BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,802	G→A	E57K (GAG→AAG)	yabB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,802	0	G	A	97.2%	616.4 / 1.2	178	E57K (GAG→AAG)	yabB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (95/78); total (98/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 180 total aligned reads displayed.

CTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATG  >  NC_000913/89712‑89891

ctTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAg                                                                                         <  1:1583025/93‑1 (MQ=255)

 ttGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAga                                                                                        >  2:4383145/1‑92 (MQ=255)

 ttGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGc                                                                                        <  2:619645/93‑1 (MQ=255)

 ttGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGc                                                                                        >  1:2032978/1‑93 (MQ=255)

  tGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCa                                                                                       <  1:4239075/93‑1 (MQ=255)

  tGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGCAAATTATCAAGCa                                                                                       >  1:799579/1‑93 (MQ=255)

   gagaACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCaa                                                                                      >  2:3360360/1‑93 (MQ=255)

       aCGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAAt                                                                                  >  2:4007006/1‑93 (MQ=255)

         acTGCCAGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTa                                                                                <  1:4199714/92‑1 (MQ=255)

          cTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAt                                                                               <  2:4661203/93‑1 (MQ=255)

           ttccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              <  1:3477057/91‑2 (MQ=255)

           tGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              >  2:1780294/1‑92 (MQ=255)

           tGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              <  1:979402/93‑2 (MQ=255)

           tGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              <  1:1748028/93‑2 (MQ=255)

           tGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              <  2:4504687/93‑2 (MQ=255)

           tGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAtt                                                                              >  2:549526/1‑92 (MQ=255)

             ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                            >  1:3873576/1‑93 (MQ=255)

             ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                            >  1:4182474/1‑93 (MQ=255)

             ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                            >  2:2465026/1‑93 (MQ=255)

               ggTCAAATGGTTTGCACCATTTACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                          >  2:3281141/1‑93 (MQ=255)

               ggTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                          >  2:361313/1‑93 (MQ=255)

               ggTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                          <  1:4599305/93‑1 (MQ=255)

                 tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                        <  1:2108564/93‑1 (MQ=255)

                 tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                        >  1:1488889/1‑93 (MQ=255)

                 tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                        >  1:1383914/1‑93 (MQ=255)

                   aaaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGt                                                                      >  2:2287434/1‑93 (MQ=255)

                   aaaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGt                                                                      >  2:3751769/1‑93 (MQ=255)

                   aaaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGt                                                                      <  1:943273/93‑1 (MQ=255)

                   aaaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGt                                                                      >  1:1077621/1‑93 (MQ=255)

                    aaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTTCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTc                                                                     >  1:1066940/1‑93 (MQ=255)

                    aaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTc                                                                     <  2:2248999/93‑1 (MQ=255)

                     aTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCg                                                                    <  1:2901897/93‑1 (MQ=255)

                      tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                   <  2:1304398/93‑1 (MQ=255)

                      tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                   >  1:3017054/1‑93 (MQ=255)

                      tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                   <  1:3043971/93‑1 (MQ=255)

                         tttGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCa                                                                >  1:4374384/1‑93 (MQ=255)

                         tttGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCa                                                                <  1:4071860/93‑1 (MQ=255)

                          ttGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCAt                                                               <  2:4194475/93‑1 (MQ=255)

                               ccATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAcc                                                          >  1:2575963/1‑93 (MQ=255)

                                cATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAccc                                                         <  2:3659985/93‑1 (MQ=255)

                                cATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAccc                                                         <  2:605971/93‑1 (MQ=255)

                                  ttGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCTTCTGTCGAGCATGAACCCgg                                                       <  1:849319/93‑1 (MQ=255)

                                  ttGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCgg                                                       >  2:1892883/1‑93 (MQ=255)

                                   tGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGt                                                      <  2:2315741/93‑1 (MQ=255)

                                     aCATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTg                                                    <  2:272096/93‑1 (MQ=255)

                                      cATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGa                                                   <  1:241444/93‑1 (MQ=255)

                                      cATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGa                                                   <  2:1568496/93‑1 (MQ=255)

                                       aTTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAg                                                  >  1:2625024/1‑93 (MQ=255)

                                       aTTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAg                                                  >  2:3789435/1‑93 (MQ=255)

                                           aTCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGcc                                              >  2:239283/1‑93 (MQ=255)

