BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,827	A→G	Q78R (CAG→CGG)	yihZ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,827	0	A	G	98.4%	396.3 / ‑3.0	128	Q78R (CAG→CGG)	yihZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (58/68); total (60/68)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 141 total aligned reads displayed.

GTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACT  >  NC_000913/4074737‑4074916

gtgtGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGt                                                                                         <  2:4649627/93‑1 (MQ=255)

gtgtGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGt                                                                                         <  1:4164822/93‑1 (MQ=255)

 tgtgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGtt                                                                                        >  1:4595856/1‑93 (MQ=255)

 tgtgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGtt                                                                                        <  2:4461114/93‑1 (MQ=255)

 tgtgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGCGTGCTGGTGGTTTTCCGGtt                                                                                        >  2:2771643/1‑93 (MQ=255)

  gtgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGttt                                                                                       <  2:846234/93‑1 (MQ=255)

       cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCt                                                                                  >  1:490012/1‑93 (MQ=255)

       cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCt                                                                                  <  2:2226698/93‑1 (MQ=255)

       cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCt                                                                                  >  1:2289573/1‑93 (MQ=255)

        ggCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTc                                                                                 <  1:256192/93‑1 (MQ=255)

        ggCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTc                                                                                 <  2:2524990/93‑1 (MQ=255)

          gtACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTcgc                                                                               <  2:1115771/92‑1 (MQ=255)

           tACCGCATCTTTAGCGATGCCTAGGGGAAGATGAAGCTCAACATGCAACAGGCGGGCCGGAGCGTCCTGGTGGGTTCCGGGGTTACCGTcccg                                                                              >  2:72310/1‑90 (MQ=255)

           tACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTcgcc                                                                              <  2:2526395/93‑1 (MQ=255)

           tACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGAGTGCTGGTGGTTTCCCGGTTTACCCTctcc                                                                              >  2:3880899/1‑93 (MQ=255)

            aCCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTcgccg                                                                             >  1:401022/1‑93 (MQ=255)

             ccGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTcgccgc                                                                            >  2:4063542/1‑93 (MQ=255)

              cGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCa                                                                           <  2:2692060/93‑1 (MQ=255)

                cATCTTTAGCGATGCGGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGa                                                                         <  2:350645/93‑1 (MQ=255)

                cATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGa                                                                         <  2:350646/93‑1 (MQ=255)

                 aTCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGAt                                                                        >  2:3526458/1‑93 (MQ=255)

                 aTCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGAt                                                                        >  2:3214640/1‑93 (MQ=255)

                  tCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGTGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGAtc                                                                       >  1:2698379/1‑92 (MQ=255)

                  tCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCCCCGCCGATa                                                                       >  1:1153838/1‑93 (MQ=255)

                   cTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATAc                                                                      <  2:3467430/93‑1 (MQ=255)

                    tttAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATAcc                                                                     >  2:1824015/1‑93 (MQ=255)

                     ttAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCg                                                                    >  1:1680526/1‑93 (MQ=255)

                       aGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGaa                                                                  <  2:3412806/93‑1 (MQ=255)

                       aGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGaa                                                                  >  2:4717465/1‑93 (MQ=255)

                       aGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGaa                                                                  >  2:1275952/1‑93 (MQ=255)

                        gCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAAc                                                                 >  2:3041400/1‑93 (MQ=255)

                        gCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAAc                                                                 <  2:2660483/93‑1 (MQ=255)

                          gATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACgg                                                               >  1:639497/1‑93 (MQ=255)

                          gATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACgg                                                               <  2:2951009/93‑1 (MQ=255)

                             gCCGAAGGCAAGATGAATCTGAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACggggg                                                            <  2:1222553/93‑1 (MQ=255)

                             gCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGGACggggg                                                            >  1:2326735/1‑93 (MQ=255)

                             gCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACggggg                                                            <  1:2432108/93‑1 (MQ=255)

                             gCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACggggg                                                            >  2:4002294/1‑93 (MQ=255)

                              ccGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGa                                                           <  1:3254576/93‑1 (MQ=255)

                              ccGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGa                                                           >  2:481897/1‑93 (MQ=255)

                              ccGAAGGCAAGATAAATCTCAGCTTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAGCGGGGGa                                                           <  2:3244368/93‑1 (MQ=255)

                               cGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGag                                                          >  2:3657746/1‑92 (MQ=255)

                                gAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATg                                                         <  1:789726/93‑1 (MQ=255)

                                 aaGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATgc                                                        <  2:467890/93‑1 (MQ=255)

                                 aaGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATgc                                                        >  2:958925/1‑93 (MQ=255)

                                 aaGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGAGCTGGTGGTTTCCTGGTTTAGCCTCGCCGCCGACACCGACCGGGGGATgc                                                        >  1:4606061/1‑93 (MQ=255)

                                   ggCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATgcgc                                                      >  2:1932606/1‑93 (MQ=255)

                                    gCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGcc                                                     >  1:2779447/1‑93 (MQ=255)

                                     cAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACAGAACGGGGGATGCGccc                                                    >  1:754970/1‑93 (MQ=255)

                                        gATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAg                                                 >  2:2153840/1‑93 (MQ=255)

