BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	493,536	C→T	D79D (GAC→GAT)	ybaB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	493,536	0	C	T	97.8%	620.9 / ‑0.8	178	D79D (GAC→GAT)	ybaB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (76/98); total (79/99)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 183 total aligned reads displayed.

ACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGT  >  NC_000913/493446‑493624

acacaACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGACGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGGAGCATTCAACGATgc                                                                                        >  1:1040416/1‑93 (MQ=255)

 cacaACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATgca                                                                                       <  2:2516544/93‑1 (MQ=255)

   caACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATgcagc                                                                                     >  2:2646747/1‑93 (MQ=255)

    aaCTGCCGTCGCGTAGAGATCGACACGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCTACGCTgcagca                                                                                    >  2:1632129/1‑93 (MQ=255)

         ccGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCGGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCAcgtcg                                                                               <  1:2527198/93‑1 (MQ=255)

           gTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTa                                                                             <  2:3858387/93‑1 (MQ=255)

           gTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTa                                                                             >  2:3659650/1‑93 (MQ=255)

           gTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTa                                                                             <  2:2057949/93‑1 (MQ=255)

           gTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTa                                                                             >  1:2299857/1‑93 (MQ=255)

           gTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTa                                                                             <  1:2179967/93‑1 (MQ=255)

            tCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTAt                                                                            >  1:2819005/1‑93 (MQ=255)

            tCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTAt                                                                            >  1:1325082/1‑93 (MQ=255)

            tCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTAt                                                                            <  2:1463437/93‑1 (MQ=255)

              gcgTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTg                                                                          >  1:329956/1‑93 (MQ=255)

               cgTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTga                                                                         <  1:3992273/93‑1 (MQ=255)

                gTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTgaa                                                                        <  2:2938692/93‑1 (MQ=255)

                  agagATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTgaaga                                                                      <  2:2689445/93‑1 (MQ=255)

                  agagATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTgaaga                                                                      <  1:711887/93‑1 (MQ=255)

                   gagaTCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTgaagaa                                                                     >  1:4494660/1‑93 (MQ=255)

                   gagaTCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTgaagaa                                                                     >  1:4494659/1‑93 (MQ=255)

                    agaTCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGaaa                                                                    >  1:88648/1‑93 (MQ=255)

                    agaTCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGaaa                                                                    <  1:3086723/93‑1 (MQ=255)

                     gaTCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAAc                                                                   <  1:3047690/93‑1 (MQ=255)

                       tCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGc                                                                 >  2:668493/1‑93 (MQ=255)

                        cGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCa                                                                <  2:1917573/93‑1 (MQ=255)

                        cGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCa                                                                <  2:1972887/93‑1 (MQ=255)

                        cGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCa                                                                <  2:3373525/93‑1 (MQ=255)

                         gACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCag                                                               >  1:79105/1‑93 (MQ=255)

                           cccGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaa                                                             >  1:1134302/1‑93 (MQ=255)

                           cccGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaa                                                             <  2:3730174/93‑1 (MQ=255)

                            ccGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaaa                                                            <  1:3428480/93‑1 (MQ=255)

                            ccGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaaa                                                            <  1:2014140/93‑1 (MQ=255)

                             cGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaaag                                                           >  1:930893/1‑93 (MQ=255)

                              gAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCagaaaga                                                          <  1:2345463/93‑1 (MQ=255)

                                gCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAgaaagaaa                                                        <  2:39793/93‑1 (MQ=255)

                                gCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAgaaagaaa                                                        <  1:1479170/93‑1 (MQ=255)

                                 ccTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGaaaa                                                       >  2:3964878/1‑93 (MQ=255)

                                 ccTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGaaaa                                                       >  2:1811219/1‑93 (MQ=255)

                                   tgctgGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGaaaaaa                                                     >  1:2974616/1‑93 (MQ=255)

                                   tgctgGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGaaaaaa                                                     <  1:3076515/93‑1 (MQ=255)

                                   tgctgGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGaaaaaa                                                     <  2:2773022/93‑1 (MQ=255)

                                     ctgGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATg                                                   >  1:4364131/1‑93 (MQ=255)

                                      tgGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATgg                                                  <  2:4681688/93‑1 (MQ=255)

                                       ggAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGc                                                 >  2:189542/1‑93 (MQ=255)

                                       ggAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGc                                                 <  2:4464612/93‑1 (MQ=255)

                                       ggAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGc                                                 >  1:2729914/1‑93 (MQ=255)

                                       ggAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGc                                                 >  1:3665815/1‑93 (MQ=255)

                                       ggAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGc                                                 >  1:4715592/1‑93 (MQ=255)

                                        gAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGcc                                                <  2:956091/93‑1 (MQ=255)

                                        gAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGcc                                                >  1:3250360/1‑93 (MQ=255)

                                         aaGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCct                                               <  2:2706621/93‑1 (MQ=255)

                                         aaGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCct                                               >  2:1387278/1‑93 (MQ=255)

