BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,742	T→C	A320A (GCT→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,742	0	T	C	98.6%	433.3 / ‑2.5	139	A320A (GCT→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (64/73); total (65/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

Only 100 of 146 total aligned reads displayed.

ATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925653‑3925831

aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  2:2084308/1‑93 (MQ=255)

   ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGtt                                                                                     <  1:4253958/93‑1 (MQ=255)

   ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGtt                                                                                     <  1:618667/93‑1 (MQ=255)

   ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGtt                                                                                     >  1:1905818/1‑93 (MQ=255)

    gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  2:3078185/93‑1 (MQ=255)

    gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  1:1627516/93‑1 (MQ=255)

      ggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgc                                                                                  >  1:3703462/1‑93 (MQ=255)

      ggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgc                                                                                  <  2:1209462/93‑1 (MQ=255)

         ggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgag                                                                               >  1:2487825/1‑93 (MQ=255)

          gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgagg                                                                              >  1:1531231/1‑92 (MQ=255)

          gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              <  2:1284776/93‑1 (MQ=255)

          gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              <  2:3747480/93‑1 (MQ=255)

            ctCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCGGCTGCTGTTCGCGAGAGc                                                                            <  2:3866732/92‑1 (MQ=255)

                 ccAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTccc                                                                       <  1:2175173/93‑1 (MQ=255)

                  cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCt                                                                      <  2:2815209/93‑1 (MQ=255)

                   aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  2:3681862/1‑93 (MQ=255)

                   aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  2:4277584/1‑93 (MQ=255)

                   aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  1:1653304/1‑93 (MQ=255)

                   aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGGTCGAGCGAGCGTcgtct                                                                     >  2:4277583/1‑89 (MQ=255)

                      ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  <  2:1234828/93‑1 (MQ=255)

                       tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  1:2632103/1‑93 (MQ=255)

                       tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 <  2:4590038/93‑1 (MQ=255)

                       tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 <  1:1657940/93‑1 (MQ=255)

                         gTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgc                                                               >  1:446554/1‑93 (MQ=255)

                          tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              >  1:4049243/1‑93 (MQ=255)

                          tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              <  2:55385/93‑1 (MQ=255)

                          tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              >  1:4049242/1‑93 (MQ=255)

                            tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            >  2:3566704/1‑93 (MQ=255)

                            tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            <  1:1795117/93‑1 (MQ=255)

                            tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGt                                                            <  2:2776176/93‑1 (MQ=255)

                              aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  2:801070/1‑93 (MQ=255)

                               cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         <  1:2563997/93‑1 (MQ=255)

                                tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  1:37810/1‑93 (MQ=255)

                                   gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     >  1:296041/1‑93 (MQ=255)

                                    gcgctgctgcAACTGCCGCATAGCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAttt                                                    <  1:1066706/91‑1 (MQ=255)

                                     tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   >  1:1325634/1‑93 (MQ=255)

                                     tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   <  2:342456/93‑1 (MQ=255)

                                      gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAt                                                  <  1:2926846/93‑1 (MQ=255)

                                       ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATc                                                 <  1:859898/93‑1 (MQ=255)

                                       ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATc                                                 <  1:859897/93‑1 (MQ=255)

                                         gctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgc                                               >  1:1051554/1‑93 (MQ=255)

                                          ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              >  1:303713/1‑93 (MQ=255)

                                            gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccgc                                            >  1:4048162/1‑93 (MQ=255)

                                            gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccgc                                            >  2:883661/1‑93 (MQ=255)

                                             cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCgccgcc                                           >  2:2689238/1‑93 (MQ=255)

                                              aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCa                                          >  2:3147350/1‑93 (MQ=255)

                                                cTGCCGCATATCGGGCAGGTTCAGTGTGGAGTATGGCAAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        <  1:3850488/93‑1 (MQ=255)

                                                cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        >  2:2820266/1‑93 (MQ=255)

                                                cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        <  1:3768633/93‑1 (MQ=255)

                                                cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        >  1:3396242/1‑93 (MQ=255)

