BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,107,299	A→G	V289A (GTG→GCG)	ymdD ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,107,299	0	A	G	99.4%	516.6 / ‑5.4	161	V289A (GTG→GCG)	ymdD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (92/68); total (93/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 167 total aligned reads displayed.

GAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGT  >  NC_000913/1107210‑1107388

gAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCAt                                                                                        >  1:2739956/1‑93 (MQ=255)

 aaCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCAtt                                                                                       <  1:4733353/93‑1 (MQ=255)

 aaCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCAtt                                                                                       <  1:1925121/93‑1 (MQ=255)

  aCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTc                                                                                      >  2:633353/1‑93 (MQ=255)

  aCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTc                                                                                      >  1:204633/1‑93 (MQ=255)

  aCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTc                                                                                      >  2:4080080/1‑93 (MQ=255)

   ccAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCa                                                                                     <  2:2956813/93‑1 (MQ=255)

   ccAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCa                                                                                     >  1:2137366/1‑93 (MQ=255)

    cAGATAGATACACAGCGATGCGTTAACAAAATAAGTCGCCCGCGCTGACTGGAAGTTAAGCAACCGGTGGCCAAAGGAGAAGACCGCATTCAc                                                                                    <  1:2586145/93‑1 (MQ=255)

     agatagatAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGTAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCAcc                                                                                   <  2:1375513/93‑1 (MQ=255)

     agatagatAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCAcc                                                                                   >  1:370480/1‑93 (MQ=255)

      gatagataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCa                                                                                  >  1:3166286/1‑93 (MQ=255)

       atagataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCAt                                                                                 >  2:596390/1‑93 (MQ=255)

         agataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATcc                                                                               >  1:350720/1‑93 (MQ=255)

         agataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATcc                                                                               >  1:1507584/1‑93 (MQ=255)

         agataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATcc                                                                               <  1:448615/93‑1 (MQ=255)

         agataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATcc                                                                               >  2:901320/1‑93 (MQ=255)

          gataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGGCCGCATTCACCATCca                                                                              >  2:3399521/1‑93 (MQ=255)

          gataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCca                                                                              >  1:3350900/1‑93 (MQ=255)

           ataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCcac                                                                             >  2:394334/1‑93 (MQ=255)

            taAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCcaca                                                                            >  2:3358822/1‑93 (MQ=255)

            taAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCcaca                                                                            >  1:4612601/1‑93 (MQ=255)

            taAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCcaca                                                                            <  2:4638610/93‑1 (MQ=255)

                 aGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCg                                                                       >  2:3553319/1‑93 (MQ=255)

                 aGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCg                                                                       >  2:3553320/1‑93 (MQ=255)

                  gCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGa                                                                      <  1:4311200/93‑1 (MQ=255)

                    gATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAgg                                                                    >  2:1621990/1‑93 (MQ=255)

                    gATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAgg                                                                    >  2:945232/1‑93 (MQ=255)

                     aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGa                                                                   >  2:2475774/1‑93 (MQ=255)

                     aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGa                                                                   >  1:1158152/1‑93 (MQ=255)

                     aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGa                                                                   >  2:3721144/1‑93 (MQ=255)

                     aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGa                                                                   >  1:1820095/1‑93 (MQ=255)

                      tGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGAc                                                                  <  1:2453161/93‑1 (MQ=255)

                      tGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGAc                                                                  >  2:2841331/1‑93 (MQ=255)

                        cGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCa                                                                <  1:2784466/93‑1 (MQ=255)

                          ttAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATg                                                              <  1:61402/93‑1 (MQ=255)

                               aaaaTAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGAt                                                         >  2:3073873/1‑93 (MQ=255)

                                aaaTAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATc                                                        >  1:2204000/1‑93 (MQ=255)

                                aaaTAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAGGACCATGGTGAta                                                        >  2:3296294/1‑92 (MQ=255)

                                  aTAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACAGCATTCACCATCCACAGACCGAGGACCATGGTGATCAc                                                      >  1:3895264/1‑93 (MQ=255)

                                   tAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCACCCACAGACCGAGGACCATGGTGATCAcc                                                     <  1:1562787/93‑1 (MQ=255)

                                     aGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGa                                                   >  2:4444355/1‑93 (MQ=255)

                                     aGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGa                                                   >  1:3857871/1‑93 (MQ=255)

                                      gTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGAc                                                  >  1:1794371/1‑93 (MQ=255)

                                        cACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGCGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCCCCGACTc                                                >  1:290339/1‑93 (MQ=255)

                                        cACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTc                                                <  1:4092293/93‑1 (MQ=255)

                                        cACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTc                                                <  2:3297523/93‑1 (MQ=255)

                                        cACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTc                                                <  2:3297525/93‑1 (MQ=255)

