BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,261	T→C	L159P (CTG→CCG)	yjgP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,261	0	T	C	99.4%	554.4 / ‑5.0	171	L159P (CTG→CCG)	yjgP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (78/92); total (79/92)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 174 total aligned reads displayed.

ATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGG  >  NC_000913/4484171‑4484350

 tGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGtt                                                                                        >  1:2158483/1‑93 (MQ=255)

    aGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCAt                                                                                     >  2:662959/1‑93 (MQ=255)

        ttAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGaa                                                                                 >  2:3191263/1‑93 (MQ=255)

         tAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGaaa                                                                                <  1:261884/93‑1 (MQ=255)

         tAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGaaa                                                                                <  2:2131891/93‑1 (MQ=255)

          aGCAGAAGCGAAAGGCAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAg                                                                               <  2:797222/93‑1 (MQ=255)

             aGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGt                                                                            <  2:252349/93‑1 (MQ=255)

              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                           >  2:1864199/1‑93 (MQ=255)

              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                           >  2:3522967/1‑93 (MQ=255)

              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                           <  2:566594/93‑1 (MQ=255)

              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                           >  1:2144687/1‑93 (MQ=255)

              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                           <  1:1928330/93‑1 (MQ=255)

                agcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGa                                                                         <  1:405467/93‑1 (MQ=255)

                agcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGAGTTGa                                                                         >  1:3700686/1‑93 (MQ=255)

                accgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGa                                                                         <  2:3443186/91‑1 (MQ=255)

                 gcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAc                                                                        <  2:2099324/93‑1 (MQ=255)

                 gcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAc                                                                        <  2:2676568/93‑1 (MQ=255)

                 gcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAc                                                                        >  1:3633427/1‑93 (MQ=255)

                 acgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAc                                                                        <  2:868740/92‑1 (MQ=255)

                   gaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACgg                                                                      <  2:747856/93‑1 (MQ=255)

                   gaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACgg                                                                      >  1:3995575/1‑93 (MQ=255)

                    aaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGc                                                                     >  1:915824/1‑93 (MQ=255)

                     aagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCa                                                                    >  2:386199/1‑93 (MQ=255)

                      agcgacCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAg                                                                   <  2:2034702/93‑1 (MQ=255)

                      agcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAg                                                                   <  2:4744038/93‑1 (MQ=255)

                      agcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAg                                                                   <  2:232804/93‑1 (MQ=255)

                       gcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGc                                                                  <  1:3749754/93‑1 (MQ=255)

                         gaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGa                                                                <  1:4460327/93‑1 (MQ=255)

                             ccTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGTCAGCGATTTc                                                            >  2:665132/1‑93 (MQ=255)

                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                           >  1:341363/1‑93 (MQ=255)

                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                           >  2:4408964/1‑93 (MQ=255)

                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                           <  1:1615396/93‑1 (MQ=255)

                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                           >  2:2823510/1‑93 (MQ=255)

                              cTGGCATGGCGGCGCTGGCCCAAGGGCAATTCCATCAATCCACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                           <  1:1615395/93‑1 (MQ=255)

                                ggCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCaaa                                                         <  2:874790/93‑1 (MQ=255)

                                ggCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCaaa                                                         >  1:4353023/1‑93 (MQ=255)

                                ggCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCaaa                                                         <  2:406148/93‑1 (MQ=255)

                                   aTGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAt                                                      >  2:2533433/1‑93 (MQ=255)

                                   aTGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAt                                                      <  2:149835/93‑1 (MQ=255)

                                    tGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                     >  1:4742775/1‑93 (MQ=255)

                                    tGGCGGCGCTGGCGCAAGGGCAATTACAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                     <  2:2167851/93‑1 (MQ=255)

                                     ggcggcGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtgt                                                    <  1:1341773/93‑1 (MQ=255)

                                       cggctCTGGCGCCAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATgtgt                                                  <  1:3260321/93‑1 (MQ=255)

                                       cggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATgtgt                                                  <  2:2397075/93‑1 (MQ=255)

                                       cggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATgtgt                                                  >  1:1550543/1‑93 (MQ=255)

                                        ggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGCCAGCGTTTGCAAAGATGTGtt                                                 >  1:3871481/1‑93 (MQ=255)

                                        ggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGtt                                                 <  2:1862226/93‑1 (MQ=255)

                                           gcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCt                                              >  1:4625537/1‑93 (MQ=255)

