Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,130,739 A→G T182A (ACT→GCT)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,7390AG99.4% 567.5 / ‑4.9 176T182A (ACT→GCT) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (94/81);  total (95/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 181 total aligned reads displayed.

ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130650‑4130828
                                                                                         |                                                                                         
aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                        >  2:2892705/1‑93 (MQ=255)
 tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       >  2:625896/1‑93 (MQ=255)
  ccGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAg                                                                                      >  2:4243073/1‑93 (MQ=255)
   cGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGCGGATGCCGGAGACAACCGCGCGAATGCTCAGt                                                                                     >  1:2110247/1‑93 (MQ=255)
    ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    >  1:1087835/1‑93 (MQ=255)
    ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    >  2:439639/1‑93 (MQ=255)
    ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    >  1:3790556/1‑93 (MQ=255)
     gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                   <  1:257187/93‑1 (MQ=255)
     gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                   <  2:1735830/93‑1 (MQ=255)
      aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                  <  2:3611712/93‑1 (MQ=255)
        gTTCACCCGGAAGCACAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  2:2811765/93‑1 (MQ=255)
        gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                >  2:402313/1‑93 (MQ=255)
        gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  1:4041133/93‑1 (MQ=255)
          tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  2:3815130/93‑1 (MQ=255)
          tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              >  2:3903298/1‑93 (MQ=255)
           cACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttcttc                                                                             >  2:1092526/1‑93 (MQ=255)
           cACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttcttc                                                                             <  1:344472/93‑1 (MQ=255)
           cACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttcttc                                                                             >  2:2884162/1‑93 (MQ=255)
             cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           >  1:2007416/1‑93 (MQ=255)
             cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  2:3744059/93‑1 (MQ=255)
              ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTTATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                          >  2:3433990/1‑93 (MQ=255)
              ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                          <  2:3252733/93‑1 (MQ=255)
              ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                          <  1:1972647/93‑1 (MQ=255)
                ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                        >  1:3186497/1‑93 (MQ=255)
                 gAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTc                                                                       >  1:3006230/1‑93 (MQ=255)
                 gAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTc                                                                       >  1:3514403/1‑93 (MQ=255)
                    gCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaa                                                                    >  1:4249640/1‑93 (MQ=255)
                     cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   <  1:1464181/93‑1 (MQ=255)
                     cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   <  2:4592900/93‑1 (MQ=255)
                      aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  >  1:4458513/1‑93 (MQ=255)
                       aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 >  1:272114/1‑93 (MQ=255)
                          aGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAc                                                              >  2:2466584/1‑93 (MQ=255)
                           gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                             >  1:1154437/1‑93 (MQ=255)
                           gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                             <  2:2730189/93‑1 (MQ=255)
                           gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                             <  2:4179604/93‑1 (MQ=255)
                            cgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCt                                                            <  1:1790695/93‑1 (MQ=255)
                              cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          <  2:3075724/93‑1 (MQ=255)
                              cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          >  1:1554541/1‑93 (MQ=255)
                               tCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCg                                                         >  2:3781058/1‑93 (MQ=255)
                               tCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCg                                                         >  1:518982/1‑93 (MQ=255)
                                 aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       <  2:399324/93‑1 (MQ=255)
                                 aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       >  2:4250598/1‑93 (MQ=255)
                                 aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       <  1:3069772/93‑1 (MQ=255)
                                  ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                      >  1:4730149/1‑93 (MQ=255)
                                    cGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTc                                                    <  2:743394/93‑1 (MQ=255)
                                     ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   >  1:879527/1‑93 (MQ=255)
                                       aTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTg                                                 >  2:2278821/1‑93 (MQ=255)
                                        tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                <  1:1019266/93‑1 (MQ=255)
                                        tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                >  1:948303/1‑93 (MQ=255)
                                         ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc                                               <  2:1991076/93‑1 (MQ=255)
                                         ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc                                               <  2:1388737/93‑1 (MQ=255)
                                          tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              <  1:579481/93‑1 (MQ=255)
                                             ttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCt                                           >  2:3677523/1‑93 (MQ=255)
                                              tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  2:3508254/93‑1 (MQ=255)
                                              tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          >  1:2870416/1‑93 (MQ=255)
