Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,962,662 A→G M195T (ATG→ACG)  rhlB ← putative ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,962,6620AG96.9% 489.7 / 4.0 161M195T (ATG→ACG) rhlBputative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base G (55/101);  total (55/106)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01

Only 100 of 171 total aligned reads displayed.

GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTT  >  NC_000913/3962573‑3962749
                                                                                         |                                                                                       
gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                      <  1:4324382/93‑1 (MQ=255)
  tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                    >  1:102050/1‑93 (MQ=255)
   ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                   <  1:590980/93‑1 (MQ=255)
   ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                   <  2:2606366/93‑1 (MQ=255)
   ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                   <  2:1246934/93‑1 (MQ=255)
   ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                   <  2:2217349/93‑1 (MQ=255)
   ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                   <  2:1637935/93‑1 (MQ=255)
    cGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAg                                                                                  <  2:457027/93‑1 (MQ=255)
     ggTTCCCCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                 <  2:4500924/93‑1 (MQ=255)
     ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                 <  1:948823/93‑1 (MQ=255)
     ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                 <  2:1562068/93‑1 (MQ=255)
      gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc                                                                                <  1:4020310/93‑1 (MQ=255)
       ttCCCCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                               >  1:3212518/1‑93 (MQ=255)
       ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                               >  1:3881494/1‑93 (MQ=255)
        tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgcgc                                                                              <  2:1582783/93‑1 (MQ=255)
         ccACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCt                                                                             >  2:3029386/1‑93 (MQ=255)
           aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                           >  2:1886443/1‑93 (MQ=255)
           aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                           >  1:2894347/1‑93 (MQ=255)
            cTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGATAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGt                                                                          <  2:2974209/93‑1 (MQ=255)
            cTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGt                                                                          >  2:1022406/1‑93 (MQ=255)
              tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGttt                                                                        >  1:2638082/1‑93 (MQ=255)
              tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGttt                                                                        <  1:4235691/93‑1 (MQ=255)
                aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                      <  2:69541/93‑1 (MQ=255)
                aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                      <  1:4308955/93‑1 (MQ=255)
                  tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                    <  2:2093581/93‑1 (MQ=255)
                  tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                    >  1:2172858/1‑93 (MQ=255)
                   atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                   <  2:975033/93‑1 (MQ=255)
                   atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                   >  1:1533716/1‑93 (MQ=255)
                    tatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggc                                                                  <  1:4338028/93‑1 (MQ=255)
                      tgcGGCATGGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcgg                                                                <  2:3163151/91‑1 (MQ=255)
                       tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGCGTTGAGGCGCTGGTTTGCAggcggc                                                               >  2:3402931/1‑93 (MQ=255)
                        cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                              >  2:1051759/1‑93 (MQ=255)
                        cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                              <  1:3711271/93‑1 (MQ=255)
                          gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                            <  1:2492129/93‑1 (MQ=255)
                          gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                            <  2:1628063/93‑1 (MQ=255)
                           cATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAc                                                           <  1:183159/93‑1 (MQ=255)
                            aTTGTTCATCTGCTCGAACGCCAGTTCAAGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGCGTTGAGGCGCTGGTTTGCAGGCGGAAAAcg                                                          >  2:1144871/1‑93 (MQ=255)
                             ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcgg                                                         <  1:1322880/93‑1 (MQ=255)
                             ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcgg                                                         <  2:3895132/93‑1 (MQ=255)
                              tGTTCATCTGCTCGAACGCCAGTTCACGTCCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                        <  2:3281249/93‑1 (MQ=255)
                              tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                        <  1:259491/93‑1 (MQ=255)
                              tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                        <  1:712948/93‑1 (MQ=255)
                              tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                        <  1:2002475/93‑1 (MQ=255)
                               gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                       >  1:518497/1‑93 (MQ=255)
                               gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                       <  2:1553371/93‑1 (MQ=255)
                                 tCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgta                                                     >  2:3908550/1‑91 (MQ=255)
                                 tCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGa                                                     <  2:2502382/93‑1 (MQ=255)
                                   aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                   <  2:3944276/93‑1 (MQ=255)
                                    tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                  <  1:2136400/93‑1 (MQ=255)
                                     cTGCTCGAGCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcag                                                 <  2:940757/93‑1 (MQ=255)
                                     cTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcag                                                 <  1:3247244/93‑1 (MQ=255)
                                       gCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcc                                               <  1:1333654/93‑1 (MQ=255)
                                       gCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcc                                               <  1:2264970/93‑1 (MQ=255)
