BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,173,376	A→G	noncoding (44/76 nt)	thrT →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,173,376	0	A	G	98.8%	547.8 / ‑3.0	174	noncoding (44/76 nt)	thrT	tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (76/95); total (76/98)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 179 total aligned reads displayed.

TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT  >  NC_000913/4173284‑4173465

   cAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGt                                                                                        <  1:2560376/93‑1 (MQ=255)

    aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                       >  2:312398/1‑93 (MQ=255)

    aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                       >  2:3058806/1‑93 (MQ=255)

     aGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCg                                                                                      >  1:484836/1‑93 (MQ=255)

      gCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCgg                                                                                     >  1:3900478/1‑93 (MQ=255)

       cTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGc                                                                                    <  1:1985024/93‑1 (MQ=255)

        tgatgaTGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCa                                                                                   >  2:246609/1‑93 (MQ=255)

         gatgatGCGGGTTCGATTCCCGCTGCCCGCTCCAAAATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAg                                                                                  >  2:3483609/1‑93 (MQ=255)

            gatgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTc                                                                               <  1:4420961/93‑1 (MQ=255)

            gatgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTc                                                                               <  1:2845707/93‑1 (MQ=255)

              tgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGa                                                                             <  2:1569417/93‑1 (MQ=255)

               gCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGaa                                                                            >  2:2347441/1‑93 (MQ=255)

                 gggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATc                                                                          <  1:4162283/93‑1 (MQ=255)

                 gggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATc                                                                          >  1:3136983/1‑93 (MQ=255)

                  ggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCt                                                                         <  1:3716280/93‑1 (MQ=255)

                    ttCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGc                                                                       <  1:2058038/93‑1 (MQ=255)

                     tCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGcc                                                                      <  2:3962326/93‑1 (MQ=255)

                     tCGATGCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGcc                                                                      <  1:4261679/93‑1 (MQ=255)

                        aTTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAt                                                                   <  2:4238653/93‑1 (MQ=255)

                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  >  1:2889462/1‑93 (MQ=255)

                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  <  1:164329/93‑1 (MQ=255)

                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  <  1:3127646/93‑1 (MQ=255)

                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  >  1:2730805/1‑93 (MQ=255)

                          tCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCa                                                                 <  2:1275192/93‑1 (MQ=255)

                          tCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCa                                                                 <  2:46003/93‑1 (MQ=255)

                           cccGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAg                                                                >  1:3725569/1‑93 (MQ=255)

                            ccGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGGTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGc                                                               >  1:3102761/1‑93 (MQ=255)

                             cGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGca                                                              <  2:730454/93‑1 (MQ=255)

                             cGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGca                                                              >  2:2117088/1‑93 (MQ=255)

                              gCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcac                                                             >  1:3515472/1‑93 (MQ=255)

                              gCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcac                                                             <  2:3999758/93‑1 (MQ=255)

                                   ccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActt                                                        <  2:3023721/93‑1 (MQ=255)

                                   ccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActt                                                        <  2:1252624/93‑1 (MQ=255)

                                    cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtc                                                       >  2:406330/1‑93 (MQ=255)

                                    cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtc                                                       <  1:3556592/93‑1 (MQ=255)

                                     gCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtct                                                      <  2:2172032/93‑1 (MQ=255)

                                      cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtctt                                                     >  1:481813/1‑93 (MQ=255)

                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    >  2:2736935/1‑93 (MQ=255)

                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    >  2:1595543/1‑93 (MQ=255)

                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    <  2:1310639/93‑1 (MQ=255)

                                         cAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtc                                                  >  2:285362/1‑93 (MQ=255)

                                          aaGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtct                                                 >  1:428555/1‑93 (MQ=255)

                                          aaGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtct                                                 <  1:1401979/93‑1 (MQ=255)

                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                >  1:4307710/1‑93 (MQ=255)

                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                >  2:1604037/1‑93 (MQ=255)

                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                <  1:682699/93‑1 (MQ=255)

                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  2:1642588/93‑1 (MQ=255)

                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  2:605240/93‑1 (MQ=255)

                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  1:3034458/93‑1 (MQ=255)

                                            gATGTGCTGATATAGCTAAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCCCTTCTTTTctcc                                               <  2:3142879/93‑1 (MQ=21)

                                             aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcct                                              >  2:4252361/1‑93 (MQ=255)

                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          >  1:808304/1‑93 (MQ=255)

                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          >  1:896010/1‑93 (MQ=255)

                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          <  1:2147668/93‑1 (MQ=255)

                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          <  1:420003/93‑1 (MQ=255)

                                                    gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                       <  2:2549988/93‑1 (MQ=255)

                                                     atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                      >  1:1972530/1‑93 (MQ=255)

                                                     atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                      <  2:3700610/93‑1 (MQ=255)

                                                      tataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                     <  1:3778974/93‑1 (MQ=255)

                                                      tataGCTCAGTTGGAAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                     >  2:1535511/1‑93 (MQ=255)

                                                        taGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttt                                   <  1:3249850/93‑1 (MQ=255)

                                                        taGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttt                                   >  2:2766333/1‑93 (MQ=255)

                                                         aGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTc                                  <  1:4362159/93‑1 (MQ=255)

                                                           cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                                >  1:453072/1‑93 (MQ=255)

                                                           cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                                >  2:4111653/1‑93 (MQ=255)

                                                            tCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtt                               <  2:534915/93‑1 (MQ=255)

                                                            tCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtt                               >  1:3354645/1‑93 (MQ=255)

                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  2:295930/93‑1 (MQ=255)

                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  2:1961170/93‑1 (MQ=255)

                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  1:3769085/93‑1 (MQ=255)

                                                              aGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCt                             >  2:1681035/1‑93 (MQ=255)

                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:3095456/1‑93 (MQ=255)

                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:2713143/1‑93 (MQ=255)

                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:3665499/1‑93 (MQ=255)

                                                               gTTGGCAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtttgt                            >  1:1006216/1‑90 (MQ=255)

                                                                ttGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGt                           <  1:3335281/93‑1 (MQ=255)

                                                                 tGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGtt                          <  2:806587/93‑1 (MQ=255)

                                                                  tgTAGAGCGCCCCCTTTGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGttt                         <  1:1322014/92‑1 (MQ=255)

                                                                    tAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAt                       <  1:2000361/93‑1 (MQ=255)

                                                                    tAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAt                       <  2:331144/93‑1 (MQ=255)

                                                                     agagCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAtt                      <  1:2568595/93‑1 (MQ=255)

                                                                       agCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGc                    <  1:1411658/93‑1 (MQ=255)

                                                                         cgcACCTTTGTTAAGGGTGGGGTCGGCAGTTCGAATCTGCGTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAt                  <  2:4420817/93‑1 (MQ=37)

                                                                          gcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAtt                 <  1:767451/93‑1 (MQ=255)

                                                                          gcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAtt                 >  1:887538/1‑93 (MQ=255)

                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                <  2:1366109/93‑1 (MQ=255)

                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                <  2:1366108/93‑1 (MQ=255)

                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                >  1:4096249/1‑93 (MQ=255)

                                                                             cccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa              >  2:464179/1‑93 (MQ=255)

                                                                              ccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaac             >  1:40862/1‑93 (MQ=255)

                                                                                ttGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaacaa           <  1:1045010/93‑1 (MQ=255)

                                                                                 tGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAg          <  1:1890757/93‑1 (MQ=255)

                                                                                    tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg       >  2:2717651/1‑93 (MQ=255)

                                                                                    tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg       <  2:1934490/93‑1 (MQ=255)

                                                                                     aaGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCgg      <  2:518993/93‑1 (MQ=255)

                                                                                     aaGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGGCgg      >  2:1739834/1‑93 (MQ=255)

                                                                                      aGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCggg     >  1:2276278/1‑93 (MQ=255)

                                                                                       aggTGGGGGCGGGAGTTCGAATCTGCCTATCCGCCCCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGc    <  2:4154915/92‑1 (MQ=255)

                                                                                        ggTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCa   <  1:3963573/93‑1 (MQ=255)

                                                                                         gTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAt  <  1:1263712/93‑1 (MQ=255)

TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT  >  NC_000913/4173284‑4173465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`