Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,617 T→C V232A (GTG→GCG)  oppB → oligopeptide transport permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,6170TC96.8% 373.9 / ‑0.8 124V232A (GTG→GCG) oppBoligopeptide transport permease protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (65/55);  total (67/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706
                                                                                          |                                                                                         
ccTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCgcgc                                                                                         <  1:3772522/92‑1 (MQ=255)
acTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCgcgc                                                                                         >  1:1005860/1‑93 (MQ=255)
acTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCgcgc                                                                                         >  1:721647/1‑93 (MQ=255)
   ccAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctct                                                                                      >  1:2359181/1‑93 (MQ=255)
    cAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctc                                                                                     >  2:4446913/1‑93 (MQ=255)
    cAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctc                                                                                     >  2:256031/1‑93 (MQ=255)
     aaTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctct                                                                                    >  1:107230/1‑93 (MQ=255)
     aaTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctctct                                                                                    >  2:792362/1‑93 (MQ=255)
      aTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   <  2:3019782/93‑1 (MQ=255)
        tttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtat                                                                                 <  2:1009533/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  2:3977210/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  2:2908115/93‑1 (MQ=255)
          tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTatat                                                                               <  1:4529597/93‑1 (MQ=255)
           aTTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACGCGCGCTCTCTTATATg                                                                              <  2:247/93‑1 (MQ=255)
            ttCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATgg                                                                             <  1:3584050/93‑1 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  1:1907442/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  1:1161219/1‑93 (MQ=255)
                   tGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGc                                                                      <  2:2816959/93‑1 (MQ=255)
                   tGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGc                                                                      >  1:973937/1‑93 (MQ=255)
                    gCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCa                                                                     >  2:486249/1‑93 (MQ=255)
                      ccGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCTCAtt                                                                   >  1:912955/1‑93 (MQ=255)
                        gggCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTg                                                                 <  2:2682130/93‑1 (MQ=255)
                        gggCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTg                                                                 >  2:2175182/1‑93 (MQ=255)
                          gCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTc                                                               <  1:4233067/93‑1 (MQ=255)
                              aaGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCa                                                           >  1:2212436/1‑93 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAACCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          >  1:222633/1‑93 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          <  2:152574/93‑1 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          >  1:767989/1‑93 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          <  2:869582/93‑1 (MQ=255)
                                 ggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCatta                                                        <  2:722212/93‑1 (MQ=255)
                                  gTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattat                                                       <  1:1940218/93‑1 (MQ=255)
                                  gTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattaa                                                       >  2:4227523/1‑92 (MQ=255)
                                   ttACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattatt                                                      <  1:3028944/93‑1 (MQ=255)
                                   ttACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattatt                                                      <  2:4272876/93‑1 (MQ=255)
                                   ttACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattatt                                                      >  2:4497638/1‑93 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGTAttatta                                                     >  2:2396734/1‑93 (MQ=255)
                                     aCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAc                                                    <  2:2784105/93‑1 (MQ=255)
                                     aCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAc                                                    <  1:3051105/93‑1 (MQ=255)
                                      ccTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAcc                                                   <  2:2318585/93‑1 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 <  1:398880/93‑1 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 <  2:2408229/93‑1 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACTGGt                                                <  2:3495552/93‑1 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGt                                                >  2:3049287/1‑93 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGt                                                <  2:1071825/93‑1 (MQ=255)
                                          tGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGtt                                               >  2:2955011/1‑93 (MQ=255)
                                           gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTc                                              <  1:3682272/93‑1 (MQ=255)
                                           gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTc                                              <  1:1707284/93‑1 (MQ=255)
                                           gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTc                                              >  2:596514/1‑93 (MQ=255)
                                             ggcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTa                                            <  1:4115629/93‑1 (MQ=255)
                                             ggcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTa                                            <  2:1661486/93‑1 (MQ=255)
