BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,435	A→G	S52S (AGT→AGC)	rne ←	RNase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,435	0	A	G	99.3%	481.6 / ‑4.6	149	S52S (AGT→AGC)	rne	RNase E
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (98/50); total (98/51)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 154 total aligned reads displayed.

GTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACG  >  NC_000913/1143345‑1143524

gTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCt                                                                                         >  1:1605532/1‑93 (MQ=255)

gTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCt                                                                                         >  2:2027651/1‑93 (MQ=255)

 tAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTc                                                                                        >  2:2713521/1‑93 (MQ=255)

 tAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTc                                                                                        <  1:1306673/93‑1 (MQ=255)

   gTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCgg                                                                                      >  1:384197/1‑93 (MQ=255)

    ttAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGt                                                                                     >  1:3623281/1‑93 (MQ=255)

     tAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGtt                                                                                    >  1:3668968/1‑93 (MQ=255)

      aGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATAAACAAAAGCAGCTTCCAGGCTCGGTTc                                                                                   >  1:2941655/1‑93 (MQ=255)

      aGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTAAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTc                                                                                   >  1:2784327/1‑93 (MQ=255)

       gCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCa                                                                                  <  2:1399891/93‑1 (MQ=255)

        cAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCaa                                                                                 >  2:3483673/1‑93 (MQ=255)

        cAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCaa                                                                                 <  2:1931790/93‑1 (MQ=255)

         aGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGT‑ATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATg                                                                               <  1:2950302/93‑1 (MQ=255)

           ggAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGc                                                                              >  1:3494794/1‑93 (MQ=255)

           ggAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGCGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATgg                                                                              >  1:3896387/1‑92 (MQ=255)

            gAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCg                                                                             >  2:1795421/1‑93 (MQ=255)

            gAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCg                                                                             <  1:535932/93‑1 (MQ=255)

             aaaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCgg                                                                            <  1:3735076/93‑1 (MQ=255)

             aaaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCgg                                                                            <  2:3820914/93‑1 (MQ=255)

              aaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCggg                                                                           >  2:4729428/1‑93 (MQ=255)

                tatTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTg                                                                         >  2:1553315/1‑93 (MQ=255)

                 atTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGGGa                                                                        >  2:169062/1‑93 (MQ=255)

                  ttCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAt                                                                       >  1:3537605/1‑93 (MQ=255)

                    cgcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGATGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAttt                                                                     <  2:2388230/93‑1 (MQ=255)

                    cgcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAttt                                                                     <  2:531464/93‑1 (MQ=255)

                     gcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTCATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAtttt                                                                    >  2:3592775/1‑93 (MQ=255)

                     gcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAtttt                                                                    >  1:3101684/1‑93 (MQ=255)

                     gcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAtttt                                                                    >  2:1233333/1‑93 (MQ=255)

                      cgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTa                                                                   <  1:4235875/93‑1 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  1:3651379/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  1:483774/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  1:2648110/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  2:2364893/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGGGATTTTAc                                                                  >  1:1529738/1‑93 (MQ=255)

                        ggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAcc                                                                 >  1:712994/1‑93 (MQ=255)

                        ggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAcc                                                                 >  1:4139630/1‑93 (MQ=255)

                          cAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCtt                                                               <  2:4466369/93‑1 (MQ=255)

                          cAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCtt                                                               <  1:1391277/93‑1 (MQ=255)

                           aaTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCttt                                                              <  1:2147782/93‑1 (MQ=255)

                           aaTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCttt                                                              >  2:2174853/1‑93 (MQ=255)

                            aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTg                                                             >  1:2420810/1‑93 (MQ=255)

                            aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTg                                                             >  1:4437479/1‑93 (MQ=255)

                            aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTg                                                             <  2:3196730/93‑1 (MQ=255)

                            aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTg                                                             <  1:3096010/93‑1 (MQ=255)

                                cTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGa                                                         <  1:1786218/93‑1 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        >  2:4357428/1‑93 (MQ=255)

                                    tAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGtt                                                     <  2:4185268/93‑1 (MQ=255)

                                     aGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGttt                                                    >  2:2807918/1‑93 (MQ=255)

                                         ggaggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATTCGGCTGATTCTACCTTTGTAGCTGTTTGCCt                                                >  2:4407206/1‑93 (MQ=255)

                                         ggaggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCt                                                >  1:556663/1‑93 (MQ=255)

                                           aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCttt                                              >  2:92914/1‑93 (MQ=255)

                                           aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCttt                                              <  1:3234986/93‑1 (MQ=255)

                                             gaAACCTGGACTTTCAGCGCCGTAACCAACAAAAGCACCTTCCAGCCCGGTTTCAATGGGGGTGTTTTTACTTTTGTAGATGTTTGCtttttt                                            <  2:3094791/93‑1 (MQ=255)

                                              aaaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTc                                           >  2:4077321/1‑93 (MQ=255)

                                               aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCt                                          <  2:215694/93‑1 (MQ=255)

                                               aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCt                                          >  2:461837/1‑93 (MQ=255)

                                               aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCt                                          <  1:3671506/93‑1 (MQ=255)

                                                aCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTg                                         >  1:2381797/1‑93 (MQ=255)

                                                 ccGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGc                                        <  1:1345521/93‑1 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTc                                      <  2:1560912/93‑1 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTc                                      <  1:272568/93‑1 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTc                                      >  2:724475/1‑93 (MQ=255)

                                                    tGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCg                                     >  1:3768111/1‑93 (MQ=255)

                                                    tGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGCGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCg                                     >  2:1665878/1‑93 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    <  1:2572877/93‑1 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    >  2:3069671/1‑93 (MQ=255)

                                                        gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGcc                                 <  1:4539713/93‑1 (MQ=255)

                                                        gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGcc                                 <  1:4539714/93‑1 (MQ=255)

                                                        gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGcc                                 >  2:731714/1‑93 (MQ=255)

                                                         ttCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCTGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGccc                                <  1:2722793/93‑1 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             <  2:340771/93‑1 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             <  2:340772/93‑1 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCGTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             >  1:941407/1‑93 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGGGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             >  1:93030/1‑93 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             <  1:4029693/93‑1 (MQ=255)

                                                                  gTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTc                       >  2:2443036/1‑93 (MQ=255)

                                                                  gTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTc                       >  1:1127369/1‑93 (MQ=255)

                                                                   tAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCg                      >  1:199714/1‑93 (MQ=255)

                                                                   tAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCg                      >  1:2787693/1‑93 (MQ=255)

                                                                   tAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCg                      <  1:4720581/93‑1 (MQ=255)

                                                                    aaTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGa                     >  1:1218571/1‑93 (MQ=255)

                                                                    aaTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGa                     >  2:3019696/1‑93 (MQ=255)

                                                                     aTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGat                    <  1:219833/93‑1 (MQ=255)

                                                                     aTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGat                    >  1:4236670/1‑93 (MQ=255)

                                                                      tCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGata                   <  1:4465113/93‑1 (MQ=255)

                                                                        aacaaAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATc                 >  2:3888697/1‑93 (MQ=255)

                                                                         acaaAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATcc                <  2:1164080/93‑1 (MQ=255)

                                                                           aaaaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAg              <  2:3366559/93‑1 (MQ=255)

                                                                            aaaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAgg             >  2:2537961/1‑93 (MQ=255)

                                                                             aaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGt            >  1:3957923/1‑93 (MQ=255)

                                                                              agcagcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTc           >  1:211269/1‑93 (MQ=255)

                                                                               gcagcTTCCAGGTTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCa          >  1:1432930/1‑93 (MQ=255)

                                                                                cagcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCAt         >  2:4032890/1‑93 (MQ=255)

                                                                                 agcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATa        >  2:1504375/1‑93 (MQ=255)

                                                                                 agcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATa        >  2:4447643/1‑93 (MQ=255)

                                                                                  gcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATAc       >  2:1222558/1‑93 (MQ=255)

                                                                                      ccAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGAc   >  2:1533643/1‑93 (MQ=255)

                                                                                      ccAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGAc   <  2:1157136/93‑1 (MQ=255)

                                                                                       cAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACg  >  2:1480620/1‑93 (MQ=255)

                                                                                       cAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACg  >  2:643199/1‑93 (MQ=255)

GTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACG  >  NC_000913/1143345‑1143524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`