BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,280	T→C	T444A (ACC→GCC)	yjcP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,280	0	T	C	97.6%	503.0 / 0.0	165	T444A (ACC→GCC)	yjcP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (85/76); total (87/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 182 total aligned reads displayed.

ATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGTGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTGCA  >  NC_000913/4297190‑4297369

aTTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTcggcgg                                                                                         >  2:1534686/1‑93 (MQ=255)

aTTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTcggcgg                                                                                         <  1:4055432/93‑1 (MQ=255)

aTTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTcggcgg                                                                                         >  1:2942779/1‑93 (MQ=255)

aTTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTcggcgg                                                                                         <  1:1910037/93‑1 (MQ=255)

 tttCATCAACAGAATGCTATGGATCACCCGGACGCTGACCAGTATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCAGGCTTCggcggc                                                                                        >  2:2677851/1‑93 (MQ=255)

     atcaACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGt                                                                                    >  2:954638/1‑93 (MQ=255)

     atcaACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGt                                                                                    >  2:745142/1‑93 (MQ=255)

     atcaACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGt                                                                                    >  2:2210424/1‑93 (MQ=255)

      tcaACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTa                                                                                   <  1:631009/93‑1 (MQ=255)

       ccaCTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGCCATCTCTTCGGCACGCACTGGCAACCGGGCTTCGGCGGCCTGtca                                                                                  >  2:2333404/3‑91 (MQ=255)

       caACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTaa                                                                                  <  2:4249134/93‑1 (MQ=255)

       caACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTaa                                                                                  >  2:3743392/1‑93 (MQ=255)

        aaCTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAg                                                                                 >  2:116777/1‑93 (MQ=255)

         aCTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGc                                                                                >  1:1509694/1‑93 (MQ=255)

           tGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCgg                                                                              >  2:4471310/1‑93 (MQ=255)

           tGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCgg                                                                              <  1:481661/93‑1 (MQ=255)

           tGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCgg                                                                              <  2:2014360/93‑1 (MQ=255)

           tGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCgg                                                                              >  1:261697/1‑93 (MQ=255)

            gAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGc                                                                             <  2:2513591/93‑1 (MQ=255)

             aaTGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCt                                                                            >  2:1914316/1‑93 (MQ=255)

                gCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGt                                                                         >  1:127077/1‑93 (MQ=255)

                 cTTTGGATCACCCGGCGGCTGTTCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGtt                                                                        <  2:39899/93‑1 (MQ=255)

                  tttGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGCCCTGAAAGCGGCTGGTTa                                                                       >  2:3726323/1‑93 (MQ=255)

                   ttGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTaa                                                                      >  2:3251302/1‑93 (MQ=255)

                     ggATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGc                                                                    >  2:4254280/1‑93 (MQ=255)

                        tCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCcgc                                                                 >  2:184262/1‑93 (MQ=255)

                         cACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCcgcg                                                                >  1:4325222/1‑93 (MQ=255)

                          ccccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCgcgc                                                               <  2:2048190/92‑1 (MQ=255)

                          aCCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCgcgc                                                               >  2:3669864/1‑93 (MQ=255)

                          aCCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGAGGCTGGTTAAGCCgcgc                                                               >  1:2471482/1‑93 (MQ=255)

                           cccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCt                                                              >  2:1963630/1‑93 (MQ=255)

                           cccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCt                                                              >  1:3682363/1‑93 (MQ=255)

                            ccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTg                                                             <  2:2732257/93‑1 (MQ=255)

                            ccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTg                                                             >  1:4439623/1‑93 (MQ=255)

                            ccGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTg                                                             >  2:3621097/1‑93 (MQ=255)

                             cggcggCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTgt                                                            >  1:1776725/1‑92 (MQ=255)

                             cggcggCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGa                                                            >  2:1337515/1‑93 (MQ=255)

                                cggcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAg                                                         <  1:3719629/93‑1 (MQ=255)

                                  gcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggc                                                       <  1:4476176/93‑1 (MQ=255)

                                  gcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggc                                                       <  1:3316739/93‑1 (MQ=255)

                                  gcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggc                                                       <  2:3325413/93‑1 (MQ=255)

                                  gcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggc                                                       >  1:420242/1‑93 (MQ=255)

                                  gcTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggc                                                       >  1:762251/1‑93 (MQ=255)

                                    tGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggcgg                                                     <  2:2802135/93‑1 (MQ=255)

                                     gTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAggcggc                                                    >  2:1879070/1‑93 (MQ=255)

                                       ccAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCt                                                  <  1:2069111/93‑1 (MQ=255)

                                          gCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCgg                                               >  2:556116/1‑93 (MQ=255)

                                          gCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCgg                                               >  1:2152796/1‑93 (MQ=255)

                                            aTCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCa                                             <  2:618063/93‑1 (MQ=255)

                                             tCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAg                                            >  1:169055/1‑93 (MQ=255)

                                             tCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAg                                            >  2:4306352/1‑93 (MQ=255)

                                             tCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAg                                            >  2:3510308/1‑93 (MQ=255)

                                               aGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAgcg                                          >  1:1108859/1‑93 (MQ=255)

                                                gTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAgcgc                                         <  1:548148/93‑1 (MQ=255)

                                                   aTGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCt                                      <  1:668395/93‑1 (MQ=255)

                                                   aTGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCt                                      <  2:948236/93‑1 (MQ=255)

                                                     gACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTgg                                    <  1:4379603/93‑1 (MQ=255)

                                                        aTCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTa                                 >  2:2947796/1‑93 (MQ=255)

                                                         tctcTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTaa                                >  1:839225/1‑93 (MQ=255)

                                                         tctcTTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTaa                                >  1:1782280/1‑93 (MQ=255)

                                                         cctctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTaa                                <  2:3640022/92‑1 (MQ=255)

                                                          ctctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTaaa                               <  2:2371934/93‑1 (MQ=255)

                                                          ctctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTaaa                               <  1:2947041/93‑1 (MQ=255)

                                                           tctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGGCTCGGCAGGGCGCTGGGTaaaa                              >  2:784762/1‑92 (MQ=255)

                                                           tctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAg                              >  2:2088454/1‑93 (MQ=255)

                                                           tctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAg                              >  1:4443451/1‑93 (MQ=255)

                                                           tctTCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAg                              >  1:1466085/1‑93 (MQ=255)

                                                             ttCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAACGCGCTGGGTAAAgcg                            >  1:342970/1‑93 (MQ=255)

                                                              tCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAgcgc                           >  1:1069504/1‑93 (MQ=255)

                                                              tCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAgcgc                           >  2:3320682/1‑93 (MQ=255)

                                                              tCGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAgcgc                           <  1:500289/93‑1 (MQ=255)

                                                               cGGCAAGCACTGGCAACCGGGCTTCGGTGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGcgcg                          <  2:1585390/93‑1 (MQ=255)

                                                               cGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGcgcg                          >  2:4320032/1‑93 (MQ=255)

                                                               cGGCAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGcgcg                          <  2:4410284/93‑1 (MQ=255)

                                                                 gCAAGCACAGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTg                        <  1:876585/93‑1 (MQ=255)

                                                                  cAAGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTgg                       >  1:927590/1‑93 (MQ=255)

                                                                   aaGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGc                      >  2:3809260/1‑93 (MQ=255)

                                                                    aGCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCt                     >  2:878005/1‑93 (MQ=255)

                                                                     gCACTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCtt                    >  2:544625/1‑93 (MQ=255)

                                                                       aCTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTcc                  <  1:3187281/93‑1 (MQ=255)

                                                                        cTGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCa                 <  2:687008/93‑1 (MQ=255)

                                                                        cTGGCAACAGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCa                 <  1:140615/93‑1 (MQ=255)

                                                                         tGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCAc                >  1:1335672/1‑93 (MQ=255)

                                                                         tGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCAc                <  1:705867/93‑1 (MQ=255)

                                                                         tGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCAc                <  1:3544958/93‑1 (MQ=255)

                                                                         tGGCAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCAc                <  1:4493516/93‑1 (MQ=255)

                                                                            cAACCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCAcgcg             >  1:987925/1‑93 (MQ=255)

                                                                             aaCCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGt            >  1:3279635/1‑93 (MQ=255)

                                                                              aCCGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGtt           <  1:3532841/93‑1 (MQ=255)

                                                                              aCCGGGCTTCGGCGGCCTGTAAGAGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGGGGCTTCCCCGCCtt           >  2:3932929/1‑93 (MQ=255)

                                                                                ctggCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCa         <  2:241880/91‑1 (MQ=255)

                                                                                cGGGCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCa         >  1:3271352/1‑93 (MQ=255)

                                                                                 gggCTTCGGTGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAg        <  1:1579880/93‑1 (MQ=255)

                                                                                   gCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAggc      >  2:2812375/1‑91 (MQ=255)

                                                                                   gCTTCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGcc      <  2:3470092/93‑1 (MQ=255)

                                                                                    cTTCGGCGGCCTGTAAGCGGCTGGCTAAGCCGCGCTGGTAGGCGGCCTCTGCAGCGCGCTGGGCGAAGCGCGTGGCTTCCACGCGCTCAGACt     >  2:52224/1‑93 (MQ=255)

                                                                                     ttCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTg    >  2:3956204/1‑93 (MQ=255)

                                                                                     ttCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTg    >  1:2508613/1‑93 (MQ=255)

                                                                                      tCGGCGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTGc   >  2:4652885/1‑93 (MQ=255)

                                                                                       cggcggCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTGCa  <  1:2346282/93‑1 (MQ=255)

ATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGCTTCGGTGGCCTGTAAGCGGCTGGTTAAGCCGCGCTGATAGGCGGCCTCGGCAGCGCGCTGGGTAAAGCGCGTGGCTTCCACGCGTTCAGCCTGCA  >  NC_000913/4297190‑4297369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`