BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,211	T→C	T473A (ACC→GCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,211	0	T	C	98.6%	468.1 / ‑3.4	147	T473A (ACC→GCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (66/78); total (68/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 147 total aligned reads displayed.

CTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCG  >  NC_000913/482121‑482299

cTTTGGCTATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCaa                                                                                        >  2:609557/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                       >  2:1371223/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                       >  2:1735385/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                       <  1:1230153/93‑1 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                       >  1:137580/1‑93 (MQ=255)

 tttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAAt                                                                                       <  2:3963431/93‑1 (MQ=255)

  ttGTCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCCCCATTGCTGAAACAATGGCAATa                                                                                      <  2:2182468/93‑1 (MQ=255)

  ttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATa                                                                                      <  1:4524422/93‑1 (MQ=255)

  ttGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATa                                                                                      >  1:3560082/1‑93 (MQ=255)

     gCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATagag                                                                                   <  1:3041771/93‑1 (MQ=255)

      cAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAgaga                                                                                  <  2:2159719/93‑1 (MQ=255)

      cAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAgaga                                                                                  >  1:795836/1‑93 (MQ=255)

       aaTCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGaa                                                                                 >  1:2093770/1‑93 (MQ=255)

        aTCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAAc                                                                                >  1:3213009/1‑93 (MQ=255)

         tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACt                                                                               >  2:808677/1‑93 (MQ=255)

         tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACt                                                                               >  2:565319/1‑93 (MQ=255)

         tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACt                                                                               <  2:3013353/93‑1 (MQ=255)

          cGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTg                                                                              >  2:2675344/1‑93 (MQ=255)

           ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGa                                                                             <  1:2260322/93‑1 (MQ=255)

           ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGa                                                                             <  2:3909412/93‑1 (MQ=255)

           ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGACAACTGa                                                                             <  2:3382898/93‑1 (MQ=255)

            gTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGAc                                                                            >  2:2857347/1‑93 (MQ=255)

            gTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGAc                                                                            <  1:1292763/93‑1 (MQ=255)

             tttCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACg                                                                           >  1:4369298/1‑93 (MQ=255)

              ttCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACga                                                                          <  2:3269877/93‑1 (MQ=255)

              ttCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACga                                                                          <  2:1506835/93‑1 (MQ=255)

                cAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCGTTGCTGAAACAATGGCAATAGAGAACTGACgata                                                                        <  2:4320530/93‑1 (MQ=255)

                cAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata                                                                        <  2:2332850/93‑1 (MQ=255)

                cAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgata                                                                        >  2:2109113/1‑93 (MQ=255)

                 aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatag                                                                       <  1:3175300/93‑1 (MQ=255)

                 aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatag                                                                       <  2:2359786/93‑1 (MQ=255)

                    aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACgatagata                                                                    >  1:3294991/1‑93 (MQ=255)

                     tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGatagatag                                                                   >  2:647356/1‑93 (MQ=255)

                      ggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGc                                                                  <  1:3352903/93‑1 (MQ=255)

                      ggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGc                                                                  <  1:2299848/93‑1 (MQ=255)

                          gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCa                                                              <  1:2603234/93‑1 (MQ=255)

                             caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTa                                                           <  2:548299/93‑1 (MQ=255)

                             caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTa                                                           >  1:607316/1‑93 (MQ=255)

                               aaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGa                                                         >  1:3779911/1‑93 (MQ=255)

                                agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                        <  1:704439/93‑1 (MQ=255)

                                agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGaa                                                        >  2:3935018/1‑93 (MQ=255)

                                 gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAAc                                                       <  1:1613718/93‑1 (MQ=255)

                                   gCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCg                                                     <  2:2518165/93‑1 (MQ=255)

                                      ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                  >  1:2731926/1‑93 (MQ=255)

                                      ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                  >  2:2087735/1‑93 (MQ=255)

                                      ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa                                                  >  2:785799/1‑93 (MQ=255)

                                       gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaa                                                 <  2:1294277/93‑1 (MQ=255)

                                       gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaa                                                 >  2:205563/1‑93 (MQ=255)

                                         gTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                               >  2:3608616/1‑93 (MQ=255)

                                          tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                              <  1:2860177/93‑1 (MQ=255)

                                          tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga                                              >  1:647665/1‑93 (MQ=255)

