Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 766,372 C→T A389V (GCG→GTG)  hrsA → PTS system fructose specific IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,3720CT99.0% 349.3 / NA 102A389V (GCG→GTG) hrsAPTS system fructose specific IIABC component
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (53/47);  minor base G (0/1);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG  >  NC_000913/766284‑766460
                                                                                        |                                                                                        
gggCTGCTCGGAAATTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCt                                                                                      <  1:2829068/93‑1 (MQ=255)
gggCCGCTTGTAATTTTGATGGTACCGGTGCTCGGGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCt                                                                                      <  1:2829069/93‑1 (MQ=255)
 ggatgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                     <  2:1793967/90‑1 (MQ=255)
 ggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                     >  2:968204/1‑93 (MQ=255)
 ggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                     <  2:922458/93‑1 (MQ=255)
  gctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgg                                                                                    >  1:1083222/1‑93 (MQ=255)
  gctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgg                                                                                    >  1:577630/1‑93 (MQ=255)
  gctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgg                                                                                    <  2:1120869/93‑1 (MQ=255)
    tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                  >  2:2296018/1‑93 (MQ=255)
    tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                  <  1:3000575/93‑1 (MQ=255)
      ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                >  2:3906189/1‑93 (MQ=255)
      ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                >  1:924624/1‑93 (MQ=255)
        cGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGCTTgc                                                                              >  1:4554633/1‑93 (MQ=255)
        cGGAATTTTGATGGTACCCGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAAACTGCGTTAGCGCCAGGCTTTGTGGCTGGTCTTgc                                                                              >  1:4554631/1‑93 (MQ=255)
         ggAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgcc                                                                             >  2:4389010/1‑93 (MQ=255)
           aaTTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgccgc                                                                           <  2:4136070/93‑1 (MQ=255)
              tttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCaa                                                                        <  2:4139930/93‑1 (MQ=255)
                tGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACa                                                                      <  2:4139348/93‑1 (MQ=255)
                  aTGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATg                                                                    >  2:2467778/1‑93 (MQ=255)
                   tGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGa                                                                   >  2:126852/1‑93 (MQ=255)
                   tGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGa                                                                   <  2:2127122/93‑1 (MQ=255)
                    ggTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAt                                                                  <  2:1993164/93‑1 (MQ=255)
                     gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                 <  2:3320825/93‑1 (MQ=255)
                      tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCg                                                                <  1:694491/93‑1 (MQ=255)
                       aCCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCgg                                                               >  2:104323/1‑93 (MQ=255)
                        ccGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                              <  2:2805185/93‑1 (MQ=255)
                                 gcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGttt                                                     >  2:2392985/1‑93 (MQ=255)
                                  cgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCCACATGATCGGCTCCGGGtttt                                                    >  1:3659655/1‑92 (MQ=255)
                                  cgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtc                                                    <  2:2231597/93‑1 (MQ=255)
                                   ggCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtct                                                   <  2:3911233/93‑1 (MQ=255)
                                   ggCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtct                                                   <  2:4622152/93‑1 (MQ=255)
                                    gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTTGCTTGACGGGCCGCCAACATGATCGGCTTCGGGTTtcct                                                  >  2:4199688/1‑91 (MQ=255)
                                    gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCCGCTCCGGGTTtctc                                                  >  2:2920314/1‑93 (MQ=255)
                                     ccTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCg                                                 <  2:342612/93‑1 (MQ=255)
                                     ccTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCg                                                 >  1:986345/1‑93 (MQ=255)
                                     ccTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCAAACATGATCGGCTCCGGGGTTCTCg                                                 >  1:986346/1‑93 (MQ=255)
                                      cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                <  2:155388/93‑1 (MQ=255)
                                      cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                >  1:2809325/1‑93 (MQ=255)
                                      cTATACCGCCTAGTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGGGGCTGGACTTGCCGCCAACATGATCGGCTCCTGGTTTCTCgg                                                <  2:155387/93‑1 (MQ=255)
                                       tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgc                                               <  2:3298107/93‑1 (MQ=255)
                                       tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGgc                                               >  1:412667/1‑93 (MQ=255)
                                        ataCCGCCTATTCTCTGGCAGATAAAACGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGTCAACATGATCGGCTCCGGGATTCTCGgcg                                              >  2:898027/1‑93 (MQ=255)
                                         taCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcgc                                             <  2:3383928/93‑1 (MQ=255)
                                            cGCCTATTGTCTGGCAGATAAACCGGCGTTAGCGCCAGACATTGTGGCTGGACTTGCCGCCACCATGATCGGCTCCGGGTTTCTCGGCGCGGt                                          <  2:2272912/93‑1 (MQ=255)
                                              ccTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCg                                        >  1:4136160/1‑93 (MQ=255)
                                              ccTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCg                                        >  1:1409281/1‑93 (MQ=255)
                                              ccTATTCTCGGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCg                                        <  1:2358771/93‑1 (MQ=255)
                                               cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGt                                       <  2:1655021/93‑1 (MQ=255)
                                               cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGt                                       <  2:696152/93‑1 (MQ=255)
                                               cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGt                                       <  2:2036861/93‑1 (MQ=255)
                                                tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                      >  2:401235/1‑93 (MQ=255)
                                                 aTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTg                                     <  2:1863218/93‑1 (MQ=255)
                                                 aTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTg                                     <  2:1039693/93‑1 (MQ=255)
                                                  ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                    >  1:1421739/1‑93 (MQ=255)
                                                  ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                    <  2:3357218/93‑1 (MQ=255)
                                                  ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                    >  1:361550/1‑93 (MQ=255)
                                                  ttCTCTGGCAGATAAACCGGAGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                    <  2:570745/93‑1 (MQ=255)
                                                   tctcTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGc                                   <  2:920571/93‑1 (MQ=255)
                                                    ctctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCg                                  <  2:4511975/93‑1 (MQ=255)
                                                     tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCgg                                 >  1:443035/1‑93 (MQ=255)
                                                     tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCgg                                 >  2:1252045/1‑93 (MQ=255)
                                                     tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGGCGTTGGCgg                                 >  1:2102698/1‑93 (MQ=255)
                                                     tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGCCGTTGGCgg                                 >  1:4664971/1‑93 (MQ=255)
                                                     tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGATTCTCGGCGCGGTCGGTGGCgg                                 >  2:4469270/1‑93 (MQ=255)
                                                       tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAt                               <  2:1636135/93‑1 (MQ=255)
                                                        ggCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGAtt                              >  1:1504184/1‑93 (MQ=255)
                                                          cAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGa                            >  1:1349479/1‑93 (MQ=255)
                                                           aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                           <  2:2028968/93‑1 (MQ=255)
                                                           aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGAt                           >  1:1740116/1‑93 (MQ=255)
                                                            gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                          >  1:3942124/1‑93 (MQ=255)
                                                            gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                          <  2:1891620/93‑1 (MQ=255)
                                                            gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGGTGGCGGATTGATa                          >  2:2212974/1‑93 (MQ=255)
                                                              tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                        <  1:2205629/93‑1 (MQ=255)
                                                               aaaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGcc                       >  1:4728269/1‑93 (MQ=255)
                                                               aaaCCGGCCTTAGCGCCAGGCTTTGTGGCAGGACTTGCCGCAAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGcc                       <  1:1648842/93‑1 (MQ=255)
                                                                aaCCGGCGTTAGCGCCAGGGTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTGCTCGGCGCGGTCGTTGGCGGATTGATAGCCg                      <  2:993427/93‑1 (MQ=255)
                                                                aaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCg                      >  1:3293181/1‑93 (MQ=255)
                                                                 aCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCgg                     >  1:2319659/1‑93 (MQ=255)
                                                                  ccGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGt                    <  1:4691292/93‑1 (MQ=255)
                                                                   cGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGa                            >  2:4080810/1‑84 (MQ=255)
                                                                    ggCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATGGCCGGTTa                  >  2:2131667/1‑93 (MQ=255)
                                                                    ggCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTa                  >  2:1005953/1‑93 (MQ=255)
                                                                     gCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAc                 <  1:3941215/93‑1 (MQ=255)
                                                                     gCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAc                 >  1:325947/1‑93 (MQ=255)
                                                                       gTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACtt               >  2:4075390/1‑93 (MQ=255)
                                                                        ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg              >  2:2479941/1‑93 (MQ=255)
                                                                        ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg              >  1:35728/1‑93 (MQ=255)
                                                                        ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg              <  2:4688852/93‑1 (MQ=255)
                                                                        ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg              >  2:3595349/1‑93 (MQ=255)
                                                                         tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa             <  2:2702240/93‑1 (MQ=255)
                                                                          aGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAt            >  1:1590376/1‑93 (MQ=255)
                                                                          aGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAt            <  1:4291730/93‑1 (MQ=255)
                                                                             gcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGGTTGATAGCCGGTTACTTGATgcg         >  2:3391438/1‑93 (MQ=255)
                                                                             gcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcg         >  1:3467884/1‑93 (MQ=255)
                                                                              ccAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcgc        >  1:4746043/1‑93 (MQ=255)
                                                                                aGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTg      >  2:62644/1‑93 (MQ=255)
                                                                                   cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt   <  2:2977379/93‑1 (MQ=255)
                                                                                   cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt   <  1:1407396/93‑1 (MQ=255)
                                                                                   cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt   <  2:2977378/93‑1 (MQ=255)
                                                                                    tttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTg  <  2:4261622/93‑1 (MQ=255)
                                                                                        |                                                                                        
GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG  >  NC_000913/766284‑766460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