGCCTGTT‑CTCCTCAccc                      >  1:4261557/1‑93 (MQ=25)
                                                                aaCATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑TCccccccccc                     >  1:1915179/1‑92 (MQ=25)
                                                                 aCATGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGGAATTTTTCGCACCGATGCTGGCCTGTT‑TCCCCCAcccca                    >  2:2120579/1‑91 (MQ=25)
                                                                   aTGAGCACCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑cccccccccccctc                  >  2:377361/1‑89 (MQ=21)
                                                                         aCCGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑TCCCCCCCCCCCACCCcctc            >  2:1090720/1‑93 (MQ=21)
                                                                           cGTTTTCTCC‑CTCTCCCTTCCAGGGAGAGGGGCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCCTACCCTCTccc          >  1:248268/1‑93 (MQ=17)
                                                                             ttttCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCa        >  2:782188/1‑93 (MQ=17)
                                                                             ttttCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCa        >  1:2220668/1‑93 (MQ=18)
                                                                              tttCTCC‑CTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCaa       >  1:407509/1‑93 (MQ=17)
                                                                                 ctcc‑ctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTCACCCTCTCCCCCAACg    >  1:4539789/4‑93 (MQ=18)
                                                                                  tcc‑ctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCCCCCCCCCACCCCCCCCCCCAAACg    >  2:1258037/3‑93 (MQ=18)
                                                                                  tcc‑ctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCCCCCCCCAAACCCCCCCCCCAAcag   >  1:403182/3‑91 (MQ=18)
                                                                                   cc‑ctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCAAACggg  >  1:828375/2‑93 (MQ=255)
                                                                                          |                                                                                       
TATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCT‑C‑CTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT‑CCCCTCACCCTAACCCTCTCCCCAAACGGG  >  NC_000913/3237158‑3237331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