BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,304	+C	coding (398/987 nt)	yrbH →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,301	1	.	C	99.0%	646.2 / ‑1.3	202	coding (395/987 nt)	yrbH	putative isomerase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (101/98); total (102/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 226 total aligned reads displayed.

TGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAC  >  NC_000913/3339209‑3339388

  cTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGATAATCTGCATCACCGGTC‑G‑cc                                                                                       >  2:478471/1‑93 (MQ=255)

    aTCTCTAACTCTTGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑cccc                                                                                     <  2:2938200/93‑2 (MQ=255)

    aTCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑cccc                                                                                     <  2:757569/93‑2 (MQ=255)

     tctcTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccg                                                                                    <  1:4236612/93‑3 (MQ=255)

     tctcTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccg                                                                                    >  1:3783650/1‑91 (MQ=255)

       tctAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑ccccgga                                                                                  <  1:462130/93‑5 (MQ=255)

           aCTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagc                                                                               >  2:1838762/1‑93 (MQ=255)

            ctctGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagca                                                                              >  2:3477643/1‑93 (MQ=255)

            ctctGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagca                                                                              >  2:1481758/1‑93 (MQ=255)

            ctctGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGagca                                                                              >  1:1439750/1‑93 (MQ=255)

               tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTCATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           >  2:4545004/1‑93 (MQ=255)

               tGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAgcagca                                                                           <  2:4664538/93‑1 (MQ=255)

                ggTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCAt                                                                          >  1:1947807/1‑93 (MQ=255)

                 gTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATg                                                                         <  1:438818/93‑1 (MQ=255)

                     aTCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgc                                                                     <  1:4027222/93‑1 (MQ=255)

                      tCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgcg                                                                    >  1:4466145/1‑93 (MQ=255)

                       ccAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgcgc                                                                   <  2:4438208/93‑1 (MQ=255)

                         aGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGgcgcgcgc                                                                 <  1:228540/93‑1 (MQ=255)

                          gCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgcc                                                                <  1:2479434/93‑1 (MQ=255)

                          gCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgcc                                                                >  1:621124/1‑93 (MQ=255)

                          gCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgcc                                                                >  1:1973048/1‑93 (MQ=255)

                          gCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGcgcc                                                                >  1:3264459/1‑93 (MQ=255)

                             aaaTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCa                                                             >  1:1688716/1‑93 (MQ=255)

                              aaTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAg                                                            >  1:939405/1‑93 (MQ=255)

                               aTCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGa                                                           <  1:1240095/93‑1 (MQ=255)

                                tCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAt                                                          <  2:2056447/93‑1 (MQ=255)

                                tCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGAt                                                          <  2:2094138/93‑1 (MQ=255)

                                  ccGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGGGAGCAGCATGGCGCGCGCCGCAGAtgt                                                        <  2:1240268/92‑1 (MQ=255)

                                     gCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCa                                                     <  1:2687737/93‑1 (MQ=255)

                                     gCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCa                                                     >  1:1199109/1‑93 (MQ=255)

                                      ccTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCGTCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCAt                                                    >  2:36724/1‑93 (MQ=255)

                                       cTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATc                                                   <  1:34307/93‑1 (MQ=255)

                                       cTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATc                                                   >  1:900789/1‑93 (MQ=255)

                                           aTTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtg                                               <  1:2519794/93‑1 (MQ=255)

                                           aTTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtg                                               <  1:2206290/93‑1 (MQ=255)

                                            ttCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgt                                              <  2:1851904/93‑1 (MQ=255)

                                             tCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgtg                                             >  1:281047/1‑93 (MQ=255)

                                             tCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgtg                                             >  2:2547397/1‑93 (MQ=255)

                                             tCCAGTGCTTAAGCGTCCTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCtgtgtg                                             <  1:2607852/93‑1 (MQ=255)

                                               cAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCGCCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGtt                                           <  2:3366048/93‑1 (MQ=255)

                                               cAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGtt                                           <  2:1167311/93‑1 (MQ=255)

                                               cAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGtt                                           >  1:4492477/1‑93 (MQ=255)

                                               cAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGAAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGtt                                           >  1:2527680/1‑93 (MQ=255)

                                                aGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTa                                          >  2:2715598/1‑93 (MQ=255)

                                                  tGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTaaa                                        >  1:1813340/1‑93 (MQ=255)

                                                  tGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTaaa                                        <  2:1007034/93‑1 (MQ=255)

                                                   gCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAg                                       >  1:2985178/1‑93 (MQ=255)

                                                    cTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGt                                      <  2:236163/93‑1 (MQ=255)

                                                    cTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGt                                      <  1:3964215/93‑1 (MQ=255)

                                                     ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     <  2:884767/93‑1 (MQ=255)

                                                     ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     >  2:2500232/1‑93 (MQ=255)

                                                     ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     <  2:835962/93‑1 (MQ=255)

                                                     ttAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTa                                     >  1:3451211/1‑93 (MQ=255)

                                                      tAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAg                                    <  2:614511/93‑1 (MQ=255)

                                                       aaGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGc                                   <  2:1631077/93‑1 (MQ=255)

                                                        aGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCg                                  >  2:280236/1‑93 (MQ=255)

                                                        aGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCg                                  >  1:2794641/1‑93 (MQ=255)

                                                        aGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCg                                  >  1:1776007/1‑93 (MQ=255)

                                                         gCGTCTTCACGTACCGTTAATCTGCATCACCGGTCTC‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGCAGCGa                                 >  1:847237/1‑93 (MQ=255)

                                                         gCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGa                                 <  2:1528065/93‑1 (MQ=255)

                                                         gCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGa                                 >  1:696839/1‑93 (MQ=255)

                                                         gCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGa                                 >  2:4128406/1‑93 (MQ=255)

                                                         gCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGa                                 >  1:3046652/1‑93 (MQ=255)

                                                           gTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGaaa                               >  1:881115/1‑93 (MQ=255)

                                                           gTCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGGTAAAGTAGCGaaa                               >  2:419855/1‑93 (MQ=255)

                                                            tCTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaag                              >  1:2630537/1‑93 (MQ=255)

                                                            tCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaaga                             <  2:4524379/93‑1 (MQ=255)

                                                             cTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaaga                             >  1:1181764/1‑93 (MQ=255)

                                                             cTTCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaaga                             >  1:3688754/1‑93 (MQ=255)

                                                              ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            >  2:1153046/1‑93 (MQ=255)

                                                              ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            <  2:3709383/93‑1 (MQ=255)

                                                              ttCACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAaagaa                            <  1:4298664/93‑1 (MQ=255)

                                                                cACGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGc                          >  1:4318698/1‑93 (MQ=255)

                                                                 aCGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGcc                         <  1:1118361/93‑1 (MQ=255)

                                                                  cGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCt                        <  1:1474379/93‑1 (MQ=255)

                                                                  cGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCt                        <  1:1662623/93‑1 (MQ=255)

                                                                  cGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCt                        <  2:3899514/93‑1 (MQ=255)

                                                                  cGTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCt                        >  2:3630185/1‑93 (MQ=255)

                                                                   gTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTg                       <  1:2142501/93‑1 (MQ=255)

                                                                   gTACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTg                       <  2:1884084/93‑1 (MQ=255)

                                                                    tACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGt                      <  2:2747279/93‑1 (MQ=255)

                                                                    tACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGt                      >  1:4341793/1‑93 (MQ=255)

                                                                    tACCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGt                      <  2:3581859/93‑1 (MQ=255)

                                                                     aCCGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTc                     <  1:2130144/93‑1 (MQ=255)

                                                                       cGTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCg                   >  1:4290550/1‑93 (MQ=255)

                                                                        gTTAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGt                  <  2:4120898/93‑1 (MQ=255)

                                                                         ttAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGtt                 >  2:3261286/1‑93 (MQ=255)

                                                                          tAATCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTa                <  2:3403291/93‑1 (MQ=255)

                                                                           aaTCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGACAGAAGCCTGTCCGTTAg               >  1:3587509/1‑93 (MQ=255)

                                                                             tCTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAggg             >  1:679821/1‑93 (MQ=255)

                                                                              cTGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCTCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGc            <  1:210145/93‑1 (MQ=255)

                                                                               tGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCt           >  2:833563/1‑93 (MQ=255)

                                                                               tGCATCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCt           <  1:3358865/93‑1 (MQ=255)

                                                                                  aTCACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGc        >  2:3172554/1‑93 (MQ=255)

                                                                                    cACCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAc      <  2:2176772/93‑1 (MQ=255)

                                                                                     aCCGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCAcc     >  2:3130125/1‑93 (MQ=255)

                                                                                      ccGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCg    >  2:3762181/1‑93 (MQ=255)

                                                                                       cGGTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGa   >  1:2832031/1‑93 (MQ=255)

                                                                                        ggTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAc  >  2:224569/1‑93 (MQ=255)

                                                                                        ggTC‑GCCCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAc  <  1:3387754/93‑1 (MQ=255)

TGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTC‑G‑CCCGGAGAGCAGCATGGCGCGCGCCGCAGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGAC  >  NC_000913/3339209‑3339388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`