BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,182	T→C	I280T (ATC→ACC)	htpG →	chaperone Hsp90

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,182	0	T	C	98.8%	528.1 / ‑2.5	166	I280T (ATC→ACC)	htpG	chaperone Hsp90
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (83/81); total (85/81)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 178 total aligned reads displayed.

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

aCAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAccc                                                                                         >  2:1075244/1‑93 (MQ=255)

 cAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAcccc                                                                                        <  1:2811333/93‑1 (MQ=255)

  aaaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCg                                                                                       >  1:2545609/1‑93 (MQ=255)

   aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGt                                                                                      >  2:2547548/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTccc                                                                                   >  1:4104765/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  1:992195/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  1:4048662/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  2:550770/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   <  2:4582310/93‑1 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  1:2571865/1‑93 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  2:3064655/1‑93 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 <  2:2312184/93‑1 (MQ=255)

        aTCGCCCACGACTTTAATGATCCACTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 >  1:2223077/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTAGACCCCGTCCCAGGCt                                                                              >  1:1016374/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  1:1016373/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  1:2502623/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  2:3404231/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  1:4377517/1‑93 (MQ=255)

            cccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTc                                                                             >  1:2378474/1‑93 (MQ=255)

            cccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTc                                                                             >  1:2858656/1‑93 (MQ=255)

            cccACGACTCTAATGATCCGCTGACCTGGAGCCTCAACCGTGTTGAAGGCAAGCAGGAGGACACCAGCCTGCTGTACACCCCGTCCCAGGCTc                                                                             >  2:1237223/1‑93 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            >  1:828116/1‑93 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  1:486733/93‑1 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  1:3929520/93‑1 (MQ=255)

               aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGt                                                                          >  1:3508693/1‑93 (MQ=255)

               aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGt                                                                          >  2:1667878/1‑93 (MQ=255)

               aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGt                                                                          <  1:38905/93‑1 (MQ=255)

               aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGt                                                                          >  2:1667879/1‑93 (MQ=255)

                 gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTgg                                                                        <  1:2196688/93‑1 (MQ=255)

                 gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTgg                                                                        <  1:174363/93‑1 (MQ=255)

                  aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTggg                                                                       >  2:844605/1‑93 (MQ=255)

                   cTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTATACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGa                                                                      <  1:3345011/93‑1 (MQ=255)

                   cTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGa                                                                      <  2:2438589/93‑1 (MQ=255)

                   cTTTAATGATCCGCTGACCGGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACTCCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGa                                                                      <  1:673700/93‑1 (MQ=255)

                    tttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGat                                                                     >  2:124972/1‑93 (MQ=255)

                    tttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGat                                                                     >  2:302053/1‑93 (MQ=255)

                      tAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGatat                                                                   <  1:840961/93‑1 (MQ=255)

                       aaTGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATAtg                                                                  >  2:2492509/1‑93 (MQ=255)

                       aaTGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATAtg                                                                  <  1:1263814/93‑1 (MQ=255)

                        aTGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATAtgt                                                                 >  1:2088868/1‑93 (MQ=255)

                          gATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTgg                                                               <  1:3351081/93‑1 (MQ=255)

                          gATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTgg                                                               >  1:1292788/1‑93 (MQ=255)

                          gATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTgg                                                               >  2:335432/1‑93 (MQ=255)

                           aTCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGa                                                              >  1:103467/1‑93 (MQ=255)

                            tCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGaa                                                             >  2:4301105/1‑93 (MQ=255)

                               gCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcg                                                          <  1:3948250/93‑1 (MQ=255)

                               gCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGACTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcg                                                          >  2:3139754/1‑93 (MQ=255)

                                cTGACCTGGAGCCACAACCGAGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGTTCCATCGGATATGTGGAAcaca                                                         >  1:3171421/1‑90 (MQ=255)

                                  gACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACCCCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGa                                                       <  1:894708/93‑1 (MQ=255)

                                  gACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGa                                                       <  1:4625504/93‑1 (MQ=255)

