BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,914	C→T	G365S (GGC→AGC)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,914	0	C	T	99.3%	501.8 / ‑5.5	143	G365S (GGC→AGC)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (77/65); total (78/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 149 total aligned reads displayed.

CCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672824‑673003

ccGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGt                                                                                         <  1:1751472/93‑1 (MQ=255)

 cGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTc                                                                                        <  1:335438/93‑1 (MQ=255)

  gCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCa                                                                                       >  1:70818/1‑93 (MQ=255)

    ttCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     <  1:1109772/93‑1 (MQ=255)

    ttCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     >  1:1167195/1‑93 (MQ=255)

    ttCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     >  1:2544568/1‑93 (MQ=255)

     tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    >  1:865728/1‑93 (MQ=255)

      cATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTg                                                                                   >  2:3500819/1‑93 (MQ=255)

       aTGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGc                                                                                  <  1:3732711/93‑1 (MQ=255)

        tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 >  2:2619561/1‑93 (MQ=255)

        tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 >  1:4383472/1‑93 (MQ=255)

        tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 <  1:976140/93‑1 (MQ=255)

           tCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAgg                                                                              <  1:3628002/93‑1 (MQ=255)

           tCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAgg                                                                              >  2:3680867/1‑93 (MQ=255)

            cAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGa                                                                             <  2:591885/93‑1 (MQ=255)

             aaGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGAt                                                                            >  2:3268866/1‑93 (MQ=255)

             aaGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGAt                                                                            >  1:3359120/1‑93 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  1:2002461/1‑93 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  2:4447257/1‑93 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  1:2942843/1‑93 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  2:2941035/1‑93 (MQ=255)

               gACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATaa                                                                          >  2:683250/1‑93 (MQ=255)

                aCCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAc                                                                         <  1:4335890/93‑1 (MQ=255)

                aCCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAc                                                                         >  2:1304748/1‑93 (MQ=255)

                aCCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAc                                                                         <  1:3243570/93‑1 (MQ=255)

                aCCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAc                                                                         <  2:2370732/93‑1 (MQ=255)

                 ctgatGAACTCGCCATAGTTCAACAGCACGCATTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTGAGAGCTGTCAGCTGCCAGGATAAcc                                                                        <  2:3293484/89‑1 (MQ=255)

                 ccGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAcc                                                                        >  1:2771937/1‑93 (MQ=255)

                  cGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCCGTCAGGGCTTGCTGAGAGAGATCTGGCTCAGAGGTGTCAGCTGCCAGGCTAAcag                                                                       >  2:1736892/1‑91 (MQ=255)

                  cGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCg                                                                       >  2:1736891/1‑93 (MQ=255)

                   gTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCgg                                                                      >  2:1397642/1‑93 (MQ=255)

                    ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  2:3139973/1‑93 (MQ=255)

                    ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  2:3139974/1‑93 (MQ=255)

                    ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  1:2097342/1‑93 (MQ=255)

                       accTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTg                                                                  <  2:2553817/91‑1 (MQ=255)

                       aaCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTg                                                                  >  1:1637561/1‑93 (MQ=255)

                        aCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGa                                                                 <  2:4238841/93‑1 (MQ=255)

                        aCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGa                                                                 <  1:4180676/93‑1 (MQ=255)

                          tCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATg                                                               >  1:542201/1‑93 (MQ=255)

                            gCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGtt                                                             <  2:1033620/93‑1 (MQ=255)

                            gCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGtt                                                             >  1:975062/1‑93 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            >  2:937136/1‑93 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            >  1:3270503/1‑93 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            <  1:3927505/93‑1 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAAACGGTTTGATGTTc                                                            >  1:3160850/1‑93 (MQ=255)

                              cAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           >  1:2404652/1‑93 (MQ=255)

                               agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          >  2:4145035/1‑93 (MQ=255)

                               agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          >  2:284463/1‑93 (MQ=255)

