BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,143	T→C	Y52H (TAC→CAC)	umuD →	UmuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,143	0	T	C	99.4%	540.8 / ‑5.0	167	Y52H (TAC→CAC)	umuD	UmuD
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (83/83); total (83/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 181 total aligned reads displayed.

GTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGC  >  NC_000913/1230053‑1230232

gTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcac                                                                                         >  1:3942381/1‑93 (MQ=255)

 ttCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcact                                                                                        <  2:1630011/93‑1 (MQ=255)

  tCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcactt                                                                                       <  1:4037997/93‑1 (MQ=255)

  tCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcactt                                                                                       <  1:1776059/93‑1 (MQ=255)

  tCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcactt                                                                                       >  1:3777143/1‑93 (MQ=255)

     gtgtGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGt                                                                                    <  2:4035081/93‑1 (MQ=255)

     gtgtGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGt                                                                                    >  1:3015124/1‑93 (MQ=255)

     gtgtGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGt                                                                                    >  2:2897167/1‑93 (MQ=255)

      tgtgGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTc                                                                                   <  1:780335/93‑1 (MQ=255)

      tgtgGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTc                                                                                   <  2:249843/93‑1 (MQ=255)

        tgGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCaa                                                                                 <  2:3046291/93‑1 (MQ=255)

         ggCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCaaa                                                                                <  2:4736606/93‑1 (MQ=255)

         ggCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCaaa                                                                                <  1:3432322/93‑1 (MQ=255)

          gCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAg                                                                               >  2:649520/1‑93 (MQ=255)

           cTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGc                                                                              >  1:4169677/1‑93 (MQ=255)

            tttCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCa                                                                             >  2:1120175/1‑93 (MQ=255)

            tttCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCa                                                                             >  2:1073710/1‑93 (MQ=255)

             ttCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCaa                                                                            >  2:2619544/1‑93 (MQ=255)

               ccTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgt                                                                          <  1:2646043/93‑1 (MQ=255)

               ccTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgt                                                                          <  1:948666/93‑1 (MQ=255)

                cTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtg                                                                         >  2:4447243/1‑93 (MQ=255)

                 ttCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtgg                                                                        >  2:2185649/1‑93 (MQ=255)

                   cACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtggtg                                                                      >  2:3868394/1‑93 (MQ=255)

                    aCCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGAGACTCACTTCGTCAAAGCAAgggggcg                                                                     >  1:2039595/1‑90 (MQ=255)

                     ccGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAt                                                                    <  2:1726701/93‑1 (MQ=255)

                        gcagcaGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCt                                                                 <  1:3385280/93‑1 (MQ=255)

                         cagcagATTACGTTGTACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTa                                                                <  1:2739036/93‑1 (MQ=255)

                         cagcagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTa                                                                <  2:740055/93‑1 (MQ=255)

                           gcagATTACTTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGCCTCACTTCGTCAAATCAAGTGGTGATTCTAtg                                                              <  2:1583834/93‑1 (MQ=255)

                            cagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtga                                                             >  2:2150638/1‑93 (MQ=255)

                            cagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtga                                                             <  2:2400517/93‑1 (MQ=255)

                            cagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtga                                                             <  1:1146031/93‑1 (MQ=255)

                             agATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAATTGGTGATTCTAtgat                                                            >  2:3887160/1‑93 (MQ=255)

                             agATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                                            >  2:1534818/1‑93 (MQ=255)

                             agATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                                            <  1:4493927/93‑1 (MQ=255)

                             agATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                                            <  2:4124519/93‑1 (MQ=255)

                                ttACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                                                         >  2:1240430/1‑93 (MQ=255)

                                ttACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                                                         <  2:3627411/93‑1 (MQ=255)

                                  aCGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                                                       <  2:4293917/93‑1 (MQ=255)

                                  aCGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                                                       <  1:1830669/93‑1 (MQ=255)

                                   cGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtgg                                                      <  2:1119013/93‑1 (MQ=255)

                                         aCAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAt                                                >  2:1533696/1‑93 (MQ=255)

                                          cAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                                               >  2:3097003/1‑93 (MQ=255)

                                            gcgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAg                                             <  1:2945974/93‑1 (MQ=255)