                                           aTCACCCGTGCCTGCTGCTTTACACCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATCAACCCGGTTGAGCGcc                                              >  1:2839724/1‑93 (MQ=255)

                                            tCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCg                                             >  2:2756710/1‑93 (MQ=255)

                                              ccccGTTCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtg                                           <  2:537214/92‑1 (MQ=255)

                                                 cGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGc                                        <  2:648734/93‑1 (MQ=255)

                                                  gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCa                                       >  2:4325572/1‑93 (MQ=255)

                                                  gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCa                                       >  2:3928790/1‑93 (MQ=255)

                                                    gCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                                     >  1:4012872/1‑93 (MQ=255)

                                                     ccTGCTGCTTTACCCCCTGCCTGCATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGATGAGCGCCGTGCGCAgcg                                    >  2:146712/1‑93 (MQ=255)

                                                       tgctgcTTTCCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                  <  1:1857497/93‑1 (MQ=255)

                                                       tgctgcTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                  >  2:1037939/1‑93 (MQ=255)

                                                       tgctgcTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                  >  2:1130540/1‑93 (MQ=255)

                                                        gctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCt                                 <  2:2844582/93‑1 (MQ=255)

                                                          tgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTAc                               <  1:3134465/93‑1 (MQ=255)

                                                          tgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTAc                               <  1:9300/93‑1 (MQ=255)

                                                             tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                            <  1:1217508/93‑1 (MQ=255)

                                                             tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTAGTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                            <  2:2107311/93‑1 (MQ=255)

                                                              ttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGtt                           <  2:703575/93‑1 (MQ=255)

                                                              ttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGtt                           <  2:2203186/93‑1 (MQ=255)

                                                                aCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg                         <  2:1927550/93‑1 (MQ=255)

                                                                aCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg                         <  1:484977/93‑1 (MQ=255)

                                                                 cccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAgg                        <  1:961799/93‑1 (MQ=255)

                                                                 cccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAgg                        <  1:3521907/93‑1 (MQ=255)

                                                                  ccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGt                       >  2:3806358/1‑93 (MQ=255)

                                                                   cccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTc                      <  1:3670234/93‑1 (MQ=255)

                                                                   cccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTc                      >  2:2634639/1‑93 (MQ=255)

                                                                    cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                     <  2:3774421/93‑1 (MQ=255)

                                                                    cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                     <  2:824874/93‑1 (MQ=255)

                                                                    cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                     >  1:3866399/1‑93 (MQ=255)

                                                                       gcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc                  >  1:785489/1‑93 (MQ=255)

                                                                          tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg               >  2:2555368/1‑93 (MQ=255)

                                                                           gAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGGGCAGCGCCTACTGTTAGGTAATTCCAGc              >  2:1327259/1‑93 (MQ=255)

                                                                           gAAGGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGc              <  2:2774810/93‑1 (MQ=255)

                                                                             aTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGCCATGCCAGCGa            >  1:745860/1‑93 (MQ=255)

                                                                              tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa           >  1:1714613/1‑93 (MQ=255)

                                                                              tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa           <  1:3284671/93‑1 (MQ=255)

                                                                               gggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAAt          <  1:609220/93‑1 (MQ=255)

                                                                               gggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAAt          >  1:1410982/1‑93 (MQ=255)

                                                                                 gAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGt        >  2:282466/1‑93 (MQ=255)

                                                                                 gAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGt        >  1:895012/1‑93 (MQ=255)

                                                                                 gAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGAATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGt        >  2:2146492/1‑93 (MQ=255)

                                                                                   aaTTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCa      >  2:4446341/1‑93 (MQ=255)

                                                                                   aaTTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCa      >  1:2657091/1‑93 (MQ=255)

                                                                                     ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga    >  1:1634858/1‑93 (MQ=255)

                                                                                     ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga    <  2:2807673/93‑1 (MQ=255)

                                                                                     ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga    >  1:2327854/1‑93 (MQ=255)

                                                                                     ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga    <  2:2358013/93‑1 (MQ=255)

                                                                                      tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat   <  1:3374001/93‑1 (MQ=255)

                                                                                      tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat   >  2:4237548/1‑93 (MQ=255)

                                                                                       aTCAAGCAAAAATTATTGCGTCTGTCGAGCCTGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg  >  2:4272890/1‑93 (MQ=255)

                                                                                       aTCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg  >  2:3830946/1‑93 (MQ=255)

CTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATG  >  NC_000913/89712‑89891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`