                                         ccccGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCCCCCACAGACCGAGGACCATGGTGATCACCGACTCg                                               <  2:1006850/92‑1 (MQ=255)

                                         aCCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCg                                               >  2:2554407/1‑93 (MQ=255)

                                          cccGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCgg                                              >  2:3638335/1‑93 (MQ=255)

                                             gcgcTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGttt                                           >  2:1298682/1‑93 (MQ=255)

                                              cgcTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTc                                          <  1:4745090/93‑1 (MQ=255)

                                                   aCTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACa                                     <  1:572770/93‑1 (MQ=255)

                                                     tGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATc                                   >  1:3668196/1‑93 (MQ=255)

                                                     tGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATc                                   <  1:2369358/93‑1 (MQ=255)

                                                      ggAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATcc                                  >  2:4399240/1‑93 (MQ=255)

                                                      ggAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATcc                                  >  2:1915011/1‑93 (MQ=255)

                                                      ggAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATcc                                  >  2:3506489/1‑93 (MQ=255)

                                                       gAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCa                                 <  1:2198034/93‑1 (MQ=255)

                                                       gAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCa                                 >  2:1438254/1‑93 (MQ=255)

                                                        aaGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAg                                <  2:1730910/93‑1 (MQ=255)

                                                        aaGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAg                                <  2:1020289/93‑1 (MQ=255)

                                                         aGTTAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAgg                               <  1:3077388/93‑1 (MQ=255)

                                                           ttAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCa                             >  2:1578781/1‑93 (MQ=255)

                                                            tAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCAt                            >  1:878230/1‑93 (MQ=255)

                                                            tAAGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCAt                            >  1:753380/1‑93 (MQ=255)

                                                             aaGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATc                           >  1:2553433/1‑93 (MQ=255)

                                                             aaGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATc                           <  2:1230474/93‑1 (MQ=255)

                                                             aaGCAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATc                           >  1:2553432/1‑93 (MQ=255)

                                                                cAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGcc                        <  2:1060289/93‑1 (MQ=255)

                                                                cAAACGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGcc                        >  1:1361409/1‑93 (MQ=255)

                                                                 aaaCGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCa                       >  1:2091633/1‑93 (MQ=255)

                                                                  aaCGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCAc                      <  2:3576821/93‑1 (MQ=255)

                                                                  aaCGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCAc                      >  2:1093287/1‑93 (MQ=255)

                                                                    cGGTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTg                    >  1:3622241/1‑93 (MQ=255)

                                                                     ggTGGCCAAAGGAGAAGACCGCATTCCCCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGc                   <  1:2532471/93‑1 (MQ=255)

                                                                     ggTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGc                   >  1:3578702/1‑93 (MQ=255)

                                                                     ggTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGc                   <  2:634634/93‑1 (MQ=255)

                                                                      gTGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGcc                  >  2:3870027/1‑93 (MQ=255)

                                                                       tGGCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCa                 >  1:4224103/1‑93 (MQ=255)

                                                                        ggCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCAt                <  1:4568096/93‑1 (MQ=255)

                                                                        ggCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCAt                >  2:4327557/1‑93 (MQ=255)

                                                                         gCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGCCTCGGTTTCGTACATCCAGGCATCGCCACTGCCATa               <  1:602962/93‑1 (MQ=255)

                                                                         gCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATa               <  1:3766785/93‑1 (MQ=255)

                                                                         gCCAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATa               >  1:3224825/1‑93 (MQ=255)

                                                                           cAAAGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAgc             >  2:3625973/1‑93 (MQ=255)

                                                                             aaGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAgcgc           <  2:1185030/93‑1 (MQ=255)

                                                                             aaGGAGAAAGCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAgcgc           <  1:1153387/93‑1 (MQ=255)

                                                                              aGGAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCt          >  2:2350299/1‑93 (MQ=255)

                                                                               ggAGAAGACCGCATTCCCCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTg         <  1:2417851/93‑1 (MQ=255)

                                                                               ggAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTg         >  2:3163478/1‑93 (MQ=255)

                                                                               ggAGAAGACCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTg         >  1:3549525/1‑93 (MQ=255)

                                                                                 agaagaCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGt       >  1:1498051/1‑93 (MQ=255)

                                                                                   aagaCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTg     >  1:4188969/1‑93 (MQ=255)

                                                                                   aagaCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTg     >  1:3055279/1‑93 (MQ=255)

                                                                                     gaCCGCATTCCCCATCCACAGACCGAGGTCCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAg   >  2:4614358/1‑93 (MQ=255)

                                                                                      aCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAga  >  2:1678033/1‑92 (MQ=255)

                                                                                      aCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGt  >  2:2696342/1‑93 (MQ=255)

                                                                                      aCCGCATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGt  >  2:2696343/1‑93 (MQ=255)

GAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGT  >  NC_000913/1107210‑1107388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`