BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,644	A→G	W15R (TGG→CGG)	lplA ←	lipoate‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,644	0	A	G	98.8%	541.6 / ‑3.0	170	W15R (TGG→CGG)	lplA	lipoate‑protein ligase A
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (67/101); total (67/103)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 181 total aligned reads displayed.

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         >  1:722266/1‑93 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         <  1:1583226/93‑1 (MQ=255)

 tacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCgg                                                                                        <  1:4439943/93‑1 (MQ=255)

    cGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTc                                                                                     <  2:4325801/93‑1 (MQ=255)

    cGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTc                                                                                     <  1:4017832/93‑1 (MQ=255)

     gtgtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCg                                                                                    >  1:3866395/1‑93 (MQ=255)

        tCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTaa                                                                                 >  2:2141137/1‑93 (MQ=255)

        tCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTaa                                                                                 >  1:2958764/1‑93 (MQ=255)

           gCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAagag                                                                              >  2:1888031/1‑93 (MQ=255)

            cATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGt                                                                             >  1:3068983/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  1:722147/93‑1 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  2:1394548/93‑1 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  2:3080231/93‑1 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  2:8094/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  1:1091275/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAAACGCGGGTCGTAagaggg                                                                            >  2:3471749/1‑91 (MQ=255)

               tGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCag                                                                          >  2:2663107/1‑93 (MQ=255)

               tGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCag                                                                          <  1:2129792/93‑1 (MQ=255)

                  gcaagagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAgaga                                                                       <  1:4289390/90‑1 (MQ=255)

                  gcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAgaga                                                                       <  1:3310841/93‑1 (MQ=255)

                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      <  1:3287386/93‑1 (MQ=255)

                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      >  2:3787949/1‑93 (MQ=255)

                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      >  1:3398470/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  1:357403/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  2:3656782/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  1:531992/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  2:110711/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     <  2:749551/93‑1 (MQ=255)

                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    >  2:2059793/1‑93 (MQ=255)

                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    <  2:2294734/93‑1 (MQ=255)

                      gagaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAg                                                                   <  1:746112/93‑1 (MQ=255)

                      gagaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAg                                                                   <  2:847077/93‑1 (MQ=255)

                      gagaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAg                                                                   >  2:3680372/1‑93 (MQ=255)

                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  2:3272811/93‑1 (MQ=255)

                          aaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAgg                                                               >  1:4077912/1‑93 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              >  1:3327825/1‑93 (MQ=255)

                            cAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCg                                                             >  1:2424719/1‑93 (MQ=255)

                             aGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                            >  2:1512455/1‑93 (MQ=255)

                             aGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                            <  2:3806967/93‑1 (MQ=255)

                              gAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                           >  1:3480233/1‑93 (MQ=255)

                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         <  2:272955/93‑1 (MQ=255)

                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         <  1:3810695/93‑1 (MQ=255)

                                 cgcgCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                        <  1:150879/93‑1 (MQ=255)

                                    gcTGCGTGGCGGGCATTTGGCGAAAAATACAATCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTgg                                                     >  2:1653858/1‑93 (MQ=255)

                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTTAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    >  2:1460041/1‑93 (MQ=255)

                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCTGAATGTGGa                                                    >  2:4023509/1‑93 (MQ=255)

                                         gTGGCGGGCATTTGGCGAAAAATACCCTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATa                                                <  2:2381232/93‑1 (MQ=255)

                                         gTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATa                                                <  1:202605/93‑1 (MQ=255)

                                           ggCGGGCATTTGGCGAAAAATACACTCTTCCCCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAc                                              <  1:2838861/93‑1 (MQ=255)

                                            gCGGGCAGTGGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACg                                             <  1:851412/93‑1 (MQ=255)

                                               gtgaTTTGGCGAAAAATTCACCCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          <  2:2249441/90‑1 (MQ=255)

                                               gtcATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          <  1:2014677/91‑1 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          <  2:2667007/93‑1 (MQ=255)

                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                         >  2:56425/1‑93 (MQ=255)

                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                         >  2:3736571/1‑93 (MQ=255)

                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                         <  1:2446774/93‑1 (MQ=255)

                                                   tttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCt                                      <  2:4284389/93‑1 (MQ=255)

                                                   tttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCt                                      <  1:3611953/93‑1 (MQ=255)

                                                    ttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                     <  2:2700152/93‑1 (MQ=255)

                                                     tGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                    <  2:3261965/93‑1 (MQ=255)

                                                       gCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCt                                  <  2:3695726/93‑1 (MQ=255)

                                                       gCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCt                                  <  1:2589194/93‑1 (MQ=255)

                                                       gCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCt                                  <  1:4239686/93‑1 (MQ=255)

                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 <  1:3280774/93‑1 (MQ=255)

                                                         gAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTg                                >  1:924320/1‑93 (MQ=255)

                                                         gAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTg                                <  2:2773550/93‑1 (MQ=255)

                                                          aaaaaTTCCCTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgt                               <  2:3591626/93‑1 (MQ=255)

                                                            aaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag                             <  1:1415285/93‑1 (MQ=255)

                                                             aaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                            <  2:4665367/93‑1 (MQ=255)

                                                             aaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                            >  1:3225905/1‑93 (MQ=255)

                                                             aaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                            <  2:28307/93‑1 (MQ=255)

                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           >  1:1062978/1‑93 (MQ=255)

                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           >  1:1062977/1‑93 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:2758203/1‑93 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:2131982/1‑93 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          <  1:2854337/93‑1 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:2758202/1‑93 (MQ=255)

                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGGCGTAAGAGTCAGAGATGAGCAGGCGAAATGTGGACATAACGATTACTTTTCTGGTAGTAAt                         >  1:3199118/1‑93 (MQ=255)

                                                                  acTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATgg                       <  1:2765123/93‑1 (MQ=255)

                                                                    tctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGt                     <  1:291300/93‑1 (MQ=255)

                                                                    tctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGt                     >  1:1910315/1‑93 (MQ=255)

                                                                    tctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGt                     >  1:4537135/1‑93 (MQ=255)

                                                                      ttCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCa                   >  2:4656501/1‑93 (MQ=255)

                                                                       tCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAc                  <  2:2464946/93‑1 (MQ=255)

                                                                        ccACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                 >  1:480122/1‑93 (MQ=255)

                                                                        ccACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                 >  1:1741889/1‑93 (MQ=255)

                                                                        ccACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                 <  2:4378608/93‑1 (MQ=255)

                                                                        ccACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                 >  1:401823/1‑93 (MQ=255)

                                                                          aCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct               >  1:4631595/1‑93 (MQ=255)

                                                                            cGCCAGGTTAAAGCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttt             <  2:1580357/93‑1 (MQ=255)

                                                                                aGGTTAAACCGCAGGTCGTAAGAGTCCGAGATGAGCAGGCGCAATGTGGACATAACGATTTCCTTTCTTGTAGGAATGCGTCACTCTTTTTtc         >  2:3252997/1‑93 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtct        <  2:3172297/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGAGTCGTAAGAGTCAGAGAAGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:665041/93‑1 (MQ=255)

                                                                                    tAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGc     <  1:3576908/93‑1 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  1:2575545/93‑1 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    >  1:185074/1‑93 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  2:2653207/93‑1 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  1:1203914/93‑1 (MQ=255)

                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   <  1:952891/93‑1 (MQ=255)

                                                                                       aCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt  <  2:2532771/93‑1 (MQ=255)

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`