                                               aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         <  2:4487669/93‑1 (MQ=255)
                                               aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         >  1:2975338/1‑93 (MQ=255)
                                                 tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       <  2:2391611/93‑1 (MQ=255)
                                                 tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       <  1:2837037/93‑1 (MQ=255)
                                                 tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       <  1:3294843/93‑1 (MQ=255)
                                                 tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGAGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       >  2:412450/1‑93 (MQ=255)
                                                  cTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTa                                      <  2:258688/93‑1 (MQ=255)
                                                     aaaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcg                                   <  1:4637259/93‑1 (MQ=255)
                                                     aaaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcg                                   >  1:2271996/1‑93 (MQ=255)
                                                       aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                 >  2:1523197/1‑93 (MQ=255)
                                                        cGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcg                                <  1:4485268/93‑1 (MQ=255)
                                                        cGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcg                                >  1:3805022/1‑93 (MQ=255)
                                                         gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTGTCGTGACCGCTGTGTATcggcgg                               >  2:3378155/1‑93 (MQ=255)
                                                         ccAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                               <  2:4052811/92‑1 (MQ=255)
                                                          cAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCggg                              <  2:1198831/93‑1 (MQ=255)
                                                          cAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCggg                              >  1:3872512/1‑93 (MQ=255)
                                                           aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCgggg                             >  1:1723926/1‑92 (MQ=255)
                                                           aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGc                             >  1:2171830/1‑93 (MQ=255)
                                                            aaGTGGATGTCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCa                            >  2:766153/1‑93 (MQ=255)
                                                            aaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCgggga                            >  2:1060842/1‑91 (MQ=255)
                                                            aaGTGGATGCCGAAGCCAACCGCGCGATTGCTCAGGTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGcc                            >  2:253400/1‑92 (MQ=255)
                                                             aGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGcgt                           >  1:4159596/1‑91 (MQ=255)
                                                              gTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAtt                          <  1:1048850/93‑1 (MQ=255)
                                                                ggATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                        <  1:1173503/93‑1 (MQ=255)
                                                                 gATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTgtt                       >  1:4115815/1‑91 (MQ=255)
                                                                 gATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAt                       <  1:3795118/93‑1 (MQ=255)
                                                                 gATGCCGGAGCCAACCGCGCGAGTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAt                       <  2:2027191/93‑1 (MQ=255)
                                                                  aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtg                      <  2:3340316/93‑1 (MQ=255)
                                                                  aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtg                      <  2:108046/93‑1 (MQ=255)
                                                                   tGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtgt                     <  1:366707/93‑1 (MQ=255)
                                                                    gCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtgtg                    <  2:2983256/93‑1 (MQ=255)
                                                                      cGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGa                  <  1:346730/93‑1 (MQ=255)
                                                                        gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGgaa                >  2:880549/1‑93 (MQ=255)
                                                                             aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCTGTGATGTGGGAattatt           >  1:253739/1‑93 (MQ=255)
                                                                              aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          >  2:2332482/1‑93 (MQ=255)
                                                                              aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          <  1:2396832/93‑1 (MQ=255)
                                                                              aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          <  2:3185114/93‑1 (MQ=255)
                                                                               ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAattatttc         >  2:821182/1‑91 (MQ=255)
                                                                               ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc         <  2:1908872/93‑1 (MQ=255)
                                                                               ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc         <  1:1710063/93‑1 (MQ=255)
                                                                               ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc         <  2:2844597/93‑1 (MQ=255)
                                                                                 gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCgg       >  2:4702795/1‑93 (MQ=255)
                                                                                  cgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCggg      <  2:1268894/93‑1 (MQ=255)
                                                                                     gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAt   >  1:649156/1‑93 (MQ=255)
                                                                                     gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAGGTGGAAATTGTTCCGGGCAt   >  1:816251/1‑93 (MQ=255)
                                                                                         |                                                                                         
ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130650‑4130828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