                                gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  1:3126528/1‑93 (MQ=255)

                                gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  2:3385120/1‑93 (MQ=255)

                                  gTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGcc                                                       >  2:3753403/1‑93 (MQ=255)

                                   ttGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCg                                                      <  2:1690824/93‑1 (MQ=255)

                                     gAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTa                                                    >  1:1417822/1‑93 (MQ=255)

                                      aaCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAt                                                   <  1:4563286/93‑1 (MQ=255)

                                       aCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAtt                                                  <  1:391558/93‑1 (MQ=255)

                                            aCGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTagag                                             >  2:1317250/1‑93 (MQ=255)

                                                cTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaa                                         <  2:2005843/93‑1 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:329293/1‑93 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  2:295410/1‑93 (MQ=255)

                                                      cagtcagGGCTGGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGt                                   <  2:4118608/93‑1 (MQ=255)

                                                       agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  >  1:1298897/1‑93 (MQ=255)

                                                       agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:111021/93‑1 (MQ=255)

                                                       agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  1:4510020/93‑1 (MQ=255)

                                                       agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:2701474/93‑1 (MQ=255)

                                                        gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 >  2:3932197/1‑93 (MQ=255)

                                                        gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 >  2:531501/1‑93 (MQ=255)

                                                        gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 >  2:3546273/1‑93 (MQ=255)

                                                        gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 >  2:3932195/1‑93 (MQ=255)

                                                         ccagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGt                                <  2:2225626/92‑1 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              >  2:1931466/1‑93 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              >  1:3025399/1‑93 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              >  1:3570851/1‑93 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              >  1:1913926/1‑93 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             >  1:1263411/1‑93 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             <  1:2198179/93‑1 (MQ=255)

                                                               cTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCt                          <  1:3555869/93‑1 (MQ=255)

                                                                ttGCAGAGAAAGAGCTGGCTCACAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCtc                         <  2:2418617/93‑2 (MQ=255)

                                                                 tGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTgg                        <  1:3783825/93‑1 (MQ=255)

                                                                   gtGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTc                      <  1:3816412/92‑1 (MQ=255)

                                                                   cTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTc                      <  1:772466/93‑1 (MQ=255)

                                                                    tGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCg                     >  1:3833391/1‑93 (MQ=255)

                                                                     gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    >  2:58154/1‑93 (MQ=255)

                                                                     gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    >  2:3561045/1‑93 (MQ=255)

                                                                     gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    >  1:2301243/1‑93 (MQ=255)

                                                                     gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    <  1:3483656/93‑1 (MQ=255)

                                                                     gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGAAGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    >  1:3663244/1‑93 (MQ=255)

                                                                      agaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTg                   <  1:4123890/93‑1 (MQ=255)

                                                                       gaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGc                  <  2:883713/93‑1 (MQ=255)

                                                                        aaaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcc                 <  1:3303454/93‑1 (MQ=255)

                                                                         aaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGccc                <  1:1033076/93‑1 (MQ=255)

                                                                          aGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcccc               <  1:1468941/93‑1 (MQ=255)

                                                                          aGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcccc               <  2:2275365/93‑1 (MQ=255)

                                                                          aGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcccc               <  2:629217/93‑1 (MQ=255)

                                                                             tCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGt            <  2:1575710/93‑1 (MQ=255)

                                                                               tGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAc          >  1:2838545/1‑93 (MQ=255)

                                                                                ggCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAcc         <  2:1452433/93‑1 (MQ=255)

                                                                                    cAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCa     >  1:1748203/1‑93 (MQ=255)

                                                                                     agagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCAt    >  2:714275/1‑93 (MQ=255)

                                                                                      gagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCCAACTCGTAGTCGCGCTGCTCGTGCCCCGGCACCGCCATa   >  1:3969719/1‑93 (MQ=255)

                                                                                      gagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATa   <  1:4684635/93‑1 (MQ=255)

CCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672824‑673003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`