BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,651	A→G	L427L (TTA→TTG)	ybbX →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,651	0	A	G	98.1%	665.9 / 0.9	211	L427L (TTA→TTG)	ybbX	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (114/93); total (116/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 216 total aligned reads displayed.

GTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCC  >  NC_000913/539562‑539739

gTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgt                                                                                       <  1:1263185/93‑1 (MQ=255)

 ttCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGGATCACGAAAACTATCTTTCgcg                                                                                      >  1:3525657/1‑91 (MQ=255)

   cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggt                                                                                    <  1:4712337/93‑1 (MQ=255)

   cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggt                                                                                    <  2:2110195/93‑1 (MQ=255)

   cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACgtggt                                                                                    >  2:3680416/1‑93 (MQ=255)

      aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                 <  2:1446368/93‑1 (MQ=255)

       ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                >  1:2012064/1‑93 (MQ=255)

       ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                <  2:3513858/93‑1 (MQ=255)

        cAATGACGATCTGGAATATCGCCACAAAGTCCGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgt                                                                               >  1:1104792/1‑93 (MQ=255)

        cAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgt                                                                               <  1:3499553/93‑1 (MQ=255)

         aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                              >  2:3863809/1‑93 (MQ=255)

         aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                              >  2:4588458/1‑93 (MQ=255)

         aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                              <  2:689201/93‑1 (MQ=255)

         aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                              >  1:1199479/1‑93 (MQ=255)

           tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                            <  2:4047882/93‑1 (MQ=255)

           tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                            <  1:2830248/93‑1 (MQ=255)

           tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                            <  2:2947475/93‑1 (MQ=255)

            gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAtt                                                                           <  1:2181604/93‑1 (MQ=255)

                aTCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACg                                                                       >  2:369722/1‑93 (MQ=255)

                 tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                      >  2:4483751/1‑93 (MQ=255)

                 cttGGAATATCGCCACAAATTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCGTGCGTGGTGATGTGATTTACGa                                                                      >  1:4206497/3‑93 (MQ=255)

                  cTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGAc                                                                     >  1:1908788/1‑93 (MQ=255)

                    ggcatatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCGCGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                   <  1:4252284/90‑1 (MQ=255)

                     gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                  >  2:3621641/1‑93 (MQ=255)

                      gatatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTg                                                                 <  1:2749400/92‑1 (MQ=255)

                       atatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGa                                                                <  2:3231710/93‑1 (MQ=255)

                       atatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTAAGACAttta                                                                >  2:3420497/1‑91 (MQ=255)

                         atCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                              >  2:2296742/1‑93 (MQ=255)

                          tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCTCGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAcg                                                             >  1:2151845/1‑92 (MQ=255)

                          tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACa                                                             >  1:3656992/1‑93 (MQ=255)

                          acGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACa                                                             <  2:1592669/92‑1 (MQ=255)

                            gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAgg                                                           <  1:1156958/93‑1 (MQ=255)

                            gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAgg                                                           <  2:2861366/93‑1 (MQ=255)

                              cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                         >  1:2971968/1‑93 (MQ=255)

                              cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                         <  1:3330352/93‑1 (MQ=255)

                              cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                         >  2:2586277/1‑93 (MQ=255)

                                caAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCtt                                                       >  2:4416078/1‑93 (MQ=255)

                                caAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCtt                                                       <  1:4233168/93‑1 (MQ=255)

                                 aaaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTc                                                      >  1:2028249/1‑93 (MQ=255)

                                  aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                     >  2:265242/1‑93 (MQ=255)

                                  aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                     >  1:4540703/1‑93 (MQ=255)

                                  aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                     >  1:4633998/1‑93 (MQ=255)

                                   aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTacc                                                    >  1:2364772/1‑93 (MQ=255)

                                    gTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCt                                                   <  2:4022948/93‑1 (MQ=255)

                                    gTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCt                                                   <  1:1357466/93‑1 (MQ=255)

                                     tCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTg                                                  >  1:880049/1‑93 (MQ=255)

                                     tCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTg                                                  >  1:2506237/1‑93 (MQ=255)

                                     tCAGACCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAAGGCTTCCCTg                                                  <  2:4195234/93‑1 (MQ=255)

