BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,689	C→T	D135D (GAC→GAT)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,689	0	C	T	100.0%	1054.7 / NA	294	D135D (GAC→GAT)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (151/143); total (151/143)

Only 100 of 300 total aligned reads displayed.

GATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AA‑ACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435598‑4435778

   ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAgatgat                                                                                        <  2:1013216/93‑1 (MQ=255)

   ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAgatgat                                                                                        <  2:2732883/93‑1 (MQ=255)

       aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTa                                                                                    >  2:1477203/1‑93 (MQ=255)

       aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTa                                                                                    <  1:3911904/93‑1 (MQ=255)

        tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTAt                                                                                   >  1:1065157/1‑93 (MQ=255)

         aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATg                                                                                  >  1:1024379/1‑93 (MQ=255)

          cGAAGAAATACCCATAACAATAAATGAATATAAGTTATTCATGGATTTCAAAAACCGTGAATGTCCGTTAGAACAAA‑ACAGATGATTGTATGc                                                                                 >  2:2530866/1‑93 (MQ=255)

          cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGc                                                                                 <  2:4646779/93‑1 (MQ=255)

           gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAGCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGcc                                                                                <  1:2925631/93‑1 (MQ=255)

           gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGcc                                                                                <  1:2638100/93‑1 (MQ=255)

            aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCt                                                                               <  1:3802619/93‑1 (MQ=255)

             agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTg                                                                              <  1:758943/93‑1 (MQ=255)

             agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTg                                                                              >  2:800162/1‑93 (MQ=255)

             agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTg                                                                              <  1:3122009/93‑1 (MQ=255)

             agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTg                                                                              >  2:4335271/1‑93 (MQ=255)

             agaaATACCCATAAAAATAAATGAATATAACTCATTCATGGATTAAAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTg                                                                              <  2:2432990/93‑1 (MQ=255)

              gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTgg                                                                             <  2:1567633/93‑1 (MQ=255)

                aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGa                                                                           >  1:1941415/1‑93 (MQ=255)

                 aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGat                                                                          <  1:2963288/93‑1 (MQ=255)

                   aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGatat                                                                        >  2:1420933/1‑93 (MQ=255)

                     ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTc                                                                      >  2:3272119/1‑93 (MQ=255)

                     ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTc                                                                      >  1:3059544/1‑93 (MQ=255)

                      cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCt                                                                     >  2:3631706/1‑93 (MQ=255)

                      cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCt                                                                     >  1:1630308/1‑93 (MQ=255)

                      cATAACAATAAATGAATATAACTCATGCATGGATTTCAAAACCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCt                                                                     <  1:4036647/93‑1 (MQ=255)

                       aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTa                                                                    >  2:2081227/1‑93 (MQ=255)

                          aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AACACAGATGATTGCATGCCTGGGATATTCTACAg                                                                 >  2:652956/1‑93 (MQ=255)

                            aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                               >  1:1702908/1‑93 (MQ=255)

                             aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaa                                                              >  1:4596215/1‑93 (MQ=255)

                             aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaa                                                              <  2:2657952/93‑1 (MQ=255)

                              tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:1369592/1‑93 (MQ=255)

                              tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:311225/1‑93 (MQ=255)

                               aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            <  1:942109/93‑1 (MQ=255)

                                aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACATaaaaaa                                                           >  1:2320990/1‑93 (MQ=255)

                                aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           <  1:1546516/93‑1 (MQ=255)

                                 aTGAATATAACTCCTTCATGGATTTCAACAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTCCAGacaaacc                                                          <  1:4209670/93‑3 (MQ=255)

                                  tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         >  2:4424417/1‑93 (MQ=255)

                                   gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                                        >  1:3381091/1‑93 (MQ=255)

                                    aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                                       >  1:3960823/1‑93 (MQ=255)

                                    aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                                       <  2:166157/93‑1 (MQ=255)

                                     atatACCTCAGTCATGGATTGCAAAAACCGTGAATGTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      <  1:1765433/93‑1 (MQ=255)

                                      tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     <  1:117293/93‑1 (MQ=255)

                                        taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   <  1:2250310/93‑1 (MQ=255)

                                         aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                                  >  1:4518799/1‑93 (MQ=255)

                                          aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  1:3818844/93‑1 (MQ=255)

                                            tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  1:1561216/1‑93 (MQ=255)

