Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 613,409 A→G A10A (GCA→GCG)  entF → ATP‑dependent serine activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913613,4090AG97.6% 506.9 / 0.6 167A10A (GCA→GCG) entFATP‑dependent serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (88/75);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 176 total aligned reads displayed.

GGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGA  >  NC_000913/613319‑613498
                                                                                          |                                                                                         
ggCTGGAGGCCCCCTGGCGTACTGTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGcgca                                                                                         <  2:1416220/93‑1 (MQ=255)
ggCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGcgca                                                                                         >  1:2488218/1‑93 (MQ=255)
ggCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGcgca                                                                                         >  1:1948084/1‑93 (MQ=255)
    ggAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGccc                                                                                     <  2:2926398/93‑1 (MQ=255)
    ggAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGccc                                                                                     >  2:1110186/1‑93 (MQ=255)
    ggAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGccc                                                                                     >  2:1292621/1‑93 (MQ=255)
    ggAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGccc                                                                                     >  2:4705645/1‑93 (MQ=255)
    ggAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGccc                                                                                     >  2:4431414/1‑93 (MQ=255)
      aaGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCgg                                                                                   >  2:4290624/1‑93 (MQ=255)
       aGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGc                                                                                  >  1:4140395/1‑93 (MQ=255)
       aGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGc                                                                                  <  1:572938/93‑1 (MQ=255)
        gCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCa                                                                                 <  2:433608/93‑1 (MQ=255)
        gCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCa                                                                                 >  1:209243/1‑93 (MQ=255)
         cccACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCAt                                                                                >  1:3783615/1‑93 (MQ=255)
         cccACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCAt                                                                                <  2:4076659/93‑1 (MQ=255)
         cccACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCAt                                                                                >  2:2766560/1‑93 (MQ=255)
           cACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCt                                                                              >  1:1657360/1‑93 (MQ=255)
           cACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCt                                                                              <  1:3428510/93‑1 (MQ=255)
           cACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCt                                                                              >  2:1802001/1‑93 (MQ=255)
               ggCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGAt                                                                          >  1:263399/1‑93 (MQ=255)
                 cGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATgg                                                                        >  1:3359079/1‑93 (MQ=255)
                 cGTACTCTGACACCGACAAATTTTACCCAGTTGCAGGAGGCACAATGAGCAAGCATTTACTTTTGGTCGCCGCGCAGCCCGGCATCTGGAggg                                                                        <  2:1476471/93‑1 (MQ=255)
                    aCTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAg                                                                     <  1:637746/93‑1 (MQ=255)
                    aCTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAg                                                                     >  2:2395220/1‑93 (MQ=255)
                      tctGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGaa                                                                   >  2:1090101/1‑93 (MQ=255)
                      tctGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGaa                                                                   >  1:4191681/1‑93 (MQ=255)
                      tctGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGaa                                                                   >  1:3750461/1‑93 (MQ=255)
                        tGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTCCCTTTGGTCGCCGCGCCGCCCGGCATCTGGATGGCAGaaaa                                                                 <  2:512584/93‑1 (MQ=255)
                        tGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCCCACAGCCCGGCATCTGGATGGCAGaaaa                                                                 <  2:3826736/93‑1 (MQ=255)
                         gACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGaaaaa                                                                >  2:1422713/1‑93 (MQ=255)
                          acacCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAAc                                                               <  2:1122736/93‑1 (MQ=255)
                          acacCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAAc                                                               >  2:4520580/1‑93 (MQ=255)
                          acacCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAAc                                                               <  2:3069680/93‑1 (MQ=255)
                           cacCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACt                                                              <  1:1378288/93‑1 (MQ=255)
                            acCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTg                                                             >  1:1189056/1‑93 (MQ=255)
                            acCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTg                                                             >  1:1536661/1‑93 (MQ=255)
                            acCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTg                                                             >  1:2232728/1‑93 (MQ=255)
                             ccGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCTCGCAGCCCGGCATCTGGATGGCAGAAAAACTGt                                                            <  1:1802161/93‑1 (MQ=255)
                             ccGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGTCATCTGGATGGCAGAAAAACTGt                                                            >  2:2583480/1‑93 (MQ=255)
                              cgacgaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTc                                                           >  2:1846634/1‑93 (MQ=255)
                               gacgaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCa                                                          <  2:3585866/93‑1 (MQ=255)
                               gacgaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCa                                                          <  1:482979/93‑1 (MQ=255)
                                acgaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAg                                                         >  2:2172443/1‑93 (MQ=255)
                                acgaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAg                                                         >  1:2997018/1‑93 (MQ=255)
                                  gaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGaa                                                       <  2:2314881/93‑1 (MQ=255)
                                  gaATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGaa                                                       <  2:3219282/93‑1 (MQ=255)
                                   aaTTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAAt                                                      >  1:1235462/1‑93 (MQ=255)
                                    aTTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAAtt                                                     >  1:3282843/1‑93 (MQ=255)
