BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	580,773	0	G	A	62.2%	492.4 / inf	479	S66N (AGC→AAC)	ybcX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (97/84); new base A (148/150); total (245/234)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-03
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 495 total aligned reads displayed.

TGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATA  >  NC_000913/580681‑580865

 gCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGGAGc                                                                                             >  1:1267538/1‑93 (MQ=255)

 gCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGc                                                                                             >  1:3394438/1‑93 (MQ=255)

 gCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGc                                                                                             >  2:2074690/1‑93 (MQ=255)

  cTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCg                                                                                            >  1:2816303/1‑93 (MQ=255)

   tAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGAAGCGa                                                                                           >  2:4489172/1‑93 (MQ=255)

   tAATTACTATCTCCCGAAAGAATCCGCATAACAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGa                                                                                           >  1:862031/1‑93 (MQ=255)

    aaTTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGat                                                                                          <  1:4027407/93‑2 (MQ=255)

     aTTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCTCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGatt                                                                                         <  2:1785172/93‑3 (MQ=255)

     aTTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGatt                                                                                         >  1:519400/1‑91 (MQ=255)

      ttACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTa                                                                                        >  1:2677759/1‑93 (MQ=255)

      ttACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTa                                                                                        >  2:1956687/1‑93 (MQ=255)

         cTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACAt                                                                                     <  1:1170288/93‑1 (MQ=255)

          tATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATc                                                                                    <  1:2283956/93‑1 (MQ=255)

            tctcCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCg                                                                                  >  1:2885143/1‑93 (MQ=255)

             ctcCCCAAAGAGTCGGCCTACCAGGAATGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTCCATACGt                                                                                 >  2:3988697/1‑93 (MQ=255)

              tcCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTg                                                                                >  2:1403524/1‑93 (MQ=255)

              tcCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTg                                                                                >  1:2041891/1‑93 (MQ=255)

              tcCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTg                                                                                >  1:3323498/1‑93 (MQ=255)

               ccccAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGa                                                                               <  1:1505060/93‑1 (MQ=255)

               cccGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGa                                                                               <  2:1641596/93‑1 (MQ=255)

                cccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAg                                                                              >  2:2768636/4‑93 (MQ=255)

                cccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAg                                                                              <  1:3144783/90‑1 (MQ=255)

                cccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAg                                                                              >  2:4574038/4‑93 (MQ=255)

                 ccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAgg                                                                             >  1:1574702/3‑93 (MQ=255)

                 cGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAgg                                                                             <  2:2290853/93‑1 (MQ=255)

                  gAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAggg                                                                            >  1:959387/1‑92 (MQ=255)

                  caaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGt                                                                            <  1:4417128/92‑1 (MQ=255)

                   aaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGtc                                                                           >  2:977607/1‑92 (MQ=255)

                    aaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATCAATGCGATGGGTAACGATTACATACGTGAGGtca                                                                          <  2:2977977/93‑3 (MQ=255)

                     aGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGaa                                                                         >  1:4112787/1‑93 (MQ=255)

                         tCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAAtgtg                                                                     >  2:638222/1‑93 (MQ=255)

                         tCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAAtgtg                                                                     >  2:3022393/1‑93 (MQ=255)

                              ctACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTaa                                                                >  2:1523235/2‑93 (MQ=255)

                              aTACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGaa                                                                >  1:3532399/1‑93 (MQ=255)

                              aTACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGaa                                                                <  1:590949/93‑1 (MQ=255)

                               tACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAg                                                               >  1:2778570/1‑93 (MQ=255)

                               tACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGGGAATGTGGCGagg                                                               >  2:3751812/1‑91 (MQ=255)

                                aCCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGt                                                              >  1:4727053/1‑93 (MQ=255)

                                aCCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGt                                                              <  2:512689/93‑1 (MQ=255)

                                  cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                            <  2:3482658/93‑1 (MQ=255)

                                  cAGGAAGGGCGCTGGGAAACACTGCCCTTTCATCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGACGTCt                                                            >  1:3226952/1‑93 (MQ=255)

                                   aGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTg                                                           >  2:4155449/1‑93 (MQ=255)

                                   aGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTg                                                           <  2:3206486/93‑1 (MQ=255)

                                    ggAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                          >  1:1327/1‑93 (MQ=255)

                                    ggAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                          >  2:2392112/1‑93 (MQ=255)

                                    ggAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGc                                                          >  1:2212638/1‑93 (MQ=255)

                                     gAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGcc                                                         <  2:1834568/93‑1 (MQ=255)