                                  gACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGa                                                       >  1:2059512/1‑93 (MQ=255)

                                  gACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGa                                                       <  2:1948466/93‑1 (MQ=255)

                                     cTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCa                                                    >  2:3610173/1‑93 (MQ=255)

                                     cTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCa                                                    >  1:263884/1‑93 (MQ=255)

                                     cTGGAGCCACAACCGTCTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGTGATATGTGGAACCGCGATCa                                                    <  2:2292742/93‑1 (MQ=255)

                                       ggAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa                                                  >  1:635816/1‑93 (MQ=255)

                                       ggAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa                                                  >  2:4244242/1‑93 (MQ=255)

                                          gCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAac                                               <  2:957524/93‑1 (MQ=255)

                                          accACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCAGTACGCCCCGACCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAac                                               <  2:4334229/92‑1 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             >  1:1438640/1‑93 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            <  1:2017216/93‑1 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            <  2:1525140/93‑1 (MQ=255)

                                             acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACg                                            >  2:213928/1‑93 (MQ=255)

                                              caACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACgc                                           >  2:3257983/1‑92 (MQ=255)

                                               aaCCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGTTATGTGGAACCGCGATCATAAACACGGc                                          >  1:4175848/1‑93 (MQ=255)

                                                 ccGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCt                                        <  2:1757065/93‑1 (MQ=255)

                                                   gtgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGa                                      >  2:4478802/1‑93 (MQ=255)

                                                     gtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGaaa                                    >  1:2700406/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATACACACAGCCTGAAAc                                   >  2:1980404/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   <  2:282768/93‑1 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   >  2:2011651/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   <  2:857027/93‑1 (MQ=255)

                                                          aGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTa                               <  1:294703/93‑1 (MQ=255)

                                                          aGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTa                               <  2:1880632/93‑1 (MQ=255)

                                                          aGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTa                               <  2:1576073/93‑1 (MQ=255)

                                                             tAAGCAGGATTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGt                            <  1:128771/93‑1 (MQ=255)

                                                                gCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCa                         <  1:3742409/93‑1 (MQ=255)

                                                                gCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGACTGAAACTGTATGTTCa                         >  1:736742/1‑93 (MQ=255)

                                                                 cAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAg                        >  1:2498855/1‑93 (MQ=255)

                                                                 cAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAg                        <  2:2791166/93‑1 (MQ=255)

                                                                  aGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGc                       <  1:2834248/93‑1 (MQ=255)

                                                                  aGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGc                       >  1:2995206/1‑93 (MQ=255)

                                                                     aGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtg                    <  2:2264068/93‑1 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   <  1:3149810/93‑1 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   <  2:891306/93‑1 (MQ=255)

                                                                       tACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtg                  <  2:3943108/93‑1 (MQ=255)

                                                                       tACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtg                  <  2:3943109/93‑1 (MQ=255)

                                                                        acacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtgt                 <  2:871166/93‑1 (MQ=255)

                                                                         cacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGtt                >  1:2184747/1‑93 (MQ=255)

                                                                         cacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGtt                <  2:2241190/93‑1 (MQ=255)

                                                                         cacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGtt                <  1:1619315/93‑1 (MQ=255)

                                                                         cacCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGtt                >  2:4016947/1‑93 (MQ=255)

                                                                           tcAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGGGTGTtca              >  2:2949856/2‑93 (MQ=255)

                                                                              gCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTTGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatca           <  1:178524/93‑1 (MQ=255)

                                                                               ccTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTcatcat          <  1:4194742/93‑1 (MQ=255)

                                                                                ctgctgTACCCCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         <  2:1301783/93‑1 (MQ=255)

                                                                                ctgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         >  2:3787503/1‑93 (MQ=255)

                                                                                ctgctgCACTCCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCAGAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         <  1:2726758/93‑1 (MQ=255)

                                                                                  gctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGga       <  2:193903/93‑1 (MQ=255)

                                                                                      tACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGgacgac   <  1:973473/93‑1 (MQ=255)

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`