BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,295	A→G	V219A (GTA→GCA)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,295	0	A	G	98.0%	479.8 / ‑2.7	152	V219A (GTA→GCA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (91/58); total (92/60)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 157 total aligned reads displayed.

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

tGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcc                                                                                         >  2:2438497/1‑93 (MQ=255)

    gCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGccacca                                                                                     >  2:2660786/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  2:4321326/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  2:3423815/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  2:2052123/1‑93 (MQ=255)

        aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 >  2:1420436/1‑93 (MQ=255)

        aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 >  2:1508860/1‑93 (MQ=255)

        aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 >  2:1518747/1‑93 (MQ=255)

         tcgctcgcTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGc                                                                                >  2:3224748/1‑93 (MQ=255)

          cgctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGct                                                                               >  2:192389/1‑93 (MQ=255)

           cctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctc                                                                              <  2:3820797/92‑1 (MQ=255)

            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGACACCACATGctct                                                                             >  1:1151009/1‑93 (MQ=255)

              cgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTAc                                                                           >  2:4020496/1‑93 (MQ=255)

               gctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                          >  2:526694/1‑93 (MQ=255)

                ctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGc                                                                         <  2:2869177/93‑1 (MQ=255)

                 ttGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                        >  1:3249507/1‑93 (MQ=255)

                   gCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTgg                                                                      <  1:341877/93‑1 (MQ=255)

                    cTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                     >  2:1408168/1‑93 (MQ=255)

                     tCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTg                                                                    >  2:95572/1‑93 (MQ=255)

                      cAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGa                                                                   >  2:1666808/1‑93 (MQ=255)

                       acccAGGTCGTGCCCCCACAGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGAc                                                                  <  2:1029382/91‑1 (MQ=255)

                       aaCCAGGTCGTGCCACCACAGGTCCCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGAc                                                                  <  2:1504697/93‑1 (MQ=255)

                        aCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACg                                                                 >  2:836345/1‑93 (MQ=255)

                          cAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               <  1:1025576/93‑1 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  2:565336/1‑93 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  2:4291331/1‑93 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCCCCCTTCCTGCAAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTTGTGACGtt                                                               <  2:1818392/93‑1 (MQ=255)

                           aGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              <  2:2353893/93‑1 (MQ=255)

                           aGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              >  2:3607183/1‑93 (MQ=255)

                           aGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              >  2:3607184/1‑93 (MQ=255)

                            ggTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGTCACCACATGCTCTACGCTGGTGACGTTCg                                                             >  1:317325/1‑93 (MQ=255)

                             gTCGTGCCGCCGCAGGTCGCGCCCTCCCTGCTAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCgg                                                            <  2:2456878/93‑1 (MQ=255)

                               ggTGCCCCCACGGGCCGCCCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGt                                                          <  1:3554771/92‑1 (MQ=255)

                               cGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGt                                                          >  1:2861379/1‑93 (MQ=255)

                                gTGCCCCCACAGGTCGCGCCCTTCCTGCGAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         <  1:1519946/93‑1 (MQ=255)

                                gTGCCCCCACAGGTCGCCCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         <  1:1519948/93‑1 (MQ=255)

                                  gCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtttt                                                       <  2:3922942/93‑1 (MQ=255)

                                    ccccacAGGCCCCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTc                                                     <  2:3474935/91‑1 (MQ=255)

                                    caccacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTc                                                     >  1:2060637/1‑93 (MQ=255)

                                     accacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGATGTTCGGGTTTTTCa                                                    >  2:86232/1‑93 (MQ=255)

                                      ccacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   >  2:2348134/1‑93 (MQ=255)

                                      ccacAGGTCGCGCCCTCCCTGCCAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   <  2:2168284/93‑1 (MQ=255)

                                       cacaGGTCGCCCCTTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgc                                                  <  1:287643/93‑1 (MQ=255)

                                        acaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  2:754154/1‑93 (MQ=255)

                                        acaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  1:2554821/1‑93 (MQ=255)

                                        acaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  2:3510762/1‑93 (MQ=255)

                                        acaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  1:122694/1‑93 (MQ=255)

                                        acaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 >  2:4307355/1‑93 (MQ=255)