                                       aGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCg                                                       >  2:3925055/1‑93 (MQ=255)

                                       aGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCg                                                       >  1:4221073/1‑93 (MQ=255)

                                        gggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgt                                                      <  2:764909/93‑1 (MQ=255)

                                         ggCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtg                                                     <  1:2421666/93‑1 (MQ=255)

                                             cTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCgg                                                 >  1:141191/1‑93 (MQ=255)

                                              tGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGt                                                >  1:4296737/1‑93 (MQ=255)

                                               gggAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTggct                                               >  2:2316051/1‑91 (MQ=255)

                                               gggAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGtt                                               <  1:2937298/93‑1 (MQ=255)

                                                 gAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTAt                                             >  1:1822222/1‑93 (MQ=255)

                                                 gAAACACGGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGTTTAt                                             >  2:599570/1‑93 (MQ=255)

                                                    acacTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTcc                                          <  2:2704109/93‑1 (MQ=255)

                                                    acacTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTcc                                          >  1:3619090/1‑93 (MQ=255)

                                                      gttGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCaaa                                        >  2:2105552/3‑93 (MQ=255)

                                                      acTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCaa                                        >  2:3111147/1‑93 (MQ=255)

                                                        tGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAaaaa                                      <  2:929874/93‑1 (MQ=255)

                                                        tGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCaaaa                                      >  1:251843/1‑93 (MQ=255)

                                                        tGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCaaaa                                      <  2:2855755/93‑1 (MQ=255)

                                                          cccTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAAtg                                    >  1:4175131/1‑93 (MQ=255)

                                                           cgtttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgt                                   <  1:4467958/91‑2 (MQ=255)

                                                             tttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttg                                 <  2:4674188/93‑1 (MQ=255)

                                                              ttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTTTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttgc                                <  1:827022/93‑1 (MQ=255)

                                                              ttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttgc                                <  2:2154969/93‑1 (MQ=255)

                                                              ttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttgc                                <  1:1089926/93‑1 (MQ=255)

                                                               tCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATgttgct                               <  1:388438/93‑1 (MQ=255)

                                                                  gCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTggg                            >  2:3620579/1‑93 (MQ=255)

                                                                    gggCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGgtg                          <  2:3337231/93‑1 (MQ=255)

                                                                      gcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGtt                        <  1:3489920/90‑1 (MQ=255)

                                                                      gCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGtt                        >  2:3670774/1‑93 (MQ=255)

                                                                       cgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGttt                       >  1:1760740/3‑93 (MQ=255)

                                                                        gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                      <  2:256417/92‑1 (MQ=255)

                                                                        gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                      >  2:1123534/2‑93 (MQ=255)

                                                                        gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                      <  2:1191779/92‑1 (MQ=255)

                                                                         atcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTAt                     >  2:2480088/1‑93 (MQ=255)

                                                                         atcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTAt                     >  1:3705964/1‑93 (MQ=255)

                                                                         atcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTAt                     >  1:2141948/1‑93 (MQ=255)

                                                                         atcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTAt                     <  1:4685268/93‑1 (MQ=255)

                                                                         atcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTAt                     >  1:1041698/1‑93 (MQ=255)

                                                                          tcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATg                    <  2:344253/93‑1 (MQ=255)

                                                                            atGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGcg                  >  1:4058843/1‑92 (MQ=255)

                                                                            atGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGcg                  <  2:1010022/93‑2 (MQ=255)

                                                                               aaTGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTat               >  2:3556606/1‑92 (MQ=255)

                                                                               aaTGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTAc               >  2:1272728/1‑93 (MQ=255)

                                                                                aTGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTatt              <  1:3788683/93‑3 (MQ=255)

                                                                                aTGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTatt              <  1:2454402/93‑3 (MQ=255)

                                                                                  gCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTAtttt            >  2:191075/1‑93 (MQ=255)

                                                                                   cGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTta           <  1:4330544/93‑1 (MQ=255)

                                                                                    gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat          >  1:690129/1‑93 (MQ=255)

                                                                                    gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat          <  2:2200689/93‑1 (MQ=255)

                                                                                    gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat          >  1:1945598/1‑93 (MQ=255)

                                                                                      tGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtattc        >  2:3884334/1‑91 (MQ=255)

                                                                                       gggCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATaga       <  2:4248178/93‑1 (MQ=255)

                                                                                        ggCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATagac      >  2:1494498/1‑92 (MQ=255)

                                                                                            gCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATa  >  1:745058/1‑93 (MQ=255)

TGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATA  >  NC_000913/580681‑580865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`