BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,428,092	A→G	G217G (GGA→GGG)	cycA →	transport of D‑alanine, D‑serine, and glycine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,428,092	0	A	G	100.0%	444.1 / NA	136	G217G (GGA→GGG)	cycA	transport of D‑alanine, D‑serine, and glycine
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (65/71); total (65/71)

Only 100 of 140 total aligned reads displayed.

TCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAC  >  NC_000913/4428002‑4428180

tCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGtt                                                                                        >  2:1229777/1‑93 (MQ=255)

tCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGtt                                                                                        <  2:1786373/93‑1 (MQ=255)

 cAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTc                                                                                       >  2:796783/1‑93 (MQ=255)

  aGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTcc                                                                                      <  1:3800926/93‑1 (MQ=255)

   gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCa                                                                                     >  2:1117563/1‑93 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                  <  2:3439490/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                  <  2:381775/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                  >  2:2796948/1‑93 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                  <  1:135868/93‑1 (MQ=255)

        cGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAg                                                                                <  1:332017/93‑1 (MQ=255)

         gACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGc                                                                               <  2:4589347/93‑1 (MQ=255)

         gACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGc                                                                               >  1:2296151/1‑93 (MQ=255)

          aCTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCg                                                                              <  1:2319575/93‑1 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCCGATAGCgg                                                                             >  2:1832861/1‑93 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                             <  2:3979277/93‑1 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                             <  2:3979276/93‑1 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                             <  2:680582/93‑1 (MQ=255)

              gtgtGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGttt                                                                          >  1:4269177/1‑93 (MQ=255)

               tgtgGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtttt                                                                         >  2:3327400/1‑93 (MQ=255)

                  ggAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGc                                                                      <  1:3422368/93‑1 (MQ=255)

                   gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCt                                                                     <  2:487450/93‑1 (MQ=255)

                     aGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCttt                                                                   >  1:557587/1‑93 (MQ=255)

                     aGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATACCGGTTTTCGCttt                                                                   >  2:3281603/1‑93 (MQ=255)

                       cGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCg                                                                 <  1:4590625/93‑1 (MQ=255)

                       cGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCg                                                                 <  1:4590626/93‑1 (MQ=255)

                       cGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCg                                                                 <  1:753010/93‑1 (MQ=255)

                        gTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGATCCAGATAGCGGTTTTCGCTTTCGt                                                                >  1:600150/1‑93 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                               <  2:1140123/93‑1 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGGTTTCGCTTTCGTg                                                               >  1:4203922/1‑93 (MQ=255)

                          cATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgg                                                              >  1:2235453/1‑93 (MQ=255)

                          cATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgg                                                              >  2:3159964/1‑93 (MQ=255)

                           aTTCGCGCATTTGTGGAATGACGGCGGCTGTTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTggg                                                             <  1:2171687/93‑1 (MQ=255)

                           aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTggg                                                             >  2:183706/1‑93 (MQ=255)

                            ttCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgggg                                                            >  1:4314188/1‑93 (MQ=255)

                                cgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg                                                        >  2:2715406/1‑93 (MQ=255)

                                 gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                       <  1:975086/93‑1 (MQ=255)

                                 gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                       >  1:4619026/1‑93 (MQ=255)

                                 gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                       <  1:4033857/93‑1 (MQ=255)

                                 gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                       >  2:3058740/1‑93 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGc                                                     >  2:2433296/1‑93 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGc                                                     >  1:4031594/1‑93 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGc                                                     <  2:602098/93‑1 (MQ=255)

                                    tttGTGGAATGACGGCGACTGATTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGTATTGAGCt                                                    <  2:4295384/93‑1 (MQ=255)

                                     ttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTg                                                   >  1:1350184/1‑93 (MQ=255)

                                      tgtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTgg                                                  <  1:1785468/93‑1 (MQ=255)

                                       gtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggt                                                 >  1:1662934/1‑93 (MQ=255)

                                       gtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggt                                                 <  2:2268198/93‑1 (MQ=255)

                                        tgGAATTACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggta                                                <  1:393538/93‑1 (MQ=255)

                                         ggAATGACGGCGGCTGGTTCACGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtag                                               <  1:3046661/93‑1 (MQ=255)

