BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	98.7%	500.0 / NA	156	V54V (GTC→GTG)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (64/90); minor base A (0/2); total (64/92)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑GGA‑CG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                       >  1:425909/1‑93 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                       <  2:4604009/93‑1 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                       <  2:2366748/93‑1 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                     <  1:1812870/93‑1 (MQ=255)

     cATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcgg                                                                                 <  1:4486401/93‑5 (MQ=255)

                   ccGTGGCGCTTGCTGTTGGATTAAGGCAAGGAATGCCGCTGGATAACGTGATTAGCTCCATCAAAGCCGGTGTCAGCGGGA‑CGCTCGGTAGcc                                                                   <  2:3536388/92‑1 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCt                                                                  <  1:3838179/93‑1 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCt                                                                  <  2:3165191/93‑1 (MQ=255)

                     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCtt                                                                 >  1:1277365/1‑93 (MQ=255)

                     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCtt                                                                 <  2:1063644/93‑1 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGccc                                                             <  2:1888565/93‑1 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGccc                                                             <  1:2363813/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCt                                                            <  2:4261886/93‑1 (MQ=255)

                             tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGAt                                                         <  2:4697983/93‑1 (MQ=255)

                             tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGAt                                                         >  2:2955420/1‑93 (MQ=255)

                              gccgTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATc                                                        <  1:1597927/90‑1 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATc                                                        <  1:4464944/93‑1 (MQ=255)

                               cTGTTGGATTCATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTGCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCa                                                       <  2:762057/93‑1 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCa                                                       <  1:1229933/93‑1 (MQ=255)

                                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:1965013/93‑1 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATg                                                     >  2:2786709/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATg                                                     >  2:1426498/1‑93 (MQ=255)

                                   tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATggg                                                   <  2:1446/93‑1 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  1:2587349/93‑1 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  1:3036900/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:2061471/1‑93 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               <  1:4667094/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  2:2123065/1‑93 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  1:2324345/93‑1 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  1:4362493/93‑1 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGgc                                            >  1:918434/1‑93 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATAAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  2:696056/1‑93 (MQ=255)

                                             cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:2691342/1‑93 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:3908447/1‑93 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  2:4056128/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  2:4052436/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:2410079/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:3662840/93‑1 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCATGATCATGGGTTTTGGCGCAAt                                       <  1:3747036/93‑1 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      <  2:1953898/93‑1 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  1:2729835/1‑93 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      <  1:1145982/93‑1 (MQ=255)

                                                  aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    >  1:2343130/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGTTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  2:3968893/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACCC‑TCGGTAGCCTTGCCCTGATCATGGGGTTTGGGGCAATGCTg                                   >  1:2750335/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:468356/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:2686486/1‑93 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  2:2964480/93‑1 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  2:3157782/93‑1 (MQ=255)

                                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:649036/93‑1 (MQ=255)

                                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               <  2:3688070/93‑1 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:4353274/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:814864/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              <  2:1346112/93‑1 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              <  2:1429314/93‑1 (MQ=255)

                                                         cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             >  1:1494210/1‑93 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  2:4237807/93‑1 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  2:1708520/93‑1 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  1:752164/93‑1 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  1:3336406/1‑93 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  1:3530719/1‑93 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  1:1646231/93‑1 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  1:3631273/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:540373/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  2:2359693/93‑1 (MQ=255)

                                                           agATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:2978527/92‑1 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  2:2425359/93‑1 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  1:2256411/93‑1 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  1:1050645/93‑1 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:4349528/1‑93 (MQ=255)

                                                                  gTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  1:4472136/1‑93 (MQ=255)

                                                                  gTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    <  2:214138/93‑1 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  2:4331029/1‑93 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   <  2:3470284/93‑1 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  1:932953/1‑93 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:529966/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  1:1380590/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  2:1754865/1‑93 (MQ=255)

                                                                       tGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  1:3109339/1‑93 (MQ=255)

                                                                       tGGCTCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  2:4190109/1‑93 (MQ=255)

                                                                        ggCTCCATCAAATCGGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  2:910201/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:4706646/93‑1 (MQ=255)

                                                                            ccATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  2:4168490/1‑93 (MQ=255)

                                                                            ccATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          <  2:1392262/93‑1 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  1:1714908/1‑93 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  1:2124477/93‑1 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  1:764069/1‑93 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        <  2:1850702/93‑1 (MQ=255)

                                                                               tCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:4321262/1‑93 (MQ=255)

                                                                               tCAAAGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:3804775/1‑93 (MQ=255)

                                                                                 aaaGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     >  1:3342276/1‑93 (MQ=255)

                                                                                 aaaGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     >  2:4506940/1‑93 (MQ=255)

                                                                                 aaaGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     <  2:3230536/93‑1 (MQ=255)

                                                                                  aaGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:2676946/1‑93 (MQ=255)

                                                                                   aGCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:2463363/1‑93 (MQ=255)

                                                                                    gCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  >  1:1883538/1‑93 (MQ=255)

                                                                                    gCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:604832/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:1518471/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:3864069/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGGCGGGA‑CGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:133427/93‑1 (MQ=255)

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑GGA‑CG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`