BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	580,783	0	C	A	63.7%	264.5 / inf	477	I69I (ATC→ATA)	ybcX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (96/77); new base A (143/160); total (240/237)
Fisher's exact test for biased strand distribution p-value = 8.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 493 total aligned reads displayed.

TATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAA  >  NC_000913/580691‑580875

tATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATc                                                                                              >  2:2166747/1‑93 (MQ=255)

  tctcCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACg                                                                                            >  2:626131/1‑93 (MQ=255)

    tcCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTg                                                                                          >  1:3526587/1‑93 (MQ=255)

    tcCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTg                                                                                          >  2:1023381/1‑93 (MQ=255)

     ccccAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGa                                                                                         <  1:1505060/93‑1 (MQ=255)

      cccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAg                                                                                        >  2:2768636/4‑93 (MQ=255)

      ccGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAg                                                                                        <  2:4516157/93‑1 (MQ=255)

       ccaaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAgg                                                                                       <  2:1465985/91‑1 (MQ=255)

         aaaGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGtc                                                                                     >  2:977607/1‑92 (MQ=255)

                 ggcctACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACgttgt                                                                             >  2:3699761/5‑93 (MQ=255)

                   cATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGa                                                                           <  1:1942862/93‑1 (MQ=255)

                    aTACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGaa                                                                          <  1:4308913/93‑1 (MQ=255)

                    aTACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGaa                                                                          <  1:590951/93‑1 (MQ=255)

                     tACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAg                                                                         >  1:2778570/1‑93 (MQ=255)

                     tACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAg                                                                         <  2:3397315/93‑1 (MQ=255)

                       ccAGGAAGTGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTc                                                                       <  1:1161568/93‑1 (MQ=255)

                        cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                                      <  2:1814311/93‑1 (MQ=255)

                        cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                                      >  1:4154893/1‑93 (MQ=255)

                        cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                                      <  1:2834089/93‑1 (MQ=255)

                        cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                                      <  2:1236823/93‑1 (MQ=255)

                        cAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCt                                                                      <  2:3482658/93‑1 (MQ=255)

                          ggAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                                    >  1:1327/1‑93 (MQ=255)

                          ggAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                                    >  2:4344424/1‑93 (MQ=255)

                          ggAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGc                                                                    >  1:3589505/1‑93 (MQ=255)

                          ggAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGAGAATGTGGTGAAGTCTGc                                                                    >  1:2305847/1‑93 (MQ=255)

                           gAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGcc                                                                   >  2:551249/1‑93 (MQ=255)

                              gggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgt                                                                <  2:764909/93‑1 (MQ=255)

                              gggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgt                                                                >  1:1068624/1‑93 (MQ=255)

                               ggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgtg                                                               >  2:2299677/1‑93 (MQ=255)

                                gcgcTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgt                                                              <  1:3826544/93‑1 (MQ=255)

                                 cgcTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgtt                                                             <  1:3760906/93‑2 (MQ=255)

                                 cgcTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgtt                                                             <  1:4357130/93‑2 (MQ=255)

                                 cgcTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgtt                                                             <  2:4207431/93‑2 (MQ=255)

                                   cTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCgg                                                           >  1:141191/1‑93 (MQ=255)

                                    tGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGTTGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTggc                                                          >  1:1628659/1‑92 (MQ=255)

                                     gggAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTggct                                                         <  2:1164988/93‑3 (MQ=255)

                                     gggAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTggct                                                         <  2:2835345/93‑3 (MQ=255)

                                       gAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGGAACGATTACATACGTGAGGTCAACGTTGGTAAGTCTGCCCGTGTTGGCTAt                                                       >  1:4709572/1‑93 (MQ=255)

                                       gAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTAt                                                       >  1:1822222/1‑93 (MQ=255)

                                         aaCACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTc                                                     <  1:2436956/93‑1 (MQ=255)

                                            gttGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCaaa                                                  >  1:2187399/3‑93 (MQ=255)

                                              tGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAaaaa                                                <  2:3909614/93‑1 (MQ=255)

                                              tGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAaaaa                                                <  1:203286/93‑1 (MQ=255)

                                              tGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAaaaa                                                <  2:929874/93‑1 (MQ=255)

                                                 ccTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAAtgc                                             >  1:4051217/1‑93 (MQ=255)

                                                  cTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGAGAAGTCTGCCCGTGTCGGTTATTCCAAAAtgct                                            >  2:3310715/1‑93 (MQ=255)

                                                   tttCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAAtgctg                                           >  2:2680524/1‑93 (MQ=255)

                                                   tttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttg                                           >  2:4636007/1‑93 (MQ=255)

                                                    ttCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTTTTAAGTCTGCCCGTGTTGGCTATTCAAAAAtgttgc                                          <  1:827022/93‑1 (MQ=255)