                                        tACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTAccc                                                 <  2:212650/93‑1 (MQ=255)
                                        tACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTAccc                                                 <  1:2110512/93‑1 (MQ=255)
                                          cccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTc                                               >  2:3659813/1‑93 (MQ=255)
                                          cccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTc                                               >  2:1202650/1‑93 (MQ=255)
                                          cccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTc                                               <  2:2854094/93‑1 (MQ=255)
                                           ccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTcc                                              >  2:3537879/1‑93 (MQ=255)
                                           ccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTcc                                              <  2:3736082/93‑1 (MQ=255)
                                           ccAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTcc                                              <  1:1218725/93‑1 (MQ=255)
                                            cAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCg                                             <  2:1145345/93‑1 (MQ=255)
                                             aGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGc                                            >  2:2009246/1‑93 (MQ=255)
                                             aGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGc                                            >  1:4672014/1‑93 (MQ=255)
                                              gTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGcc                                           <  1:67416/93‑1 (MQ=255)
                                              gTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGcc                                           <  2:302913/93‑1 (MQ=255)
                                                tGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTg                                         <  2:1747302/93‑1 (MQ=255)
                                                 gCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTgg                                        >  1:4695551/1‑93 (MQ=255)
                                                 gCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTgg                                        >  1:817511/1‑93 (MQ=255)
                                                  cAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTGCCCTCCGCCTGGa                                       <  1:3135056/93‑1 (MQ=255)
                                                  cAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGa                                       <  1:4518763/93‑1 (MQ=255)
                                                  cAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGa                                       >  2:3383789/1‑93 (MQ=255)
                                                   aggaggCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAg                                      >  1:4365757/1‑93 (MQ=255)
                                                   aggaggCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAg                                      >  2:3950644/1‑93 (MQ=255)
                                                       ggCACAATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTg                                  >  1:953838/1‑93 (MQ=255)
                                                         cacaATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGgc                                <  2:870281/93‑1 (MQ=255)
                                                         cacaATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGgc                                <  1:2951617/93‑1 (MQ=255)
                                                           caATGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGgcgc                              >  1:1435308/1‑93 (MQ=255)
                                                             aTGAGCCAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCAt                            >  2:3391101/1‑93 (MQ=255)
                                                                  ccAGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGt                       >  2:4141259/1‑93 (MQ=255)
                                                                    aGCATTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTg                     >  1:2631694/1‑93 (MQ=255)
                                                                       aTTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGt                  <  2:2395858/93‑1 (MQ=255)
                                                                       aTTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGt                  <  2:604407/93‑1 (MQ=255)
                                                                       aTTTACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGt                  <  1:1880113/93‑1 (MQ=255)
                                                                        tttACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGtt                 >  1:355010/1‑93 (MQ=255)
                                                                        tttACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGtt                 >  1:4417786/1‑93 (MQ=255)
                                                                          tACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTaa               <  1:3676358/93‑1 (MQ=255)
                                                                          tACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTaa               <  1:3083506/93‑1 (MQ=255)
                                                                          tACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTaa               <  2:1605540/93‑1 (MQ=255)
                                                                          tACCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTaa               <  1:855039/93‑1 (MQ=255)
                                                                           tccTTTGGTCGCCTCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAAc              <  2:3416076/92‑1 (MQ=255)
                                                                           aCCTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTTCGTTGAGTTAAc              >  1:1267510/1‑93 (MQ=255)
                                                                            ccTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTCCCCTCCGCCTGGAGCGTGGCGCA‑TACGTTGAGTTAACCg            <  1:1282547/93‑1 (MQ=255)
                                                                             cTTTGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCg            <  1:3013653/93‑1 (MQ=255)
                                                                              tttGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCgg           >  2:2302961/1‑93 (MQ=255)
                                                                              tttGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCgg           >  2:772474/1‑93 (MQ=255)
                                                                              tttGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCgg           >  1:972163/1‑93 (MQ=255)
                                                                              tttGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCgg           >  1:1645640/1‑93 (MQ=255)
                                                                               ttGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGga          >  2:4732758/1‑93 (MQ=255)
                                                                                tGGTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGgag         >  1:2469008/1‑93 (MQ=255)
                                                                                 ggTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGgaga        <  1:6995/93‑1 (MQ=255)
                                                                                  gTCGCCGCGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGagag       <  1:4341239/93‑1 (MQ=255)
                                                                                    cgccgcGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGt     <  1:1064443/93‑1 (MQ=255)
                                                                                     gccgcGCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGtt    <  1:1348604/93‑1 (MQ=255)
                                                                                       cgcgCAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGa  >  1:3973373/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAGCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGA  >  NC_000913/613319‑613498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