BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,672	A→G	K170K (AAA→AAG)	yceC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,672	0	A	G	98.8%	516.8 / ‑2.4	161	K170K (AAA→AAG)	yceC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (91/68); total (92/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 174 total aligned reads displayed.

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC  >  NC_000913/1144582‑1144763

tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                           <  1:4411459/93‑1 (MQ=255)

tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                           <  1:676181/93‑1 (MQ=255)

 cATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAgggg                                                                                          <  2:1036754/93‑1 (MQ=255)

  aTCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGa                                                                                         >  1:1089267/1‑93 (MQ=255)

   tCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGag                                                                                        >  1:3497954/1‑92 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                      >  1:3853910/1‑93 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                      >  2:3581355/1‑93 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                      <  2:613628/93‑1 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAGAAGGGGATGCa                                                                                     >  1:3630036/1‑93 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                     >  1:2290253/1‑93 (MQ=255)

       cTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaa                                                                                    <  1:2117578/93‑1 (MQ=255)

          gACCGGGACGCCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaaaa                                                                                 >  1:1917864/1‑93 (MQ=255)

          gACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaaaa                                                                                 <  2:2358756/93‑1 (MQ=255)

          gACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaaaa                                                                                 <  1:4630834/93‑1 (MQ=255)

           aCCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAg                                                                                <  1:3955500/93‑1 (MQ=255)

            ccGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGa                                                                               <  1:4725257/93‑1 (MQ=255)

            ccGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGa                                                                               <  1:1514466/93‑1 (MQ=255)

              gggACCCCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                             <  1:3622481/93‑1 (MQ=255)

              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                             >  2:3877603/1‑93 (MQ=255)

              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                             >  2:2043485/1‑93 (MQ=255)

               ggtcacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTa                                                                            <  1:2012684/90‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                            <  1:2076335/93‑1 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                            >  1:2675010/1‑93 (MQ=255)

                gACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAc                                                                           <  1:1333872/93‑1 (MQ=255)

                gACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAc                                                                           <  2:1239393/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                         <  2:3270879/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                         >  2:3263095/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGATAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                      <  2:2578215/93‑1 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                      <  2:2927738/93‑1 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                      >  1:2383413/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                      >  1:1833776/1‑93 (MQ=255)

                      tCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcg                                                                     >  1:2724180/1‑93 (MQ=255)

                      tCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcg                                                                     >  1:891709/1‑93 (MQ=255)

                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                   >  1:3325083/1‑93 (MQ=255)

                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                   >  2:2383921/1‑93 (MQ=255)

                          gtgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTgg                                                                 >  1:1069883/1‑93 (MQ=255)

                           tgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGt                                                                <  1:1276172/93‑1 (MQ=255)

                           tgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGt                                                                >  1:2849903/1‑93 (MQ=255)

                           tgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGt                                                                >  1:2207000/1‑93 (MQ=255)

                            gtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTg                                                               >  1:544875/1‑93 (MQ=255)

                            gtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTg                                                               >  2:768231/1‑93 (MQ=255)

                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                              >  1:4287590/1‑93 (MQ=255)

                              tttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcg                                                             <  1:1205974/93‑1 (MQ=255)

                               ttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcgc                                                            <  2:4268459/93‑1 (MQ=255)

                                   tGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTc                                                        >  2:4125021/1‑93 (MQ=255)

                                   tGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTc                                                        <  1:454015/93‑1 (MQ=255)

                                       aGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACTTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTg                                                    <  1:1135140/93‑1 (MQ=255)

                                         cGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGc                                                  >  2:3500564/1‑93 (MQ=255)

                                          gAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCa                                                 >  1:2276914/1‑93 (MQ=255)

                                          gAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCa                                                 <  2:1322090/93‑1 (MQ=255)

                                           aaaaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAg                                                >  2:3329006/1‑93 (MQ=255)

                                            aaaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGt                                               >  1:704808/1‑93 (MQ=255)

                                            aaaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGt                                               >  2:30891/1‑93 (MQ=255)

                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGACAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGtag                                             >  2:4620912/1‑91 (MQ=255)

                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGACAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                            >  2:527893/1‑93 (MQ=255)

                                                 cGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAt                                          <  2:1915600/93‑1 (MQ=255)

                                                 cGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAt                                          <  1:1728972/93‑1 (MQ=255)

                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                         >  1:3032986/1‑93 (MQ=255)

                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                         >  2:1520234/1‑93 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                        <  2:4472195/93‑1 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                        <  2:1897669/93‑1 (MQ=255)

                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                       >  1:2464024/1‑93 (MQ=255)

                                                     cGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGa                                      <  1:2948349/93‑1 (MQ=255)

                                                      ggCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGCGCGCGGTCAGTGGCAGTCGCATGTGaa                                     >  1:4095335/1‑93 (MQ=255)

                                                         gttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTAGCATGCGAatag                                  >  1:2754852/1‑93 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                                 <  1:1739751/93‑1 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                                 >  1:4316992/1‑93 (MQ=255)

                                                            gcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                               >  2:2720271/1‑93 (MQ=255)

                                                            gcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                               <  1:3523283/93‑1 (MQ=255)

                                                            gcgtgCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                               <  2:4449291/93‑1 (MQ=255)

                                                             cgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGtt                              <  2:1622046/93‑1 (MQ=255)

                                                              gttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTc                             <  2:2111788/93‑1 (MQ=255)

                                                               ttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCa                            >  1:3252423/1‑93 (MQ=255)

                                                                 ctctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAg                          >  2:1988273/1‑93 (MQ=255)

                                                                 ctctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAg                          >  1:929959/1‑93 (MQ=255)

                                                                  tctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGc                         >  2:4339385/1‑93 (MQ=255)

                                                                     gCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAcc                      <  2:317107/93‑1 (MQ=255)

                                                                       ctGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    <  2:2210205/92‑1 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    >  1:726159/1‑93 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    <  2:2322246/93‑1 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    >  1:3068213/1‑93 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    >  1:1807075/1‑93 (MQ=255)

                                                                       aTGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGt                    >  1:1815475/1‑93 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                   >  2:3475137/1‑93 (MQ=255)

                                                                         gAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTa                  >  1:113005/1‑93 (MQ=255)

                                                                         gAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTa                  >  1:3706169/1‑93 (MQ=255)

                                                                           gCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACt                >  1:731695/1‑93 (MQ=255)

                                                                             aaTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGa              >  2:1723809/1‑93 (MQ=255)

                                                                             aaTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGa              >  1:3991623/1‑93 (MQ=255)

                                                                             aaTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGa              <  2:108277/93‑1 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa             <  1:2174323/93‑1 (MQ=255)

                                                                                  cGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAat         <  1:595901/93‑1 (MQ=255)

                                                                                  cGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAat         <  1:3995483/93‑1 (MQ=255)

                                                                                   gTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAata        >  2:4104517/1‑93 (MQ=255)

                                                                                    tGAAAAGGGGTTGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAatat       >  2:1237138/1‑93 (MQ=255)

                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt      >  2:435049/1‑93 (MQ=255)

                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt      >  1:4649324/1‑93 (MQ=255)

                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt      >  1:4335939/1‑93 (MQ=255)

                                                                                      aaaaaGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc     <  2:4330478/93‑1 (MQ=255)

                                                                                         aaGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGc  >  2:4012578/1‑93 (MQ=255)

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCTGC  >  NC_000913/1144582‑1144763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`