Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,531	T→C	noncoding (206/2906 nt)	rrlC →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,531	0	T	C	100.0%	41.7 / NA	20	noncoding (206/2906 nt)	rrlC	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/7);  total (13/7)

TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG  >  NC_000913/3941518‑3941620              |                                                                                          tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa            >  1:1774349/1‑93 (MQ=14) tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa            >  1:3670603/1‑93 (MQ=14)  gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCCGCGCCTGACTCAGTAt           >  1:1885859/1‑93 (MQ=11)  gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt           >  1:2045471/1‑93 (MQ=17)  gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt           >  2:4744815/1‑93 (MQ=17)   aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg          <  1:3020061/93‑1 (MQ=18)    agcATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt         <  1:1332675/91‑1 (MQ=16)    aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGTGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt         <  1:2851244/93‑1 (MQ=16)    aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt         <  2:2923038/93‑1 (MQ=18)    aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgg         >  1:3547838/1‑92 (MQ=16)     aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg        >  2:4444961/1‑93 (MQ=18)      cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt       <  1:4472268/93‑1 (MQ=21)       aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg      >  2:1604484/1‑93 (MQ=18)       aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg      <  2:69345/93‑1 (MQ=21)        tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt     >  2:2534427/1‑93 (MQ=18)        tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgggtgt     >  2:4449614/1‑93 (MQ=255)         cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt    >  1:3358051/1‑93 (MQ=18)          tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa   <  1:4615906/93‑1 (MQ=21)          tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa   >  2:4730900/1‑93 (MQ=18)          tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa   >  1:2660430/1‑93 (MQ=18)           aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg  <  2:2467380/93‑1 (MQ=21)              |                                                                                          TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG  >  NC_000913/3941518‑3941620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