BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,071,190	T→C	T65A (ACG→GCG)	b1010 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,071,190	0	T	C	99.2%	382.6 / ‑4.6	121	T65A (ACG→GCG)	b1010	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (67/53); total (67/54)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 131 total aligned reads displayed.

CGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAG  >  NC_000913/1071100‑1071279

cGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGcct                                                                                          <  2:502781/93‑1 (MQ=255)

cGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGcct                                                                                          <  1:1294625/93‑1 (MQ=255)

 ggCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGcctc                                                                                         <  2:2004234/93‑1 (MQ=255)

       gATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCAc                                                                                   >  1:730781/1‑93 (MQ=255)

       gATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCAc                                                                                   >  1:730904/1‑93 (MQ=255)

        aTTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCAcc                                                                                  <  1:2711900/93‑1 (MQ=255)

         tttCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCt                                                                                 <  2:3056336/93‑1 (MQ=255)

         tttCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCt                                                                                 >  2:1546500/1‑93 (MQ=255)

           tCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTg                                                                               >  2:3848352/1‑93 (MQ=255)

            cGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGc                                                                              >  2:2772168/1‑93 (MQ=255)

              ttAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCgg                                                                            <  1:1782069/93‑1 (MQ=255)

                 aTCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATa                                                                         <  2:2327491/93‑1 (MQ=255)

                 aTCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATa                                                                         >  2:1306602/1‑93 (MQ=255)

                  tCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAg                                                                        >  1:387753/1‑93 (MQ=255)

                    gcggcgTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGtt                                                                      <  2:3742800/93‑1 (MQ=255)

                      ggcgTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTc                                                                    <  1:2748531/93‑1 (MQ=255)

                       gcgTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTcc                                                                   <  1:4638808/93‑1 (MQ=255)

                        cgTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCa                                                                  <  1:3452975/93‑1 (MQ=255)

                         gTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAg                                                                 >  1:809768/1‑93 (MQ=255)

                         gTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAg                                                                 >  2:60462/1‑93 (MQ=255)

                         gTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAg                                                                 >  1:1183174/1‑93 (MQ=255)

                         gTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGACAGTTTCCAg                                                                 >  2:296874/1‑93 (MQ=255)

                           aaTTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCCCCTTGCGGATAGTTTCCAGaa                                                               <  2:1045563/93‑1 (MQ=255)

                           aaTTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGaa                                                               <  2:684909/93‑1 (MQ=255)

                            aTTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAAc                                                              <  1:3265963/93‑1 (MQ=255)

                            aTTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAAc                                                              >  2:2339970/1‑93 (MQ=255)

                               tttCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTg                                                           <  2:1570039/93‑1 (MQ=255)

                               tttCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTg                                                           <  2:1777399/93‑1 (MQ=255)

                                  ccAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑g                                                       <  2:2311128/93‑1 (MQ=255)

                                  ccAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑g                                                       >  2:3787526/1‑93 (MQ=255)

                                   cAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑gg                                                      >  1:1609830/1‑93 (MQ=255)

                                     gTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGt                                                    <  2:3943971/93‑1 (MQ=255)

                                     gTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGt                                                    <  2:3078760/93‑1 (MQ=255)

                                      tCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGtt                                                   <  2:755186/93‑1 (MQ=255)

                                      tCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGtt                                                   <  2:2004397/93‑1 (MQ=255)

                                      tCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGtt                                                   <  2:2666806/93‑1 (MQ=255)

                                         gTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTgc                                                >  1:200489/1‑93 (MQ=255)

                                          taataaAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTgcg                                               >  1:3737484/1‑93 (MQ=255)

                                            ataaAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGcccc                                             <  1:1461799/93‑1 (MQ=255)

                                             taaAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCt                                            >  1:779056/1‑93 (MQ=255)

                                              aaaGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGccct                                           >  1:1714996/1‑91 (MQ=255)

                                              aaaCATCCTGTTGACGGTCACATCCGCCATCGTGCCCCCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCAtt                                           <  2:2707950/93‑1 (MQ=255)

                                               aaGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCttt                                          <  1:971069/93‑1 (MQ=255)

                                               aaGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCctt                                          >  2:3423690/1‑93 (MQ=255)

                                               aaGATGCTGTTGAAGGTCACATCCGCCATCGTCCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCttt                                          <  1:1796453/93‑1 (MQ=255)

                                                 gATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTgg                                        <  1:2609704/93‑1 (MQ=255)

                                                 gATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCttttg                                        >  2:3379912/1‑91 (MQ=255)

                                                  aTGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTggg                                       >  1:749087/1‑93 (MQ=255)

                                                   tGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGGTTTTCCCTTTTggg                                       >  2:3653499/1‑93 (MQ=255)

                                                   tGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGGTGCGCCTTTgggg                                      >  2:432393/1‑92 (MQ=255)

                                                    gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTc                                     <  1:1824739/93‑1 (MQ=255)

                                                     cTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCa                                    <  1:1340282/93‑1 (MQ=255)

                                                     cTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCa                                    <  1:320249/93‑1 (MQ=255)

                                                      tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCAt                                   <  2:3676312/93‑1 (MQ=255)

                                                      tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCAt                                   <  1:3019631/93‑1 (MQ=255)