                                              gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAt                                           >  2:1966369/1‑93 (MQ=255)
                                              gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAt                                           <  1:2013812/93‑1 (MQ=255)
                                               cggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATg                                          <  1:468050/93‑1 (MQ=255)
                                                ggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATgg                                         >  2:35149/1‑93 (MQ=255)
                                                ggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATgg                                         <  2:3526605/93‑1 (MQ=255)
                                                 gATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGt                                        >  1:4308722/1‑93 (MQ=255)
                                                   tCATTATACGTCTCGCATTAAAACCTGCTCTGTTTCCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTCTTACCGGTTCTATGGTTa                                      <  2:3101701/93‑1 (MQ=255)
                                                    cATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTAt                                     <  2:1590310/93‑1 (MQ=255)
                                                    cATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTAt                                     <  1:3248790/93‑1 (MQ=255)
                                                     aTTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATc                                    >  2:3986479/1‑93 (MQ=255)
                                                     aTTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATc                                    <  1:4675369/93‑1 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCg                                   >  1:4370857/1‑93 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCg                                   >  2:1712485/1‑93 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGACCCGCATTTGTCGGGATTATTACCGGTTCTATGGTTATCg                                   >  2:1915866/1‑93 (MQ=255)
                                                       tttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGa                                  >  2:3050838/1‑93 (MQ=255)
                                                         tACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGaaa                                <  2:3138894/93‑1 (MQ=255)
                                                         tACGTCACGCATTAAAACCTGCTCTATTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGaaa                                <  2:3138895/93‑1 (MQ=255)
                                                          aCGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAAc                               >  1:2652089/1‑93 (MQ=255)
                                                          aCGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAAc                               >  2:2487274/1‑93 (MQ=255)
                                                          aCGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAAc                               >  1:1536388/1‑93 (MQ=255)
                                                           cGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGACAcc                              >  1:1328784/1‑93 (MQ=255)
                                                            gTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCa                             >  1:4119221/1‑93 (MQ=255)
                                                                 gCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                        >  1:1028347/1‑93 (MQ=255)
                                                                 gCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                        >  2:1876409/1‑93 (MQ=255)
                                                                  cATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATg                       <  2:1828525/93‑1 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                      >  1:81014/1‑93 (MQ=255)
                                                                       aaaCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTg                  >  1:1792790/1‑93 (MQ=255)
                                                                       aaaCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTg                  >  2:557198/1‑93 (MQ=255)
                                                                         aCCTGCTCTGTTCCCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGcc                <  1:2588296/93‑1 (MQ=255)
                                                                         aCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGcc                <  1:2329014/93‑1 (MQ=255)
                                                                         aCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGcc                <  1:2792231/93‑1 (MQ=255)
                                                                          ccTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCg               >  1:1376089/1‑93 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              <  1:2774178/93‑1 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              <  1:2777651/93‑1 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              <  1:2777652/93‑1 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  2:2385160/1‑93 (MQ=255)
                                                                             gCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgggg            >  2:3206792/1‑93 (MQ=255)
                                                                             gCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgggg            >  2:4392103/1‑93 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  1:302072/93‑1 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  1:4315583/93‑1 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  2:4019/93‑1 (MQ=255)
                                                                               tctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          >  1:68587/1‑93 (MQ=255)
                                                                               tctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          >  1:3168598/1‑93 (MQ=255)
                                                                               tcagTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGACATTATTTCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          <  2:4215553/90‑1 (MQ=255)
                                                                                 tGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTg        >  1:2762369/1‑93 (MQ=255)
                                                                                   ttACCCGCGCTCTCTTATATGGGCCCCGCATTTTTCGGCATTATTACCGGGTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGa      >  1:1743429/1‑93 (MQ=255)
                                                                                   ttACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGa      >  2:301755/1‑93 (MQ=255)
                                                                                    ttcccGCGCTGTCTTATATGGGGCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGAc     <  1:4594971/91‑1 (MQ=255)
                                                                                     aCCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACa    >  2:3813937/1‑93 (MQ=255)
                                                                                       ccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAc  >  1:3195260/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