                                                     tCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATgttgct                                         <  1:2901969/93‑1 (MQ=255)

                                                       aGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTgg                                       <  2:2100231/93‑1 (MQ=255)

                                                        gCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTggg                                      >  2:3620579/1‑93 (MQ=255)

                                                         cGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGgt                                     <  1:3329002/93‑1 (MQ=255)

                                                          gggCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGgtg                                    >  1:321118/1‑93 (MQ=255)

                                                           cgcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGgtgt                                   <  1:4568608/89‑1 (MQ=255)

                                                           cgcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGgtgt                                   <  1:3462189/89‑1 (MQ=255)

                                                           cgcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGgtgt                                   <  1:1267558/89‑1 (MQ=255)

                                                            gcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGtt                                  >  2:1721224/4‑93 (MQ=255)

                                                            gcgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGtt                                  >  2:2119768/4‑93 (MQ=255)

                                                             cgatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGttt                                 >  1:1760740/3‑93 (MQ=255)

                                                              gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                                <  2:1102860/92‑1 (MQ=255)

                                                              gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                                <  1:4424550/92‑1 (MQ=255)

                                                              gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                                <  2:1951206/92‑1 (MQ=255)

                                                              gatcatGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTa                                <  2:256417/92‑1 (MQ=255)

                                                              catcatGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTa                                <  1:3961978/93‑1 (MQ=255)

                                                                tcatGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATg                              >  2:3964860/1‑93 (MQ=255)

                                                                tcatGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATg                              >  2:3183023/1‑93 (MQ=255)

                                                                  atGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGcg                            >  1:4058844/1‑92 (MQ=255)

                                                                  atGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                            <  2:2323373/93‑1 (MQ=255)

                                                                  atGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                            <  1:2484544/93‑1 (MQ=255)

                                                                   tGAATGCGATGGGTAACGATTACATACGTGAGGTCCACGTTGTTAAGTCTGCCCGTGTTGGCTTTTCAAAAATGTTGCTGGGTGTTTATgagt                           >  2:151155/1‑90 (MQ=255)

                                                                   tGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGcgt                           >  2:4044173/1‑91 (MQ=255)

                                                                   tGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCt                           >  1:310919/1‑93 (MQ=255)

                                                                   tGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCt                           >  2:3000784/1‑93 (MQ=255)

                                                                      aTGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTatt                        <  2:4690026/93‑3 (MQ=255)

                                                                          gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat                    <  2:2200689/93‑1 (MQ=255)

                                                                          gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat                    >  1:690129/1‑93 (MQ=255)

                                                                          gATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtat                    >  1:225317/1‑93 (MQ=255)

                                                                            tGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTtattc                  >  2:134447/1‑91 (MQ=255)

                                                                            tGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATag                  <  1:4214515/93‑1 (MQ=255)

                                                                             gggCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATaga                 <  2:1574580/93‑1 (MQ=255)

                                                                              ggCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATagag                >  1:3254156/1‑93 (MQ=255)

                                                                                  gCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATa            >  1:745058/1‑93 (MQ=255)

                                                                                  acGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCaca            >  1:2547871/2‑91 (MQ=255)

                                                                                  acGATTACATACGTGAGGGCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCaca            >  2:2935967/2‑91 (MQ=255)

                                                                                   cGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATaa           >  1:1033020/1‑93 (MQ=255)

                                                                                    gattACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAag          >  2:2017665/4‑93 (MQ=255)

                                                                                     attACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagc         <  1:88531/91‑1 (MQ=255)

                                                                                     attACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagc         <  1:970650/91‑1 (MQ=255)

                                                                                     attACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagc         <  2:593271/91‑1 (MQ=255)

                                                                                     attACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagc         <  1:2638339/91‑1 (MQ=255)

                                                                                      ttACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagca        <  1:498797/92‑1 (MQ=255)

                                                                                      ttACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagca        >  1:3662498/2‑93 (MQ=255)

                                                                                      ttACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagca        <  2:1867697/92‑1 (MQ=255)

                                                                                       tACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAagcag       >  2:3566319/1‑93 (MQ=255)

                                                                                       tACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAagcag       >  2:3102840/1‑93 (MQ=255)

                                                                                       tACATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagcag       <  1:2109126/93‑1 (MQ=255)

                                                                                        aCATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAagcagc      >  2:3851848/1‑93 (MQ=255)

                                                                                        aCATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAagcagc      >  1:4275017/1‑93 (MQ=255)

                                                                                         cATACGTGAGGTCAACGTTGTTAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTATGCGTATTTTATTCAGCACAAGCAgcg     >  2:2451093/1‑93 (MQ=255)

TATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAA  >  NC_000913/580691‑580875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`