BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,731	T→C	T109A (ACC→GCC)	ycfQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,731	0	T	C	99.3%	466.5 / ‑4.7	144	T109A (ACC→GCC)	ycfQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (59/84); total (59/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 145 total aligned reads displayed.

ATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGG  >  NC_000913/1167641‑1167820

aTTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaa                                                                                         >  1:3091405/1‑93 (MQ=255)

aTTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaa                                                                                         <  1:474848/93‑1 (MQ=255)

 tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                        <  2:1466570/93‑1 (MQ=255)

 tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                        <  1:2079345/93‑1 (MQ=255)

 tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCaaa                                                                                        <  1:1515500/93‑1 (MQ=255)

  ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAg                                                                                       >  2:2672991/1‑93 (MQ=255)

  ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAg                                                                                       <  2:694002/93‑1 (MQ=255)

   tCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAga                                                                                      >  1:320981/1‑92 (MQ=255)

   tCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGc                                                                                      <  1:1232485/93‑1 (MQ=255)

     aaCGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAg                                                                                    <  2:3688202/93‑1 (MQ=255)

      aCGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGt                                                                                   <  1:35736/93‑1 (MQ=255)

       cGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGtt                                                                                  <  2:1334017/93‑1 (MQ=255)

       cGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGtt                                                                                  <  1:3273873/93‑1 (MQ=255)

        gTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTg                                                                                 <  1:52131/93‑1 (MQ=255)

        gTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTg                                                                                 <  1:3029522/93‑1 (MQ=255)

        gTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGCGGTGTCTTTGCTGGCAAAGCAGTTg                                                                                 >  1:69861/1‑93 (MQ=255)

            tGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGa                                                                             <  2:4542096/93‑1 (MQ=255)

             ggCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGAt                                                                            <  1:2203430/93‑1 (MQ=255)

              gCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGAGGTGTCTTTGCTGGCAAAGCAGGTGGCGATg                                                                           >  2:1837723/1‑93 (MQ=255)

              gCGATATCTCCGGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATg                                                                           <  2:2714845/93‑1 (MQ=255)

                gATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgc                                                                         >  2:2061701/1‑93 (MQ=255)

                gATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgc                                                                         <  2:4400406/93‑1 (MQ=255)

                gATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgc                                                                         <  2:930518/93‑1 (MQ=255)

                  tatCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcag                                                                       >  1:1015454/1‑93 (MQ=255)

                   atCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGgcagc                                                                      >  2:2662850/1‑93 (MQ=255)

                     ctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaa                                                                    >  2:1621852/1‑93 (MQ=255)

                       ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                  <  1:275323/93‑1 (MQ=255)

                       ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                  >  1:4633298/1‑93 (MQ=255)

                       ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                  >  1:1835110/1‑93 (MQ=255)

                       ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCaaaa                                                                  <  2:1973204/93‑1 (MQ=255)

                        cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAAt                                                                 >  1:4280372/1‑93 (MQ=255)

                        cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAAt                                                                 >  1:4005507/1‑93 (MQ=255)

                        cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAAt                                                                 >  2:1295221/1‑93 (MQ=255)

                           aaTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCATTTGGCGATGGCAGCAAAATAGt                                                              <  1:3244641/93‑1 (MQ=255)

                           aaTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGt                                                              >  2:3375801/1‑93 (MQ=255)

                             tCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTca                                                            <  2:4375975/93‑1 (MQ=255)

                             tCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTca                                                            <  2:3797077/93‑1 (MQ=255)

                              cTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcag                                                           <  1:550958/93‑1 (MQ=255)

                               tGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagc                                                          >  2:3139772/1‑93 (MQ=255)

                                ggggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcc                                                         >  2:4489805/1‑93 (MQ=255)

                                ggggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcc                                                         <  1:1145290/93‑1 (MQ=255)

                                ggggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcc                                                         <  2:4211417/93‑1 (MQ=255)

                                 gggAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagcca                                                        >  2:1868941/1‑93 (MQ=255)

                                   gagaGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTcagccagc                                                      >  1:1490536/1‑93 (MQ=255)

                                    agagGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAgcg                                                     <  1:2441585/93‑1 (MQ=255)

                                     gagGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAgcgc                                                    >  1:3918116/1‑93 (MQ=255)

                                      agGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCa                                                   <  1:4314394/93‑1 (MQ=255)

                                       gggTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAg                                                  >  2:2685444/1‑93 (MQ=255)

                                        ggtggtGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGa                                                 <  2:3586647/93‑1 (MQ=255)

                                          tggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACt                                               <  2:4110297/93‑1 (MQ=255)

