BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,900	1	.	C	66.7%	209.2 / 117.1	99	intergenic (+234/‑132)	fadL/b2345	FadL/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (10/23); new base C (33/33); total (43/56)
Fisher's exact test for biased strand distribution p-value = 8.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 127 total aligned reads displayed.

GTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGT  >  NC_000913/2460808‑2460990

gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑t                                                                                                 <  1:23676/93‑1 (MQ=255)

gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑t                                                                                                 <  1:3644902/93‑1 (MQ=255)

 tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑c                                                                                               <  2:2543782/93‑1 (MQ=255)

   tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccc                                                                                             <  1:2475833/93‑1 (MQ=255)

     cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc                                                                                           >  2:2023672/1‑93 (MQ=255)

         gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc‑c‑cc‑c                                                                                    >  1:1811440/1‑93 (MQ=255)

         gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc‑c‑cc‑c                                                                                    >  2:3746639/1‑93 (MQ=255)

         gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc‑c‑cc‑c                                                                                    >  1:3982386/1‑93 (MQ=255)

           cAAACGTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTg                                                                                  <  2:208557/93‑1 (MQ=255)

           cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc‑c‑cc‑cct                                                                                  <  1:471315/93‑3 (MQ=255)

             aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑ccccc‑c‑cc‑cctgg                                                                                >  1:2290480/1‑89 (MQ=255)

             aaCCTGAAGGGAAAAGAATATTTTCAGGCGTTTCGGCCGCCCTTTTCAAAGAGTTTTCTTTTCTGCATTTCCCGG‑A‑T‑A‑C‑c‑ccccc‑c‑cc‑cctgg                                                                                <  2:483632/93‑5 (MQ=255)

                 tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTAtt                                                                            <  1:1778414/93‑1 (MQ=255)

                 tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTAtt                                                                            >  2:3080380/1‑93 (MQ=255)

                 cgAAGGGAAATGCATATTTTCAGGCCTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGT‑A‑T‑T‑C‑TCCCCCC‑C‑CC‑CTGGCTAt                                                                             <  2:4290315/92‑1 (MQ=255)

                  gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTAtt                                                                            >  2:4268806/1‑93 (MQ=255)

                  gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATtg                                                                           <  1:3488570/93‑1 (MQ=255)

                   aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATtg                                                                           >  1:3146765/1‑93 (MQ=255)

                    aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATtgtg                                                                         <  1:3556220/93‑1 (MQ=255)

                    aGGGAAAGGCATATTTTCAAGCCTTCTTCCCGTCCTGCTTAAAGAGTTTACTTTTCTGCATTTCCCGG‑G‑T‑ACC‑C‑CCCCC‑C‑CC‑CTGGCTATtgt                                                                          <  2:687964/93‑1 (MQ=255)

                     gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑CACC‑CTCGCTATtgtg                                                                         >  2:3261807/1‑93 (MQ=255)

                      ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTgc                                                                        <  1:1040560/93‑1 (MQ=255)

                      ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTgc                                                                        >  1:467756/1‑93 (MQ=255)

                         aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑Ct                                                                    >  1:364495/1‑93 (MQ=255)

                         aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGCCTATTGTGCG‑Ct                                                                    >  1:2308875/1‑93 (MQ=255)

                            gCATATTTTCGCGCGTTCTGCTCGTCCTTCTCAAAAAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑G‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCAt                                                                 <  2:1039047/93‑1 (MQ=255)

                              atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTCCG‑CTCATac                                                               >  1:2936984/1‑93 (MQ=255)

                                atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATacac                                                             >  1:2881386/1‑93 (MQ=255)

                                atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACt                                                            <  2:2668386/93‑1 (MQ=255)

                                    tCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACCCTCaa                                                         >  2:515300/1‑93 (MQ=255)

                                      aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAAt                                                       >  2:261663/1‑93 (MQ=255)

                                      aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAAt                                                       <  2:3062815/93‑1 (MQ=255)

                                         cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTaa                                                    <  2:75976/93‑1 (MQ=255)

                                          ggtcttctCGTCCTTCTTAAATAGTTTTCTTTTTTGCACTTCCAGG‑A‑T‑C‑CGG‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACACAAATTaaa                                                   <  2:4584601/91‑1 (MQ=255)

                                          gTTCTTCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTaaa                                                   <  2:3281457/93‑1 (MQ=255)

                                          gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTaaa                                                   >  2:350527/1‑93 (MQ=255)

                                          gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATT‑TGCGCCCCATACACCAAAATTaaa                                                   >  1:1385679/1‑93 (MQ=255)

                                          gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAg                                                  <  1:3408991/93‑1 (MQ=255)

                                           ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CCACTGGCTATTTTGCG‑CTCATACACTCCAATTtaaa                                                  >  1:1283545/1‑92 (MQ=255)

                                             cTGGTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGAt                                                <  1:942544/93‑1 (MQ=255)

                                             cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGAt                                                <  1:3176452/93‑1 (MQ=255)

                                              tGCTCGTCCTTCTCAAAGAGTTTGCTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATa                                               <  1:1411602/93‑1 (MQ=255)

                                               gCTCGTCCTTCTCAAAGAGTTTAATTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAg                                              <  2:3391589/93‑1 (MQ=255)

                                                  cGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGtt                                           >  1:3017740/1‑93 (MQ=255)

                                                   gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCt                                         <  1:696807/93‑1 (MQ=255)

                                                    tCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCta                                        >  1:1788358/1‑93 (MQ=255)

                                                     ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCta                                        >  1:2038375/1‑93 (MQ=255)