                                          tGAATCTCAACGTGCAGCAGGCGGGCGGCAGTGTGCTGATGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGtt                                               <  1:2995174/93‑1 (MQ=255)

                                          tGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGtt                                               >  1:3622212/1‑93 (MQ=255)

                                          tGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGtt                                               <  1:2995054/93‑1 (MQ=255)

                                           gAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                              >  1:2799179/1‑93 (MQ=255)

                                            aaTCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGCGGTTTCCCGGTTTACCCTCGCCGCATATACCGATCGGGGCGTGCGCCCGAGGTtc                                             >  2:2289899/1‑93 (MQ=255)

                                             aTCTCAACGAGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGACGCAGATACCGAACGGGGGATGCGCCCACGTTtct                                            >  1:2510341/1‑93 (MQ=255)

                                              tctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtctc                                           <  1:4732005/93‑1 (MQ=255)

                                               ctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATCCCGAACGGGGGATGCGCCCAAGTTTCTcc                                          <  1:1770390/93‑1 (MQ=255)

                                               ctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTcc                                          <  1:1393572/93‑1 (MQ=255)

                                                  aaCGTGCAACAGGCGTGCGGCAGTTTTCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGTGGATGCGCCCAAGTTTCTCCaaa                                       <  1:1983219/93‑1 (MQ=255)

                                                  aaCGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaaa                                       <  1:1700867/93‑1 (MQ=255)

                                                     gtgtAACAGGCGGGCGGCAGTGTGCTGGTGGTTGCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGt                                    <  1:1384422/93‑1 (MQ=255)

                                                     gTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATCCCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGt                                    <  2:1077959/93‑1 (MQ=255)

                                                      tGCAACAGGCGGGCGGGAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTg                                   >  2:4509358/1‑93 (MQ=255)

                                                       gCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGc                                  <  1:1834346/93‑1 (MQ=255)

                                                        cAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCa                                 >  1:1005995/1‑93 (MQ=255)

                                                        cAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCa                                 <  1:3978826/93‑1 (MQ=255)

                                                        cAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCAAAGTTTCTCCAAAGGTGCa                                 <  2:2801365/93‑1 (MQ=255)

                                                         aaCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCAt                                >  2:4731377/1‑93 (MQ=255)

                                                         aaCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCAt                                <  1:1053203/93‑1 (MQ=255)

                                                          aCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATc                               >  2:409480/1‑93 (MQ=255)

                                                          aCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATc                               >  1:4746542/1‑93 (MQ=255)

                                                          aCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATc                               <  1:3861403/93‑1 (MQ=255)

                                                          aCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATc                               >  1:4485814/1‑93 (MQ=255)

                                                          aCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATc                               >  1:2048050/1‑93 (MQ=255)

                                                           cAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCACAGGGGCATCa                              >  2:38948/1‑93 (MQ=255)

                                                            aGGCGGGCGGCAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAc                             >  1:1587208/1‑93 (MQ=255)

                                                             ggcgggcgGCAGTGTGCTGGTGGTTTCCCGCTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAcc                            <  2:450483/93‑1 (MQ=255)

                                                                 ggcggcAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGAt                        >  1:396986/1‑93 (MQ=255)

                                                                  gcggcAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATc                       >  2:2931653/1‑93 (MQ=255)

                                                                  gcggcAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATc                       >  2:2931652/1‑93 (MQ=255)

                                                                     gcAGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcgcg                    >  2:2030235/1‑93 (MQ=255)

                                                                       aGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCa                  >  1:2633757/1‑93 (MQ=255)

                                                                       aGTGTGCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCa                  <  1:3795935/93‑1 (MQ=255)

                                                                         tgtgCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCaga                <  2:1878098/93‑1 (MQ=255)

                                                                         tgtgCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCaga                <  1:336965/93‑1 (MQ=255)

                                                                          gtgCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGTGGATGCGCCCCAGTTTCTCCAAAGGTGCATCACCGGATCGCGCagag               >  2:1366720/1‑93 (MQ=255)

                                                                          gtgCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCagag               <  1:3309340/93‑1 (MQ=255)

                                                                            gCTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGc             <  1:875978/93‑1 (MQ=255)

                                                                             cTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCg            <  2:4149868/93‑1 (MQ=255)

                                                                             cTGGTGGTTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCg            <  2:70022/93‑1 (MQ=255)

                                                                                 tggtTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTtat        <  2:32743/93‑1 (MQ=255)

                                                                                 tggtTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTACATCACCGGATCGCGCAGAGGCGTtat        <  2:2669467/93‑1 (MQ=255)

                                                                                  ggtTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTtata       <  2:3587116/93‑1 (MQ=255)

                                                                                  ggtTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTtata       <  1:4004111/93‑1 (MQ=255)

                                                                                   gtTTCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTatat      <  1:4234426/93‑1 (MQ=255)

                                                                                      tCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGAc   <  1:2005256/93‑1 (MQ=255)

                                                                                      tCCCGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGAc   >  1:4244689/1‑93 (MQ=255)

                                                                                       cccGGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACt  <  2:3166709/93‑1 (MQ=255)

                                                                                       cccAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCCCCGGATCGCGCAGAGGCGTTATATGACt  >  2:4004980/1‑93 (MQ=255)

GTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACT  >  NC_000913/4074737‑4074916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`