BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,243,418	T→C	V111V (GTA→GTG)	ycgR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,243,418	0	T	C	98.7%	494.3 / ‑2.8	157	V111V (GTA→GTG)	ycgR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (83/72); total (85/72)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 163 total aligned reads displayed.

CGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGA  >  NC_000913/1243326‑1243506

gggAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGt                                                                                          <  1:4015961/92‑1 (MQ=255)

  gAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCAc                                                                                        >  1:2142171/1‑93 (MQ=255)

  gAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCAc                                                                                        >  2:1102280/1‑93 (MQ=255)

    aacgtaacgtACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACaa                                                                                      >  2:3205756/1‑93 (MQ=255)

    aacgtaacgtACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACaa                                                                                      >  1:1486612/1‑93 (MQ=255)

      cgtaacgtaCTGTGATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAAc                                                                                    <  1:4667560/93‑1 (MQ=255)

        taacgtaCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCa                                                                                  <  2:3405032/93‑1 (MQ=255)

          acgtaCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATa                                                                                <  1:2951935/93‑1 (MQ=255)

          acgtaCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATa                                                                                >  2:738218/1‑93 (MQ=255)

          acgtaCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATa                                                                                >  1:1088456/1‑93 (MQ=255)

            gtaCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAg                                                                              >  2:283644/1‑93 (MQ=255)

             taCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAgg                                                                             >  2:1326571/1‑93 (MQ=255)

             taCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAgg                                                                             >  1:84719/1‑93 (MQ=255)

             taCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAAATGCGGAAATATCGGCGTCGTTGCACAAACCATAAgg                                                                             >  1:3938037/1‑93 (MQ=255)

              aCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGt                                                                            >  1:4361853/1‑93 (MQ=255)

              aCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGt                                                                            >  1:4361852/1‑93 (MQ=255)

              aCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGt                                                                            <  1:3126300/93‑1 (MQ=255)

                 gTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTggg                                                                         >  2:3171806/1‑93 (MQ=255)

                  ttATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGa                                                                        >  2:2158183/1‑93 (MQ=255)

                  ttATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGa                                                                        <  2:1946507/93‑1 (MQ=255)

                  ttATCCGCCAGTTTGGTCTGACAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGa                                                                        <  1:4406863/93‑1 (MQ=255)

                    aTCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAgg                                                                      >  2:3410283/1‑93 (MQ=255)

                       cGCCAGTTTGGTCTGGCAAAAATAGGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcgg                                                                   <  2:4215444/93‑1 (MQ=255)

                        gCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggt                                                                  >  1:2088086/1‑93 (MQ=255)

                        gCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggt                                                                  >  1:3230337/1‑93 (MQ=255)

                        gCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggt                                                                  >  1:3389525/1‑93 (MQ=255)

                        gCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGGGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggt                                                                  >  1:2632920/1‑93 (MQ=255)

                          cAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggtac                                                                <  1:1442608/93‑1 (MQ=255)

                           aGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggtacg                                                               <  2:369637/93‑1 (MQ=255)

                              ttGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGcggtacggta                                                            <  2:1162848/93‑1 (MQ=255)

                               tagTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGtaa                                                           <  2:4127779/91‑1 (MQ=255)

                               tGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGtaa                                                           <  2:1907085/93‑1 (MQ=255)

                               tGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGtaa                                                           <  2:1299015/93‑1 (MQ=255)

                                 gTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGtaata                                                         <  1:3331725/93‑1 (MQ=255)

                                 gTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGtaata                                                         <  1:2808813/93‑1 (MQ=255)

                                   cTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATaaa                                                       <  2:1999999/93‑1 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      >  1:4310566/1‑93 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      >  1:2109013/1‑93 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      >  2:4423094/1‑93 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      <  2:2150826/93‑1 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      >  2:3039518/1‑93 (MQ=255)

                                    tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAAt                                                      >  1:3025583/1‑93 (MQ=255)

                                      gCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGc                                                    >  2:8391/1‑93 (MQ=255)

                                        aaaaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCg                                                  <  1:2450596/93‑1 (MQ=255)

                                         aaaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCgg                                                 <  2:497509/93‑1 (MQ=255)

                                         aaaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCgg                                                 >  1:2407594/1‑93 (MQ=255)

                                          aaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGa                                                >  2:2559748/1‑93 (MQ=255)

                                          aaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGa                                                <  1:1891040/93‑1 (MQ=255)

                                            aTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAg                                              <  1:425335/93‑1 (MQ=255)