                                          aGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGcgcg                                               >  2:867787/1‑93 (MQ=255)

                                          aGGCCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGcgcg                                               <  1:1045115/93‑1 (MQ=255)

                                              cgcgCCTTCCCGGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcat                                           <  2:304150/93‑1 (MQ=255)

                                               ccgcCCCCCCTCCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatc                                          <  2:487051/92‑1 (MQ=255)

                                               ccccccTTCCTGCAAGTTAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatc                                          <  2:4394878/90‑1 (MQ=255)

                                                 gcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        >  1:1812295/1‑93 (MQ=255)

                                                 gcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCAACACATGCTCTACGCTGGTGACGTTCGGGTCTTTCAGCGCGTCATCaa                                        >  1:1812294/1‑93 (MQ=255)

                                                 ccccTCCCCGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        <  1:3996979/92‑1 (MQ=255)

                                                  cccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       >  1:1544436/1‑93 (MQ=255)

                                                  cccTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       <  2:3500877/93‑1 (MQ=255)

                                                   ccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACg                                      >  2:3778586/1‑93 (MQ=255)

                                                   ccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACg                                      <  1:472100/93‑1 (MQ=255)

                                                    cTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     >  1:179783/1‑93 (MQ=255)

                                                     ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    <  1:3785283/93‑1 (MQ=255)

                                                     ttCCTCCAAGTTAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    <  1:4073568/93‑1 (MQ=255)

                                                      tCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttt                                   >  2:2514450/1‑93 (MQ=255)

                                                      tCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttt                                   <  2:2999134/93‑1 (MQ=255)

                                                      tCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGCTCGGGTTTTTCAGCGCGTCATCAACGttt                                   >  1:4221821/1‑93 (MQ=255)

                                                       ccgcccATTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                  <  2:4740613/89‑1 (MQ=255)

                                                       ccTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                  <  1:2116299/93‑1 (MQ=255)

                                                        cTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttttt                                 >  1:170887/1‑93 (MQ=255)

                                                          tccAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttct                               <  2:4095452/92‑1 (MQ=255)

                                                          gCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttct                               >  2:898417/1‑93 (MQ=255)

                                                          gCCAGTCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTtcct                               <  1:276859/93‑1 (MQ=255)

                                                           ccAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                              >  1:4145649/1‑93 (MQ=255)

                                                           ccAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                              >  2:342175/1‑93 (MQ=255)

                                                            cactCAATTTTCCCGCCAGTACGCTTCAGTGGCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  2:1675304/90‑1 (MQ=255)

                                                            cAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  1:41777/93‑1 (MQ=255)

                                                            cAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  2:3343750/93‑1 (MQ=255)

                                                            cAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             >  2:1764043/1‑93 (MQ=255)

                                                            cAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  1:709563/93‑1 (MQ=255)

                                                              gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  2:891698/1‑93 (MQ=255)

                                                               tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGc                          >  1:1172423/1‑93 (MQ=255)

                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCTGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  1:3234054/1‑93 (MQ=255)

                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  2:2917515/1‑93 (MQ=255)

                                                                 aaTTTTCCCGCCAGCACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  1:3677843/1‑93 (MQ=255)

                                                                  aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       >  1:2087857/1‑93 (MQ=255)

                                                                   ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGaa                      >  2:4419500/1‑93 (MQ=255)

                                                                    tttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAAt                     <  1:2637215/93‑1 (MQ=255)

                                                                      tCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATAc                   >  2:2497510/1‑93 (MQ=255)

                                                                         cGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgc                <  1:2196014/93‑1 (MQ=255)

                                                                         cGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgc                <  2:4594455/93‑1 (MQ=255)

                                                                           ccAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcgc              >  1:2410434/1‑93 (MQ=255)

                                                                            cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             >  1:4429528/1‑93 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGTTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            <  1:2627912/93‑1 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            >  1:2584188/1‑93 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            <  1:4455456/93‑1 (MQ=255)

                                                                                    ttCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgc     >  2:1153400/1‑93 (MQ=255)

                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    >  1:1219330/1‑93 (MQ=255)

                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    >  1:3123111/1‑93 (MQ=255)

                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  >  1:566423/1‑93 (MQ=255)

                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  <  1:4260233/93‑1 (MQ=255)

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`