                                           cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa                                             >  2:1217690/1‑93 (MQ=255)

                                            aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACGGACGATAGATAGCACCAGTAGAACCGCCAaagaag                                            <  1:1248137/93‑1 (MQ=255)

                                            aGGATCAACGCCAACAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaag                                            <  1:3062189/93‑1 (MQ=255)

                                              gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc                                          <  1:972468/93‑1 (MQ=255)

                                                  aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATc                                      >  2:2800899/1‑93 (MQ=255)

                                                  aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATc                                      <  1:1025940/93‑1 (MQ=255)

                                                   aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg                                     >  2:2027004/1‑93 (MQ=255)

                                                   aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg                                     >  1:1237625/1‑93 (MQ=255)

                                                     gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt                                   <  2:3055233/93‑1 (MQ=255)

                                                      ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                  <  1:1380170/93‑1 (MQ=255)

                                                       caccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                 <  2:4139135/93‑1 (MQ=255)

                                                       caccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                 <  2:2079417/93‑1 (MQ=255)

                                                        accaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg                                >  1:3320859/1‑93 (MQ=255)

                                                         ccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGa                               >  2:4288368/1‑93 (MQ=255)

                                                         ccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGa                               >  1:3381978/1‑93 (MQ=255)

                                                         ccaGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGa                               <  2:2475220/93‑1 (MQ=255)

                                                          caGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                              <  2:3711095/93‑1 (MQ=255)

                                                          caGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa                              <  2:1039687/93‑1 (MQ=255)

                                                           aGTACCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAAt                             >  1:4056500/1‑93 (MQ=255)

                                                             gaCCGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCCCCAGTAGACCCGCCAAAGAAGGCCATCGGTACGAAtgc                           <  2:3882096/92‑3 (MQ=255)

                                                              aCCGACATCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATATATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGGACGAATAcc                          >  2:1237442/1‑93 (MQ=25)

                                                              aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcg                          >  2:2014647/1‑92 (MQ=255)

                                                                cGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc                        <  2:3004536/93‑1 (MQ=255)

                                                                cGACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc                        <  2:4421461/93‑1 (MQ=255)

                                                                 gACAGCGCCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgcc                       <  1:1090086/93‑1 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                   >  1:1208566/1‑93 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                   >  1:1338434/1‑93 (MQ=255)

                                                                     gcgcCATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                   <  1:4134503/93‑1 (MQ=255)

                                                                        ccATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAGCGCCGACAGTa                >  1:4170323/1‑93 (MQ=255)

                                                                         cATTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc               <  2:1968731/93‑1 (MQ=255)

                                                                          aTTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc              >  2:3683537/1‑93 (MQ=255)

                                                                          aTTGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc              >  1:2135682/1‑93 (MQ=255)

                                                                          aTTGCTGAAACAATGGCAATAGAGAACTAACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc              >  1:1615774/1‑93 (MQ=255)

                                                                           ttGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa             <  2:2456534/93‑1 (MQ=255)

                                                                            tGCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt            <  2:4656470/93‑1 (MQ=255)

                                                                             gCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGACCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc           <  2:1201286/93‑1 (MQ=255)

                                                                             gCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc           <  1:3604836/93‑1 (MQ=255)

                                                                             gCTGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc           <  2:1926895/93‑1 (MQ=255)

                                                                               tGAAACAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc         >  1:1112702/1‑93 (MQ=255)

                                                                                 aaaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa       >  1:1046851/1‑93 (MQ=255)

                                                                                  aaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt      >  1:1016054/1‑93 (MQ=255)

                                                                                  aaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt      >  2:449788/1‑93 (MQ=255)

                                                                                  aaCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt      <  1:4091679/93‑1 (MQ=255)

                                                                                   aCAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa     <  2:3715110/93‑1 (MQ=255)

                                                                                    cAATGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAc    <  1:2167861/93‑1 (MQ=255)

                                                                                     aaTGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc   >  2:3067330/1‑93 (MQ=255)

                                                                                     aaTGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc   <  2:2843605/93‑1 (MQ=255)

                                                                                      aTGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCg  <  2:3863186/93‑1 (MQ=255)

                                                                                      aTGGCAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCg  <  1:2425377/93‑1 (MQ=255)

CTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCG  >  NC_000913/482121‑482299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`