BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,962	C→T	R194H (CGT→CAT)	sapC ←	SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,962	0	C	T	99.2%	428.5 / ‑5.2	124	R194H (CGT→CAT)	sapC	SapC
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (55/68); total (55/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 128 total aligned reads displayed.

GTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAC  >  NC_000913/1351873‑1352050

gTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATggg                                                                                       >  2:2323099/1‑93 (MQ=255)

gTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATggg                                                                                       <  1:1719347/93‑1 (MQ=255)

gTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATggg                                                                                       <  2:4461018/93‑1 (MQ=255)

   cgcgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGggcgg                                                                                    <  2:1748200/93‑1 (MQ=255)

   cgcgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGggcgg                                                                                    <  2:2244746/93‑1 (MQ=255)

     cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGgcggcg                                                                                  >  1:1093425/1‑93 (MQ=255)

      gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGa                                                                                 >  2:4155422/1‑93 (MQ=255)

       ggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAt                                                                                <  1:3081265/93‑1 (MQ=255)

       ggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAt                                                                                >  2:2590766/1‑93 (MQ=255)

       ggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAt                                                                                <  2:3769271/93‑1 (MQ=255)

         tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATaa                                                                              <  2:422886/93‑1 (MQ=255)

          gATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAAc                                                                             <  2:2651920/93‑1 (MQ=255)

          gATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAAc                                                                             >  2:4241158/1‑93 (MQ=255)

           aTCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACg                                                                            >  1:2404437/1‑93 (MQ=255)

               cGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAct                                                                        >  2:4537370/1‑93 (MQ=255)

                ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActc                                                                       <  2:486829/93‑1 (MQ=255)

                 gTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActct                                                                      >  2:2325497/1‑93 (MQ=255)

                   gACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCttt                                                                    <  2:3992823/93‑1 (MQ=255)

                   gACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCttt                                                                    >  2:2867669/1‑93 (MQ=255)

                   gACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGATGATGCGCCATCCAGATGTGCGGCGATAACGTACTCttt                                                                    >  1:3001846/1‑93 (MQ=255)

                     ccAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCttttt                                                                  >  2:2226466/1‑93 (MQ=255)

                     ccAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCttttt                                                                  >  2:3962929/1‑93 (MQ=255)

                       aGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTcc                                                                <  1:666407/93‑1 (MQ=255)

                        gCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                               >  1:4262092/1‑93 (MQ=255)

                        gCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                               <  2:1318820/93‑1 (MQ=255)

                        acccGGCGGTGAGGTTTGGCATCACAGCAAACCAGAGAAGATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                               <  2:1318819/92‑1 (MQ=255)

                          ccGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGt                                                             <  2:1659994/93‑1 (MQ=255)

                          ccGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGt                                                             >  2:1949141/1‑93 (MQ=255)

                          ccGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGt                                                             <  1:4669625/93‑1 (MQ=255)

                          ccGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGCACTCTTTTTCCAGt                                                             <  1:3685029/93‑1 (MQ=255)

                           cggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGtt                                                            >  2:863246/1‑93 (MQ=255)

                            ggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTc                                                           <  2:4322714/93‑1 (MQ=255)

                               ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                        <  2:2336859/93‑1 (MQ=255)

                               ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                        >  1:1005630/1‑93 (MQ=255)

                                gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                       <  1:1383058/93‑1 (MQ=255)

                                 tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                      >  2:4565241/1‑93 (MQ=255)

                                 tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                      <  1:2280187/93‑1 (MQ=255)

                                 tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                      <  2:3541478/93‑1 (MQ=255)

                                 tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                      <  2:3487281/93‑1 (MQ=255)

                                  gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATg                                                     >  2:2031624/1‑93 (MQ=255)

                                  gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATg                                                     >  1:3014511/1‑93 (MQ=255)

                                  gATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATg                                                     >  2:2043279/1‑93 (MQ=255)

                                   aTGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGc                                                    >  1:1067346/1‑93 (MQ=255)

                                   aTGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGc                                                    >  1:4107226/1‑93 (MQ=255)

                                     gTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                                                  >  1:529578/1‑93 (MQ=255)

                                       ttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCa                                                <  1:3733471/93‑1 (MQ=255)

                                        tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAt                                               >  1:338893/1‑93 (MQ=255)

                                         ggCGTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGTCGGCGATAACGTCCTCTTTTTCCAGTTCGTCATGCACCATg                                              <  1:475216/93‑1 (MQ=255)

                                          gCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGc                                             <  1:932337/93‑1 (MQ=255)

                                             tCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGt                                          <  1:1943859/93‑1 (MQ=255)

                                             tCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGt                                          >  1:4741272/1‑93 (MQ=255)

                                              cacaGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTa                                         <  1:2800267/93‑1 (MQ=255)