                                                      cTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaa                                       >  2:3233349/1‑93 (MQ=255)

                                                      cTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaa                                      <  1:2154676/93‑1 (MQ=255)

                                                       ttCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaa                                      <  1:3720125/93‑1 (MQ=255)

                                                        tctcAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTCTTTTGTG‑CCCTTACCCTCAAAATAAAAAATGGTTCtaaaa                                     >  2:206381/1‑92 (MQ=255)

                                                        tctcAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaat                                     <  2:3161637/93‑1 (MQ=255)

                                                         ctcAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaata                                    <  1:2057907/93‑1 (MQ=255)

                                                         ctcAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaata                                    <  2:2066200/93‑1 (MQ=255)

                                                           cAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                  <  1:804907/93‑1 (MQ=255)

                                                           cAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                  <  2:4374149/93‑1 (MQ=255)

                                                           cAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGAAAGGTTTtaaaaaaa                                  >  1:3611183/1‑93 (MQ=255)

                                                            caaGAGTTTTCTTTTTCGCATTTCCAGG‑ATT‑C‑C‑C‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                 <  1:4154615/92‑1 (MQ=255)

                                                            aaaGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                 >  1:445985/1‑93 (MQ=255)

                                                            aaaGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                 <  2:1842657/93‑1 (MQ=255)

                                                             aaGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                               <  1:1417136/93‑1 (MQ=255)

                                                              agagTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                               <  1:1099473/93‑1 (MQ=255)

                                                              agagTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTCGCTATTGTGCG‑CTCATACACTCCAATTAAATATAGGCTCTAAATAAATGa                               >  1:2281810/1‑93 (MQ=255)

                                                              agagTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                              <  2:1049920/93‑1 (MQ=255)

                                                               gagTTTACTTTTCTGCATTTCCAGG‑A‑T‑G‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                              <  1:353843/93‑1 (MQ=255)

                                                               gagTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                              <  1:353842/93‑1 (MQ=255)

                                                                gtttttcttttcTGCATTTCCAGG‑A‑TCC‑C‑C‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc                             <  2:1339166/91‑1 (MQ=255)

                                                                ggTTTACTTTTCTGTATTTCAAGG‑G‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc                             <  2:1339165/92‑1 (MQ=255)

                                                                agTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc                             <  1:252646/93‑1 (MQ=255)

                                                                 gTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAgag                            >  2:4680853/1‑91 (MQ=255)

                                                                 gTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                           >  2:318413/1‑93 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGGG‑CTCATACAATCAAATAAAAGAAAGGTTCTAAATAAATGAGCGt                           >  1:92555/1‑93 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                           >  2:4090038/1‑93 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                           <  2:1059513/93‑1 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                           <  1:2291313/93‑1 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                          >  1:1434755/1‑93 (MQ=255)

                                                                  tttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CCTGCCATTTTGGG‑CTCCATCACTCAAAATTAAAATAAGTTTTAAATTAATGGGCggt                          >  2:4089882/1‑91 (MQ=255)

                                                                   ttACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                         <  2:1176165/93‑1 (MQ=255)

                                                                     tcTTTTCTTCCTTTCCCGGAA‑T‑C‑C‑C‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttt                        <  1:383669/92‑1 (MQ=255)

                                                                       ttttCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                      <  2:1546061/93‑1 (MQ=255)

                                                                       ttttCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                      <  2:2658375/93‑1 (MQ=255)

                                                                       ttttCAGCATTTCCAGA‑A‑T‑A‑A‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                      <  2:1545919/93‑1 (MQ=255)

                                                                        tttCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCGATTGTGCG‑CTCATATACTCAAATTAAAGATTGGTTTTAAATAAATGAGCGttttttt                     >  1:4304500/1‑92 (MQ=255)

                                                                          tCTGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                   <  1:3030916/93‑1 (MQ=255)

                                                                          tCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                  <  1:3258133/93‑1 (MQ=255)

                                                                          tCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                  <  2:3990534/93‑1 (MQ=255)

                                                                           cTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCCTC‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                  <  1:3903256/93‑1 (MQ=255)

                                                                            tGCATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                 <  2:1590674/93‑1 (MQ=255)

                                                                              cATTTCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc               <  2:2249864/93‑1 (MQ=255)

                                                                                tttCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa             <  1:2097449/93‑1 (MQ=255)

                                                                                tttCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAt            <  2:1793252/93‑1 (MQ=255)

                                                                                 ttCCAGG‑A‑T‑ACC‑C‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAt            <  2:3454599/93‑1 (MQ=255)

                                                                                  tCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGTTAGTCtttt           >  2:250859/1‑93 (MQ=255)

                                                                                  tCCAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAtt           <  1:1141391/93‑1 (MQ=255)

                                                                                    cAGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc         >  2:4382657/1‑93 (MQ=255)

                                                                                     aGG‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa        >  2:684060/1‑93 (MQ=255)

                                                                                      gg‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAAATAGGTTCTAAATAAATTAGCGGTTTTTTATTGTTTAtttact       >  2:1991144/1‑90 (MQ=255)

                                                                                      gg‑A‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTCCG‑CTCCGACCCTCAAATTAAAGATAGGTTCTAAATAAATAAGCGTTTTTTGTTAGTCTATTTcct       >  2:2477530/1‑91 (MQ=255)

                                                                                         a‑T‑A‑C‑TCCCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa     <  1:1742250/93‑1 (MQ=255)

                                                                                             a‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt  <  1:671983/93‑1 (MQ=255)

GTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGG‑A‑T‑A‑C‑T‑CCCCC‑C‑CC‑CTGGCTATTGTGCG‑CTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGT  >  NC_000913/2460808‑2460990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`