BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,226,344	C→T	A118T (GCC→ACC)	ycgK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,344	0	C	T	99.5%	636.2 / ‑5.4	185	A118T (GCC→ACC)	ycgK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (88/96); total (88/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 192 total aligned reads displayed.

TTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226255‑1226433

ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtc                                                                                        <  1:4126941/93‑1 (MQ=255)

  aaCGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtcgt                                                                                      <  2:1898129/93‑1 (MQ=255)

  aaCGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtcgt                                                                                      <  1:935459/93‑1 (MQ=255)

   aCGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGtt                                                                                     <  2:2031012/93‑1 (MQ=255)

     gTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTc                                                                                   <  2:656416/93‑1 (MQ=255)

     gTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTc                                                                                   <  2:1516409/93‑1 (MQ=255)

     gTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTc                                                                                   >  1:1646091/1‑93 (MQ=255)

     gTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCAACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTc                                                                                   <  1:3160637/93‑1 (MQ=255)

      tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCg                                                                                  >  2:2106081/1‑93 (MQ=255)

      tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCg                                                                                  <  2:4516185/93‑1 (MQ=255)

       ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 <  1:1897113/93‑1 (MQ=255)

         aTGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTATCGTTTCGGGt                                                                               <  2:4678082/93‑1 (MQ=255)

          tGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGtt                                                                              <  1:102504/93‑1 (MQ=255)

            aCGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTg                                                                            >  1:2220352/1‑93 (MQ=255)

             cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGc                                                                           <  2:4382043/93‑1 (MQ=255)

              ggggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCa                                                                          >  1:21815/1‑93 (MQ=255)

              ggggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCa                                                                          >  2:3009657/1‑93 (MQ=255)

                 gagaGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAaca                                                                       >  1:2934108/1‑93 (MQ=255)

                    agTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTc                                                                    <  1:2040510/93‑1 (MQ=255)

                     gTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCg                                                                   >  1:3467198/1‑93 (MQ=255)

                     gTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCg                                                                   >  1:123867/1‑93 (MQ=255)

                     gTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCg                                                                   >  2:4258006/1‑93 (MQ=255)

                      gccccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGc                                                                  <  2:4038483/92‑1 (MQ=255)

                       ccccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCa                                                                 >  2:746108/1‑93 (MQ=255)

                        cccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAg                                                                >  1:2355662/1‑93 (MQ=255)

                         ccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAATACTCGCAGc                                                               >  1:2990233/1‑93 (MQ=255)

                         ccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGc                                                               >  2:2724754/1‑93 (MQ=255)

                          cGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCt                                                              >  2:3279424/1‑93 (MQ=255)

                          cGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACCCGCAGCt                                                              <  1:3670082/93‑1 (MQ=255)

                           gTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTc                                                             >  1:2893487/1‑93 (MQ=255)

                           gTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTc                                                             >  1:4446290/1‑93 (MQ=255)

                            tCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCa                                                            >  2:326026/1‑93 (MQ=255)

                              ggTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCata                                                          <  1:2663198/93‑1 (MQ=255)

                               gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         <  2:3832749/93‑1 (MQ=255)

                               gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  1:1667375/1‑93 (MQ=255)

                                 tGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAttt                                                       >  2:177123/1‑93 (MQ=255)

                                 tGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAttt                                                       <  1:4309014/93‑1 (MQ=255)

                                    cATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGcc                                                    >  1:50591/1‑93 (MQ=255)

                                    cATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGcc                                                    >  1:3379468/1‑93 (MQ=255)

                                     aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  2:885428/1‑93 (MQ=255)

                                        tACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGaa                                                >  1:3603294/1‑93 (MQ=255)

                                        tACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGaa                                                >  1:2277616/1‑93 (MQ=255)

                                           ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  1:1090419/93‑1 (MQ=255)

                                            tAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTTGCAGCTCATATTTGCCAGAAGCAg                                            <  1:2168592/93‑1 (MQ=255)

                                            tAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                                            <  2:3208179/93‑1 (MQ=255)

                                             aaTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                                           <  1:2402757/93‑1 (MQ=255)

                                             aaTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                                           <  2:3936221/93‑1 (MQ=255)

                                             aaTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                                           <  2:487684/93‑1 (MQ=255)

