Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 934,839 T→C V798A (GTT→GCT)  ftsK → cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913934,8390TC97.9% 283.2 / ‑3.4 97V798A (GTT→GCT) ftsKcell division protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (49/46);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

ACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATG  >  NC_000913/934750‑934928
                                                                                         |                                                                                         
acaacaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTgc                                                                                        >  1:1160316/1‑93 (MQ=255)
   acaGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGcgcc                                                                                     >  1:7411/1‑93 (MQ=255)
    caGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGcgccg                                                                                    >  2:909071/1‑93 (MQ=255)
       cgggTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcag                                                                                 <  2:3258150/91‑1 (MQ=255)
         ggTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagca                                                                               >  2:1041941/1‑93 (MQ=255)
         ggTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGGTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagca                                                                               >  2:197609/1‑93 (MQ=255)
          gTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcag                                                                              >  2:2199418/1‑93 (MQ=255)
          gTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcag                                                                              >  1:2997912/1‑93 (MQ=255)
           ttGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcagc                                                                             >  2:2762353/1‑93 (MQ=255)
            tGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAAAAACCGGCTGCGCCgcagcagca                                                                            <  1:3403529/93‑1 (MQ=255)
            tGCGCCGCAGCAACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCgcagcagca                                                                            <  2:4178598/93‑1 (MQ=255)
               gccgcAGCCACAAGATCAGCCGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTa                                                                         <  2:3524502/93‑1 (MQ=255)
               cccgcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTa                                                                         <  2:2430927/92‑1 (MQ=255)
                ccgcAGCAACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTAt                                                                        <  2:602430/93‑1 (MQ=255)
                 cgcAGCAACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATc                                                                       <  1:3193317/93‑1 (MQ=255)
                  gcAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                      >  2:1906977/1‑93 (MQ=255)
                  cccaccACAATTCCAGCAGCCGCAACAACCGCTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATca                                                                      <  1:259110/89‑1 (MQ=17)
                    acccACAATATCAGCAGCCGCAACAGCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagc                                                                    <  2:4294317/91‑1 (MQ=255)
                    aGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagc                                                                    >  1:4683146/1‑93 (MQ=255)
                    aGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagc                                                                    >  2:3898577/1‑93 (MQ=255)
                    aGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCAGCTGCGCCGCAGCAGCAGTATcagc                                                                    >  1:871481/1‑93 (MQ=255)
                    aGCCACAATATCAACAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagc                                                                    >  1:871482/1‑93 (MQ=255)
                     gCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagca                                                                   >  2:2639772/1‑93 (MQ=255)
                     gCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagca                                                                   >  1:3180467/1‑93 (MQ=255)
                     gCCACAATACCACCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATcagca                                                                   <  2:67262/93‑1 (MQ=255)
                          aaTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                              >  2:198862/1‑93 (MQ=255)
                          aaTATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCcac                                                              <  1:1376974/93‑1 (MQ=255)
                            tatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                            <  1:4384525/93‑1 (MQ=255)
                            tatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                            >  2:1774727/1‑93 (MQ=255)
                            tatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                            >  2:3007434/1‑93 (MQ=255)
                            gatCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                            <  2:242635/92‑1 (MQ=255)
                            aatCACCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaa                                                            <  1:2053653/92‑1 (MQ=255)
                             atCAGCAGCCGCACCAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCacaac                                                           <  1:1020340/93‑1 (MQ=255)
                               caacagcCGCAACAACGCGTTGCCCCACAGCAGCAATATCAGCAGCCGCAACAACCCGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAg                                                         <  1:2671589/90‑1 (MQ=14)
                                agcagcCGCACCACCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACATCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGc                                                        <  2:2973490/93‑1 (MQ=17)
                                 gcagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGcc                                                       <  2:1351983/93‑1 (MQ=255)
                                 gcagcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGcc                                                       >  2:3617974/1‑93 (MQ=255)
                                  cagcCGCAACACCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCa                                                      <  2:1500966/93‑1 (MQ=255)
                                    gcCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGt                                                    <  1:1351979/93‑1 (MQ=255)
                                    cccGCAACAACCGATTGCCCACCAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGt                                                    <  1:1351980/92‑1 (MQ=14)
                                     ccGCAACACCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                   <  2:2351877/93‑1 (MQ=255)
                                     ccGCAACAACCTGTTGCGCCACACAAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                   <  2:2626429/93‑1 (MQ=17)
                                     ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                   <  1:4638655/93‑1 (MQ=255)
                                     ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                   <  1:2974730/93‑1 (MQ=255)
                                     ccGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGtt                                                   <  2:795706/93‑1 (MQ=255)
                                        caacaaCCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgcg                                                >  2:2837030/1‑93 (MQ=255)
                                         aacaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgcgc                                               >  2:4035283/1‑93 (MQ=255)
                                         aacaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTgcgc                                               >  2:873448/1‑93 (MQ=255)
                                          acaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACACCAGCCAGTTGCGcc                                              >  1:1133494/1‑93 (MQ=255)
