BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,364	A→G	I275V (ATT→GTT)	mukF →	MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,364	0	A	G	98.8%	511.7 / ‑2.9	163	I275V (ATT→GTT)	mukF	MukF
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (91/70); total (93/70)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 169 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                         <  1:152326/93‑1 (MQ=255)

gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGACCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                         >  2:1509278/1‑93 (MQ=255)

gATCTGCATTTCGTCCATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                         <  1:3640175/93‑1 (MQ=255)

 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        >  1:2420561/1‑93 (MQ=255)

   ttGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      <  2:1416295/92‑1 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      >  1:2091606/1‑93 (MQ=255)

       aTTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACg                                                                                  >  2:2665268/1‑93 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACgc                                                                                 >  2:3768769/1‑92 (MQ=255)

         ttCGTCGATCGTCTTGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                <  1:4021975/93‑1 (MQ=255)

              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGGAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                           >  2:3938270/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                         >  1:4572548/1‑93 (MQ=255)

                 tCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGt                                                                        <  2:689888/93‑1 (MQ=255)

                   gTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTac                                                                      >  1:2941259/1‑93 (MQ=255)

                    tCTGGTGTTCGCTCTGCAGAGCCAACTCGATCGTATTATCCGTTGGGGCCAGAAAGCCATCGACTTCTGGGCTGGGTCTGCCGGCCACGTaac                                                                     >  1:4346431/1‑91 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                   <  2:4654684/93‑1 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                   >  1:4658039/1‑93 (MQ=255)

                       ggTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACaa                                                                  <  2:683861/93‑1 (MQ=255)

                        gtgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACaaa                                                                 >  1:2923807/1‑93 (MQ=255)

                        gtgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGCCCAGAAATCCATCGACTTGTGTGTTGGCTACGACCGCCACGTACACaaa                                                                 <  2:525946/93‑1 (MQ=255)

                         tgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAt                                                                <  1:692989/93‑1 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               >  2:557613/1‑93 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               <  2:1544526/93‑1 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              >  2:2009338/1‑93 (MQ=255)

                             cGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAt                                                            >  1:3914143/1‑93 (MQ=255)

                              gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAtt                                                           <  2:3079588/93‑1 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          <  2:4266237/93‑1 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          >  1:3714842/1‑93 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          >  1:3748361/1‑93 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGACACGTACACAAATTTATTc                                                          >  2:2005115/1‑93 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATTGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         >  2:1305991/1‑93 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         >  1:3089317/1‑93 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         >  2:1929924/1‑93 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  2:3455316/1‑93 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  1:3968894/1‑93 (MQ=255)

                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                      <  2:1669015/93‑1 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                    >  1:873177/1‑93 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   <  2:3247668/93‑1 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   <  1:2715390/93‑1 (MQ=255)

                                       agCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgcg                                                  <  1:3483234/93‑1 (MQ=255)

                                       agCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTCGCTACGACCGCCACGTACACAAATTTATTCGTACcgcg                                                  >  2:2142716/1‑93 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                <  1:1144168/93‑1 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                <  2:2721217/93‑1 (MQ=255)

                                           aaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcg                                              >  2:2158568/1‑93 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcgt                                             >  2:1104832/1‑92 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                             >  1:3409183/1‑93 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                             >  1:2248308/1‑93 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                             <  2:2037172/93‑1 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           >  2:4547203/1‑93 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           >  2:373491/1‑93 (MQ=255)

                                               cGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGatat                                          >  2:1644201/1‑93 (MQ=255)

                                               cGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGatat                                          <  2:1130921/93‑1 (MQ=255)

                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         >  1:2441343/1‑93 (MQ=255)

                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         <  2:2166032/93‑1 (MQ=255)

                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         >  2:1476480/1‑93 (MQ=255)

                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                        >  1:1691059/1‑93 (MQ=255)

                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                       <  1:1943436/93‑1 (MQ=255)

                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                       >  1:4676117/1‑93 (MQ=255)

                                                  acGTATTATCAGTTGGGGCCAGCAATACATCGACGTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAACGCGATCGATAGGGa                                       <  2:4346431/92‑1 (MQ=255)

                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  2:2234370/1‑93 (MQ=255)

                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  1:3067339/1‑93 (MQ=255)

                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  1:2862633/1‑93 (MQ=255)

                                                      attatCAGTTGGGGACAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                   <  1:4114700/93‑1 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTCTTCGTACCGCGATCGATATGGATaaaa                                  >  1:27016/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  1:1628722/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCCAGATCGATATGGATaaaca                                 >  1:4467912/1‑91 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                >  1:221513/1‑93 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                >  1:221512/1‑93 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                >  1:4405777/1‑93 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                <  2:1344586/93‑1 (MQ=255)

                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                               >  2:2465238/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                            >  2:2914201/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                            >  1:3255456/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACagcg                            >  2:2346967/1‑93 (MQ=255)

                                                               tGGGGCCAACCATCCATCGACTTGTGGGTTAGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTc                          <  2:3611869/93‑1 (MQ=255)

                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                      >  1:581741/1‑93 (MQ=255)

                                                                     cAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCt                    >  1:412036/1‑93 (MQ=255)

                                                                     cAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCt                    >  2:2834040/1‑93 (MQ=255)

                                                                     cAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCt                    >  2:1106796/1‑93 (MQ=255)

                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                  >  2:4666548/1‑93 (MQ=255)

                                                                        cAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAg                 >  2:450981/1‑93 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                >  1:3871089/1‑93 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                <  1:3333076/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                <  1:1570363/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                >  1:1497154/1‑93 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               >  2:1437117/1‑93 (MQ=255)

                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              <  1:3117777/93‑1 (MQ=255)

                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt             <  1:813201/93‑1 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           <  2:4631369/93‑1 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           <  2:42666/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGCCCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        <  2:2256317/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        >  1:365955/1‑93 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       >  2:3676949/1‑93 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  1:4455470/1‑93 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCCCAGCGGTTACGTCa      <  1:3345621/93‑1 (MQ=255)

                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt    <  2:4232519/93‑1 (MQ=255)

                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt    <  1:2486842/93‑1 (MQ=255)

                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAACACCGCGTCTTTGCTCAGCGGTTACGTCAGTc   >  1:3509479/1‑93 (MQ=255)

                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc   <  2:4511147/93‑1 (MQ=255)

                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc   <  1:1003558/93‑1 (MQ=255)

                                                                                       tgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCg  <  1:2418268/93‑1 (MQ=255)

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`