                                             tAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGc                                             >  1:2202402/1‑93 (MQ=255)

                                             tAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGc                                             >  1:3037580/1‑93 (MQ=255)

                                              aaGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCt                                            <  1:3700746/93‑1 (MQ=255)

                                              aaGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCt                                            <  1:1182264/93‑1 (MQ=255)

                                               aGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTg                                           <  1:30097/93‑1 (MQ=255)

                                                  cGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCaa                                        <  1:1683772/93‑1 (MQ=255)

                                                  cGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCaa                                        <  1:3356733/93‑1 (MQ=255)

                                                   ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAg                                       <  2:1438130/93‑1 (MQ=255)

                                                   ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAg                                       <  2:1968910/93‑1 (MQ=255)

                                                   ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAg                                       >  2:376699/1‑93 (MQ=255)

                                                     aTGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTa                                     <  1:4235090/93‑1 (MQ=255)

                                                     aTGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTa                                     <  2:581945/93‑1 (MQ=255)

                                                       ggAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTAtt                                   >  1:239594/1‑93 (MQ=255)

                                                         aGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACTATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCa                                 <  2:562157/93‑1 (MQ=255)

                                                          gTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCAc                                <  1:1789968/93‑1 (MQ=255)

                                                          gTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCAc                                >  2:2760859/1‑93 (MQ=255)

                                                            ggggCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCActc                              <  2:3019889/93‑1 (MQ=255)

                                                             gggCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCActct                             >  1:1607258/1‑93 (MQ=255)

                                                             gggCGGAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCActct                             <  1:588758/93‑1 (MQ=255)

                                                                 ggAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTctgctg                         >  2:3996917/1‑93 (MQ=255)

                                                                 ggAGATGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTctgctg                         >  2:450952/1‑93 (MQ=255)

                                                                    gctGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAg                      <  1:4468215/91‑1 (MQ=255)

                                                                    gaTGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAg                      >  1:2471261/1‑93 (MQ=255)

                                                                      tGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAgtt                    >  1:3515987/1‑93 (MQ=255)

                                                                      tGCGGAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAgtt                    >  1:1063580/1‑93 (MQ=255)

                                                                          gAAATATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTc                >  1:2375395/1‑93 (MQ=255)

                                                                           aaaTATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCa               <  2:2508722/93‑1 (MQ=255)

                                                                           aaaTATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCa               >  2:4251982/1‑93 (MQ=255)

                                                                           aaaTATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCa               <  2:1566793/93‑1 (MQ=255)

                                                                           aaaTATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCa               >  1:4150240/1‑93 (MQ=255)

                                                                            aaTATCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCaa              >  1:3989298/1‑93 (MQ=255)

                                                                              tatCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACa            >  1:3591397/1‑93 (MQ=255)

                                                                              tatCGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACa            >  2:1573333/1‑93 (MQ=255)

                                                                                 cGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTa         <  2:2868969/93‑1 (MQ=255)

                                                                                 cGGCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTa         <  1:4557337/93‑1 (MQ=255)

                                                                                  ggCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaa        >  1:2849025/1‑93 (MQ=255)

                                                                                  ggCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaa        <  2:3773463/93‑1 (MQ=255)

                                                                                  ggCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaa        >  2:1910493/1‑93 (MQ=255)

                                                                                  ggCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaa        >  2:2325124/1‑93 (MQ=255)

                                                                                  ggCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaa        <  2:2628178/93‑1 (MQ=255)

                                                                                   gCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaaa       >  2:973016/1‑93 (MQ=255)

                                                                                   gCGTCGTTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTaaa       <  2:118986/93‑1 (MQ=255)

                                                                                    ggtcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAAc      <  1:901148/92‑1 (MQ=255)

                                                                                     gtcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACt     >  2:3213745/1‑93 (MQ=255)

                                                                                     gtcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACt     <  2:293112/93‑1 (MQ=255)

                                                                                     gtcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACt     >  2:2734221/1‑93 (MQ=255)

                                                                                     gtcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGCAGTTGTTCAACAGTAAAcc     >  1:2162033/1‑92 (MQ=255)

                                                                                      tcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTc    <  2:1593444/93‑1 (MQ=255)

                                                                                      tcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTc    >  1:1240180/1‑93 (MQ=255)

                                                                                      tcgtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGGTGTTCAACAGTAAACTc    <  1:4130810/93‑1 (MQ=255)

                                                                                        gtTGCACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGa  >  1:823855/1‑93 (MQ=255)

CGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGA  >  NC_000913/1243326‑1243506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`