                                      cAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGt                                                 <  1:2460832/93‑1 (MQ=255)

                                       aGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGtt                                                >  2:724009/1‑93 (MQ=255)

                                           cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                            >  1:775928/1‑93 (MQ=255)

                                             tATGTTGGCCGTACTATTGGCGCGCGTATCACGAAAACCAACTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGCTGCGCCg                                          >  1:362859/1‑93 (MQ=255)

                                             tATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCg                                          >  1:3994213/1‑93 (MQ=255)

                                             tATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCg                                          >  1:3229351/1‑93 (MQ=255)

                                               tGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaa                                        >  1:63542/1‑93 (MQ=255)

                                               tGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaa                                        >  2:2781359/1‑93 (MQ=255)

                                                gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                       >  1:1876842/1‑93 (MQ=255)

                                                gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                       <  2:2175002/93‑1 (MQ=255)

                                                    gCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTc                                   <  2:2986534/93‑1 (MQ=255)

                                                     gcGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCa                                  <  2:1120102/92‑1 (MQ=255)

                                                      cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                 <  1:1828532/93‑1 (MQ=255)

                                                      cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                 <  2:4281068/93‑1 (MQ=255)

                                                       gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                >  2:3333128/1‑93 (MQ=255)

                                                        tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                               >  1:1552882/1‑93 (MQ=255)

                                                            aTTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATc                           <  1:2440912/93‑1 (MQ=255)

                                                            aTTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATc                           >  1:1033606/1‑93 (MQ=255)

                                                             ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                          >  2:1940692/1‑93 (MQ=255)

                                                             ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                          >  1:1323331/1‑93 (MQ=255)

                                                             ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                          >  2:1036564/1‑93 (MQ=255)

                                                             ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                          <  1:3263880/93‑1 (MQ=255)

                                                               ggCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCta                        >  1:1615496/1‑92 (MQ=255)

                                                                gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                       <  1:4303315/93‑1 (MQ=255)

                                                                  gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                     >  2:2386487/1‑93 (MQ=255)

                                                                  gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                     <  2:3452024/93‑1 (MQ=255)

                                                                   cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                    <  2:4199254/93‑1 (MQ=255)

                                                                   cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                    <  2:2468741/93‑1 (MQ=255)

                                                                   cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                    <  2:1924739/93‑1 (MQ=255)

                                                                    gcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACa                   <  1:1496104/93‑1 (MQ=255)

                                                                    gcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACa                   >  1:1707094/1‑93 (MQ=255)

                                                                     cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                  <  2:2741215/93‑1 (MQ=255)

                                                                      gTATCACGAAAACCATCTTGCGCGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                 <  2:395461/93‑1 (MQ=255)

                                                                       tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                <  1:4070648/93‑1 (MQ=255)

                                                                       tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                >  2:3160056/1‑93 (MQ=255)

                                                                       tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                >  1:2889024/1‑93 (MQ=255)

                                                                       tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                <  1:3676265/93‑1 (MQ=255)

                                                                        aTCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcag               >  1:4482536/1‑93 (MQ=255)

                                                                          cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca             <  1:262865/93‑1 (MQ=255)

                                                                          cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca             <  1:2923307/93‑1 (MQ=255)

                                                                           aCGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagcag            <  2:644993/93‑1 (MQ=255)

                                                                            cGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGt           <  2:770529/93‑1 (MQ=255)

                                                                             gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa          >  1:1645886/1‑93 (MQ=255)

                                                                             gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa          <  1:212100/93‑1 (MQ=255)

                                                                               aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt        >  1:3752107/1‑93 (MQ=255)

                                                                                aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc       >  2:4650728/1‑93 (MQ=255)

                                                                                aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc       >  2:528071/1‑93 (MQ=255)

                                                                                  acATCTTGCGTGGTGATGTTATTTACGGCATTTAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg     <  1:3972814/92‑1 (MQ=255)

                                                                                    aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc   >  2:1291433/1‑93 (MQ=255)

                                                                                    aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc   >  1:1313116/1‑93 (MQ=255)

                                                                                    aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc   >  1:4030527/1‑93 (MQ=255)

                                                                                     tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc  <  1:841175/93‑1 (MQ=255)

GTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCC  >  NC_000913/539562‑539739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`