                                          acaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGcc                                              >  1:2275833/1‑93 (MQ=255)
                                          acaacCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGcc                                              >  2:1715761/1‑93 (MQ=255)
                                             acCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCcaca                                           >  2:4297669/1‑93 (MQ=255)
                                              ccGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACaa                                          >  1:1702679/1‑93 (MQ=255)
                                               cGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAc                                         <  1:996554/93‑1 (MQ=255)
                                                 gtcgcgcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                       <  2:323870/90‑1 (MQ=255)
                                                 gatGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGACAGTTGCGCCACAACCg                                       >  2:3992381/3‑93 (MQ=255)
                                                 gTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCg                                       <  1:1733255/93‑1 (MQ=255)
                                                   tGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCa                                     <  1:807684/93‑1 (MQ=255)
                                                   tGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCa                                     <  2:4428358/93‑1 (MQ=255)
                                                    tcgcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAg                                    <  2:1682170/92‑1 (MQ=255)
                                                     cgcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAgg                                   >  2:2201637/1‑93 (MQ=255)
                                                      gcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCCGGa                                  >  1:3195678/1‑93 (MQ=255)
                                                      gcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGa                                  >  1:1823170/1‑93 (MQ=255)
                                                      gcCACAGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAAACGCAGGa                                  >  2:4071052/1‑93 (MQ=255)
                                                        cacaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATa                                >  1:1485108/1‑93 (MQ=255)
                                                         ccagcagCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                               <  1:3096469/92‑1 (MQ=255)
                                                         acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACACCAGCCAGCTCTGCCACAACCGCAGGATAc                               >  1:4234441/1‑93 (MQ=18)
                                                         acaGCAGCAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAc                               >  2:2354890/1‑93 (MQ=255)
                                                           agcagcAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                             >  2:3284886/1‑93 (MQ=255)
                                                           agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                             <  2:1755827/93‑1 (MQ=255)
                                                           agcagcAATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATAccc                             <  2:1547446/93‑1 (MQ=255)
                                                            gcagcaATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCt                            <  1:3494866/93‑1 (MQ=255)
                                                              agcaATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGc                          >  1:152886/1‑93 (MQ=255)
                                                              agcaATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGc                          >  2:683606/1‑93 (MQ=255)
                                                                caATATCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCtt                        <  2:4382959/93‑1 (MQ=255)
                                                                   tatCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCAt                     >  2:3590001/1‑93 (MQ=255)
                                                                     tCAGCAGCCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATcc                   >  2:3631074/1‑93 (MQ=255)
                                                                        gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                >  2:1237417/1‑93 (MQ=255)
                                                                        gcagcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCt                >  2:3295086/1‑93 (MQ=255)
                                                                          agcCGCAACAACCGGCTGCGTCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTTCTTCATCCCCTGt              >  2:1288138/1‑93 (MQ=25)
                                                                          agcCGCAACAACCGGCTGCGCCTCAGCAGCAATATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt              >  1:845940/1‑93 (MQ=25)
                                                                          agcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt              <  1:2985290/93‑1 (MQ=255)
                                                                          agcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGt              >  2:2359142/1‑93 (MQ=255)
                                                                           gcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt             <  1:2337387/93‑1 (MQ=255)
                                                                           gcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt             <  1:1683164/93‑1 (MQ=255)
                                                                           gcCGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGtt             >  1:1862755/1‑93 (MQ=255)
                                                                            ccGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGCTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTg            >  1:1535871/1‑93 (MQ=255)
                                                                             cGCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGa           <  1:3003069/93‑1 (MQ=255)
                                                                              gCAACAACCGGCTGCGCCGCAGCGGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt          >  1:4183402/1‑93 (MQ=255)
                                                                              gCAACAACCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt          <  1:1171590/93‑1 (MQ=255)
                                                                              ccaacaaCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGAt          <  1:1551823/92‑1 (MQ=255)
                                                                               caacaaCCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATg         <  1:2871551/93‑1 (MQ=255)
                                                                                  caacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGt      <  1:725376/93‑1 (MQ=255)
                                                                                  caacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGt      <  1:4714178/93‑1 (MQ=255)
                                                                                   aacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa     >  2:454533/1‑93 (MQ=255)
                                                                                   aacCGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTa     >  2:3658258/1‑93 (MQ=255)
                                                                                   aacCGGCTGCGCCGCAGCAGCAGCATCAGCAGCCCCAACGGCCAGTTGCGCCACAACCGCAGGATAACCTGCTTCCTCCGCTGTTGATGCGTa     >  1:2675566/1‑93 (MQ=255)
                                                                                      cGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATg  <  2:1689312/93‑1 (MQ=255)
                                                                                      cGGCTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATg  >  1:1169626/1‑93 (MQ=255)
                                                                                         |                                                                                         
ACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCGCCGCAGCAGCAGTATCAGCAGCCACAACAGCCAGTTGCGCCACAACCGCAGGATACCCTGCTTCATCCGCTGTTGATGCGTAATG  >  NC_000913/934750‑934928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: