BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,543	T→C	F27L (TTT→CTT)	hnr →	Hnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,543	0	T	C	98.6%	450.0 / ‑3.4	142	F27L (TTT→CTT)	hnr	Hnr
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (86/54); total (87/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 146 total aligned reads displayed.

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGAT  >  NC_000913/1289453‑1289631

aGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGctt                                                                                        >  1:1620604/1‑93 (MQ=255)

 ggggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGcttt                                                                                       >  1:3896165/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                      >  2:390335/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                      >  1:4479372/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                      >  1:4479373/1‑93 (MQ=255)

   ggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTcc                                                                                     >  1:3180142/1‑93 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                    <  1:4252814/93‑1 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCCCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                    >  2:2274499/1‑93 (MQ=255)

     agagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTc                                                                                   <  1:2187637/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                  >  1:1174135/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                  <  2:3968626/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                  >  1:4129153/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                  <  1:2221251/93‑1 (MQ=255)

       agaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAt                                                                                 <  1:727119/93‑1 (MQ=255)

         aaCATGAAGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                               <  1:492897/93‑1 (MQ=255)

          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                              <  2:4012324/93‑1 (MQ=255)

           cATGACGCAGCCATTGGTCGGTAAACTGATTCTCATTTTTGAAGATGAGCATGTTTGTCTCTCGCTGCTGGATTCATGGCTTTCCTCATTtgg                                                                             >  2:4216609/1‑93 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                             <  2:2606873/93‑1 (MQ=255)

            aTGACGCAGCGATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGa                                                                            <  2:608867/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                           <  2:2413451/93‑1 (MQ=255)

              gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGc                                                                          >  1:3357071/1‑93 (MQ=255)

                cGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGa                                                                        <  2:3137307/93‑1 (MQ=255)

                 gctgCCATTGGTCGGAAAACAGATTCTCAGTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGAc                                                                       <  2:2246039/90‑1 (MQ=255)

                 gCAGCCATTGGGAGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGAATTTCGCTCGCTTCTCGATTCATAGCTTTCCTCATTGGGAGCGag                                                                       >  2:19133/1‑92 (MQ=255)

                  cAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACa                                                                      >  2:725993/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                     >  1:1150478/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                     >  2:4000466/1‑93 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                  >  1:2165237/1‑93 (MQ=255)

                        ttGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTa                                                                <  1:1490822/93‑1 (MQ=255)

                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                              <  1:2027380/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                             >  1:92809/1‑93 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                             <  1:1704982/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                             >  1:92810/1‑93 (MQ=255)

                            tCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTgg                                                            >  1:2995732/1‑93 (MQ=255)

                              ggAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcg                                                          >  1:3498840/1‑93 (MQ=255)

                              ggAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcg                                                          >  2:2035487/1‑93 (MQ=255)

                              ggAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcg                                                          >  1:3089357/1‑93 (MQ=255)

                               gAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcgg                                                         >  1:1419293/1‑93 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGTTACTggcggc                                                        <  1:1367604/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                        <  2:3180142/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                        <  1:597558/93‑1 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                       <  2:4546533/93‑1 (MQ=255)

                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                      >  2:3898326/1‑93 (MQ=255)

                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                      >  2:2843157/1‑93 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                     >  2:413207/1‑93 (MQ=255)

                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                    >  1:962552/1‑93 (MQ=255)

                                     aGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATg                                                   >  2:3665311/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                  <  1:4608403/93‑1 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                  >  1:3760933/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                  >  2:4707329/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                  >  1:4284668/1‑93 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                <  2:436677/93‑1 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                               <  2:3092860/93‑1 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                               <  1:1575494/93‑1 (MQ=255)

                                           tcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTgg                                             >  1:4455782/1‑93 (MQ=255)

                                             aTTGTTGAAGATGAGCAGGTATTTCGATCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt                                           <  1:4202985/93‑1 (MQ=255)

                                              ttgttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATg                                          >  2:1905741/1‑93 (MQ=255)

                                               tgttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGc                                         <  2:741083/93‑1 (MQ=255)

                                                 ttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGccc                                       >  2:4310815/1‑93 (MQ=255)

                                                      gATGAGCAGGTATTTCGCTCGCTTCTGGATTAATGGCTTTCCTCATTGGGAGCGCAAACGGTACTGGAGGCTGATGGGGGTGATCACCTTgcg                                  >  1:3126088/1‑91 (MQ=255)

                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                <  2:3784296/93‑1 (MQ=255)

                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                >  2:4662518/1‑93 (MQ=255)

                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                <  2:2403142/93‑1 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTg                               >  1:2903753/1‑93 (MQ=255)

                                                           gCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTgtt                             >  2:1721809/1‑91 (MQ=255)

                                                           gCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCt                             >  1:1157737/1‑93 (MQ=255)

                                                           gCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCt                             >  1:4385072/1‑93 (MQ=255)

                                                           gCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCt                             <  2:463446/93‑1 (MQ=255)

                                                           gCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCt                             <  1:2379317/93‑1 (MQ=255)

                                                            cAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACAGTACTGGCGGCTGATGGGGTGGATGACCTTGAGATGCTg                            >  2:4458454/1‑93 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                           <  2:11199/93‑1 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGAAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                           >  1:4506341/1‑93 (MQ=255)

                                                                tagttCGCTCGTTGCTGTATTCATGGCTTTCCTCATTGGGAGCGACAACGGTTCTGGCGGCTGAATGGGTGGATGTCCTTGAGTTGCTGGGAg                        >  2:4208525/4‑93 (MQ=255)

                                                                tATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAg                        <  1:4005245/93‑1 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACACCGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                       >  2:3451521/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                       >  2:1902551/1‑93 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                      <  1:451361/93‑1 (MQ=255)

                                                                      gctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTcg                  >  2:4008908/1‑92 (MQ=255)

                                                                      gctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCa                  >  1:2763718/1‑93 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                 >  2:4367349/1‑93 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTCGATGCCCTTGAGTTGCTGGGAGGTTTCAc                 >  1:2761531/1‑93 (MQ=255)

                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGAGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                >  2:3819133/1‑93 (MQ=255)

                                                                         cgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTc               <  1:839244/93‑1 (MQ=255)

                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc              <  1:1309948/93‑1 (MQ=255)

                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg            >  2:1153367/1‑93 (MQ=255)

                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg            >  2:185394/1‑93 (MQ=255)

                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg            >  1:3690482/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa           <  2:1192531/93‑1 (MQ=255)

                                                                              cTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAc          <  1:4104837/93‑1 (MQ=255)

                                                                               tGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAcc         <  2:4150137/93‑1 (MQ=255)

                                                                               tGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAcc         >  1:1671144/1‑93 (MQ=255)

                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCAGACCt        >  2:2636868/1‑93 (MQ=255)

                                                                                 gATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtg       >  1:3799338/1‑93 (MQ=255)

                                                                                 gATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtg       >  1:3803043/1‑93 (MQ=255)

                                                                                 gATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtg       <  1:791/93‑1 (MQ=255)

                                                                                  aTTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtga      <  2:1457601/93‑1 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGCCCtgat     >  2:1298745/1‑93 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat     >  2:2817981/1‑93 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat     >  2:2271903/1‑93 (MQ=255)

                                                                                      aTGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCGGACATgatgat  >  2:1475550/1‑93 (MQ=255)

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGAT  >  NC_000913/1289453‑1289631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`