                                               tttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGga                                         <  1:2809175/93‑1 (MQ=255)

                                                ttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgag                                        <  1:2572226/93‑1 (MQ=255)

                                                  gAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtg                                      >  2:3050246/1‑93 (MQ=255)

                                                   aaTATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtga                                     <  2:1470817/93‑1 (MQ=255)

                                                   aaTATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAACTCATATTTGCCAGAAGCAgggaggga                                     >  1:140500/1‑93 (MQ=255)

                                                    atatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgag                                    >  1:306705/1‑93 (MQ=255)

                                                    atatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgag                                    <  1:3882176/93‑1 (MQ=255)

                                                      atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                                  >  2:711797/1‑93 (MQ=255)

                                                       tCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAt                                 >  1:4655972/1‑93 (MQ=255)

                                                       tCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAt                                 >  2:2657770/1‑93 (MQ=255)

                                                         gACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTg                               >  2:3430301/1‑93 (MQ=255)

                                                          aCGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGt                              >  2:730149/1‑93 (MQ=255)

                                                          aCGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGt                              <  1:243008/93‑1 (MQ=255)

                                                            gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            >  1:639243/1‑93 (MQ=255)

                                                            gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            >  1:468710/1‑93 (MQ=255)

                                                            gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            >  1:3545895/1‑93 (MQ=255)

                                                            gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            >  2:4347053/1‑93 (MQ=255)

                                                             ttATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCg                           <  1:2244267/93‑1 (MQ=255)

                                                             ttATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCg                           <  2:133024/93‑1 (MQ=255)

                                                             ttATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCg                           <  1:4367858/93‑1 (MQ=255)

                                                              tataTTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGt                          >  2:4735879/1‑93 (MQ=255)

                                                              tataTTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGt                          >  1:587531/1‑93 (MQ=255)

                                                              tataTTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGt                          <  1:719572/93‑1 (MQ=255)

                                                                  ttttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACt                      >  1:4675311/1‑93 (MQ=255)

                                                                   tttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTa                     <  2:276538/93‑1 (MQ=255)

                                                                    ttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTAt                    <  1:1489234/93‑1 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   <  1:3408514/93‑1 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   <  1:3432233/93‑1 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   <  1:2931166/93‑1 (MQ=255)

                                                                      ttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCg                  <  2:1535553/93‑1 (MQ=255)

                                                                         gttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGGGt               >  2:1223675/1‑93 (MQ=255)

                                                                         gttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGt               <  1:364/93‑1 (MQ=255)

                                                                          ttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGtt              <  1:90286/93‑1 (MQ=255)

                                                                          ttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGtt              >  1:700247/1‑93 (MQ=255)

                                                                           tttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTc             >  2:4686835/1‑93 (MQ=255)

                                                                           tttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTc             <  1:3918368/93‑1 (MQ=255)

                                                                           tttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTc             <  1:2345896/93‑1 (MQ=255)

                                                                           tttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTc             <  2:3094884/93‑1 (MQ=255)

                                                                             tgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTg           <  2:2209509/93‑1 (MQ=255)

                                                                             tgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTg           <  2:250468/93‑1 (MQ=255)

                                                                               tttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTggg         >  1:3272832/1‑93 (MQ=255)

                                                                               tttttACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTggg         <  2:2337641/93‑1 (MQ=255)

                                                                                ttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgggg        <  1:104701/93‑1 (MQ=255)

                                                                                 tttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGa       >  2:2397374/1‑93 (MQ=255)

                                                                                  ttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGCTCTAGGGaa      >  2:3781172/1‑93 (MQ=255)

                                                                                    aCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAata    >  1:3309861/1‑93 (MQ=255)

                                                                                    aCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAata    <  2:325803/93‑1 (MQ=255)

                                                                                      ggggGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGGGAATTGTCCGTGACTATCGAGTTCTGGGGAatatg  >  2:1788060/1‑92 (MQ=255)

                                                                                      gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  <  2:471318/93‑1 (MQ=255)

                                                                                      gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  >  1:2139244/1‑93 (MQ=255)

                                                                                      gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  >  1:1619543/1‑93 (MQ=255)

                                                                                      gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  <  2:2816877/93‑1 (MQ=255)

TTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226255‑1226433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`