                                          gAATGACGTCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtagg                                              <  1:156818/93‑1 (MQ=255)

                                           aaTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtaggt                                             <  2:4274360/93‑1 (MQ=255)

                                           aaTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtaggt                                             <  1:4718194/93‑1 (MQ=255)

                                            aTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtaggta                                            >  2:2432000/1‑93 (MQ=255)

                                            aTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtaggta                                            <  1:1875084/93‑1 (MQ=255)

                                              gACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGGTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTaca                                          >  2:4281059/1‑93 (MQ=255)

                                               aCGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaa                                         >  1:2051776/1‑93 (MQ=255)

                                               aCGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCATGGGGATTGAGCTGGTAGGTacaa                                         <  1:782540/93‑1 (MQ=255)

                                                cggcggCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaac                                        <  1:2871670/93‑1 (MQ=255)

                                                 ggcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaaca                                       <  1:1826748/93‑1 (MQ=255)

                                                 ggcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaaca                                       >  1:3572692/1‑93 (MQ=255)

                                                  gcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAg                                      <  1:4159540/93‑1 (MQ=255)

                                                  gcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAg                                      <  1:4159542/93‑1 (MQ=255)

                                                   cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                     <  1:1765530/93‑1 (MQ=255)

                                                    ggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCt                                    >  2:355089/1‑93 (MQ=255)

                                                     gcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTg                                   >  2:4282682/1‑93 (MQ=255)

                                                     gcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTg                                   >  2:4536552/1‑93 (MQ=255)

                                                       tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCg                                 >  2:2324251/1‑93 (MQ=255)

                                                       tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCg                                 <  2:3751915/93‑1 (MQ=255)

                                                           tCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGaaa                             >  2:1854126/1‑93 (MQ=255)

                                                            cccGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAc                            <  1:1920392/93‑1 (MQ=255)

                                                             ccGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAcc                           <  1:2774699/93‑1 (MQ=255)

                                                             ccGAAAGGATTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAcc                           >  1:3020749/1‑93 (MQ=255)

                                                              cGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCa                          >  1:1107666/1‑93 (MQ=255)

                                                               gAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaa                         <  2:2511550/93‑1 (MQ=255)

                                                               gAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaa                         >  1:2580746/1‑93 (MQ=255)

                                                               gAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaa                         >  1:3929869/1‑93 (MQ=255)

                                                                 aaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAg                       >  1:2068761/1‑93 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                      <  2:899858/93‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                      >  1:3327763/1‑93 (MQ=255)

                                                                    gTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATc                    >  1:538520/1‑93 (MQ=255)

                                                                    gTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATc                    >  1:3742274/1‑93 (MQ=255)

                                                                    gTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATc                    >  2:4179141/1‑93 (MQ=255)

                                                                      ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                  <  2:372344/93‑1 (MQ=255)

                                                                        aaGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCaga                <  1:2331156/93‑1 (MQ=255)

                                                                        aaGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCaga                >  2:562230/1‑93 (MQ=255)

                                                                         aGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCagag               <  2:3683941/93‑1 (MQ=255)

                                                                          gTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAgaga              >  1:1741220/1‑93 (MQ=255)

                                                                           tGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGaa             <  1:4513270/93‑1 (MQ=255)

                                                                           tGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGaa             <  2:2734880/93‑1 (MQ=255)

                                                                            ggCTTCTTTGCCGGGTACCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGaaa            >  2:4507995/1‑93 (MQ=255)

                                                                             gCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAt           >  1:4112410/1‑93 (MQ=255)

                                                                             gCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAt           >  2:2817675/1‑93 (MQ=255)

                                                                             gCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAt           >  2:2817677/1‑93 (MQ=255)

                                                                              cttcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATc          <  1:3313063/93‑1 (MQ=255)

                                                                              cttcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATc          >  1:3153539/1‑93 (MQ=255)

                                                                               ttcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCa         >  1:317560/1‑93 (MQ=255)

                                                                                tctgTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc        <  1:3795593/93‑1 (MQ=255)

                                                                                 cttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACt       <  2:4180486/93‑1 (MQ=255)

                                                                                 cttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACt       <  1:3191605/93‑1 (MQ=255)

                                                                                 cttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACt       <  1:2590025/93‑1 (MQ=255)

TCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAC  >  NC_000913/4428002‑4428180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`