                                                 tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                       >  1:3660398/1‑93 (MQ=255)

                                                 tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                       >  1:64271/1‑93 (MQ=255)

                                                  gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      <  2:1460114/93‑1 (MQ=255)

                                                  gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      >  2:4598238/1‑93 (MQ=255)

                                                  gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      <  2:980210/93‑1 (MQ=255)

                                                   ccGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTc                                     <  1:1049507/93‑1 (MQ=255)

                                                   ccGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTc                                     <  1:1049364/93‑1 (MQ=255)

                                                     gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  1:4117269/1‑93 (MQ=255)

                                                      cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGa                                  >  1:2416947/1‑93 (MQ=255)

                                                       atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 <  1:4494683/93‑1 (MQ=255)

                                                          tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              <  2:1099596/93‑1 (MQ=255)

                                                          tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              >  1:3971132/1‑93 (MQ=255)

                                                          tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              <  2:1949580/93‑1 (MQ=255)

                                                          tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              <  1:550856/93‑1 (MQ=255)

                                                           cGGCCAGGTTCATGTTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                             <  1:2356090/93‑1 (MQ=255)

                                                           cGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                             >  1:3460819/1‑93 (MQ=255)

                                                           cGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                             <  1:1136916/93‑1 (MQ=255)

                                                           cGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                             <  2:521624/93‑1 (MQ=255)

                                                            ggCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                            >  2:3376720/1‑93 (MQ=255)

                                                            ggCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                            >  1:2761982/1‑93 (MQ=255)

                                                            ggCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                            <  1:1864541/93‑1 (MQ=255)

                                                             gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTCATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  1:4472023/1‑93 (MQ=255)

                                                                aGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCGGCGTCTGAGCAGGCGGCt                        >  2:4353527/1‑93 (MQ=255)

                                                                aGGTTCAGTGTGGAGTATGGCCAGCCGCTGGTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGTCt                        >  2:3731105/1‑93 (MQ=255)

                                                                 ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       >  1:292714/1‑93 (MQ=255)

                                                                 ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       <  2:2568407/93‑1 (MQ=255)

                                                                    tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    <  1:4503306/93‑1 (MQ=255)

                                                                    tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    >  2:972933/1‑93 (MQ=255)

                                                                    tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    >  2:3477414/1‑93 (MQ=255)

                                                                    tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    <  2:1858975/93‑1 (MQ=255)

                                                                     cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:1269183/93‑1 (MQ=255)

                                                                     cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:805305/93‑1 (MQ=255)

                                                                     cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:3027221/93‑1 (MQ=255)

                                                                      aGTGTGGAGGATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTAGCAt                  >  1:2452165/1‑93 (MQ=255)

                                                                        tgtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                <  2:4418174/93‑1 (MQ=255)

                                                                         gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               <  2:3515181/93‑1 (MQ=255)

                                                                         gtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc               <  2:2146046/93‑1 (MQ=255)

                                                                           ggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCgg             >  1:2185101/1‑93 (MQ=255)

                                                                           ggAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCgg             <  1:4229/93‑1 (MQ=255)

                                                                            gAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGt            <  2:3028206/93‑1 (MQ=255)

                                                                            gAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGt            >  2:1416499/1‑93 (MQ=255)

                                                                             aGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  1:276267/93‑1 (MQ=255)

                                                                              gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          <  2:2728038/93‑1 (MQ=255)

                                                                               tATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATc         >  2:3913380/1‑93 (MQ=255)

                                                                                aTGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa        <  2:143730/93‑1 (MQ=255)

                                                                                 tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       <  1:4608798/93‑1 (MQ=255)

                                                                                  ggCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaaa      <  1:4658899/93‑1 (MQ=255)

                                                                                    gcacctgttgTTCGCGCGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    <  1:4110431/89‑1 (MQ=255)

                                                                                    ccAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    <  1:777216/93‑1 (MQ=255)

                                                                                      aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  <  2:4079691/93‑1 (MQ=255)

ATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925653‑3925831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`