BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,390	C→T	A65V (GCG→GTG)	hemG →	protoporphyrin oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,390	0	C	T	98.2%	591.4 / ‑2.4	171	A65V (GCG→GTG)	hemG	protoporphyrin oxidase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (84/84); total (86/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 177 total aligned reads displayed.

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCG  >  NC_000913/4032300‑4032478

ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                                                                                        <  1:1072553/93‑1 (MQ=255)

ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                                                                                        <  1:1194688/93‑1 (MQ=255)

ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                                                                                        >  1:4285978/1‑93 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                      >  2:2159029/1‑93 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                      >  2:3326790/1‑93 (MQ=255)

   aTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTcc                                                                                     >  2:3829863/1‑93 (MQ=255)

   aTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTcc                                                                                     <  2:3787158/93‑1 (MQ=255)

    tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                                                                                    >  1:1752156/1‑93 (MQ=255)

    tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                                                                                    <  1:2626679/93‑1 (MQ=255)

    tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                                                                                    >  1:2255552/1‑93 (MQ=255)

     gtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAg                                                                                   <  2:3852301/93‑1 (MQ=255)

      tgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGTTCACTACCATTCAGTGTTCCAgg                                                                                  >  2:2606050/1‑93 (MQ=255)

      tgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAgg                                                                                  <  2:469506/93‑1 (MQ=255)

        cACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGaa                                                                                <  2:1146456/93‑1 (MQ=255)

        cACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGaa                                                                                >  1:2613321/1‑93 (MQ=255)

         aCCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAt                                                                               >  1:3939494/1‑93 (MQ=255)

          ccGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAtt                                                                              >  1:2531359/1‑93 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                            >  2:3365922/1‑93 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                            >  1:2889574/1‑93 (MQ=255)

              aTTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTc                                                                          >  2:1106569/1‑93 (MQ=255)

              aTTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTc                                                                          >  2:4730426/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                         >  1:463125/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                         >  2:2547797/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                         >  1:1503589/1‑93 (MQ=255)

                 gaagaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaa                                                                       >  1:923618/1‑93 (MQ=255)

                   agaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaa                                                                     <  2:64596/93‑1 (MQ=255)

                     aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAc                                                                   <  2:78518/93‑1 (MQ=255)

                     aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAc                                                                   >  1:419039/1‑93 (MQ=255)

                      aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa                                                                  >  1:164003/1‑93 (MQ=255)

                      aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa                                                                  <  1:3855768/93‑1 (MQ=255)

                      aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa                                                                  >  2:466705/1‑93 (MQ=255)

                       ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt                                                                 <  1:755094/93‑1 (MQ=255)

                        cacgGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATg                                                                <  2:3211613/93‑1 (MQ=255)

                         ccggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGc                                                               <  2:1510149/90‑1 (MQ=255)

                          cggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCg                                                              <  2:4422141/91‑1 (MQ=255)

                           ggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGa                                                             <  2:544597/92‑1 (MQ=255)

                            gTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                            <  1:1921611/93‑1 (MQ=255)

                            gTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATACGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                            <  1:4620636/93‑1 (MQ=255)

                              gggAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcgc                                                          <  2:2796945/93‑1 (MQ=255)

                               ggAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACCACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcgcg                                                         <  1:1667035/93‑1 (MQ=255)

                                 aaaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGc                                                       >  1:442874/1‑93 (MQ=255)

                                   aaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTg                                                     <  1:4137995/93‑1 (MQ=255)

                                    aCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGa                                                    >  1:573880/1‑93 (MQ=255)

                                     cTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCTCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                   <  1:1023719/93‑1 (MQ=255)

                                     cTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                   <  2:2530262/93‑1 (MQ=255)

                                      tATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAt                                                  >  1:1320507/1‑93 (MQ=255)

                                        tGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTc                                                >  1:2572007/1‑93 (MQ=255)

                                          gccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                              <  2:1508068/92‑1 (MQ=255)

                                          aCCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                              <  2:1862079/93‑1 (MQ=255)