                                            tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  1:4718327/1‑93 (MQ=255)

                                             cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                              <  2:1769168/93‑1 (MQ=255)

                                             cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                              >  1:4255672/1‑93 (MQ=255)

                                             cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                              <  2:1184367/93‑1 (MQ=255)

                                                tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  2:613093/1‑93 (MQ=255)

                                                tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  1:364629/1‑93 (MQ=255)

                                                 catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                          <  1:1288737/93‑1 (MQ=255)

                                                  atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         <  2:3528973/93‑1 (MQ=255)

                                                   tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaag                                        >  2:1250716/1‑92 (MQ=255)

                                                   tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        <  2:947180/93‑1 (MQ=255)

                                                   tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        >  1:2787796/1‑93 (MQ=255)

                                                    ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                                       <  1:1482107/93‑1 (MQ=255)

                                                    ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                                       >  2:4724675/1‑93 (MQ=255)

                                                    ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                                       <  2:1930823/93‑1 (MQ=255)

                                                     gATTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                                      >  1:2664245/1‑93 (MQ=255)

                                                      aTTTCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                                     <  1:2084747/93‑1 (MQ=255)

                                                       tttCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                                    <  1:3602006/93‑1 (MQ=255)

                                                        ttCAAAAACCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                                   <  1:926656/93‑1 (MQ=255)

                                                           aaaaaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                <  2:4060361/93‑1 (MQ=255)

                                                           aaaaaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                <  2:3808761/93‑1 (MQ=255)

                                                             aaaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              <  2:2022495/93‑1 (MQ=255)

                                                             aaaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              >  2:4105559/1‑93 (MQ=255)

                                                              aaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             <  1:564896/93‑1 (MQ=255)

                                                              aaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             >  2:3729813/1‑93 (MQ=255)

                                                              aaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             <  1:3303373/93‑1 (MQ=255)

                                                              aaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             >  1:4143338/1‑93 (MQ=255)

                                                              aaCCGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             <  2:2393929/93‑1 (MQ=255)

                                                                ccGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                           >  2:4138503/1‑93 (MQ=255)

                                                                ccGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATGCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                           <  1:61074/93‑1 (MQ=255)

                                                                 cGTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                          >  1:2287577/1‑93 (MQ=255)

                                                                  gTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata                         >  1:246366/1‑93 (MQ=255)

                                                                  gTGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata                         >  1:3219550/1‑93 (MQ=255)

                                                                   tGAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        <  2:2470030/93‑1 (MQ=255)

                                                                    gAATTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAtt                       <  1:3726153/93‑1 (MQ=255)

                                                                     aaTTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc                      >  1:2565789/1‑93 (MQ=255)

                                                                      aTTTCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa                     <  2:3451477/93‑1 (MQ=255)

                                                                       tttCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc                    <  1:3434243/93‑1 (MQ=255)

                                                                       tttCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc                    <  1:2391765/93‑1 (MQ=255)

                                                                         tCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGGTATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  >  2:2312822/1‑93 (MQ=255)

                                                                         tCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  <  2:3059617/93‑1 (MQ=255)

                                                                         tCCGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  <  1:2103486/93‑1 (MQ=255)

                                                                          ccGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg                 <  2:771510/93‑1 (MQ=255)

                                                                           cGTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg                >  2:4300688/1‑93 (MQ=255)

                                                                            gTTAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               <  2:2901515/93‑1 (MQ=255)

                                                                             ttAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg              >  2:3378312/1‑93 (MQ=255)

                                                                              tAGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa             <  2:3026144/93‑1 (MQ=255)

                                                                               aGAACAAA‑ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAt            <  1:2707783/93‑1 (MQ=255)

                                                                                   caaa‑acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        >  2:2172264/3‑93 (MQ=255)

                                                                                   caaa‑acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        <  2:4618691/91‑1 (MQ=255)

                                                                                   caaa‑acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        >  2:2850649/3‑93 (MQ=255)

                                                                                    aaa‑acaGATGATTGTATGCCTGGGGTATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTg       >  1:788149/2‑93 (MQ=255)

                                                                                      a‑acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTAACCAGGCGATTGGGGag     >  2:68178/1‑92 (MQ=255)

                                                                                        acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACg    >  1:4618661/1‑93 (MQ=255)

                                                                                         caGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgt   >  2:262246/1‑93 (MQ=255)

GATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AA‑ACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435598‑4435778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`