                                            gcgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAg                                             >  1:2189957/1‑93 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            <  2:3140320/93‑1 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            >  2:2700475/1‑93 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            <  2:3140319/93‑1 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            <  1:3475669/93‑1 (MQ=255)

                                              gcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                                           <  1:421819/93‑1 (MQ=255)

                                              gcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                                           <  2:2969576/93‑1 (MQ=255)

                                               cATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCAATTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                                          <  2:3594214/93‑1 (MQ=255)

                                                aTCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                                         >  1:3413848/1‑93 (MQ=255)

                                                 tCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACg                                        >  2:887851/1‑93 (MQ=255)

                                                  cGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACgg                                       >  2:4639792/1‑93 (MQ=255)

                                                   gATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt                                      <  2:1916672/93‑1 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     >  2:1578569/1‑93 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     >  1:2165010/1‑93 (MQ=255)

                                                     tCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa                                    >  1:4196271/1‑93 (MQ=255)

                                                        gAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGCGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt                                 >  2:4730862/1‑93 (MQ=255)

                                                           tCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACt                              <  2:2007115/93‑1 (MQ=255)

                                                            cAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg                             <  1:3486553/93‑1 (MQ=255)

                                                            cAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg                             >  2:1374213/1‑93 (MQ=255)

                                                            cAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg                             <  1:1361657/93‑1 (MQ=255)

                                                            cAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg                             >  1:1798903/1‑93 (MQ=255)

                                                             aaCTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGa                            >  1:3614575/1‑93 (MQ=255)

                                                              aCTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAt                           >  2:3325772/1‑93 (MQ=255)

                                                              aCTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGGGATTTACTGAt                           >  1:1510330/1‑93 (MQ=255)

                                                               cTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAtt                          <  2:2090308/93‑1 (MQ=255)

                                                                tGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTg                         >  1:67097/1‑93 (MQ=255)

                                                                  ttGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTc                       <  1:368548/93‑1 (MQ=255)

                                                                  ttGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTc                       >  1:1209528/1‑93 (MQ=255)

                                                                   tGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCg                      <  2:4319770/93‑1 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     <  2:3015124/93‑1 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     <  2:143688/93‑1 (MQ=255)

                                                                     aTCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAt                    >  2:27538/1‑93 (MQ=255)

                                                                      tCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATa                   <  2:3958229/93‑1 (MQ=255)

                                                                      tCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATa                   >  2:2128416/1‑93 (MQ=255)

                                                                      tCCAGCATCCCAGCGAGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATa                   >  1:3051415/1‑93 (MQ=255)

                                                                       ccAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAg                  <  1:1120175/93‑1 (MQ=255)

                                                                          gCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcgc               >  2:3780024/1‑93 (MQ=255)

                                                                          gCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcgc               <  2:676917/93‑1 (MQ=255)

                                                                          gCATCCCAGCGCGACTCACGTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcgc               <  1:1568003/93‑1 (MQ=255)

                                                                           cATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCt              >  1:4334269/1‑93 (MQ=255)

                                                                           cATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCt              <  1:4749848/93‑1 (MQ=255)

                                                                            aTCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTa             <  1:2236553/93‑1 (MQ=255)

                                                                            aTCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTa             >  2:1972010/1‑93 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            <  1:2540686/93‑1 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            >  1:3805109/1‑93 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            >  1:4694156/1‑93 (MQ=255)

                                                                                 cgcgcGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        <  2:1616605/92‑1 (MQ=255)

                                                                                 aGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        >  1:1879671/1‑93 (MQ=255)

                                                                                 aGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        <  1:2767585/93‑1 (MQ=255)

                                                                                  gcgcGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCg       >  1:812457/1‑93 (MQ=255)

                                                                                    gcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgcc     >  2:2449765/1‑93 (MQ=255)

                                                                                    gcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgcc     >  2:4684665/1‑93 (MQ=255)

                                                                                    gcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgcc     <  1:198290/93‑1 (MQ=255)

                                                                                      gACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgccag   >  2:164248/1‑93 (MQ=255)

                                                                                       actcacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgccagc  >  1:2574234/1‑93 (MQ=255)

                                                                                       actcacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgccagc  >  1:3025540/1‑93 (MQ=255)

GTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGC  >  NC_000913/1230053‑1230232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`