                                       gCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcc                                               <  2:1450294/93‑1 (MQ=255)
                                         tCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccag                                             <  1:3842594/93‑1 (MQ=255)
                                          cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccagc                                            <  2:1660752/93‑1 (MQ=255)
                                          cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccagc                                            >  2:3680249/1‑93 (MQ=255)
                                           gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                           >  2:2938832/1‑93 (MQ=255)
                                           gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                           <  2:3165100/93‑1 (MQ=255)
                                            aaCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                          >  2:2558529/1‑93 (MQ=255)
                                             aCGCCAGTTCACGTACCCGGTACGAAAGCGTTGCGGAGAACATCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                         <  2:2024318/93‑1 (MQ=255)
                                             aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                         <  1:1808331/93‑1 (MQ=255)
                                                 cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGAtctc                                     >  1:3900893/1‑93 (MQ=255)
                                                 cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                     <  2:1164428/93‑1 (MQ=255)
                                                  aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCt                                    >  1:2972462/1‑93 (MQ=255)
                                                    ttCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCttt                                  <  1:2424059/93‑1 (MQ=255)
                                                     tCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTta                                 <  1:1250736/93‑1 (MQ=255)
                                                       aCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                               <  2:3129758/93‑1 (MQ=255)
                                                       aCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                               <  1:1045068/93‑1 (MQ=255)
                                                           aCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAg                           <  2:469398/93‑1 (MQ=255)
                                                           aCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAg                           >  1:2357044/1‑93 (MQ=255)
                                                            cccGGTACGAAAGCGTGGCGGTGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGc                          >  1:4716335/1‑93 (MQ=255)
                                                            cccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGc                          >  2:3032259/1‑93 (MQ=255)
                                                             ccGGTACGAAAGCGTGGCGGAGGACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                         >  1:1760938/1‑93 (MQ=255)
                                                              cGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGccc                        <  2:4719398/93‑1 (MQ=255)
                                                               ggTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                       <  1:4117419/93‑1 (MQ=255)
                                                               ggTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                       <  1:2330208/93‑1 (MQ=255)
                                                                 tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                     >  1:3317698/1‑93 (MQ=255)
                                                                 tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                     <  2:3343160/93‑1 (MQ=255)
                                                                 tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                     <  2:2786363/93‑1 (MQ=255)
                                                                  tctaaaGCGTTTCTGAGAACAGCGTGTTGAGGCGCTGGTTGTCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                    <  1:2813985/90‑1 (MQ=255)
                                                                   cGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                   >  2:550872/1‑93 (MQ=255)
                                                                    gAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCg                  >  2:4230628/1‑93 (MQ=255)
                                                                       aGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTAc               <  1:966904/93‑1 (MQ=255)
                                                                        gCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa              <  1:379409/93‑1 (MQ=255)
                                                                        gCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa              <  1:679841/93‑1 (MQ=255)
                                                                        gCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGACCAGATCGTACa              >  2:4408792/1‑93 (MQ=255)
                                                                        gCGGGGCGGAGAACAGCGGGGGGAGGCGCCGGTGGGCAGGCGGCAGACGGCGGAACAGCCAGCGGATAGCGTCAAGAAAGCCCAGACCGcaca              >  1:3618876/1‑93 (MQ=255)
                                                                         cttGGCGGAAAACAGCGTGTTGAGGCGCTGCTTTGCAGTCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt             <  2:4636773/91‑1 (MQ=255)
                                                                         cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt             <  1:2731022/93‑1 (MQ=255)
                                                                           tgtcGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc           <  2:3162009/90‑1 (MQ=255)
                                                                           tGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc           <  2:2716703/93‑1 (MQ=255)
                                                                             gCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAGTCAAGCCCAGATCGTACATGCgt         >  2:2634288/1‑92 (MQ=255)
                                                                              cGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGAt        <  1:3068437/93‑1 (MQ=255)
                                                                               ggAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc       >  2:2858346/1‑93 (MQ=255)
                                                                                gagaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCg      >  1:80287/1‑93 (MQ=255)
                                                                                gagaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCg      <  2:2437987/93‑1 (MQ=255)
                                                                                  gaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGc    <  2:2447474/93‑1 (MQ=255)
                                                                                  gaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGc    >  1:4166889/1‑93 (MQ=255)
                                                                                  gaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGc    >  1:4422691/1‑93 (MQ=255)
                                                                                         |                                                                                       
GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTT  >  NC_000913/3962573‑3962749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