                                           ggtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTc                                              <  2:3019809/93‑1 (MQ=255)

                                            gtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTcc                                             <  1:1579085/93‑1 (MQ=255)

                                            gtgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTcc                                             >  2:1514692/1‑93 (MQ=255)

                                             tgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCa                                            >  1:104208/1‑93 (MQ=255)

                                             tgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCa                                            <  1:2701120/93‑1 (MQ=255)

                                             tgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCa                                            <  2:227981/93‑1 (MQ=255)

                                             tgCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCa                                            <  2:3071124/93‑1 (MQ=255)

                                                  ttgttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCtt                                       <  1:2224757/93‑1 (MQ=255)

                                                   tgttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCttt                                      <  2:624660/93‑1 (MQ=255)

                                                   tgttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCttt                                      <  2:3912272/93‑1 (MQ=255)

                                                    gttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCtttt                                     <  1:827421/93‑1 (MQ=255)

                                                    gttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCtttt                                     <  1:827420/93‑1 (MQ=255)

                                                     ttgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTACACGCTTTtc                                    >  1:1016722/1‑93 (MQ=255)

                                                       gATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTtctc                                  >  1:3130435/1‑93 (MQ=255)

                                                       gATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTtctc                                  >  1:1395480/1‑93 (MQ=255)

                                                        aTCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTctct                                 >  2:4126894/1‑93 (MQ=255)

                                                         tCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCtt                                >  1:4247393/1‑93 (MQ=255)

                                                         tCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAACGCAGACTACACGCTTTTttctc                                >  2:3512993/1‑92 (MQ=255)

                                                          cATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTc                               >  1:2567286/1‑93 (MQ=255)

                                                           aTGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGCCTCCACGCTTTTCTCTTca                              >  2:2852918/1‑93 (MQ=255)

                                                           aTGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTca                              <  1:952636/93‑1 (MQ=255)

                                                           aTGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTca                              >  2:3133014/1‑93 (MQ=255)

                                                           aTGAAGCAGCCTACCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTca                              >  1:2634450/1‑93 (MQ=255)

                                                            tGAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTcac                             <  2:4376717/93‑1 (MQ=255)

                                                             gAAGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTcaca                            <  1:2869001/93‑1 (MQ=255)

                                                              aaGCAGCCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACaa                           >  2:937309/1‑93 (MQ=255)

                                                                gcagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACaaaa                         >  2:2307511/1‑93 (MQ=255)

                                                                gcagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACaaaa                         <  1:3859228/93‑1 (MQ=255)

                                                                 cagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAAc                        >  2:385089/1‑93 (MQ=255)

                                                                 cagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAAc                        <  2:359484/93‑1 (MQ=255)

                                                                 cagcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAAc                        >  1:3680477/1‑93 (MQ=255)

                                                                  agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                       >  2:1664586/1‑93 (MQ=255)

                                                                  agcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACa                       <  1:2427398/93‑1 (MQ=255)

                                                                   gcCTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAg                      <  1:3834111/93‑1 (MQ=255)

                                                                     cTGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCt                    <  2:1004284/93‑1 (MQ=255)

                                                                      tGCCGGGGTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCtg                   <  1:4462620/93‑1 (MQ=255)

                                                                          ggggTGTCTTTGCTGGCAAAGCAGTTGGCGATGTCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCt               <  1:33149/93‑1 (MQ=255)

                                                                          ggggTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCt               >  1:623753/1‑93 (MQ=255)

                                                                           gggTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCtt              <  1:2652701/93‑1 (MQ=255)

                                                                            ggTGTCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTc             <  1:3209315/93‑1 (MQ=255)

                                                                             gtgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCa            >  2:1621655/1‑93 (MQ=255)

                                                                             gtgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCa            <  2:457577/93‑1 (MQ=255)

                                                                             gtgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCa            <  1:1355024/93‑1 (MQ=255)

                                                                              tgtCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCAt           >  2:3958370/1‑93 (MQ=255)

                                                                                tCTTTGCTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGc         >  1:2801639/1‑93 (MQ=255)

                                                                                     gCTGGCAAAGCAGTTGGCGATTGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGc    <  1:3400701/93‑1 (MQ=255)

                                                                                      cTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCg   <  1:3385656/93‑1 (MQ=255)

                                                                                      cTGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGAATCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCg   <  1:2548734/93‑1 (MQ=255)

                                                                                       tGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCgg  >  2:1656060/1‑93 (MQ=255)

                                                                                       tGGCAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCgg  >  2:1656062/1‑93 (MQ=255)

ATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGG  >  NC_000913/1167641‑1167820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`