                                           ccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGAt                                             >  1:2496371/1‑93 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                           <  1:2683120/93‑1 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                        >  1:303082/1‑93 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                        >  2:1582173/1‑93 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                        <  1:2748579/93‑1 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                        <  1:4369165/93‑1 (MQ=255)

                                                 ggTTATTAGTGCTCCTATTCGCTATGATCACTACCATTCAGTTTTCCAGAAATTTGTAAAAAAACATGCGACGCGGCTGAATTCGATGCCGAg                                       <  2:4402574/93‑1 (MQ=255)

                                                 ggTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAg                                       <  2:397938/93‑1 (MQ=255)

                                                 ggTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAg                                       <  2:4486488/93‑1 (MQ=255)

                                                   tCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAgcg                                     >  2:1650113/1‑93 (MQ=255)

                                                     aTTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGcc                                   <  2:1559023/93‑1 (MQ=255)

                                                      ttGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCt                                  <  2:4293144/93‑1 (MQ=255)

                                                      ttGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCt                                  >  2:4117875/1‑93 (MQ=255)

                                                       tGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCtt                                 >  1:2372189/1‑93 (MQ=255)

                                                        ggTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCttt                                <  2:1866294/93‑1 (MQ=255)

                                                        ggTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCttt                                <  1:1305967/93‑1 (MQ=255)

                                                           gCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTAc                             <  1:325914/93‑1 (MQ=255)

                                                             ttCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTc                           <  1:4496156/93‑1 (MQ=255)

                                                               cTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCg                         <  1:2212687/93‑1 (MQ=255)

                                                               cTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCg                         >  1:641585/1‑93 (MQ=255)

                                                                tATTCGCTATGGTCACTACCAGTCAGTGGTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                        <  2:85814/93‑1 (MQ=255)

                                                                 aTTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTg                       <  1:1815661/93‑1 (MQ=255)

                                                                  ttCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGa                      >  2:919435/1‑93 (MQ=255)

                                                                  ttCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGa                      <  1:2534602/93‑1 (MQ=255)

                                                                  ttCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGa                      >  2:2745535/1‑93 (MQ=255)

                                                                   tCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGaa                     <  2:2718450/93‑1 (MQ=255)

                                                                     gCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATc                   <  1:4106568/93‑1 (MQ=255)

                                                                      cTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCt                  <  1:1369764/93‑1 (MQ=255)

                                                                       tATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtg                 <  2:393653/93‑1 (MQ=255)

                                                                        aTGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAACGCCTTTTACTCCGTGAATCtgg                >  2:2634665/1‑93 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt               <  2:425004/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt               >  2:412296/1‑93 (MQ=255)

                                                                          ggTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggtg              <  1:4455490/93‑1 (MQ=255)

                                                                             cACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcg           >  2:1555413/1‑93 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc          <  2:2054693/93‑1 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc          <  2:558043/93‑1 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc          >  2:2300556/1‑93 (MQ=255)

                                                                               cTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcg         >  1:254715/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa       >  1:78123/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa       <  2:3428736/93‑1 (MQ=255)

                                                                                 aCCATTAAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa       >  2:4608790/1‑93 (MQ=255)

                                                                                  ccATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgcga      >  2:1606668/1‑91 (MQ=255)

                                                                                   cATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaaa     <  2:3675284/93‑1 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc    <  1:3729376/93‑1 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc    >  1:3196285/1‑93 (MQ=255)

                                                                                    aTTCAATGTTCCAGGAATGTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGGGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc    <  1:1522862/93‑1 (MQ=255)

                                                                                      tCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg  <  1:360437/93‑1 (MQ=255)

                                                                                      tCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg  <  1:3394023/93‑1 (MQ=255)

                                                                                      tCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg  <  1:3035470/93‑1 (MQ=255)

                                                                                      tCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg  >  2:1628726/1‑93 (MQ=255)

                                                                                      tCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCg  <  2:4739292/93‑1 (MQ=255)

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCG  >  NC_000913/4032300‑4032478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`