BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,273,390	T→C	R81R (CGT→CGC)	ychP →	putative factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,273,390	0	T	C	98.3%	546.2 / ‑1.5	173	R81R (CGT→CGC)	ychP	putative factor
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (62/108); total (63/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 178 total aligned reads displayed.

ATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273302‑1273479

 tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCATCGCCACCCGCGGaac                                                                                      >  2:954128/1‑92 (MQ=255)

 tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAg                                                                                      <  2:1668261/93‑1 (MQ=255)

  cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGc                                                                                     <  1:38117/93‑1 (MQ=255)

  cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGc                                                                                     <  1:2827094/93‑1 (MQ=255)

  cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGc                                                                                     <  2:456709/93‑1 (MQ=255)

  cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTAGCGGAAGc                                                                                     >  1:1743073/1‑93 (MQ=255)

   aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCt                                                                                    <  1:3629678/93‑1 (MQ=255)

   aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCt                                                                                    <  1:972630/93‑1 (MQ=255)

     cACGTAGAGTCCTGTCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTgg                                                                                  <  2:3285674/93‑1 (MQ=255)

       cGTAGAGACCGAGCTATCACCGTGGGGGAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGtt                                                                                <  2:1265619/93‑1 (MQ=255)

        gTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGttt                                                                               <  1:4260356/93‑1 (MQ=255)

          agagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgt                                                                             <  2:3815222/93‑1 (MQ=255)

          agagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgt                                                                             >  2:2865569/1‑93 (MQ=255)

          agagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgt                                                                             >  1:2382938/1‑93 (MQ=255)

           gagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgtg                                                                            <  1:1469228/93‑1 (MQ=255)

              gccTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCg                                                                         <  1:4531961/92‑1 (MQ=255)

               ccTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGt                                                                        <  1:579406/93‑1 (MQ=255)

               ccTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGt                                                                        <  1:3028689/93‑1 (MQ=255)

                cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       <  1:3957102/93‑1 (MQ=255)

                cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       <  1:698700/93‑1 (MQ=255)

                 tGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTa                                                                      <  2:3414194/93‑1 (MQ=255)

                 tGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTa                                                                      <  1:4157773/93‑1 (MQ=255)

                   gCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACa                                                                    <  2:1201406/93‑1 (MQ=255)

                     tATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGCGCCGTTACAAg                                                                  <  1:413343/93‑1 (MQ=255)

                      aTCACCGTGGGGAAATGCCAGTTTTCAAGTCAAAGTGGATAACGAAGAACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 <  2:135939/93‑1 (MQ=255)

                      aTCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 >  1:2464944/1‑93 (MQ=255)

                       tCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGaa                                                                >  1:4360795/1‑92 (MQ=255)

                       tCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGaa                                                                >  1:1178531/1‑92 (MQ=255)

                       tCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGAt                                                                <  2:2584366/93‑1 (MQ=255)

                       tCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGAt                                                                <  1:122716/93‑1 (MQ=255)

                       tCACCGTGGGGAAATGCCAGTGTTGACGACAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGGCGTTACAAGAt                                                                >  2:4630836/1‑93 (MQ=255)

                        cACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATa                                                               <  2:225562/93‑1 (MQ=255)

                        cACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATa                                                               >  2:908738/1‑93 (MQ=255)

                         aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              >  1:453491/1‑93 (MQ=255)

                         aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              <  1:933660/93‑1 (MQ=255)

                          ccGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAAt                                                             <  1:2380137/93‑1 (MQ=255)

                          ccGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAAt                                                             >  2:467967/1‑93 (MQ=255)

                            gTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAATCTGGTTTGTGCCGTTACAAGATAATGa                                                           >  1:3695102/1‑93 (MQ=255)

                              ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         <  1:1019028/93‑1 (MQ=255)

                              ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         <  2:741022/93‑1 (MQ=255)

                               gggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCg                                                        >  2:2662750/1‑93 (MQ=255)

                               gggAAACGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCg                                                        <  1:1560494/93‑1 (MQ=255)

                                ggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGt                                                       >  2:1992337/1‑93 (MQ=255)

                                ggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGt                                                       <  2:2551718/93‑1 (MQ=255)

                                 gAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGtt                                                      <  1:2982123/93‑1 (MQ=255)

                                 gAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGtt                                                      >  1:1820222/1‑93 (MQ=255)

                                  aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:3837825/93‑1 (MQ=255)

                                  aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:3082037/93‑1 (MQ=255)

                                  aaaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTa                                                     <  1:1331905/93‑1 (MQ=255)