                                                  gCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATc                                     >  2:3126358/1‑93 (MQ=255)

                                                  gCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATc                                     <  1:3377738/93‑1 (MQ=255)

                                                   cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                    <  2:1875576/93‑1 (MQ=255)

                                                    aaaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGa                                   >  2:3698915/1‑93 (MQ=255)

                                                     aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                  <  2:2794481/93‑1 (MQ=255)

                                                     aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                  <  2:2659026/93‑1 (MQ=255)

                                                      aCCAGAGAATATTCAGCGTTGATGCGCCATCCGGATGGGCGGCGATAACGCACTCTTTTTCAAGTTCGTCATCCACCATGCTTTACATCGacc                                 >  2:3299032/1‑91 (MQ=255)

                                                      aCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAc                                 <  1:3656379/93‑1 (MQ=255)

                                                      aCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAc                                 <  1:269483/93‑1 (MQ=255)

                                                       ccAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACg                                >  2:107495/1‑93 (MQ=255)

                                                        cAGTGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGt                               <  2:1860060/93‑1 (MQ=255)

                                                        cAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTTTTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGt                               <  2:2966826/93‑1 (MQ=255)

                                                        cAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGt                               >  2:574060/1‑93 (MQ=255)

                                                        cAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGt                               >  2:560376/1‑93 (MQ=255)

                                                         agagAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTa                              <  1:2013605/93‑1 (MQ=255)

                                                         agagAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGCTCGTCATGCACCATGCTGTAAATCGAACGTa                              <  1:1921301/93‑1 (MQ=255)

                                                          gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                             <  2:4204451/93‑1 (MQ=255)

                                                          gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                             >  2:3745743/1‑93 (MQ=255)

                                                           agaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATTGAAATTAcc                            >  2:3356288/1‑93 (MQ=255)

                                                            gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                           >  1:2120917/1‑93 (MQ=255)

                                                             aaTATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAt                          >  1:2535487/1‑93 (MQ=255)

                                                              atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                         <  1:704311/93‑1 (MQ=255)

                                                               tatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAc                        <  1:1729638/93‑1 (MQ=255)

                                                                    aGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgc                   <  2:2657119/93‑1 (MQ=255)

                                                                     gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATTCACCATGCTGTAAATCGAACGTACCATACGCGgca                  <  2:1548039/93‑1 (MQ=255)

                                                                     gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                  >  2:1389980/1‑93 (MQ=255)

                                                                     gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                  >  1:170390/1‑93 (MQ=255)

                                                                          gATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAt             <  1:4304385/93‑1 (MQ=255)

                                                                            tGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgc           <  2:2078386/93‑1 (MQ=255)

                                                                              cgcCATCCAGATGGGCGGCGATACCGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgcca         <  2:2609488/93‑1 (MQ=255)

                                                                              cgcCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgcca         <  2:2934112/93‑1 (MQ=255)

                                                                              cgcCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgcca         <  1:1485283/93‑1 (MQ=255)

                                                                              cgcCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgcca         <  2:4656788/93‑1 (MQ=255)

                                                                              cgcCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgcca         <  1:1151858/93‑1 (MQ=255)

                                                                               gcCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccag        <  1:202550/93‑1 (MQ=255)

                                                                                ccATCCAGATGGGCGGCTATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagc       <  2:4371575/93‑1 (MQ=255)

                                                                                ccATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagc       >  1:3195699/1‑93 (MQ=255)

                                                                                  ctCCAGATGGGCGGCGATAACGCTCCTTGTGTCCAGGTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca     <  1:1367458/92‑1 (MQ=255)

                                                                                  aTCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca     >  1:2545075/1‑93 (MQ=255)

                                                                                   tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag    >  1:2227628/1‑93 (MQ=255)

                                                                                   tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag    >  1:3362346/1‑93 (MQ=255)

                                                                                   tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag    >  1:3756851/1‑93 (MQ=255)

                                                                                   tCCAGATGAGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag    <  2:4214229/93‑1 (MQ=255)

                                                                                    ccAGATGGGCGGCGATAACGTGCTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAAGCGAACGTACCATACGCGGCAGCAATGCCAGCCAGa   <  1:636425/93‑1 (MQ=255)

                                                                                    ccAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGa   <  2:1050644/93‑1 (MQ=255)

                                                                                     cAGATGGGTGGTGAAAACGTACTCTTTTTTCAGTTCGGCATGCACTATGCAGTAAATCAAAAGCACCATACGAGTCAGGAGTgctagccactc  >  2:2362937/1‑90 (MQ=255)

                                                                                     cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc  >  1:724956/1‑93 (MQ=255)

                                                                                     cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc  <  2:24643/93‑1 (MQ=255)

GTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAC  >  NC_000913/1351873‑1352050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`