                                           gcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCt                                              <  2:2201045/93‑1 (MQ=255)

                                           gcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCt                                              >  1:4448779/1‑93 (MQ=255)

                                            cTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTc                                             <  1:3235007/93‑1 (MQ=255)

                                            cTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTc                                             >  1:129610/1‑93 (MQ=255)

                                             tGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcg                                            >  2:1700957/1‑93 (MQ=255)

                                              ggCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcgc                                           <  2:3151581/93‑1 (MQ=255)

                                              ggCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcgc                                           <  2:3151720/93‑1 (MQ=255)

                                              ggCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcgc                                           >  2:823693/1‑93 (MQ=255)

                                               gcgcAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcgcg                                          >  2:3900268/1‑93 (MQ=255)

                                                   aaGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCaaa                                      <  1:347313/93‑1 (MQ=255)

                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCACAt                                     >  2:3496027/1‑93 (MQ=255)

                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                     <  1:11862/93‑1 (MQ=255)

                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                     >  2:1445346/1‑93 (MQ=255)

                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTACATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                     <  1:1857366/93‑1 (MQ=255)

                                                    aGGACAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                     <  2:2839672/93‑1 (MQ=255)

                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                    <  2:1418623/93‑1 (MQ=255)

                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                    >  1:2556292/1‑93 (MQ=255)

                                                        cAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGa                                 <  2:1300735/93‑1 (MQ=255)

                                                        cAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGa                                 <  2:4249863/93‑1 (MQ=255)

                                                         aaTTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGAc                                <  2:680825/93‑1 (MQ=255)

                                                         aaTTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCCAGCAAAATCGAc                                >  2:869325/1‑93 (MQ=255)

                                                          aTTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGAcc                               <  2:1082365/93‑1 (MQ=255)

                                                            tCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaa                             >  1:1007236/1‑93 (MQ=255)

                                                              cAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaaa                           >  2:1747638/1‑93 (MQ=255)

                                                              cAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaaa                           <  1:786179/93‑1 (MQ=255)

                                                               aGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAg                          >  2:282756/1‑93 (MQ=255)

                                                               aGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAg                          >  1:2997515/1‑93 (MQ=255)

                                                                gCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAgg                         <  2:586873/93‑1 (MQ=255)

                                                                 gaaGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGt                        <  2:1146784/92‑1 (MQ=255)

                                                                 cAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGt                        <  2:2208283/93‑1 (MQ=255)

                                                                  aaGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTa                       <  1:1291417/93‑1 (MQ=255)

                                                                  aaGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTa                       >  1:1127198/1‑93 (MQ=255)

                                                                  aaGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTa                       >  1:613155/1‑93 (MQ=255)

                                                                        cTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAc                 <  1:4678806/93‑1 (MQ=255)

                                                                          aaTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGt               >  1:72212/1‑93 (MQ=255)

                                                                          aaTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGt               <  2:2599611/93‑1 (MQ=255)

                                                                          aaTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGt               >  1:2094427/1‑93 (MQ=255)

                                                                            tGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTcc             >  2:1766171/1‑93 (MQ=255)

                                                                            tGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTcc             >  1:353809/1‑93 (MQ=255)

                                                                            tGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTcc             <  2:1417611/93‑1 (MQ=255)

                                                                            tGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTcc             <  1:3941323/93‑1 (MQ=255)

                                                                             ggCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCt            >  2:2322802/1‑93 (MQ=255)

                                                                             ggCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCt            >  2:2322801/1‑93 (MQ=255)

                                                                              gCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCtt           >  1:2069437/1‑93 (MQ=255)

                                                                                aGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCt         <  2:535998/93‑1 (MQ=255)

                                                                                aGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCt         <  2:4415510/93‑1 (MQ=255)

                                                                                  cTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCtgt       <  1:3027425/93‑1 (MQ=255)

                                                                                   tCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCtgtg      >  1:4186030/1‑93 (MQ=255)

                                                                                    cGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtgg     >  2:3740626/1‑93 (MQ=255)

                                                                                     ggTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggt    <  2:2971412/93‑1 (MQ=255)

                                                                                      gTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggtg   <  2:510461/93‑1 (MQ=255)

                                                                                      gTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggtg   >  1:4282608/1‑93 (MQ=255)

ATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGG  >  NC_000913/4484171‑4484350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`