                                           gacgacAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCctct                                             >  1:2784186/1‑93 (MQ=255)

                                              gacAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTa                                          <  2:2416122/93‑1 (MQ=255)

                                              gacAAAGAGATGCTGGAAGACCGTGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTa                                          <  1:1220463/93‑1 (MQ=255)

                                               acAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTAt                                         <  2:2830269/93‑1 (MQ=255)

                                                 aaaGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTAtcc                                       >  2:2102653/1‑93 (MQ=255)

                                                  aaGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTAtcct                                      <  2:3077305/93‑1 (MQ=255)

                                                  aaGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTAtcct                                      <  1:2023638/93‑1 (MQ=255)

                                                  aaGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGCCGTATTGAAGAAAGGCAGAAAGAAAAAATGAGCTCTGGAgcct                                      >  1:2674955/1‑93 (MQ=255)

                                                     agaTGCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCg                                   <  2:1003609/93‑1 (MQ=255)

                                                         gCTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAAt                               <  1:804035/93‑1 (MQ=255)

                                                          cTGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATg                              >  2:2223758/1‑93 (MQ=255)

                                                           tGGAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGc                             <  1:663493/93‑1 (MQ=255)

                                                            ggAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCa                            <  2:779861/93‑1 (MQ=255)

                                                            ggAAGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCa                            <  1:3966197/93‑1 (MQ=255)

                                                              aaGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGc                          <  1:2242555/93‑1 (MQ=255)

                                                               aGACCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCt                         <  1:4593076/93‑1 (MQ=255)

                                                               aGACCTGGGGCCTGCAGCATTCAACGATGCAGCACGTCGCATTGAAGAAACGCAGAAAGAAAAAATGGACTCTGGATCCTCCGGAATCCAGcg                         >  2:1114122/1‑92 (MQ=255)

                                                                 acatggttgCTGCAGCATTCAACTATGCAGCGCGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgc                       <  2:3533112/90‑1 (MQ=255)

                                                                 aCCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgc                       <  1:1763078/93‑1 (MQ=255)

                                                                 aCCTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgc                       >  2:2574699/1‑93 (MQ=255)

                                                                  ccTGGTGGCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgcc                      >  2:3602823/1‑93 (MQ=255)

                                                                     ggtggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgccgcc                   <  1:3575503/93‑1 (MQ=255)

                                                                     ggtggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgccgcc                   <  1:4619892/93‑1 (MQ=255)

                                                                     ggtggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgccgcc                   <  1:1140850/93‑1 (MQ=255)

                                                                     ggtggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTgccgcc                   >  1:1963218/1‑93 (MQ=255)

                                                                      gtggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCt                  <  2:1803141/93‑1 (MQ=255)

                                                                       tggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTg                 <  1:3325698/93‑1 (MQ=255)

                                                                       tggCTGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTg                 <  2:2192139/93‑1 (MQ=255)

                                                                           tGCAGCATTCAATGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAATAAGGCCTCTGTATCTTCCGGAATGCAGCGGCCGCCTGGCtt             >  2:4225050/1‑93 (MQ=255)

                                                                           tGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCtt             >  1:3923588/1‑93 (MQ=255)

                                                                           tGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCtt             >  1:4430121/1‑93 (MQ=255)

                                                                           tGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCtt             <  1:1006463/93‑1 (MQ=255)

                                                                           tGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCtt             <  2:1617370/93‑1 (MQ=255)

                                                                           tGCAGCATTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCtt             <  2:2281177/93‑1 (MQ=255)

                                                                            gcagcaTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCttt            <  1:656969/93‑1 (MQ=255)

                                                                            gcagcaTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCttt            >  2:3038931/1‑93 (MQ=255)

                                                                              agcaTTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCGCCGGAATGCAGCTGCCGCCTGGCTTTaa          >  1:1587386/1‑93 (MQ=255)

                                                                               gcaTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAg         >  2:2664947/1‑93 (MQ=255)

                                                                                caTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGa        <  1:4148388/93‑1 (MQ=255)

                                                                                 aTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGAt       <  1:4125732/93‑1 (MQ=255)

                                                                                 aTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGAt       <  1:4147807/93‑1 (MQ=255)

                                                                                 aTTCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGAt       >  1:4665515/1‑93 (MQ=255)

                                                                                  ttCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATg      <  2:1679112/93‑1 (MQ=255)

                                                                                  ttCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCATGGCTTTAAGATg      >  1:3469977/1‑93 (MQ=255)

                                                                                   tCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGc     <  1:1804838/93‑1 (MQ=255)

                                                                                   tCAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGc     <  2:2142684/93‑1 (MQ=255)

                                                                                    cAACGATGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGcc    >  2:1851874/1‑93 (MQ=255)

                                                                                     aaCGATGCAGCACGTCGTATTGAAGAAACGCAGGAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGcct   >  2:761962/1‑92 (MQ=255)

ACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGT  >  NC_000913/493446‑493624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`