BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,381,510	T→C	Y46H (TAC→CAC)	yjeM →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,381,510	0	T	C	97.7%	824.5 / 5.1	261	Y46H (TAC→CAC)	yjeM	putative transport
Reads supporting (aligned to +/- strand): ref base T (3/3); new base C (100/155); total (103/158)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

Only 100 of 268 total aligned reads displayed.

CTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGA  >  NC_000913/4381421‑4381599

cTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACt                                                                                        <  1:656304/93‑1 (MQ=255)

 tCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGTATTTGCCAATAGCCCATCGGCTTATCACtt                                                                                       <  1:3753897/93‑1 (MQ=255)

  cacaCGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTa                                                                                      >  2:2499051/1‑93 (MQ=255)

  cacaCGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTa                                                                                      <  2:2881634/93‑1 (MQ=255)

   acacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTaa                                                                                     >  1:3647477/1‑93 (MQ=255)

    cacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAAt                                                                                    <  1:4068139/93‑1 (MQ=255)

    cacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAAt                                                                                    <  1:1689872/93‑1 (MQ=255)

    cacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAAt                                                                                    <  1:3163682/93‑1 (MQ=255)

     acGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCTATCGGCTTATCACTTAATg                                                                                   >  1:3252975/1‑93 (MQ=255)

     acGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATg                                                                                   >  2:2342302/1‑93 (MQ=255)

     acGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATg                                                                                   <  2:2619781/93‑1 (MQ=255)

       gATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATggg                                                                                 >  2:2343050/1‑93 (MQ=255)

        aTAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGt                                                                                <  2:4439657/93‑1 (MQ=255)

        aTAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGt                                                                                >  1:1639274/1‑93 (MQ=255)

         tAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGtt                                                                               <  2:1726761/93‑1 (MQ=255)

         tAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGtt                                                                               <  1:2299795/93‑1 (MQ=255)

            aaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTtata                                                                            <  2:1973310/93‑1 (MQ=255)

             aaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAg                                                                           <  2:3586557/93‑1 (MQ=255)

             aaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAg                                                                           >  2:4606374/1‑93 (MQ=255)

             aaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAg                                                                           <  1:1464720/93‑1 (MQ=255)

              aaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGt                                                                          <  2:590744/93‑1 (MQ=255)

               aGATGAGTCTGATAGGACTCATGTTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTg                                                                         >  2:2245250/1‑93 (MQ=255)

               aGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTg                                                                         <  2:3886340/93‑1 (MQ=255)

               aGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTg                                                                         <  2:1823120/93‑1 (MQ=255)

               aGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTg                                                                         <  1:2995916/93‑1 (MQ=255)

               aGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTg                                                                         >  1:3488245/1‑93 (MQ=255)

                gATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGc                                                                        <  2:3357172/93‑1 (MQ=255)

                 aTGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCg                                                                       >  2:4372972/1‑93 (MQ=255)

                 aTGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCg                                                                       >  1:2267634/1‑93 (MQ=255)

                 aTGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCg                                                                       <  2:4020066/93‑1 (MQ=255)

                  tGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGa                                                                      >  1:558722/1‑93 (MQ=255)

                  tGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAAGGGGTTATAGTGCGa                                                                      <  1:3244797/93‑1 (MQ=255)

                   gAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGAt                                                                     >  1:1086786/1‑93 (MQ=255)

                   gAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGAt                                                                     >  2:2815211/1‑93 (MQ=255)

                      tCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTcc                                                                  <  2:1097503/93‑1 (MQ=255)

                       cTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTccc                                                                 <  1:1029024/93‑1 (MQ=255)

                         gATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCtt                                                               <  2:3258378/93‑1 (MQ=255)

                          aTAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCttt                                                              >  2:4168956/1‑93 (MQ=255)

                          aTAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCttt                                                              <  1:426020/93‑1 (MQ=255)

                          aTAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCttt                                                              >  1:45400/1‑93 (MQ=255)

                            aGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTta                                                            <  1:3428299/93‑1 (MQ=255)

                            aGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTta                                                            >  2:374731/1‑93 (MQ=255)

                            aGGACTCATATTGATGAACTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTta                                                            <  1:4242806/93‑1 (MQ=255)

                             ggACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTtat                                                           <  1:1822405/93‑1 (MQ=255)

                             ggACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTtat                                                           <  1:4709947/93‑1 (MQ=255)

                              gACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTtata                                                          >  1:3894374/1‑93 (MQ=255)

                               aCTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTtatat                                                         <  1:1522712/93‑1 (MQ=255)

                                cTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTtatata                                                        <  1:1796275/93‑1 (MQ=255)

                                 tCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTatatat                                                       <  2:2369033/93‑1 (MQ=255)

                                  cATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATAtt                                                      >  1:2378323/1‑93 (MQ=255)

                                     atTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATAttttt                                                   >  1:1165815/1‑92 (MQ=255)