                                   aaTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAt                                                    >  2:941757/1‑93 (MQ=255)

                                    aTGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtc                                                   >  1:692673/1‑93 (MQ=255)

                                     tGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtct                                                  <  2:3678422/93‑1 (MQ=255)

                                      gCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtctc                                                 <  1:1593086/93‑1 (MQ=255)

                                         aGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCAcc                                              >  1:107678/1‑93 (MQ=255)

                                         aGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCAcc                                              <  2:4116157/93‑1 (MQ=255)

                                          gtgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCt                                             <  2:185276/93‑1 (MQ=255)

                                          gtgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCt                                             >  2:1358956/1‑93 (MQ=255)

                                           tgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTg                                            >  2:1316038/1‑93 (MQ=255)

                                            gtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTgg                                           >  2:3541760/1‑93 (MQ=255)

                                             ttGACGTCGAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGCTAATGATCGTTATCTCACCTGGa                                          <  1:3611434/93‑1 (MQ=255)

                                             ttGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGa                                          <  2:331551/93‑1 (MQ=255)

                                             ttGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGa                                          <  2:331550/93‑1 (MQ=255)

                                             ttGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGa                                          <  2:2964042/93‑1 (MQ=255)

                                              tGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAg                                         <  2:860385/93‑1 (MQ=255)

                                              tGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAg                                         <  1:3139113/93‑1 (MQ=255)

                                              tGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAg                                         >  1:1139415/1‑93 (MQ=255)

                                                aCGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGcc                                       <  1:5320/93‑1 (MQ=255)

                                                aCGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGcc                                       <  2:4231071/93‑1 (MQ=255)

                                                 cGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCa                                      <  1:1860004/93‑1 (MQ=255)

                                                 cGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCa                                      <  1:4142346/93‑1 (MQ=255)

                                                 cGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCa                                      <  2:2296139/93‑1 (MQ=255)

                                                  gTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAg                                     <  2:1971645/93‑1 (MQ=255)

                                                   tCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGc                                    >  1:500664/1‑93 (MQ=255)

                                                     aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:2137852/93‑1 (MQ=255)

                                                     aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:350842/93‑1 (MQ=255)

                                                     aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCCGGAGCCAGCtt                                  >  2:647086/1‑93 (MQ=255)

                                                       aGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTgg                                >  1:3998742/1‑93 (MQ=255)

                                                       aGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTgg                                <  1:2265803/93‑1 (MQ=255)

                                                       aGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTgg                                <  2:1538940/93‑1 (MQ=255)

                                                          ggATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCt                             <  1:912035/93‑1 (MQ=255)

                                                           gATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCtt                            >  2:2183889/1‑93 (MQ=255)

                                                            aTAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTa                           >  1:3573570/1‑93 (MQ=255)

                                                             tAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATCATCGTTATCTCACCTGGAGCCAGCTTGGCCTTAc                          >  1:792963/1‑93 (MQ=255)

                                                              aaCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACt                         <  1:270359/93‑1 (MQ=255)

                                                              aaCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACt                         <  1:270360/93‑1 (MQ=255)

                                                               aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGTTCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTc                        >  1:40258/1‑93 (MQ=255)

                                                               aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTc                        >  2:1251272/1‑93 (MQ=255)

                                                               aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGGTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACta                        >  2:845752/1‑92 (MQ=255)

                                                                   aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    >  2:1632607/1‑93 (MQ=255)

                                                                   aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    <  2:1729945/93‑1 (MQ=255)

                                                                   aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGGTACAAGATAATGATCGTTATCTCTCCTGGAGCCAGCTTGGTCTTACTcagca                    >  2:2843161/1‑93 (MQ=255)

                                                                    ggACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagcag                   >  2:724768/1‑93 (MQ=255)

                                                                      aCATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGa                 <  1:1248367/93‑1 (MQ=255)

                                                                       cATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGAt                <  2:1434526/93‑1 (MQ=255)

                                                                         tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              <  1:1897966/93‑1 (MQ=255)

                                                                          ttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAAt             <  1:2573144/93‑1 (MQ=255)

                                                                            cACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATgg           >  1:1154418/1‑93 (MQ=255)

                                                                             aCCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATggg          <  2:2096614/93‑1 (MQ=255)

                                                                             aCCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATggg          <  1:1924161/93‑1 (MQ=255)

                                                                                  cAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGt     >  1:3896884/1‑93 (MQ=255)

ATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273302‑1273479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`