                                                      tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTggggcct                                   >  2:1908891/1‑89 (MQ=255)

                                                       tttGAATGTCCCCTCCGCCCTCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  <  2:1394538/92‑1 (MQ=255)

                                                       tttGAAGCTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  <  2:4289146/92‑1 (MQ=255)

                                                       gTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  <  2:3594986/93‑1 (MQ=255)

                                                       gTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  <  2:3594985/93‑1 (MQ=255)

                                                       gTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  <  2:1013735/93‑1 (MQ=255)

                                                       gTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATc                                  >  1:1262550/1‑93 (MQ=255)

                                                       gTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGGCTTTGGGTGATc                                  >  1:109710/1‑93 (MQ=255)

                                                        ttGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCg                                 >  1:4551359/1‑93 (MQ=255)

                                                        ttGAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGGCATCg                                 >  1:4551358/1‑93 (MQ=255)

                                                          gAAGGTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCCTCGGc                               >  1:778299/1‑93 (MQ=255)

                                                             ggTCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCaaa                            >  1:512709/1‑93 (MQ=255)

                                                               tCACATCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACa                          <  1:1482083/93‑1 (MQ=255)

                                                                 acaTCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTGGGTCATCGGCAAACAGc                        >  2:4436155/1‑93 (MQ=255)

                                                                  caTCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCa                       <  1:3341521/93‑1 (MQ=255)

                                                                  caTCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGGCATCGGCAAACacaa                       >  1:1008765/1‑90 (MQ=255)

                                                                   aTCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTTGGTCATCGGCAAACAGCAc                      >  1:2138734/1‑93 (MQ=255)

                                                                   aTCCGCCATCGTGCCACCCGCCGCCTCGAACACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCAc                      >  2:2908082/1‑93 (MQ=255)

                                                                    tCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACg                     >  1:622268/1‑93 (MQ=255)

                                                                    tCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACg                     <  2:3055973/93‑1 (MQ=255)

                                                                    tCCGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTTGGGCATCGGCAAACAGCACg                     >  2:4342352/1‑93 (MQ=255)

                                                                     ccGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGt                    <  1:1551920/93‑1 (MQ=255)

                                                                      cGCCATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGtt                   <  2:2942474/93‑1 (MQ=255)

                                                                         cATCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTGGGTCATCGGCAAACAGCACGttatt                >  2:1183503/1‑93 (MQ=255)

                                                                          agcGTCCCACCCGCCGCCTAGCTCACCTTGCGGATAGTTTCCAGAAGGTGGC‑GGGTGTGCGCCTTTGGGTCATCGGCAAACAGCACGttatta               <  1:1598981/91‑1 (MQ=255)

                                                                          aTCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGttatta               <  2:3566190/93‑1 (MQ=255)

                                                                          aTCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTTCGCCTTTGGGTCCTCGGCAAACAGCACGgtatta               >  2:1794410/1‑93 (MQ=255)

                                                                           tCGTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTtattat              >  2:3312810/1‑93 (MQ=255)

                                                                             gTGCCCCCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGt            <  2:4381664/93‑1 (MQ=255)

                                                                             gTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCACCTTTGGGTCATCGGCAAACAGCACGTTATTATGt            <  2:1196909/93‑1 (MQ=255)

                                                                             gTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTTGGTCATCGGCAAACAGCACGTTATTATGt            >  2:2520854/1‑93 (MQ=255)

                                                                             gTGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGCTTGCGCCTTTGGGTCATCGGCAAACCGCACCTTATTATGt            >  1:2202438/1‑93 (MQ=255)

                                                                              tGCCACCTGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGtt           >  1:2260719/1‑93 (MQ=255)

                                                                              tGCCACCCGCCGCCTCGATCACCTTGCGTATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGtt           <  1:64367/93‑1 (MQ=255)

                                                                              tGCCACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTGGGGCATCGGCAAACAGCACGGTATTATGtt           >  2:38814/1‑93 (MQ=255)

                                                                                ccACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGa         >  1:2057276/1‑93 (MQ=255)

                                                                                ccACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGa         <  1:3035877/93‑1 (MQ=255)

                                                                                ccACCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGa         <  2:1346008/93‑1 (MQ=255)

                                                                                  ccccGCCGCCTCGATCACCTTGCGTATAGTTTACAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATc       <  1:1266614/92‑1 (MQ=255)

                                                                                  aCCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTGGGTCATGCGCAAACAGCACGGTATTATGTTGATc       >  2:1637001/1‑93 (MQ=255)

                                                                                  aCCCGCCGCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCCTTTGGTACTCGGCAAACAGCACGTTCTTATGTTGATc       >  1:1158484/1‑93 (MQ=255)

                                                                                     cgccgccgcGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCGTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCaaa    <  1:753845/93‑1 (MQ=255)

                                                                                      gccgccTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCaaaa   <  1:245958/93‑1 (MQ=255)

                                                                                       ccgCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGGTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAg  >  1:2914386/1‑93 (MQ=255)

                                                                                       ccgCCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGCTTGCGGCCTTGGGGGATTGGCAAACAGCCCGTTATTATGTTGAATaaaaa  >  1:4147877/1‑92 (MQ=255)

CGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGC‑GGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAG  >  NC_000913/1071100‑1071279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`