                                      ttGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCt                                                  >  1:2471643/1‑93 (MQ=255)

                                         atgatCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCa                                               <  2:1475155/93‑1 (MQ=255)

                                          tgatCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCAt                                              <  1:1266002/93‑1 (MQ=255)

                                            atCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTg                                            >  2:186351/1‑93 (MQ=255)

                                             tCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGt                                           >  1:4160250/1‑93 (MQ=255)

                                             tCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGt                                           >  2:1244227/1‑93 (MQ=255)

                                               tttACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTa                                         <  2:2937141/93‑1 (MQ=255)

                                                ttACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTAt                                        <  1:1338176/93‑1 (MQ=255)

                                                 tACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTAtt                                       >  2:3818005/1‑93 (MQ=255)

                                                  tcTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTc                                      <  2:878140/92‑1 (MQ=255)

                                                  aCTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTc                                      >  1:3287627/1‑93 (MQ=255)

                                                   cTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCt                                     <  2:2548196/93‑1 (MQ=255)

                                                   cTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCt                                     <  1:2735624/93‑1 (MQ=255)

                                                     tCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCttt                                   <  2:2192843/93‑1 (MQ=255)

                                                     tCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCttt                                   >  2:2997142/1‑93 (MQ=255)

                                                      tcGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTa                                  <  1:1595599/92‑1 (MQ=255)

                                                      ccGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTa                                  <  1:2631083/93‑1 (MQ=255)

                                                       cGTATTTGGATTTGCCACTAGCCCATCGGCTTATCAATTAATGAGTTATAGTGCGATTCCCTTTTATAAATTTTCTGCATTGTTATTCTTAAt                                 <  1:3613551/93‑1 (MQ=255)

                                                       cGTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTAt                                 <  1:990960/93‑1 (MQ=255)

                                                       cGTATTAGGATTTGCCATTCGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTAAATATTTTCAGCATTGTTATTCTTTAt                                 <  2:4224082/93‑1 (MQ=255)

                                                        gTATTTGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTAtt                                <  1:135042/93‑1 (MQ=255)

                                                         tATTTGGATTTGCCAATAGCCCATCGGCTTATCGCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTc                               >  2:4012657/1‑93 (MQ=255)

                                                            ttGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCAt                            <  2:3783687/93‑1 (MQ=255)

                                                             tGGATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCAtt                           <  1:444368/93‑1 (MQ=255)

                                                              ggATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTc                          <  2:4395622/93‑1 (MQ=255)

                                                              ggATTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTc                          <  2:3405113/93‑1 (MQ=255)

                                                                aTTTGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGc                        >  2:212796/1‑93 (MQ=255)

                                                                  ttGCCAATAGCCCATCGGCTTATCATTTAATGGGTTATAGTGCGATTCCCTTTTATATTTTTTCTGCATTGTTATTCTTTATTCCATTCGCCt                      <  2:3694633/93‑1 (MQ=255)

                                                                   tGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCtt                     <  2:1485234/93‑1 (MQ=255)

                                                                   tGCCAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCtt                     <  2:1586192/93‑1 (MQ=255)

                                                                     ccAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTaa                   <  2:1341466/93‑1 (MQ=255)

                                                                      cAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAat                  >  1:2875792/1‑93 (MQ=255)

                                                                      cAATAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAat                  >  1:1537965/1‑93 (MQ=255)

                                                                        aTAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatga                <  2:165930/93‑1 (MQ=255)

                                                                         tAGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatgat               <  2:4320606/93‑1 (MQ=255)

                                                                          aGCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatgatg              <  2:2016498/93‑1 (MQ=255)

                                                                           gCCCATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATgg             >  1:3388710/1‑93 (MQ=255)

                                                                              cATCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTg          >  1:2176434/1‑93 (MQ=255)

                                                                                tCGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGaa        <  2:2293385/93‑1 (MQ=255)

                                                                                 cGGCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGaaa       <  2:1685095/93‑1 (MQ=255)

                                                                                  ggCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAAt      >  2:4271478/1‑93 (MQ=255)

                                                                                  ggCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAAt      <  1:784063/93‑1 (MQ=255)

                                                                                   gCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATg     >  1:700201/1‑93 (MQ=255)

                                                                                   gCTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTACTCCATTCGCCTTAATGATGGCTGAAATg     <  2:4729882/93‑1 (MQ=255)

                                                                                    cTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATgg    <  1:2037340/93‑1 (MQ=255)

                                                                                    cTTATCACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATgg    <  2:2467966/93‑1 (MQ=255)

                                                                                     ttatcaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATggg   >  2:4039011/1‑93 (MQ=255)

                                                                                     ttatcaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATggg   <  2:3167745/93‑1 (MQ=255)

                                                                                      tatcaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGa  >  2:2065241/1‑93 (MQ=255)

CTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGA  >  NC_000913/4381421‑4381599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`