BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,445	T→C	N265S (AAC→AGC)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,445	0	T	C	97.1%	535.3 / 2.7	174	N265S (AAC→AGC)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (89/80); total (93/81)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 175 total aligned reads displayed.

ACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCC  >  NC_000913/3897359‑3897534

aCCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCtcatca                                                                                     <  1:2317805/93‑1 (MQ=255)

 cccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAg                                                                                    <  1:2209544/93‑1 (MQ=255)

 cccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAg                                                                                    >  2:1578962/1‑93 (MQ=255)

 accGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAg                                                                                    <  1:2236879/92‑1 (MQ=255)

  ccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGt                                                                                   >  2:3099534/1‑93 (MQ=255)

  ccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGt                                                                                   >  1:531595/1‑93 (MQ=255)

     ccTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTcg                                                                                >  2:3252218/1‑93 (MQ=255)

     ccTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTcg                                                                                >  2:3252219/1‑93 (MQ=255)

      cTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTcgc                                                                               <  1:2354849/93‑1 (MQ=255)

        tgtgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGa                                                                             >  1:2785323/1‑93 (MQ=255)

        tgtgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGa                                                                             <  2:4281061/93‑1 (MQ=255)

         gtgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGAt                                                                            <  1:4707122/93‑1 (MQ=255)

          tgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATc                                                                           <  2:1521031/93‑1 (MQ=255)

          tgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATc                                                                           <  1:950774/93‑1 (MQ=255)

          tgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATc                                                                           <  2:1929375/93‑1 (MQ=255)

          tgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATc                                                                           <  1:8912/93‑1 (MQ=255)

          tgAAAGCACTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATc                                                                           <  1:1024555/93‑1 (MQ=255)

             aaGCCCTGCCAGCGCCGGGCCATTCGAATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGc                                                                        <  2:1326096/93‑1 (MQ=255)

                cccTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGc                                                                     <  1:1204130/93‑1 (MQ=255)

                  cTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCtt                                                                   <  2:4499140/93‑1 (MQ=255)

                   tGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAATCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCttt                                                                  <  2:2621659/93‑1 (MQ=255)

                    gCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTa                                                                 >  2:2988029/1‑93 (MQ=255)

                    gCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTa                                                                 <  2:4332932/93‑1 (MQ=255)

                     ccAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAg                                                                >  1:3149280/1‑93 (MQ=255)

                        gcgcgcGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCAt                                                             >  1:2506993/1‑93 (MQ=255)

                           cgcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCa                                                          >  1:3823573/1‑93 (MQ=255)

                           cgcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCAGCTTTAGCATTcc                                                          >  2:4329156/1‑92 (MQ=255)

                            gcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAg                                                         <  1:3004448/93‑1 (MQ=255)

                            gcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAg                                                         <  2:412207/93‑1 (MQ=255)

                            gcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAg                                                         <  2:1399433/93‑1 (MQ=255)

                             cgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGc                                                        >  1:29526/1‑93 (MQ=255)

                             cgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGc                                                        <  2:1857049/93‑1 (MQ=255)

                                 cATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGAt                                                    <  1:4633625/93‑1 (MQ=255)

                                  aTCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATa                                                   >  1:1666365/1‑93 (MQ=255)

                                   tCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaa                                                  >  1:222044/1‑93 (MQ=255)

                                      gCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAg                                               >  2:3435594/1‑93 (MQ=255)

                                      gCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAg                                               >  1:2152152/1‑93 (MQ=255)

                                       cATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGa                                              <  2:1708364/93‑1 (MQ=255)

                                        aTCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGAc                                             >  1:3659107/1‑93 (MQ=255)

                                         tCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACt                                            >  2:4069934/1‑93 (MQ=255)

                                          cTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACtt                                           >  2:2882157/1‑93 (MQ=255)

                                           tttACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                          >  2:739797/1‑93 (MQ=255)

                                           tttACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                          >  1:3947353/1‑93 (MQ=255)

                                           tttACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                          >  1:1721024/1‑93 (MQ=255)

                                           tttACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                          >  1:1284521/1‑93 (MQ=255)

                                            ttACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTa                                         >  1:2853394/1‑93 (MQ=255)

                                             tACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTAc                                        >  1:4173765/1‑93 (MQ=255)

                                               cGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGt                                      >  2:218582/1‑93 (MQ=255)

                                                 gACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGTTAAAAGACTTTACGTCt                                    <  2:2668981/93‑1 (MQ=255)

                                                 gACATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCt                                    <  2:2668982/93‑1 (MQ=255)

                                                   cATTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCttt                                  <  2:4141205/93‑1 (MQ=255)

                                                    aTTAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTc                                 <  1:2733449/93‑1 (MQ=255)

                                                     ttAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTcc                                <  1:3763753/93‑1 (MQ=255)

                                                     ttAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTcc                                <  1:2452368/93‑1 (MQ=255)

                                                      tAAAACGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCt                               <  2:4451266/93‑1 (MQ=255)

                                                       aaaaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTg                              >  2:2491696/1‑93 (MQ=255)

                                                        aaaCGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCtta                             <  2:1559513/93‑3 (MQ=255)

                                                        aaaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGa                             <  2:3506726/93‑1 (MQ=255)

                                                        aaaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGa                             <  1:4450279/93‑1 (MQ=255)

                                                        aaaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGa                             <  1:3315633/93‑1 (MQ=255)

                                                         aaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAg                            >  1:3517291/1‑93 (MQ=255)

                                                         aaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAg                            <  1:3320567/93‑1 (MQ=255)

                                                         aaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAg                            >  1:1630536/1‑93 (MQ=255)

                                                         aaCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAg                            <  2:2103250/93‑1 (MQ=255)

                                                          aCGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAgg                           <  1:2104993/93‑1 (MQ=255)

                                                           cGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGc                          >  2:4190311/1‑93 (MQ=255)

                                                           cGCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGc                          >  1:6402/1‑93 (MQ=255)

                                                            gCTTGCTAATCAGCGGGATAATATCGCTTATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGTTAAAAGACTTTACGTCTTTCCTGAggga                         >  2:3694988/1‑91 (MQ=255)

                                                            gCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCa                         >  2:106743/1‑93 (MQ=255)

                                                            gCTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCa                         >  1:2412317/1‑93 (MQ=255)

                                                             cTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCAc                        >  2:4369469/1‑93 (MQ=255)

                                                             cTTGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCAc                        <  2:3168987/93‑1 (MQ=255)

                                                              ttGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACg                       >  2:1428177/1‑93 (MQ=255)

                                                              ttGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACg                       <  2:3040825/93‑1 (MQ=255)

                                                               tGCTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGa                      <  1:4215524/93‑1 (MQ=255)

                                                                 cTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGaaa                    >  1:1553253/1‑93 (MQ=255)

                                                                 cTAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGaaa                    >  2:4653652/1‑93 (MQ=255)

                                                                  tAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGCCTTTACGTCTTTCCTGAGGCACGAAAt                   <  1:509780/93‑1 (MQ=255)

                                                                  tAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAAt                   >  1:776052/1‑93 (MQ=255)

                                                                  tAATCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAAt                   <  2:38526/93‑1 (MQ=255)

                                                                     tCAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCtt                <  1:10224/93‑1 (MQ=255)

                                                                      cAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGTATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTc               <  1:3661939/93‑1 (MQ=255)

                                                                      cAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGACATCTTc               >  2:1404132/1‑93 (MQ=255)

                                                                      cAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTc               <  2:243139/93‑1 (MQ=255)

                                                                      cAGCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTc               <  2:2328127/93‑1 (MQ=255)

                                                                        gCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCgg             >  2:3671327/1‑93 (MQ=255)

                                                                        gCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCgg             <  2:2422852/93‑1 (MQ=255)

                                                                        gCGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCgg             >  1:3425867/1‑93 (MQ=255)

                                                                         cGGGATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCggg            >  1:3808402/1‑93 (MQ=255)

                                                                          gggATAATATCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGa           <  1:643852/93‑1 (MQ=255)

                                                                             ataataTCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaata        <  1:4076729/93‑1 (MQ=255)

                                                                              taataTCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaataa       >  2:2800503/1‑93 (MQ=255)

                                                                              taataTCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaataa       <  1:381550/93‑1 (MQ=255)

                                                                              taataTCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaataa       <  1:2001549/93‑1 (MQ=255)

                                                                              taataTCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaataa       <  1:3146682/93‑1 (MQ=255)

                                                                                atatCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATg     >  1:1823387/1‑93 (MQ=255)

                                                                                 tatCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGc    <  2:1163625/93‑1 (MQ=255)

                                                                                  atCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGcc   <  1:4414219/93‑1 (MQ=255)

                                                                                   tCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGccc  >  1:2717714/1‑93 (MQ=255)

                                                                                   tCGCTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGccc  <  2:4679580/93‑1 (MQ=255)

ACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCC  >  NC_000913/3897359‑3897534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`